LOCUS       RC229040                7650 bp    DNA     circular  SYN 
DEFINITION  KCNQ5 (Myc-DDK-tagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 5 (KCNQ5), transcript variant 2
ACCESSION   NM_001160130
VERSION     NM_001160130.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3807..3898 
                     /label="Myc-DDK"  
     misc_feature    3928..4513 
                     /label="PolyA signal"  
     misc_feature    3985..4004 
                     /label="Restriction Enzyme"  
     misc_feature    4789..5461 
                     /label="ColE1"  
     misc_feature    5891..6685 
                     /label="Kan/Neo"  
     misc_feature    6720..7022 
                     /label="SV40 ori"  
     misc_feature    7108..7585 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3789..3795 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3789 
                     /label="KCNQ5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGCCAC CACGCGGGAG GAGAGGAGGG CGGCGCCGCC GGGCTCTGGG 
     1081 TGAAGAGCGG CGCAGCGGCG GCGGCGGCGG GCGGGGGGCG CTTGGGCAGC GGCATGAAGG 
     1141 ATGTGGAGTC CGGCCGGGGC AGGGTGCTGC TGAACTCGGC AGCCGCCAGG GGCGACGGCC 
     1201 TGCTACTGCT GGGCACCCGC GCGGCCACGC TCGGTGGCGG CGGCGGTGGC CTGAGGGAGA 
     1261 GCCGCCGGGG CAAGCAGGGG GCCCGGATGA GCCTGCTGGG GAAGCCGCTC TCTTACACGA 
     1321 GTAGCCAGAG CTGCCGGCGC AACGTCAAGT ACCGGCGGGT GCAGAACTAC CTGTACAACG 
     1381 TGCTGGAGAG ACCCCGCGGC TGGGCGTTCA TCTACCACGC TTTCGTTTTT CTCCTTGTCT 
     1441 TTGGTTGCTT GATTTTGTCA GTGTTTTCTA CCATCCCTGA GCACACAAAA TTGGCCTCAA 
     1501 GTTGCCTCTT GATCCTGGAG TTCGTGATGA TTGTCGTCTT TGGTTTGGAG TTCATCATTC 
     1561 GAATCTGGTC TGCGGGTTGC TGTTGTCGAT ATAGAGGATG GCAAGGAAGA CTGAGGTTTG 
     1621 CTCGAAAGCC CTTCTGTGTT ATAGATACCA TTGTTCTTAT CGCTTCAATA GCAGTTGTTT 
     1681 CTGCAAAAAC TCAGGGTAAT ATTTTTGCCA CGTCTGCACT CAGAAGTCTC CGTTTCCTAC 
     1741 AGATCCTCCG CATGGTGCGC ATGGACCGAA GGGGAGGCAC TTGGAAATTA CTGGGTTCAG 
     1801 TGGTTTATGC TCACAGCAAG GAATTAATCA CAGCTTGGTA CATAGGATTT TTGGTTCTTA 
     1861 TTTTTTCGTC TTTCCTTGTC TATCTGGTGG AAAAGGATGC CAATAAAGAG TTTTCTACAT 
     1921 ATGCAGATGC TCTCTGGTGG GGCACAATTA CATTGACAAC TATTGGCTAT GGAGACAAAA 
     1981 CTCCCCTAAC TTGGCTGGGA AGATTGCTTT CTGCAGGCTT TGCACTCCTT GGCATTTCTT 
     2041 TCTTTGCACT TCCTGCCGGC ATTCTTGGCT CAGGTTTTGC ATTAAAAGTA CAAGAACAAC 
     2101 ACCGCCAGAA ACACTTTGAG AAAAGAAGGA ACCCAGCTGC CAACCTCATT CAGTGTGTTT 
     2161 GGCGTAGTTA CGCAGCTGAT GAGAAATCTG TTTCCATTGC AACCTGGAAG CCACACTTGA 
     2221 AGGCCTTGCA CACCTGCAGC CCTACCAATC AGAAGCTAAG TTTTAAGGAG CGAGTGCGCA 
     2281 TGGCTAGCCC CAGGGGCCAG AGTATTAAGA GCCGACAAGC CTCAGTAGGT GACAGGAGGT 
     2341 CCCCAAGCAC CGACATCACA GCCGAGGGCA GTCCCACCAA AGTGCAGAAG AGCTGGAGCT 
     2401 TCAACGACCG AACCCGCTTC CGGCCCTCGC TGCGCCTCAA AAGTTCTCAG CCAAAACCAG 
     2461 TGATAGATGC TGACACAGCC CTTGGCACTG ATGATGTATA TGATGAAAAA GGATGCCAGT 
     2521 GTGATGTATC AGTGGAAGAC CTCACCCCAC CACTTAAAAC TGTCATTCGA GCTATCAGAA 
     2581 TTATGAAATT TCATGTTGCA AAACGGAAGT TTAAGGAAAC ATTACGTCCA TATGATGTAA 
     2641 AAGATGTCAT TGAACAATAT TCTGCTGGTC ATCTGGACAT GTTGTGTAGA ATTAAAAGCC 
     2701 TTCAAACACG TGTTGATCAA ATTCTTGGAA AAGGGCAAAT CACATCAGAT AAGAAGAGCC 
     2761 GAGAGAAAAT AACAGCAGAA CATGAGACCA CAGACGATCT CAGTATGCTC GGTCGGGTGG 
     2821 TCAAGGTTGA AAAACAGGTA CAGTCCATAG AATCCAAGCT GGACTGCCTA CTAGACATCT 
     2881 ATCAACAGGT CCTTCGGAAA GGCTCTGCCT CAGCCCTCGC TTTGGCTTCA TTCCAGATCC 
     2941 CACCTTTTGA ATGTGAACAG ACATCTGACT ATCAAAGCCC TGTGGATAGC AAAGATCTTT 
     3001 CGGGTTCCGC ACAAAACAGT GGCTGCTTAT CCAGATCAAC TAGTGCCAAC ATCTCGAGAG 
     3061 GCCTGCAGTT CATTCTGACG CCAAATGAGT TCAGTGCCCA GACTTTCTAC GCGCTTAGCC 
     3121 CTACTATGCA CAGTCAAGCA ACACAGGTGC CAATTAGTCA AAGCGATGGC TCAGCAGTGG 
     3181 CAGCCACCAA CACCATTGCA AACCAAATAA ATACGGCACC CAAGCCAGCA GCCCCAACAA 
     3241 CTTTACAGAT CCCACCTCCT CTCCCAGCCA TCAAGCATCT GCCCAGGCCA GAAACTCTGC 
     3301 ACCCTAACCC TGCAGGCTTA CAGGAAAGCA TTTCTGACGT CACCACCTGC CTTGTTGCCT 
     3361 CCAAGGAAAA TGTTCAGGTT GCACAGTCAA ATCTCACCAA GGACCGTTCT ATGAGGAAAA 
     3421 GCTTTGACAT GGGAGGAGAA ACTCTGTTGT CTGTCTGTCC CATGGTGCCG AAGGACTTGG 
     3481 GCAAATCTTT GTCTGTGCAA AACCTGATCA GGTCGACCGA GGAACTGAAT ATACAACTTT 
