LOCUS       RC228682                9408 bp    DNA     circular  SYN 
DEFINITION  RAPGEF6 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant 3
ACCESSION   NM_001164387
VERSION     NM_001164387.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5565..5656 
                     /label="Myc-DDK"  
     misc_feature    5686..6271 
                     /label="PolyA signal"  
     misc_feature    5743..5762 
                     /label="Restriction Enzyme"  
     misc_feature    6547..7219 
                     /label="ColE1"  
     misc_feature    7649..8443 
                     /label="Kan/Neo"  
     misc_feature    8478..8780 
                     /label="SV40 ori"  
     misc_feature    8866..9343 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5547..5553 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5547 
                     /label="RAPGEF6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTCACCC GTGGACCCTG GCGCTAGGCA GGCGTTGAGG AAGAAGCCAC 
     1081 CCGAGCGGAC TCCCGAGGAC TTAAATACTA TTTATTCTTA TCTTCATGGA ATGGAAATAT 
     1141 TATCAAATCT CAGGGAACAT CAGCTTAGAT TAATGTCTGC AAGAGCACGC TATGAGAGAT 
     1201 ACAGTGGCAA TCAGGTTCTC TTTTGTTCAG AAACGATTGC CAGATGTTGG TATATCCTAC 
     1261 TTTCTGGATC TGTGCTTGTG AAAGGCTCCA TGGTCTTGCC TCCTTGCAGT TTTGGTAAGC 
     1321 AGTTTGGAGG AAAAAGAGGA TGTGATTGTC TTGTATTAGA GCCTTCAGAA ATGATTGTGG 
     1381 TAGAGAATGC CAAAGATAAT GAAGATAGTA TTCTACAAAG AGAAATTCCT GCCAGACAAT 
     1441 CCCGAAGAAG ATTTCGGAAA ATTAACTATA AAGGAGAGCG CCAAACCATT ACTGATGATG 
     1501 TGGAGGTTAA CAGCTATCTT TCTCTTCCAG CTGATCTTAC CAAGATGCAT CTCACAGAAA 
     1561 ACCCTCATCC ACAGGTGACT CATGTGTCTT CTAGTCAGTC TGGTTGTAGC ATTGCCAGTG 
     1621 ACTCTGGAAG CAGCAGTTTA TCTGATATCT ATCAGGCTAC GGAGAGTGAG GTAGGAGATG 
     1681 TAGATTTGAC ACGTCTTCCA GAAGGACCTG TTGATTCTGA GGATGACGAA GAGGAAGATG 
     1741 AAGAGATTGA TCGAACAGAT CCATTGCAGG GGCGAGATCT TGTTCGAGAA TGTCTTGAAA 
     1801 AAGAACCTGC AGACAAAACT GATGATGACA TTGAACAATT GCTGGAGTTT ATGCACCAGC 
     1861 TCCCTGCATT TGCAAACATG ACCATGTCTG TAAGGAGAGA ACTCTGCTCA GTGATGATTT 
     1921 TTGAAGTGGT AGAGCAGGCT GGAGCTATTA TTCTTGAAGA TGGGCAAGAG CTTGACTCAT 
     1981 GGTATGTTAT TTTAAACGGC ACTGTGGAAA TCAGTCATCC AGATGGAAAA GTTGAAAATT 
     2041 TGTTTATGGG AAATAGTTTT GGAATTACTC CCACTCTGGA TAAGCAGTAC ATGCATGGAA 
     2101 TTGTCAGGAC TAAAGTAGAT GATTGTCAGT TTGTCTGCAT AGCCCAGCAA GATTATTGGA 
     2161 GAATTTTAAA CCATGTGGAA AAAAATACCC ATAAAGTTGA GGAAGAGGGA GAAATTGTTA 
     2221 TGGTACATGA GCATCGGGAA CTAGACCGGA GTGGAACCAG GAAAGGACAC ATTGTGATCA 
     2281 AGGCAACACC TGAGCGTCTC ATAATGCATT TAATAGAAGA ACATTCCATC GTGGATCCAA 
     2341 CTTATATAGA AGATTTTCTA TTAACTTACA GGACATTTCT TGAAAGTCCT TTGGATGTTG 
     2401 GGATCAAACT ATTGGAATGG TTTAAGATCG ACAGCTTAAG AGATAAGGTG ACACGGATTG 
     2461 TATTATTATG GGTAAATAAT CATTTTAATG ATTTTGAAGG TGACCCTGCT ATGACTCGAT 
     2521 TTCTAGAGGA ATTTGAAAAA AATCTGGAAG ATACAAAGAT GAATGGTCAT CTCCGGTTAT 
     2581 TGAATATTGC CTGTGCTGCA AAGGCTAAGT GGAGACAGGT TGTGCTGCAA AAGGCTTCCC 
     2641 GCGAGTCCCC TCTACAATTC AGCCTTAATG GAGGGAGTGA GAAGGGATTT GGTATTTTTG 
     2701 TTGAAGGAGT AGAACCTGGT AGCAAAGCTG CTGATTCAGG ACTGAAACGT GGTGATCAGA 
     2761 TTATGGAAGT AAATGGACAA AACTTTGAGA ATATTACATT TATGAAAGCC GTTGAAATTT 
     2821 TGAGGAATAA TACTCATCTT GCACTTACTG TGAAGACCAA CATTTTTGTG TTCAAAGAGT 
     2881 TACTTTTTAG GACTGAACAA GAGAAATCTG GTGTTCCTCA TATTCCCAAA ATTGCTGAAA 
     2941 AAAAAAGTAA TCGCCATTCT ATCCAGCATG TGCCAGGAGA TATTGAACAG ACATCACAGG 
     3001 AGAAAGGAAG TAAGAAAGTT AAAGCAAATA CTGTTTCAGG TGGAAGAAAC AAAATCAGGA 
