LOCUS       RC228678                9006 bp    DNA     circular  SYN 
DEFINITION  PNPLA6 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 6 (PNPLA6), transcript variant 1
ACCESSION   NM_001166111
VERSION     NM_001166111.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5163..5254 
                     /label="Myc-DDK"  
     misc_feature    5284..5869 
                     /label="PolyA signal"  
     misc_feature    5341..5360 
                     /label="Restriction Enzyme"  
     misc_feature    6145..6817 
                     /label="ColE1"  
     misc_feature    7247..8041 
                     /label="Kan/Neo"  
     misc_feature    8076..8378 
                     /label="SV40 ori"  
     misc_feature    8464..8941 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5145..5151 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5145 
                     /label="PNPLA6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCTCCG CTGCAAACTG GAATGATGGG GACATCGAGT CACGGGCTGG 
     1081 CTACGAACTC CTCGGGGGCG AAGGTGGCGG AGAGGGATGG GTTCCAGGAC GTCCTGGCGC 
     1141 CCGGGGAAGG CTCGGCGGGA CGGATTTGCG GTGCGCAGCC AGTGCCGTTC GTCCCTCAGG 
     1201 TGCTTGGCGT GATGATCGGG GCCGGAGTGG CGGTGGTGGT CACGGCCGTG CTCATCCTCC 
     1261 TGGTGGTGCG GAGGCTGCGA GTGCCAAAAA CCCCAGCCCC GGATGGCCCC CGGTATCGGT 
     1321 TCCGGAAGAG GGACAAAGTG CTCTTCTATG GCCGGAAGAT TATGCGGAAG GTGTCACAAT 
     1381 CCACCTCCTC CCTCGTGGAT ACCTCTGTCT CCGCCACCTC CCGGCCACGC ATGAGGAAGA 
     1441 AACTGAAGAT GCTCAACATT GCCAAGAAGA TCCTGCGCAT CCAGAAAGAG ACGCCCACGC 
     1501 TGCAGCGGAA GGAGCCCCCG CCCGCAGTGC TAGAAGCTGA CCTGACCGAG GGCGACCTGG 
     1561 CTAACTCCCA TCTGCCCTCT GAAGTGCTTT ATATGCTCAA GAACGTCCGG GTGCTGGGCC 
     1621 ACTTCGAGAA GCCACTCTTC CTGGAGCTCT GCCGCCACAT GGTCTTCCAG CGGCTGGGCC 
     1681 AGGGTGACTA CGTCTTCCGG CCGGGCCAGC CAGATGCCAG CATCTACGTG GTGCAGGACG 
     1741 GGCTGCTGGA GCTCTGTCTG CCAGGGCCTG ACGGGAAGGA GTGTGTGGTG AAGGAAGTGG 
     1801 TTCCTGGGGA CAGCGTCAAC AGCCTTCTCA GCATCCTGGA TGTCATCACC GGTCACCAGC 
     1861 ATCCCCAGCG GACCGTGTCT GCCCGGGCGG CCCGGGACTC CACGGTGCTG CGCCTGCCGG 
     1921 TGGAAGCATT CTCCGCGGTC TTCACCAAGT ACCCGGAGAG CTTGGTGCGG GTCGTGCAGA 
     1981 TCATCATGGT GCGGCTGCAG CGAGTCACCT TCCTGGCACT GCACAACTAC CTGGGTCTGA 
     2041 CCAATGAGCT CTTCAGCCAC GAGATCCAGC CCCTGCGTCT GTTCCCCAGC CCCGGCCTCC 
     2101 CAACTCGCAC CAGCCCTGTG CGGGGCTCCA AGAGAATGGT CAGCACCTCA GCTACAGACG 
     2161 AGCCCAGGGA GACCCCAGGG CGGCCACCCG ATCCCACCGG GGCCCCGCTG CCTGGACCTA 
     2221 CAGGGGACCC TGTGAAGCCC ACATCCCTGG AAACCCCCTC GGCCCCTCTG CTGAGCCGCT 
     2281 GCGTCTCCAT GCCAGGGGAC ATCTCAGGCT TGCAGGGTGG CCCCCGCTCC GACTTCGACA 
     2341 TGGCCTATGA GCGTGGCCGG ATCTCCGTGT CCCTGCAGGA AGAGGCCTCC GGGGGGTCCC 
     2401 TGGCAGCCCC CGCTCGGACC CCCACTCAGG AGCCTCGTGA GCAGCCGGCA GGCGCCTGTG 
