LOCUS       RC229042                7686 bp    DNA     circular  SYN 
DEFINITION  SYTL2 (Myc-DDK-tagged)-Human synaptotagmin-like 2 (SYTL2), transcript variant i
ACCESSION   NM_001162953
VERSION     NM_001162953.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3843..3934 
                     /label="Myc-DDK"  
     misc_feature    3964..4549 
                     /label="PolyA signal"  
     misc_feature    4021..4040 
                     /label="Restriction Enzyme"  
     misc_feature    4825..5497 
                     /label="ColE1"  
     misc_feature    5927..6721 
                     /label="Kan/Neo"  
     misc_feature    6756..7058 
                     /label="SV40 ori"  
     misc_feature    7144..7621 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3825..3831 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3825 
                     /label="SYTL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTGACTTA AGCTTCCTGA CTGAAGAGGA ACAAGAGGCC ATCATGAAGG 
     1081 TTTTGCAGCG GGATGCTGCT CTGAAGAGGG CCGAAGAAGA GAGAGTCAGA CATTTGCCTG 
     1141 AAAAAATTAA GGATGACCAG CAGCTGAAGA ATATGAGTGG CCAATGGTTT TATGAAGCCA 
     1201 AGGCAAAAAG GCACAGGGAC AAAATCCATG GCGCAGATAT CATCAGAGCA TCTATGAGAA 
     1261 AGAAGAGGCC CCAGATAGCA GCTGAGCAGA GTAAAGACAG AGAAAATGGG GCAAAGGAAA 
     1321 GCTGGGTGAA TAATGTCAAC AAAGATGCTT TCCTTCCTCC AGAGCTGGCT GGCGTTGTAG 
     1381 AAGAGCCAGA AGAGGATGCA GCACCAGCAA GCCCGAGTTC CAGTGTGGTA AATCCAGCTT 
     1441 CCAGTGTGAT TGATATGTCC CAGGAAAACA CAAGGAAACC AAATGTGTCT CCAGAGAAGC 
     1501 AGAGGAAGAA TCCGTTTAAT AGCTCCAAGT TGCCAGAAGG TCACTCGTCA CAACAAACTA 
     1561 AAAATGAACA GTCAAAAAAT GGAAGAACTG GTTTATTTCA GACTTCAAAA GAGGATGAAT 
     1621 TGTCAGAGTC AAAAGAAAAG TCAACTGTCG CAGATACTTC AATCCAAAAG TTAGAGAAAT 
     1681 CAAAGCAGAC TTTGCCAGGC CTTTCAAATG GGTCCCAAAT CAAGGCTCCA ATCCCCAAAG 
     1741 CCAGGAAGAT GATCTACAAA TCAACTGATT TAAACAAAGA TGATAACCAG TCTTTTCCTA 
     1801 GACAAAGGAC AGACTCCCTG AAAGCGAGAG GGGCTCCGAG AGGGATCCTC AAGCGCAACT 
     1861 CCAGTTCCAG TAGCACAGAC TCAGAAACCC TTCGTTATAA TCACAACTTT GAACCCAAAA 
     1921 GCAAAATTGT GTCACCTGGC CTAACCATCC ATGAGAGAAT TTCTGAGAAG GAGCATTCTT 
     1981 TAGAAGACAA CTCTTCCCCA AACTCCCTGG AGCCATTAAA GCATGTGAGA TTCTCTGCAG 
     2041 TGAAGGATGA GCTTCCACAG AGTCCTGGGC TAATCCATGG TCGGGAAGTA GGAGAATTTA 
     2101 GTGTTTTAGA ATCTGACAGA TTGAAAAATG GAATGGAAGA TGCAGGGGAC ACAGAAGAGT 
     2161 TTCAGAGTGA CCCTAAGCCT TCTCAATACA GAAAGCCTTC GCTTTTTCAT CAATCAACCT 
     2221 CAAGCCCATA TGTATCAAAA AGTGAAACAC ATCAGCCAAT GACTTCTGGT TCTTTTCCAA 
     2281 TTAATGGGCT GCATTCTCAT TCAGAAGTTT TAACTGCAAG ACCACAGTCT ATGGAGAATT 
     2341 CACCAACCAT CAATGAACCC AAAGATAAAT CATCAGAATT AACAAGGCTT GAATCTGTAT 
     2401 TACCCAGAAG CCCTGCTGAT GAACTGTCTC ATTGTGTTGA GCCTGAGCCA TCTCAGGTGC 
     2461 CAGGTGGCAG TTCTAGAGAC CGTCAGCAAG GTTCAGAAGA AGAACCCAGT CCTGTTTTGA 
     2521 AAACTTTGGA AAGGAGTGCC GCTAGGAAAA TGCCTTCCAA AAGTCTAGAA GACATTTCAT 
     2581 CAGATTCATC AAATCAAGCA AAAGTAGATA ATCAGCCAGA AGAATTAGTG CGTAGTGCTG 
     2641 AAGATGTTTC CACAGTGCCT ACACAACCTG ATAATCCATT TTCTCACCCT GACAAACTCA 
     2701 AAAGGATGAG CAAGTCTGTT CCAGCATTTC TCCAAGATGA GAGTGATGAC AGAGAAACAG 
     2761 ATACAGCATC AGAAAGCAGT TACCAGCTCA GCAGACACAA GAAGAGCCCG AGCTCTTTAA 
     2821 CCAATCTTAG CAGCTCCTCT GGCATGACGT CCTTGTCTTC TGTGAGTGGC AGTGTGATGA 
     2881 GTGTTTATAG TGGAGACTTT GGCAATCTGG AAGTTAAAGG AAATATTCAG TTTGCAATTG 
     2941 AATATGTGGA GTCACTGAAG GAGTTGCATG TTTTTGTGGC CCAGTGTAAG GACTTAGCAG 
     3001 CAGCGGATGT AAAAAAACAG CGTTCAGACC CATATGTAAA GGCCTATTTG CTACCAGACA 
     3061 AAGGCAAAAT GGGCAAGAAG AAAACACTCG TAGTGAAGAA AACCTTGAAT CCTGTGTATA 
     3121 ACGAAATACT GCGGTATAAA ATTGAAAAAC AAATCTTAAA GACACAGAAA TTGAACCTGT 
     3181 CCATTTGGCA TCGGGATACA TTTAAGCGCA ATAGTTTCCT AGGGGAGGTG GAACTTGATT 
     3241 TGGAAACATG GGACTGGGAT AACAAACAGA ATAAACAATT GAGATGGTAC CCTCTGAAGC 
     3301 GGAAGACAGC ACCAGTTGCC CTTGAAGCAG AAAACAGAGG TGAAATGAAA CTAGCTCTCC 
     3361 AGTATGTCCC AGAGCCAGTC CCTGGTAAAA AGCTTCCTAC AACTGGAGAA GTGCACATCT 
     3421 GGGTGAAGGA ATGCCTTGAT CTACCACTGC TAAGGGGAAG TCATCTAAAT TCTTTTGTTA 
     3481 AATGTACCAT CCTTCCAGAT ACAAGTAGGA AAAGTCGCCA GAAGACAAGA GCTGTAGGGA 
