LOCUS       RC228681                9165 bp    DNA     circular  SYN 
DEFINITION  CD109 (Myc-DDK-tagged)-Human CD109 molecule (CD109), transcript variant 2
ACCESSION   NM_001159587
VERSION     NM_001159587.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5322..5413 
                     /label="Myc-DDK"  
     misc_feature    5443..6028 
                     /label="PolyA signal"  
     misc_feature    5500..5519 
                     /label="Restriction Enzyme"  
     misc_feature    6304..6976 
                     /label="ColE1"  
     misc_feature    7406..8200 
                     /label="Kan/Neo"  
     misc_feature    8235..8537 
                     /label="SV40 ori"  
     misc_feature    8623..9100 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5304..5310 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5304 
                     /label="CD109"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGGCCCA CCGCTCCTGA CCGCCGCCCA CCTCCTCTGC GTGTGCACCG 
     1081 CCGCGCTGGC CGTGGCTCCC GGGCCTCGGT TTCTGGTGAC AGCCCCAGGG ATCATCAGGC 
     1141 CCGGAGGAAA TGTGACTATT GGGGTGGAGC TTCTGGAACA CTGCCCTTCA CAGGTGACTG 
     1201 TGAAGGCGGA GCTGCTCAAG ACAGCATCAA ACCTCACTGT CTCTGTCCTG GAAGCAGAAG 
     1261 GAGTCTTTGA AAAAGGCTCT TTTAAGACAC TTACTCTTCC ATCACTACCT CTGAACAGTG 
     1321 CAGATGAGAT TTATGAGCTA CGTGTAACCG GACGTACCCA GGATGAGATT TTATTCTCTA 
     1381 ATAGTACCCG CTTATCATTT GAGACCAAGA GAATATCTGT CTTCATTCAA ACAGACAAGG 
     1441 CCTTATACAA GCCAAAGCAA GAAGTGAAGT TTCGCATTGT TACACTCTTC TCAGATTTTA 
     1501 AGCCTTACAA AACCTCTTTA AACATTCTCA TTAAGGACCC CAAATCAAAT TTGATCCAAC 
     1561 AGTGGTTGTC ACAACAAAGT GATCTTGGAG TCATTTCCAA AACTTTTCAG CTATCTTCCC 
     1621 ATCCAATACT TGGTGACTGG TCTATTCAAG TTCAAGTGAA TGACCAGACA TATTATCAAT 
     1681 CATTTCAGGT TTCAGAATAT GTATTACCAA AATTTGAAGT GACTTTGCAG ACACCATTAT 
     1741 ATTGTTCTAT GAATTCTAAG CATTTAAATG GTACCATCAC GGCAAAGTAT ACATATGGGA 
     1801 AGCCAGTGAA AGGAGACGTA ACGCTTACAT TTTTACCTTT ATCCTTTTGG GGAAAGAAGA 
     1861 AAAATATTAC AAAAACATTT AAGATAAATG GATCTGCAAA CTTCTCTTTT AATGATGAAG 
     1921 AGATGAAAAA TGTAATGGAT TCTTCAAATG GACTTTCTGA ATACCTGGAT CTATCTTCCC 
     1981 CTGGACCAGT AGAAATTTTA ACCACAGTGA CAGAATCAGT TACAGGTATT TCAAGAAATG 
     2041 TAAGCACTAA TGTGTTCTTC AAGCAACATG ATTACATCAT TGAGTTTTTT GATTATACTA 
     2101 CTGTCTTGAA GCCATCTCTC AACTTCACAG CCACTGTGAA GGTAACTCGT GCTGATGGCA 
     2161 ACCAACTGAC TCTTGAAGAA AGAAGAAATA ATGTAGTCAT AACAGTGACA CAGAGAAACT 
     2221 ATACTGAGTA CTGGAGCGGA TCTAACAGTG GAAATCAGAA AATGGAAGCT GTTCAGAAAA 
     2281 TAAATTATAC TGTCCCCCAA AGTGGAACTT TTAAGATTGA ATTCCCAATC CTGGAGGATT 
     2341 CCAGTGAGCT ACAGTTGAAG GCCTATTTCC TTGGTAGTAA AAGTAGCATG GCAGTTCATA 
     2401 GTCTGTTTAA GTCTCCTAGT AAGACATACA TCCAACTAAA AACAAGAGAT GAAAATATAA 
     2461 AGGTGGGATC GCCTTTTGAG TTGGTGGTTA GTGGCAACAA ACGATTGAAG GAGTTAAGCT 
     2521 ATATGGTAGT ATCCAGGGGA CAGTTGGTGG CTGTAGGAAA ACAAAATTCA ACAATGTTCT 
     2581 CTTTAACACC AGAAAATTCT TGGACTCCAA AAGCCTGTGT AATTGTGTAT TATATTGAAG 
     2641 ATGATGGGGA AATTATAAGT GATGTTCTAA AAATTCCTGT TCAGCTTGTT TTTAAAAATA 
     2701 AGATAAAGCT ATATTGGAGT AAAGTGAAAG CTGAACCATC TGAGAAAGTC TCTCTTAGGA 
     2761 TCTCTGTGAC ACAGCCTGAC TCCATAGTTG GGATTGTAGC TGTTGACAAA AGTGTGAATC 
     2821 TGATGAATGC CTCTAATGAT ATTACAATGG AAAATGTGGT CCATGAGTTG GAACTTTATA 
     2881 ACACAGGATA TTATTTAGGC ATGTTCATGA ATTCTTTTGC AGTCTTTCAG GAATGTGGAC 
