LOCUS       RC229064                8250 bp    DNA     circular  SYN 
DEFINITION  PALLD (Myc-DDK-tagged)-Human palladin, cytoskeletal associated protein (PALLD), transcript variant 1
ACCESSION   NM_001166108
VERSION     NM_001166108.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4407..4498 
                     /label="Myc-DDK"  
     misc_feature    4528..5113 
                     /label="PolyA signal"  
     misc_feature    4585..4604 
                     /label="Restriction Enzyme"  
     misc_feature    5389..6061 
                     /label="ColE1"  
     misc_feature    6491..7285 
                     /label="Kan/Neo"  
     misc_feature    7320..7622 
                     /label="SV40 ori"  
     misc_feature    7708..8185 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4389..4395 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4389 
                     /label="PALLD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGGGACC TCCTCCCATG AGTCCTTCTA TGACTCCCTC TCAGACATGC 
     1081 AGGAAGAAAG CAAGAATACT GACTTCTTCC CGGGCCTTTC TGCTTTCCTC AGCCAGGAAG 
     1141 AGATAAACAA GAGTCTTGAC CTGGCCCGGA GAGCCATAGC CGACTCCGAA ACAGAAGATT 
     1201 TTGACTCGGA AAAGGAGATC TCGCAGATTT TCAGTACTTC TCCTGCAAGC CTCTGTGAAC 
     1261 ATCCTTCCCA TAAGGAGACC AAATTGGGTG AACACGCCTC GAGGAGACCT CAGGATAACA 
     1321 GGTCAACACC TGTCCAGCCT CTGGCAGAGA AACAAACTAA GAGTATCTCT TCACCTGTTT 
     1381 CAAAGAGGAA ACCTGCCATG TCACCCCTGC TCACCAGGCC CAGCTACATC CGGAGCCTCC 
     1441 GAAAGGCTGA AAAGCGTGGT GCAAAAACTC CCAGCACAAA CGTAAAGCCC AAAACGCCAC 
     1501 ATCAAAGAAA GGGTGGCCCC CAGAGCCAGC TGTGTGACAA GGCAGCTAAT TTAATTGAGG 
     1561 AGCTAACATC CATATTTAAA GCCGCAAAGC CAAGAAACAG AAGCCCAAAT GGGGAGTCCT 
     1621 CGTCACCAGA CAGTGGGTAC CTGTCTCCTA AAAATCAGCC GTCAGCCCTG CTGAGTGCCT 
     1681 CAGCCAGCCA GAGCCCTATG GAAGACCAAG GGGAGATGGA AAGAGAGGTC AAGTCCCCTG 
     1741 GGGCCAGGCA TTGCTACCAG GACAACCAGG ACTTGGCAGT GCCACACAAC CGCAAGTCTC 
     1801 ACCCACAGCC CCACAGCGCC CTCCACTTCC CAGCTGCACC TCGATTCATC CAAAAGCTGA 
     1861 GGAGCCAAGA AGTAGCAGAA GGGAGCCGAG TTTATCTGGA GTGTAGAGTC ACTGGAAACC 
     1921 CCACTCCTCG AGTCAGATGG TTCTGTGAAG GGAAAGAACT GCACAACACT CCTGATATTC 
     1981 AAATCCACTG TGAGGGAGGG GACCTCCATA CCCTGATCAT AGCAGAGGCC TTTGAGGACG 
     2041 ACACAGGTCG CTACACCTGT TTGGCTACGA ATCCCAGCGG CTCAGACACA ACATCTGCTG 
     2101 AGGTGTTCAT TGAAGGTGCC AGTTCAACAG ATTCTGACAG TGAAAGTTTA GCTTTCAAAT 
     2161 CAAGAGCTGG AGCTATGCCA CAAGCTCAAA AGAAAACAAC TTCTGTTTCC TTGACAATAG 
     2221 GATCATCATC TCCAAAGACA GGGGTGACCA CAGCTGTGAT TCAACCACTG TCTGTCCCTG 
     2281 TGCAACAGGT TCACAGTCCA ACTTCATATC TCTGCCGACC TGATGGAACC ACTACTGCCT 
     2341 ACTTTCCTCC TGTTTTTACA AAGGAACTGC AAAACACAGC CGTGGCGGAA GGCCAGGTGG 
     2401 TGGTTCTGGA GTGCCGGGTC CGTGGGGCAC CCCCTCTGCA GGTCCAGTGG TTTCGGCAAG 
     2461 GGAGTGAAAT CCAAGACTCT CCAGATTTCC GAATTCTACA GAAAAAACCT AGATCTACAG 
     2521 CTGAACCTGA GGAGATTTGC ACCCTAGTTA TCGCTGAGAC TTTCCCTGAA GATGCAGGGA 
     2581 TCTTTACATG TTCAGCAAGA AATGATTATG GATCAGCAAC CAGCACTGCC CAGCTGGTTG 
     2641 TCACCTCAGC CAACACTGAA AACTGTAGTT ACGAGTCAAT GGGAGAATCC AACAATGACC 
     2701 ACTTCCAACA CTTTCCACCT CCCCCTCCAA TCTTGGAGAC AAGTTCCTTG GAGTTGGCTT 
     2761 CAAAGAAACC ATCTGAGATC CAGCAGGTGA ACAACCCTGA GTTAGGCCTG AGCAGGGCAG 
     2821 CCCTTCAAAT GCAATTCAAT GCTGCTGAGA GGGAAACGAA CGGAGTCCAT CCCAGCCGTG 
     2881 GAGTAAATGG ACTGATTAAC GGCAAAGCTA ACAGTAATAA ATCTCTTCCA ACACCAGCTG 
     2941 TCCTGCTTTC ACCCACTAAG GAGCCACCAC CTCTGCTTGC CAAACCAAAA CTAGGATTTC 
     3001 CAAAGAAGGC CAGTAGAACT GCTAGAATAG CCTCCGATGA GGAAATTCAA GGCACAAAGG 
     3061 ATGCTGTTAT TCAAGACCTG GAACGAAAAC TTCGCTTCAA GGAGGACCTC CTGAACAATG 
     3121 GCCAGCCGAG GTTAACATAC GAAGAAAGAA TGGCTCGTCG ACTGCTAGGT GCTGACAGTG 
     3181 CAACTGTCTT TAATATTCAG GAGCCAGAAG AGGAAACAGC TAATCAGGAC ATTGGTTCTC 
     3241 CTCATGCTTC TGTAGGGAGT CCTCTGGATG GTCAAAAGGA ATACAAAGTC TCCAGCTGTG 
     3301 AACAGAGACT CATCAGTGAA ATAGAGTACA GGCTAGAAAG GTCTCCTGTG GATGAATCAG 
     3361 GTGATGAAGT TCAGTATGGA GATGTGCCTG TGGAAAATGG AATGGCACCA TTCTTTGAGA 
     3421 TGAAGCTGAA ACATTACAAG ATCTTTGAGG GAATGCCAGT AACTTTCACA TGTAGAGTGG 
     3481 CTGGAAATCC AAAGCCAAAG ATCTATTGGT TTAAAGATGG GAAGCAGATC TCTCCAAAGA 
     3541 GTGATCACTA CACCATTCAA AGAGATCTCG ATGGGACCTG CTCCCTCCAT ACCACAGCCT 
     3601 CCACCCTAGA TGATGATGGG AATTATACAA TTATGGCTGC AAACCCTCAG GGCCGCATCA 
     3661 GTTGTACTGG ACGGCTAATG GTACAGGCTG TCAACCAAAG AGGTCGAAGT CCCCGGTCTC 
     3721 CCTCAGGCCA TCCTCATGTC AGAAGGCCTC GTTCTAGATC AAGGGACAGT GGAGACGAAA 
