LOCUS       RC229049                7815 bp    DNA     circular  SYN 
DEFINITION  GLI1 (Myc-DDK-tagged)-Human GLI family zinc finger 1 (GLI1), transcript variant 2
ACCESSION   NM_001160045
VERSION     NM_001160045.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3972..4063 
                     /label="Myc-DDK"  
     misc_feature    4093..4678 
                     /label="PolyA signal"  
     misc_feature    4150..4169 
                     /label="Restriction Enzyme"  
     misc_feature    4954..5626 
                     /label="ColE1"  
     misc_feature    6056..6850 
                     /label="Kan/Neo"  
     misc_feature    6885..7187 
                     /label="SV40 ori"  
     misc_feature    7273..7750 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3954..3960 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3954 
                     /label="GLI1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCCATCT CTGGGATTCC CAGCCCAGAT GAATCACCAA AAAGGGCCCT 
     1081 CGCCTTCCTT TGGGGTCCAG CCTTGTGGTC CCCATGACTC TGCCCGGGGT GGGATGATCC 
     1141 CACATCCTCA GTCCCGGGGA CCCTTCCCAA CTTGCCAGCT GAAGTCTGAG CTGGACATGC 
     1201 TGGTTGGCAA GTGCCGGGAG GAACCCTTGG AAGGTGATAT GTCCAGCCCC AACTCCACAG 
     1261 GCATACAGGA TCCCCTGTTG GGGATGCTGG ATGGGCGGGA GGACCTCGAG AGAGAGGAGA 
     1321 AGCGTGAGCC TGAATCTGTG TATGAAACTG ACTGCCGTTG GGATGGCTGC AGCCAGGAAT 
     1381 TTGACTCCCA AGAGCAGCTG GTGCACCACA TCAACAGCGA GCACATCCAC GGGGAGCGGA 
     1441 AGGAGTTCGT GTGCCACTGG GGGGGCTGCT CCAGGGAGCT GAGGCCCTTC AAAGCCCAGT 
     1501 ACATGCTGGT GGTTCACATG CGCAGACACA CTGGCGAGAA GCCACACAAG TGCACGTTTG 
     1561 AAGGGTGCCG GAAGTCATAC TCACGCCTCG AAAACCTGAA GACGCACCTG CGGTCACACA 
     1621 CGGGTGAGAA GCCATACATG TGTGAGCACG AGGGCTGCAG TAAAGCCTTC AGCAATGCCA 
     1681 GTGACCGAGC CAAGCACCAG AATCGGACCC ATTCCAATGA GAAGCCGTAT GTATGTAAGC 
     1741 TCCCTGGCTG CACCAAACGC TATACAGATC CTAGCTCGCT GCGAAAACAT GTCAAGACAG 
     1801 TGCATGGTCC TGACGCCCAT GTGACCAAAC GGCACCGTGG GGATGGCCCC CTGCCTCGGG 
     1861 CACCATCCAT TTCTACAGTG GAGCCCAAGA GGGAGCGGGA AGGAGGTCCC ATCAGGGAGG 
     1921 AAAGCAGACT GACTGTGCCA GAGGGTGCCA TGAAGCCACA GCCAAGCCCT GGGGCCCAGT 
     1981 CATCCTGCAG CAGTGACCAC TCCCCGGCAG GGAGTGCAGC CAATACAGAC AGTGGTGTGG 
     2041 AAATGACTGG CAATGCAGGG GGCAGCACTG AAGACCTCTC CAGCTTGGAC GAGGGACCTT 
     2101 GCATTGCTGG CACTGGTCTG TCCACTCTTC GCCGCCTTGA GAACCTCAGG CTGGACCAGC 
     2161 TACATCAACT CCGGCCAATA GGGACCCGGG GTCTCAAACT GCCCAGCTTG TCCCACACCG 
     2221 GTACCACTGT GTCCCGCCGC GTGGGCCCCC CAGTCTCTCT TGAACGCCGC AGCAGCAGCT 
     2281 CCAGCAGCAT CAGCTCTGCC TATACTGTCA GCCGCCGCTC CTCCCTGGCC TCTCCTTTCC 
     2341 CCCCTGGCTC CCCACCAGAG AATGGAGCAT CCTCCCTGCC TGGCCTTATG CCTGCCCAGC 
     2401 ACTACCTGCT TCGGGCAAGA TATGCTTCAG CCAGAGGGGG TGGTACTTCG CCCACTGCAG 
     2461 CATCCAGCCT GGATCGGATA GGTGGTCTTC CCATGCCTCC TTGGAGAAGC CGAGCCGAGT 
     2521 ATCCAGGATA CAACCCCAAT GCAGGGGTCA CCCGGAGGGC CAGTGACCCA GCCCAGGCTG 
     2581 CTGACCGTCC TGCTCCAGCT AGAGTCCAGA GGTTCAAGAG CCTGGGCTGT GTCCATACCC 
     2641 CACCCACTGT GGCAGGGGGA GGACAGAACT TTGATCCTTA CCTCCCAACC TCTGTCTACT 
     2701 CACCACAGCC CCCCAGCATC ACTGAGAATG CTGCCATGGA TGCTAGAGGG CTACAGGAAG 
     2761 AGCCAGAAGT TGGGACCTCC ATGGTGGGCA GTGGTCTGAA CCCCTATATG GACTTCCCAC 
     2821 CTACTGATAC TCTGGGATAT GGGGGACCTG AAGGGGCAGC AGCTGAGCCT TATGGAGCGA 
     2881 GGGGTCCAGG CTCTCTGCCT CTTGGGCCTG GTCCACCCAC CAACTATGGC CCCAACCCCT 
     2941 GTCCCCAGCA GGCCTCATAT CCTGACCCCA CCCAAGAAAC ATGGGGTGAG TTCCCTTCCC 
     3001 ACTCTGGGCT GTACCCAGGC CCCAAGGCTC TAGGTGGAAC CTACAGCCAG TGTCCTCGAC 
     3061 TTGAACATTA TGGACAAGTG CAAGTCAAGC CAGAACAGGG GTGCCCAGTG GGGTCTGACT 
     3121 CCACAGGACT GGCACCCTGC CTCAATGCCC ACCCCAGTGA GGGGCCCCCA CATCCACAGC 
     3181 CTCTCTTTTC CCATTACCCC CAGCCCTCTC CTCCCCAATA TCTCCAGTCA GGCCCCTATA 
     3241 CCCAGCCACC CCCTGATTAT CTTCCTTCAG AACCCAGGCC TTGCCTGGAC TTTGATTCCC 
     3301 CCACCCATTC CACAGGGCAG CTCAAGGCTC AGCTTGTGTG TAATTATGTT CAATCTCAAC 
     3361 AGGAGCTACT GTGGGAGGGT GGGGGCAGGG AAGATGCCCC CGCCCAGGAA CCTTCCTACC 
     3421 AGAGTCCCAA GTTTCTGGGG GGTTCCCAGG TTAGCCCAAG CCGTGCTAAA GCTCCAGTGA 
     3481 ACACATATGG ACCTGGCTTT GGACCCAACT TGCCCAATCA CAAGTCAGGT TCCTATCCCA 
     3541 CCCCTTCACC ATGCCATGAA AATTTTGTAG TGGGGGCAAA TAGGGCTTCA CATAGGGCAG 
