LOCUS       RC228998                6966 bp    DNA     circular  SYN 
DEFINITION  NRG3 (Myc-DDK-tagged)-Human neuregulin 3 (NRG3), transcript variant 2
ACCESSION   NM_001165972
VERSION     NM_001165972.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3123..3214 
                     /label="Myc-DDK"  
     misc_feature    3244..3829 
                     /label="PolyA signal"  
     misc_feature    3301..3320 
                     /label="Restriction Enzyme"  
     misc_feature    4105..4777 
                     /label="ColE1"  
     misc_feature    5207..6001 
                     /label="Kan/Neo"  
     misc_feature    6036..6338 
                     /label="SV40 ori"  
     misc_feature    6424..6901 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3105..3111 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3105 
                     /label="NRG3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGAAGGG GCGGCCGCTG CCTCGCCACC TGGTGCCGCT TCGGCAGCCG 
     1081 CCGCCTCGGC CGAGGAGGGC ACCGCGGCGG CTGCGGCGGC GGCAGCGGCG GGCGGGGGCC 
     1141 CGGACGGCGG CGGCGAAGGG GCGGCCGAGC CCCCCCGGGA GTTACGCTGT AGCGACTGCA 
     1201 TCGTGTGGAA CCGGCAGCAG ACGTGGCTGT GCGTGGTACC TCTGTTCATC GGCTTCATCG 
     1261 GCCTGGGGCT CAGCCTCATG CTTCTCAAAT GGATCGTGGT GGGCTCCGTC AAGGAGTACG 
     1321 TGCCCACCGA CCTAGTGGAC TCCAAGGGGA TGGGCCAGGA CCCCTTCTTC CTCTCCAAGC 
     1381 CCAGCTCTTT CCCCAAGGCC ATGGAGACCA CCACCACTAC CACTTCCACC ACGTCCCCCG 
     1441 CCACCCCCTC CGCCGGGGGT GCCGCCTCCT CCAGGACGCC CAACCGGATT AGCACTCGCC 
     1501 TGACCACCAT CACGCGGGCG CCCACTCGCT TCCCCGGGCA CCGGGTGCCC ATCCGGGCCA 
     1561 GCCCGCGCTC CACCACAGCA CGGAACACTG CGGCCCCTGC GACGGTCCCG TCCACCACGG 
     1621 CCCCGTTCTT CAGTAGCAGC ACGCTGGGCT CCCGACCCCC GGTGCCAGGA ACTCCAAGTA 
     1681 CCCAGGCAAT GCCCTCCTGG CCTACTGCGG CATACGCTAC CTCCTCCTAC CTTCACGATT 
     1741 CTACTCCCTC CTGGACCCTG TCTCCCTTTC AGGATGCTGC CTCCTCTTCT TCCTCTTCTT 
     1801 CCTCCTCCGC TACCACCACC ACACCAGAAA CTAGCACCAG CCCCAAATTT CATACGACGA 
     1861 CATATTCCAC AGAGCGATCC GAGCACTTCA AACCCTGCCG AGACAAGGAC CTTGCATACT 
     1921 GTCTCAATGA TGGCGAGTGC TTTGTGATCG AAACCCTGAC CGGATCCCAT AAACACTGTC 
     1981 GGTGCAAAGA AGGCTACCAA GGAGTCCGTT GTGATCAATT TCTGCCGAAA ACTGATTCCA 
     2041 TCTTATCGGA TCCAAACCAC TTGGGGATTG AATTCATGGA GAGTGAAGAA GTTTATCAAA 
     2101 GGCAGGTGCT GTCAATTTCA TGTATCATCT TTGGAATTGT CATCGTGGGC ATGTTCTGTG 
     2161 CAGCATTCTA CTTCAAAAGC AAGAAACAAG CTAAACAAAT CCAAGAGCAG CTGAAAGTGC 
     2221 CACAAAATGG TAAAAGCTAC AGTCTCAAAG CATCCAGCAC AATGGCAAAG TCAGAGAACT 
     2281 TGGTGAAGAG CCATGTCCAG CTGCAAAATT ATTCAAAGGT GGAAAGGCAT CCTGTGACTG 
     2341 CATTGGAGAA AATGATGGAG TCAAGTTTTG TCGGCCCCCA GTCATTCCCT GAGGTCCCTT 
     2401 CTCCTGACAG AGGAAGCCAG TCTGTCAAAC ACCACAGGAG TCTATCCTCT TGCTGCAGCC 
     2461 CAGGGCAAAG AAGTGGCATG CTCCATAGGA ATGCCTTCAG AAGGACACCC CCGTCACCCC 
     2521 GAAGTAGGCT AGGTGGAATT GTGGGACCAG CATATCAGCA ACTCGAAGAA TCAAGGATCC 
     2581 CAGACCAGGA TACGATACCT TGCCAAGGGT ATTCATCCAG TGGTTTAAAA ACCCAACGAA 
     2641 ATACATCAAT AAATATGCAA CTGCCTTCAA GAGAGACAAA CCCCTATTTT AATAGCTTGG 
     2701 AGCAAAAGGA CCTGGTGGGC TATTCATCCA CAAGGGCCAG TTCTGTGCCC ATCATCCCTT 
     2761 CAGTGGGTTT AGAGGAAACC TGCCTGCAAA TGCCAGGGAT TTCTGAAGTC AAAAGCATCA 
     2821 AATGGTGCAA AAACTCCTAT TCAGCTGACG TTGTCAATGT GAGTATTCCA GTCAGCGATT 
     2881 GTCTTATAGC AGAACAACAA GAAGTGAAAA TATTGCTAGA AACTGTCCAG GAGCAGATCC 
     2941 GAATTCTGAC TGATGCCAGA CGGTCAGAAG ACTACGAACT GGCCAGCGTA GAAACCGAGG 
     3001 ACAGTGCAAG CGAAAACACA GCCTTTCTCC CCCTGAGTCC CACAGCCAAA TCAGAACGAG 
     3061 AGGCGCAATT TGTCTTAAGA AATGAAATAC AAAGAGACTC TGCATTGACC AAGACGCGTA 
     3121 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3181 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3241 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3301 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3361 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3421 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3481 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3541 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3601 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3661 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3721 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3781 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     3841 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     3901 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     3961 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4021 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4081 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4141 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4201 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4261 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4321 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4381 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4441 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4501 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4561 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4621 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4681 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4741 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4801 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     4861 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     4921 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     4981 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5041 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5101 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5161 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5221 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5281 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5341 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5401 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5461 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5521 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5581 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5641 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5701 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5761 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     5821 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     5881 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     5941 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6001 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6061 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6121 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6181 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6241 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6301 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6361 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6421 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6481 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6541 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6601 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6661 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6721 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6781 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     6841 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     6901 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     6961 AATTTT
//