LOCUS       RC228679                9018 bp    DNA     circular  SYN 
DEFINITION  KDM5C (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 5C (KDM5C), transcript variant 2
ACCESSION   NM_001146702
VERSION     NM_001146702.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5175..5266 
                     /label="Myc-DDK"  
     misc_feature    5296..5881 
                     /label="PolyA signal"  
     misc_feature    5353..5372 
                     /label="Restriction Enzyme"  
     misc_feature    6157..6829 
                     /label="ColE1"  
     misc_feature    7259..8053 
                     /label="Kan/Neo"  
     misc_feature    8088..8390 
                     /label="SV40 ori"  
     misc_feature    8476..8953 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5157..5163 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5157 
                     /label="KDM5C"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGGGG TCCGACGATT TCCTACCGCC ACCGGAGTGC CCGGTGTTCG 
     1081 AGCCTAGCTG GGCCGAGTTC CGAGACCCTC TTGGCTACAT CGCGAAAATC AGGCCCATCG 
     1141 CAGAGAAATC GGGCATTTGC AAGATCCGCC CACCCGCGAT TGTGGTGGAG GAAGGTGGTT 
     1201 ATGAAGCTAT CTGCAAGGAC CGTCGGTGGG CTCGGGTAGC CCAGCGCCTC AACTATCCAC 
     1261 CAGGCAAAAA TATTGGCTCC TTGCTACGCT CCCACTACGA ACGCATTGTT TATCCCTATG 
     1321 AAATGTACCA GTCTGGAGCC AACCTTGTGC AGTGTAACAC ACGTCCATTT GATAATGAGG 
     1381 AGAAGGACAA GGAATACAAA CCCCACAGCA TCCCCCTACG ACAGTCTGTG CAGCCTTCCA 
     1441 AGTTCAACAG CTATGGCCGG CGGGCCAAGA GACTGCAGCC TGATCCGGAA CCCACAGAGG 
     1501 AAGACATTGA GAAGAATCCA GAGCTGAAAA AGCTACAGAT CTATGGGGCA GGCCCCAAGA 
     1561 TGATGGGCCT GGGCCTCATG GCCAAAGACA AGACTCTGCG GAAGAAAGAT AAGGAGGGGC 
     1621 CTGAGTGTCC CCCCACAGTA GTGGTGAAGG AGGAGTTAGG TGGGGATGTG AAGGTGGAGT 
     1681 CAACATCGCC TAAGACCTTC CTGGAGAGCA AGGAGGAGCT GAGTCACAGC CCAGAACCCT 
     1741 GCACCAAGAT GACCATGAGG CTACGGAGGA ACCACAGCAA TGCCCAGTTT ATTGAGTCAT 
     1801 ATGTCTGCCG GATGTGTTCT CGAGGGGATG AGGATGACAA GCTCCTGCTG TGTGATGGCT 
     1861 GTGATGACAA CTACCACATC TTCTGCCTGC TGCCTCCTCT GCCTGAGATC CCCAAGGGTG 
     1921 TCTGGCGGTG CCCAAAGTGT GTCATGGCGG AGTGTAAGCG GCCCCCAGAA GCCTTTGGCT 
     1981 TTGAGCAGGC TACCCGGGAA TACACTCTGC AGAGCTTTGG CGAGATGGCC GACTCCTTTA 
     2041 AAGCTGACTA CTTCAACATG CCCGTGCATA TGGTGCCCAC AGAACTTGTG GAGAAGGAGT 
     2101 TCTGGAGGCT GGTAAATAGC ATTGAGGAAG ATGTGACTGT TGAGTATGGA GCTGACATCC 
     2161 ATTCCAAAGA ATTTGGCAGC GGTTTCCCTG TCAGTGACAG TAAACGGCAC CTAACCCCCG 
     2221 AAGAGGAGGA GTATGCTACC AGTGGTTGGA ACCTAAATGT GATGCCGGTG TTGGAACAGT 
     2281 CTGTACTGTG CCACATCAAT GCAGATATCT CTGGCATGAA GGTGCCCTGG CTCTACGTGG 
     2341 GCATGGTCTT CTCAGCCTTT TGCTGGCATA TTGAGGATCA CTGGAGTTAC TCCATTAACT 
     2401 ACCTCCACTG GGGTGAGCCG AAGACCTGGT ATGGGGTGCC CTCACTTGCA GCAGAACATT 
     2461 TGGAAGAAGT GATGAAGAAG CTGACACCTG AACTATTTGA TAGCCAGCCT GACCTCCTGC 
     2521 ACCAACTTGT CACCCTCATG AATCCCAACA CCCTCATGTC CCATGGTGTG CCAGTTGTCC 
     2581 GCACAAACCA GTGTGCAGGA GAGTTTGTCA TCACCTTCCC CCGTGCTTAC CACAGCGGCT 
     2641 TCAACCAAGG CTACAACTTT GCCGAGGCTG TCAACTTTTG CACTGCTGAC TGGTTGCCTG 
     2701 CTGGGCGCCA GTGCATTGAG CACTACCGCC GGCTCCGGAG ATACTGCGTC TTCTCCCATG 
     2761 AGGAGCTTAT CTGCAAGATG GCTGCCTGCC CAGAGAAGCT AGACCTGAAC CTGGCGGCAG 
     2821 CTGTGCATAA GGAGATGTTC ATCATGGTGC AAGAAGAGCG GCGTCTACGA AAGGCCCTGC 