     3541 AAACCACCAA CCCTATCTTC AACCACACTA TGGTGTATGA TGGGTTCAGG CCTGAAGATC 
     3601 TGATGGAAGC CTGTGTAGAG CTTACTGTCT GGGACCATTA CAAATTAACC AACCAATTTT 
     3661 TGGGAGGTCT TCGTATTGGC TTTGGAACAG GTAAAAGTTA TGGGACTGAA GTGGACTGGA 
     3721 TGGACTCTAC TTCAGAGGAA GTTGCTCTCT GGGAGAAGAT GGTAAACTCC CCCAATACTT 
     3781 GGATTGAAGC AACACTGCCT CTCAGAATGC TTTTGATTGC CAAGATTTCC AAAACGCGTA 
     3841 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3901 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3961 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4021 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4081 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4141 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4201 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4261 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4321 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4381 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4441 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4501 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4561 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4621 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4681 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4741 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4801 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4861 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4921 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4981 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5041 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5101 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5161 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5221 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5281 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5341 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5401 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5461 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5521 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5581 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5641 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5701 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5761 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5821 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5881 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5941 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6001 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6061 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6121 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6181 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6241 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6301 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6361 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6421 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6481 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6541 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6601 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6661 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6721 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6781 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6841 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6901 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6961 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7021 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7081 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7141 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7201 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7261 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7321 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7381 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7441 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7501 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7561 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7621 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7681 AATTTT
//