     2941 TCTGGGTATT GACAGATGCA AACCTCACGA AGGATTATAT TGATGGTGTT TATGACAATG 
     3001 CAGAATATGC TGAGAGGTTT ATGGAGGAAA ATGAAGGACA TATTGTAGAT ATTCATGACT 
     3061 TTTCTTTGGG TAGCAGTCCA CATGTCCGAA AGCATTTTCC AGAGACTTGG ATTTGGCTAG 
     3121 ACACCAACAT GGGTTACAGG ATTTACCAAG AATTTGAAGT AACTGTACCT GATTCTATCA 
     3181 CTTCTTGGGT GGCTACTGGT TTTGTGATCT CTGAGGACCT GGGTCTTGGA CTAACAACTA 
     3241 CTCCAGTGGA GCTCCAAGCC TTCCAACCAT TTTTCATTTT TTTGAATCTT CCCTACTCTG 
     3301 TTATCAGAGG TGAAGAATTT GCTTTGGAAA TAACTATATT CAATTATTTG AAAGATGCCA 
     3361 CTGAGGTTAA GGTAATCATT GAGAAAAGTG ACAAATTTGA TATTCTAATG ACTTCAAATG 
     3421 AAATAAATGC CACAGGCCAC CAGCAGACCC TTCTGGTTCC CAGTGAGGAT GGGGCAACTG 
     3481 TTCTTTTTCC CATCAGGCCA ACACATCTGG GAGAAATTCC TATCACAGTC ACAGCTCTTT 
     3541 CACCCACTGC TTCTGATGCT GTCACCCAGA TGATTTTAGT AAAGGCTGAA GGAATAGAAA 
     3601 AATCATATTC ACAATCCATC TTATTAGACT TGACTGACAA TAGGCTACAG AGTACCCTGA 
     3661 AAACTTTGAG TTTCTCATTT CCTCCTAATA CAGTGACTGG CAGTGAAAGA GTTCAGATCA 
     3721 CTGCAATTGG AGATGTTCTT GGTCCTTCCA TCAATGGCTT AGCCTCATTG ATTCGGATGC 
     3781 CTTATGGCTG TGGTGAACAG AACATGATAA ATTTTGCTCC AAATATTTAC ATTTTGGATT 
     3841 ATCTGACTAA AAAGAAACAA CTGACAGATA ATTTGAAAGA AAAAGCTCTT TCATTTATGA 
     3901 GGCAAGGTTA CCAGAGAGAA CTTCTCTATC AGAGGGAAGA TGGCTCTTTC AGTGCTTTTG 
     3961 GGAATTATGA CCCTTCTGGG AGCACTTGGT TGTCAGCTTT TGTTTTAAGA TGTTTCCTTG 
     4021 AAGCCGATCC TTACATAGAT ATTGATCAGA ATGTGTTACA CAGAACATAC ACTTGGCTTA 
     4081 AAGGACATCA GAAATCCAAC GGTGAATTTT GGGATCCAGG AAGAGTGATT CATAGTGAGC 
     4141 TTCAAGGTGG CAATAAAAGT CCAGTAACAC TTACAGCCTA TATTGTAACT TCTCTCCTGG 
     4201 GATATAGAAA GTATCAGCCT AACATTGATG TGCAAGAGTC TATCCATTTT TTGGAGTCTG 
     4261 AATTCAGTAG AGGAATTTCA GACAATTATA CTCTAGCCCT TATAACTTAT GCATTGTCAT 
     4321 CAGTGGGGAG TCCTAAAGCG AAGGAAGCTT TGAATATGCT GACTTGGAGA GCAGAACAAG 
     4381 AAGGTGGCAT GCAATTCTGG GTGTCATCAG AGTCCAAACT TTCTGACTCC TGGCAGCCAC 
     4441 GCTCCCTGGA TATTGAAGTT GCAGCCTATG CACTGCTCTC ACACTTCTTA CAATTTCAGA 
     4501 CTTCTGAGGG AATCCCAATT ATGAGGTGGC TAAGCAGGCA AAGAAATAGC TTGGGTGGTT 
     4561 TTGCATCTAC TCAGGATACC ACTGTGGCTT TAAAGGCTCT GTCTGAATTT GCAGCCCTAA 
     4621 TGAATACAGA AAGGACAAAT ATCCAAGTGA CCGTGACGGG GCCTAGCTCA CCAAGTCCTC 
     4681 TTGCTGTGGT ACAGCCAACG GCAGTTAATA TTTCCGCAAA TGGTTTTGGA TTTGCTATTT 
     4741 GTCAGCTCAA TGTTGTATAT AATGTGAAGG CTTCTGGGTC TTCTAGAAGA CGAAGATCTA 
     4801 TCCAAAATCA AGAAGCCTTT GATTTAGATG TTGCTGTAAA AGAAAATAAA GATGATCTCA 
     4861 ATCATGTGGA TTTGAATGTG TGTACAAGCT TTTCGGGCCC GGGTAGGAGT GGCATGGCTC 
     4921 TTATGGAAGT TAACCTATTA AGTGGCTTTA TGGTGCCTTC AGAAGCAATT TCTCTGAGCG 
     4981 AGACAGTGAA GAAAGTGGAA TATGATCATG GAAAACTCAA CCTCTATTTA GATTCTGTAA 
     5041 ATGAAACCCA GTTTTGTGTT AATATTCCTG CTGTGAGAAA CTTTAAAGTT TCAAATACCC 
     5101 AAGATGCTTC AGTGTCCATA GTGGATTACT ATGAGCCAAG GAGACAGGCG GTGAGAAGTT 
     5161 ACAACTCTGA AGTGAAGCTG TCCTCCTGTG ACCTTTGCAG TGATGTCCAG GGCTGCCGTC 
     5221 CTTGTGAGGA TGGAGCTTCA GGCTCCCATC ATCACTCTTC AGTCATTTTT ATTTTCTGTT 
     5281 TCAAGCTTCT GTACTTTATG GAACTTTGGC TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5341 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5401 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5461 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5521 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5581 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5641 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5701 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5761 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5821 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5881 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5941 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     6001 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6061 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6121 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6181 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6241 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6301 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6361 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6421 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6481 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6541 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6601 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6661 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6721 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6781 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6841 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6901 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6961 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     7021 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7081 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7141 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7201 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7261 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7321 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7381 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7441 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7501 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7561 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7621 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7681 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7741 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7801 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7861 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7921 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7981 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     8041 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8101 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8161 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8221 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8281 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8341 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8401 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8461 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8521 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8581 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8641 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8701 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8761 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8821 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8881 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8941 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     9001 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9061 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9121 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//