     2881 TGGAGAAGGG TATCACAGAG GCTGAGCGAG AGGCTTTCGA GCTGCTCCCA GATGATGAGC 
     2941 GCCAGTGTAT CAAGTGCAAG ACTACGTGTT TCCTGTCAGC CCTGGCCTGC TACGACTGCC 
     3001 CAGACGGCCT TGTCTGCCTT TCCCACATCA ATGATCTCTG CAAGTGCTCC AGTAGCCGGC 
     3061 AGTACCTGCG GTATCGGTAT ACCTTGGATG AGCTTCCTGC CATGCTGCAT AAGCTGAAGG 
     3121 TTCGGGCTGA GTCCTTTGAC ACCTGGGCCA ACAAAGTGCG AGTGGCCCTG GAGGTGGAGG 
     3181 ATGGGCGGAA GCGCAGCCTT GAAGAACTGA GGGCACTAGA GTCTGAAGCC CGTGAGCGGA 
     3241 GGTTTCCTAA TAGTGAGCTG CTGCAGCAAC TAAAGAACTG CCTGAGTGAG GCAGAGGCTT 
     3301 GCGTGTCCCG AGCTCTGGGA CTGGTCAGCG GCCAGGAAGC TGGCCCCCAC AGGGTGGCTG 
     3361 GTCTACAGAT GACCCTGACT GAGCTCCGGG CCTTTCTGGA CCAGATGAAC AACCTGCCTT 
     3421 GCGCCATGCA CCAGATTGGG GATGTCAAGG GTGTTCTGGA ACAGGTGGAG GCCTACCAGG 
     3481 CTGAGGCTCG TGAGGCCCTG GCCTCACTGC CCTCCAGTCC AGGGCTACTG CAGTCCCTGT 
     3541 TGGAGAGGGG GCGGCAGCTG GGGGTGGAGG TGCCTGAGGC CCAGCAGCTC CAGCGGCAGG 
     3601 TGGAACAGGC GCGATGGCTG GATGAGGTGA AACGCACACT GGCCCCCTCA GCCCGAAGGG 
     3661 GCACCTTGGC TGTCATGCGA GGACTGTTGG TCGCGGGTGC CAGTGTAGCC CCTAGCCCTG 
     3721 CTGTGGATAA AGCCCAGGCC GAGCTGCAGG AACTGCTGAC CATTGCTGAA CGCTGGGAGG 
     3781 AGAAAGCCCA CCTCTGCCTG GAGGCCAGGC AGAAGCATCC ACCAGCCACA CTTGAGGCCA 
     3841 TAATCCGTGA AGCGGAAAAC ATCCCTGTTC ACCTGCCCAA CATCCAGGCT CTCAAGGAGG 
     3901 CTCTTGCTAA GGCCCGGGCC TGGATTGCTG ATGTTGATGA GATCCAAAAT GGTGACCACT 
     3961 ACCCCTGCCT GGATGACTTG GAGGGCCTAG TAGCTGTGGG CCGGGACCTA CCTGTGGGGC 
     4021 TGGAGGAGCT GAGACAGCTA GAGCTACAGG TACTGACAGC GCACTCCTGG AGGGAGAAGG 
     4081 CCTCCAAGAC CTTCCTCAAG AAAAATTCTT GCTACACGCT GCTGGAGGTT CTCTGCCCAT 
     4141 GTGCAGATGC CGGCTCAGAC AGCACCAAGC GCAGCCGGTG GATGGAGAAG GAGCTGGGGT 
     4201 TGTACAAATC TGACACAGAG CTGCTGGGGC TGTCTGCGCA GGACCTCAGG GACCCAGGCT 
     4261 CTGTGATCGT GGCCTTCAAG GAGGGGGAAC AGAAGGAGAA GGAGGGTATC CTGCAGCTGC 
     4321 GTCGCACCAA TTCGGCCAAG CCCAGTCCAC TGGCATCATC GAGCACGGCC TCCTCTACAA 
     4381 CCTCTATCTG TGTGTGTGGG CAGGTGCTGG CTGGGGCGGG AGCTCTGCAG TGTGACCTGT 
     4441 GTCAGGACTG GTTCCATGGG CGGTGTGTGT CAGTGCCTCG CCTCCTCAGC TCTCCGAGGC 
     4501 CCAATCCCAC CTCATCCCCA CTGCTGGCCT GGTGGGAATG GGACACCAAA TTCCTGTGTC 
     4561 CACTGTGTAT GCGCTCAAGG CGCCCGCGCC TGGAGACCAT CCTGGCACTG CTGGTAGCCC 
     4621 TGCAGAGACT GCCTGTGCGG CTGCCCGAGG GCGAGGCCCT GCAGTGCCTC ACAGAGAGGG 
     4681 CCATCAGCTG GCAAGGCCGC GCCAGGCAGG CTCTGGCCTC TGAAGATGTG ACTGCTCTTT 
     4741 TGGGACGGCT GGCTGAGCTC CGCCAACGGC TACAGGCTGA ACCTAGACCT GAGGAGCCTC 
     4801 CTAACTACCC TGCAGCCCCT GCTTCTGACC CCCTCAGAGA GGGCAGTGGC AAGGATATGC 
     4861 CTAAGGTCCA GGGCTTACTG GAGAATGGAG ACAGTGTGAC CAGTCCTGAG AAGGTAGCCC 
     4921 CGGAGGAGGG CTCAGATCTG GAGCTGCTGT CCTCGCTGTT GCCACAGTTG ACTGGCCCTG 
     4981 TGTTGGAACT GCCTGAGGCA ACCCGGGCCC CCTTGGAGGA GCTCATGATG GAGGGGGACC 
     5041 TGCTCGAGGT GACCCTGGAT GAGAACCACA GCATCCCTGA GTCCCTTGAC TTTTGTATTC 
     5101 TGACTCCAAG GTATTGTTCA GACCTCAGCT CCTGGGGGCC GGCCCCTGGA GTCTTCCCTC 
     5161 CCTGGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5221 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5281 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5341 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5401 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5461 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5521 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5581 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5641 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5701 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5761 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5821 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5881 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5941 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6001 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6061 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6121 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6181 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6241 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6301 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6361 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6421 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6481 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6541 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6601 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6661 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6721 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6781 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6841 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6901 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6961 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7021 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7081 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7141 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7201 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7261 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7321 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7381 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7441 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7501 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7561 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7621 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7681 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7741 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7801 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7861 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7921 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7981 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8041 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8101 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8161 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8221 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8281 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8341 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8401 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8461 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8521 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8581 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8641 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8701 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8761 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8821 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8881 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8941 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9001 AAATTTAACG CGAATTTT
//