LOCUS       RC228978                6765 bp    DNA     circular  SYN 
DEFINITION  JPH4 (Myc-DDK-tagged)-Human junctophilin 4 (JPH4), transcript variant 2
ACCESSION   NM_001146028
VERSION     NM_001146028.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2922..3013 
                     /label="Myc-DDK"  
     misc_feature    3043..3628 
                     /label="PolyA signal"  
     misc_feature    3100..3119 
                     /label="Restriction Enzyme"  
     misc_feature    3904..4576 
                     /label="ColE1"  
     misc_feature    5006..5800 
                     /label="Kan/Neo"  
     misc_feature    5835..6137 
                     /label="SV40 ori"  
     misc_feature    6223..6700 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2904..2910 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2904 
                     /label="JPH4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCCCGGG GGCAAGTTCG ACTTTGACGA CGGGGGCTGC TACGTGGGGG 
     1081 GCTGGGAGGC GGGGCGGGCA CATGGCTACG GCGTGTGCAC GGGCCCCGGC GCCCAGGGCG 
     1141 AGTACAGCGG CTGCTGGGCA CACGGCTTCG AGTCACTGGG CGTCTTCACG GGGCCCGGCG 
     1201 GACACAGCTA CCAGGGCCAC TGGCAGCAGG GCAAGCGCGA AGGGCTGGGC GTGGAGCGCA 
     1261 AGAGCCGCTG GACGTACCGC GGCGAGTGGC TGGGCGGGCT GAAGGGGCGC AGCGGCGTGT 
     1321 GGGAAAGCGT GTCCGGCCTG CGCTACGCCG GGCTCTGGAA GGACGGTTTC CAGGACGGCT 
     1381 ACGGCACTGA GACCTACTCC GACGGAGGCA CCTACCAGGG CCAGTGGCAG GCCGGGAAGC 
     1441 GCCACGGCTA CGGGGTACGC CAGAGTGTGC CCTACCATCA GGCGGCGCTG CTGCGCTCGC 
     1501 CCCGCCGCAC CTCCCTGGAT TCCGGCCACA GCGACCCCCC GACGCCACCC CCGCCCCTGC 
     1561 CCTTGCCGGG CGACGAGGGA GGCAGCCCCG CCTCGGGCTC CCGGGGCGGC TTCGTGCTGG 
     1621 CCGGGCCCGG GGACGCCGAC GGCGCGTCGT CCCGAAAGCG CACTCCAGCG GCCGGCGGAT 
     1681 TCTTTCGCCG TTCGCTGCTG CTCAGCGGGC TCCGAGCGGG CGGACGTCGC AGCTCCCTGG 
     1741 GCAGCAAGCG AGGCTCCCTG CGCAGCGAGG TGAGCAGCGA GGTGGGCAGC ACCGGACCGC 
     1801 CCGGCTCGGA GGCCAGCGGG CCCCCGGCCG CAGCGCCGCC CGCCCTCATC GAGGGCTCGG 
     1861 CCACAGAGGT GTACGCGGGC GAGTGGCGCG CAGATCGGCG CAGCGGCTTC GGCGTCAGCC 
     1921 AGCGCTCCAA CGGGCTGCGC TACGAGGGCG AGTGGCTGGG CAACCAGCGG CACGGCTACG 
     1981 GGCGCACCAC CCGCCCCGAC GGCTCCCGCG AGGAGGGCAA GTACAAGCGC AACCGGCTGG 
     2041 TGCACGGCGG GCGCGTCCGC AGTCTCCTGC CTCTGGCCCT TCGGCGGGGC AAGGTTAAGG 
     2101 AGAAGGTGGA CAGGGCTGTC GAGGGCGCCC GTCGAGCCGT GAGTGCTGCC CGTCAGCGCC 
     2161 AGGAGATCGC CGCTGCCAGG GCAGCAGACG CCCTCCTAAA GGCAGTGGCA GCCAGCAGTG 
     2221 TCGCTGAGAA GGCCGTGGAG GCAGCTCGAA TGGCCAAACT GATAGCCCAG GACCTGCAGC 
     2281 CCATGCTAGA GGCCCCAGGC CGCAGACCCA GGCAGGACTC AGAAGGTTCC GACACGGAGC 
     2341 CCCTGGATGA GGACAGCCCT GGGGTATATG AGAACGGACT GACCCCCTCA GAGGGATCCC 
     2401 CTGAACTGCC CAGCAGTCCT GCCTCCTCCC GCCAACCCTG GCGACCCCCT GCCTGCCGGA 
     2461 GCCCACTGCC TCCTGGAGGG GACCAGGGTC CCTTCTCCAG CCCCAAAGCT TGGCCTGAGG 
     2521 AGTGGGGGGG GGCAGGCGCA CAGGCAGAGG AACTAGCTGG CTATGAGGCT GAGGATGAGG 
     2581 CTGGGATGCA AGGGCCAGGG CCCAGAGACG GTTCCCCACT CCTCGGAGGC TGCAGCGACA 
     2641 GTTCAGGAAG TCTTCGAGAG GAGGAGGGGG AGGATGAAGA GCCCCTGCCC CCGCTGAGGG 
     2701 CCCCAGCAGG CACGGAGCCT GAGCCCATCG CCATGCTGGT CCTGAGGGGC TCGTCCTCGA 
     2761 GGGGTCATGA TGCTGGGTGC CTGACAGAAG AGCTCGGGGA GCCCGCTGCA ACCGAGAGGC 
     2821 CTGCCCAGCC GGGAGCTGCC AACCCCCTGG TGGTGGGAGC CGTGGCCCTC CTGGACCTCA 
     2881 GCCTGGCATT CCTGTTCTCC CAGCTCCTCA CCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     2941 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3001 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3061 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3121 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3181 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3241 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3301 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3361 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3421 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3481 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3541 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3601 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3661 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     3721 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     3781 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     3841 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     3901 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     3961 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4021 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4081 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4141 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4201 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4261 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4321 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4381 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4441 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4501 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4561 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4621 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4681 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     4741 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     4801 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     4861 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     4921 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     4981 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5041 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5101 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5161 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5221 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5281 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5341 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5401 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5461 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5521 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5581 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5641 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     5701 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     5761 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     5821 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     5881 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     5941 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6001 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6061 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6121 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6181 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6241 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6301 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6361 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6421 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6481 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6541 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6601 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6661 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     6721 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//