     3601 CAGCACCACC TCGACTTCTG CCCCCATTGC CCACTTGCTA TGGGCCTCTC AAAGTGGGAG 
     3661 GCACAAACCC CAGCTGTGGT CATCCTGAGG TGGGCAGGCT AGGAGGGGGT CCTGCCTTGT 
     3721 ACCCTCCTCC CGAAGGACAG GTATGTAACC CCCTGGACTC TCTTGATCTT GACAACACTC 
     3781 AGCTGGACTT TGTGGCTATT CTGGATGAGC CCCAGGGGCT GAGTCCTCCT CCTTCCCATG 
     3841 ATCAGCGGGG CAGCTCTGGA CATACCCCAC CTCCCTCTGG GCCCCCCAAC ATGGCTGTGG 
     3901 GCAACATGAG TGTCTTACTG AGATCCCTAC CTGGGGAAAC AGAATTCCTC AACTCTAGTG 
     3961 CCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4021 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4081 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4141 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4201 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4261 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4321 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4381 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4441 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4501 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4561 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4621 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4681 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4741 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4801 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4861 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4921 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4981 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5041 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5101 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5161 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5221 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5281 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5341 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5401 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5461 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5521 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5581 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5641 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5701 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5761 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5821 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5881 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5941 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6001 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6061 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6121 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6181 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6241 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6301 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6361 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6421 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6481 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6541 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6601 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6661 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6721 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6781 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6841 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6901 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6961 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7021 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7081 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7141 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7201 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7261 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7321 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7381 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7441 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7501 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7561 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7621 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7681 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7741 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7801 TTTAACGCGA ATTTT
//