     3781 ATGAACCAAT TCAGGAGCGA TTCTTCAGAC CTCACTTCTT GCAGGCTCCT GGAGATCTGA 
     3841 CTGTTCAAGA AGGAAAACTC TGCAGAATGG ACTGCAAAGT CAGTGGGTTA CCAACCCCAG 
     3901 ATCTAAGCTG GCAACTAGAT GGAAAGCCCG TACGCCCTGA CAGTGCTCAC AAGATGCTGG 
     3961 TGCGTGAGAA CGGGGTGCAC TCTCTGATCA TAGAGCCAGT CACGTCACGT GATGCCGGCA 
     4021 TCTACACATG TATAGCTACC AACCGAGCAG GACAGAACTC ATTCAGCCTG GAGCTTGTGG 
     4081 TTGCTGCTAA AGAAGCACAC AAACCCCCTG TGTTTATTGA GAAGCTCCAA AACACAGGAG 
     4141 TTGCTGATGG GTACCCAGTG CGGCTGGAAT GTCGTGTATT GGGAGTGCCA CCACCTCAGA 
     4201 TATTTTGGAA GAAAGAAAAT GAATCACTCA CTCACAGCAC TGACCGAGTG AGCATGCACC 
     4261 AGGACAACCA CGGCTACATC TGCCTGCTCA TTCAGGGAGC CACAAAAGAA GATGCTGGGT 
     4321 GGTATACTGT GTCAGCCAAG AATGAAGCAG GGATTGTGTC CTGTACTGCC AGGCTGGACG 
     4381 TTTACATTTC TCGACATACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4441 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4501 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4561 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4621 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4681 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4741 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4801 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4861 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4921 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4981 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5041 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5101 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5161 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5221 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5281 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5341 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5401 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5461 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5521 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5581 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5641 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5701 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5761 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5821 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5881 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5941 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6001 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6061 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6121 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6181 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6241 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6301 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6361 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6421 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6481 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6541 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6601 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6661 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6721 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6781 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6841 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6901 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6961 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7021 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7081 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7141 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7201 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7261 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7321 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7381 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7441 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7501 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7621 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7681 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7741 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7801 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7861 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7921 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7981 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8041 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8101 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8161 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8221 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//