     3061 AGATTTTGGA TAAAACACGA TTTAGTATCT TGCCTCCAAA GCTATTTAGT GATGGAGGCC 
     3121 TAAGCCAATC ACAAGATGAC AGCATTGTGG GAACAAGGCA CTGTAGGCAT AGTCTGGCTA 
     3181 TAATGCCCAT CCCTGGAACA CTCTCATCCA GCAGCCCTGA TCTCCTGCAG CCTACCACCA 
     3241 GTATGTTGGA TTTTTCCAAT CCTTCAGCTG TTGGTTTTTA TTATATCCCT GATCAAGTTA 
     3301 TAAGAGTTTT CAAAGTGGAT CAGCAAAGTT GCTACATTAT CATCAGTAAA GACACCACAG 
     3361 CTAAAGAAGT AGTTTTTCAT GCTGTTCATG AATTTGGTTT GACCGGTGCA TCCGACACAT 
     3421 ATTCTCTCTG TGAAGTTTCT GTTACTCCTG AGGGTGTCAT AAAACAGAGA AGACTTCCAG 
     3481 ATCAGTTCTC CAAATTAGCT GATAGAATTC AACTCAATGG AAGGTATTAC TTAAAAAATA 
     3541 ACATGGAAAC AGAAACCTTA TGTTCAGATG AAGATGCTCA AGAACTAGTT AAGGAAAGCC 
     3601 AGCTATCCAT GCTGCAGCTC AGTACCATTG AGGTGGCCAC CCAGCTGTCA ATGAGGGACT 
     3661 TTGATTTGTT TCGTAATATT GAACCGACTG AGTACATCGA TGACCTTTTT AAGTTAAATT 
     3721 CCAAAACAGG AAATACTCAT TTGAAGAGGT TTGAGGACAT TGTAAACCAA GAGACATTCT 
     3781 GGGTTGCCTC AGAAATTTTA ACTGAAGCAA ATCAGCTCAA ACGAATGAAG ATTATTAAGC 
     3841 ATTTTATTAA AATTGCACTT CATTGTCGAG AATGTAAGAA CTTCAATTCC ATGTTTGCAA 
     3901 TAATAAGTGG CTTGAACCTG GCATCTGTAG CAAGACTCAG AGGAACTTGG GAAAAGTTAC 
     3961 CAAGCAAATA CGAGAAACAT CTTCAAGATC TACAAGACAT TTTTGATCCA TCTAGAAACA 
     4021 TGGCAAAGTA TAGAAATATT CTTAGTAGTC AAAGTATGCA GCCTCCAATT ATTCCACTCT 
     4081 TCCCTGTTGT CAAGAAAGAT ATGACATTTC TACATGAAGG AAATGACTCC AAAGTAGATG 
     4141 GTTTAGTAAA CTTTGAGAAG TTAAGAATGA TTTCCAAGGA AATCCGCCAA GTTGTTCGAA 
     4201 TGACTTCTGC TAACATGGAC CCAGCTATGA TGTTTCGACA GAGGAAGAAG AGGTGGCGGA 
     4261 GTCTGGGGTC ACTGAGTCAA GGAAGCACAA ATTCAAACAT GCTGGATGTT CAGGGAGGTG 
     4321 CTCACAAAAA AAGGGCACGC CGCAGCTCTC TGCTTAATGC CAAGAAGCTA TATGAGGATG 
     4381 CCCAAATGGC AAGGAAGGTG AAGCAGTATC TTTCCAGTCT CGATGTAGAG ACAGATGAGG 
     4441 AGAAGTTCCA GATGATGTCA TTACAGTGGG AGCCTGCATA TGGTACCTTG ACCAAGAATT 
     4501 TAAGTGAGAA AAGATCAGCC AAATCATCTG AAATGTCTCC AGTGCCTATG AGGTCAGCTG 
     4561 GCCAAACAAC TAAAGCCCAC TTGCATCAAC CCCACAGAGT AAGCCAGGTG CTTCAGGTGC 
     4621 CAGCTGTTAA TTTGCACCCC ATCAGGAAGA AGGGACAAAC AAAAGACCCT GCACTGAATA 
     4681 CAAGTTTACC TCAGAAAGTT TTAGGAACAA CTGAAGAAAT AAGTGGTAAG AAGCATACAG 
     4741 AAGACACTAT TTCTGTGGCG TCATCTTTAC ATTCTAGTCC TCCTGCATCT CCTCAAGGCT 
     4801 CCCCTCACAA AGGTTACACA CTTATTCCAT CAGCTAAATC TGACAACTTG TCTGACTCCA 
     4861 GCCATAGTGA GATTTCTTCA CGGTCCAGCA TCGTGAGCAA TTGTTCTGTT GACTCCATGT 
     4921 CTGCAGCTCT ACAGGATGAA CGGTGTTCCT CTCAGGCCCT GGCAGTCCCT GAATCCACTG 
     4981 GGGCATTGGA AAAGACAGAG CACGCTTCAG GGATAGGAGA TCATAGTCAA CATGGCCCTG 
     5041 GGTGGACACT CTTGAAGCCA TCTCTAATCA AGTGTTTAGC TGTCTCATCG TCTGTGAGCA 
     5101 ATGAAGAGAT TTCTCAAGAG CATATCATTA TAGAAGCAGC TGACAGTGGT CGTGGAAGTT 
     5161 GGACTTCGTG TTCAAGCAGC TCCCATGACA ACTTCCAAAG CCTTCCAAAC CCAAAAAGCT 
     5221 GGGATTTTTT GAACTCTTAC AGACATACCC ATTTGGATGA CCCCATTGCT GAAGTTGAAC 
     5281 CCACTGACTC TGAGCCCTAT TCCTGTTCTA AAAGCTGCTC TAGAACTTGT GGGCAGTGTA 
     5341 AAGGAAGCCT AGAGAGAAAG AGTTGGACCT CCTCCAGTTC TCTGTCTGAC ACGTATGAAC 
     5401 CAAACTATGG GACAGTTAAA CAGAGAGTAT TGGAGAGCAC CCCAGCTGAG TCATCTGAAG 
     5461 GCTTGGACCC CAAGGATGCC ACTGACCCAG TTTATAAAAC TGTCACTTCA AGTACAGAAA 
     5521 AGGGCTTGAT TGAAAATGAA CAAGTTTCAG CAGTCACGCG TACGCGGCCG CTCGAGCAGA 
     5581 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5641 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5701 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5761 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5821 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5881 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5941 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     6001 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     6061 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     6121 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     6181 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     6241 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     6301 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     6361 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6421 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6481 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6541 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6601 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6661 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6721 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6781 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6841 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6901 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6961 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     7021 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     7081 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     7141 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     7201 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     7261 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     7321 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     7381 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7441 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7501 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7561 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7621 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7681 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7741 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7801 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7861 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7921 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7981 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     8041 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     8101 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     8161 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     8221 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     8281 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     8341 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     8401 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8461 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8521 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8581 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8641 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8701 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8761 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8821 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8881 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8941 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     9001 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     9061 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     9121 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     9181 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     9241 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     9301 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     9361 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//