     3541 CAGGGAGTGA GTCAAGTGGC TCCAGAGGCA GCCAAGATTT TTACCCCAAA TGGAGGGAAT 
     3601 CCAAATTGTT TATAACTGAT GAAGAGGTGG GTCCCGAAGA GACAGAGACA GACACTTTTG 
     3661 ATGCCGCACC GCAGCCTGCC AGGGAAGCTG CCTTTGCATC AGACTCTCTA AGGACTGGAA 
     3721 GGTCACGATC ATCTCAGAGC ATTTGTAAGG CAGGAGAAAG TACAGATGCC CTCAGCTTGC 
     3781 CTCATGTCAA ACTGAAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3841 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3901 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3961 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4021 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4081 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4141 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4201 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4261 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4321 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4381 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4441 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4501 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4561 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4621 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4681 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4741 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4801 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4861 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4921 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4981 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5041 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5101 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5161 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5221 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5281 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5341 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5401 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5461 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5521 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5581 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5641 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5701 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5761 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5821 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5881 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5941 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6001 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6061 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6121 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6181 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6241 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6301 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6361 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6421 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6481 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6541 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6601 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6661 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6721 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6781 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6841 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6901 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6961 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7021 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7081 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7141 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7201 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7261 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7321 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7381 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7441 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7501 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7561 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7621 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//