     2461 AATACAGCTA CTGTGAGGAT GAGTCGGCCA CTGGTGGCTG CCCTTTCGGG CCCTACCAGG 
     2521 GCCGCCAGAC CAGCAGCATC TTCGAGGCAG CAAAGCAGGA GCTGGCCAAG CTGATGCGGA 
     2581 TTGAGGACCC CTCCCTCCTG AACAGCAGAG TCTTGCTGCA CCACGCCAAA GCTGGCACCA 
     2641 TCATTGCCCG CCAGGGAGAC CAGGACGTGA GCCTGCACTT CGTGCTCTGG GGCTGCCTGC 
     2701 ACGTGTACCA GCGCATGATC GACAAGGCGG AGGACGTGTG CCTGTTCGTA GCGCAGCCCG 
     2761 GGGAACTGGT GGGGCAGCTG GCGGTGCTCA CTGGCGAACC TCTCATCTTC ACACTGCGAG 
     2821 CCCAACGCGA CTGCACCTTC CTGCGGATCT CCAAGTCCGA CTTCTATGAG ATCATGCGCG 
     2881 CACAGCCCAG TGTGGTGCTG AGTGCGGCGC ACACGGTGGC AGCCAGGATG TCGCCCTTCG 
     2941 TGCGCCAGAT GGACTTCGCC ATCGACTGGA CTGCAGTGGA GGCGGGACGC GCGCTGTACA 
     3001 GGCAGGGCGA CCGCTCCGAC TGCACTTACA TCGTGCTCAA TGGGCGGCTG CGTAGCGTGA 
     3061 TCCAGCGAGG CAGTGGCAAG AAGGAGCTGG TGGGCGAGTA CGGCCGCGGC GACCTCATCG 
     3121 GCGTGGTGGA GGCACTGACC CGGCAGCCGC GAGCCACGAC GGTGCACGCG GTGCGCGACA 
     3181 CGGAGCTGGC CAAGCTTCCC GAGGGCACCT TGGGTCACAT CAAACGCCGG TACCCGCAGG 
     3241 TCGTGACCCG CCTTATCCAC CTACTGAGCC AGAAAATTCT AGGGAATTTG CAGCAGCTGC 
     3301 AAGGACCCTT CCCAGCAGGC TCTGGGTTGG GTGTGCCCCC ACACTCGGAA CTCACCAACC 
     3361 CAGCCAGCAA CCTGGCAACT GTGGCAATCC TGCCTGTGTG TGCTGAGGTC CCCATGGTGG 
     3421 CCTTCACGCT GGAGCTGCAG CACGCCCTGC AGGCCATCGG TCCGACGCTA CTCCTTAACA 
     3481 GTGACATCAT CCGGGCACGC CTGGGGGCCT CCGCACTGGA TAGCATCCAA GAGTTCCGGC 
     3541 TGTCAGGGTG GCTGGCCCAG CAGGAGGATG CACACCGTAT CGTACTCTAC CAGACGGACG 
     3601 CCTCGCTGAC GCCCTGGACC GTGCGCTGCC TGCGACAGGC CGACTGCATC CTCATTGTGG 
     3661 GCCTGGGGGA CCAGGAGCCT ACCCTCGGCC AGCTGGAGCA GATGCTGGAG AACACGGCTG 
     3721 TGCGCGCCCT TAAGCAGCTA GTCCTGCTCC ACCGAGAGGA GGGCGCGGGC CCCACGCGCA 
     3781 CCGTGGAGTG GCTAAATATG CGCAGCTGGT GCTCGGGGCA CCTGCACCTG CGCTGTCCGC 
     3841 GCCGCCTCTT TTCGCGCCGC AGCCCTGCCA AGCTGCATGA GCTCTACGAG AAGGTTTTCT 
     3901 CCAGGCGCGC GGACCGGCAC AGCGACTTCT CCCGCTTGGC GAGGGTGCTC ACGGGGAACA 
     3961 CCATTGCCCT TGTGCTAGGC GGGGGCGGGG CCAGGGGCTG CTCGCACATC GGAGTACTAA 
     4021 AGGCATTAGA GGAGGCGGGG GTCCCCGTGG ACCTGGTGGG CGGCACGTCC ATTGGCTCTT 
     4081 TCATCGGAGC GTTGTACGCG GAGGAGCGCA GCGCCAGCCG CACGAAGCAG CGGGCCCGGG 
     4141 AGTGGGCCAA GAGCATGACT TCGGTGCTGG AACCTGTGTT GGACCTCACG TACCCAGTCA 
     4201 CCTCCATGTT CACTGGGTCT GCCTTTAACC GCAGCATCCA TCGGGTCTTC CAGGATAAGC 
     4261 AGATTGAGGA CCTGTGGCTG CCTTACTTCA ACGTGACCAC AGATATCACC GCCTCAGCCA 
     4321 TGCGAGTCCA CAAAGATGGC TCCCTGTGGC GGTACGTGCG CGCCAGCATG ACGCTGTCGG 
     4381 GCTACCTGCC CCCGCTGTGC GACCCCAAGG ACGGGCACCT ACTCATGGAT GGCGGCTACA 
     4441 TCAACAATCT GCCAGCGGAC ATCGCCCGCA GCATGGGTGC CAAAACGGTC ATCGCCATTG 
     4501 ACGTGGGGAG CCAGGATGAG ACGGACCTCA GCACCTACGG GGACAGCCTG TCCGGCTGGT 
     4561 GGCTGCTGTG GAAGCGGCTG AATCCCTGGG CTGACAAGGT AAAGGTTCCA GACATGGCTG 
     4621 AAATCCAGTC CCGCCTGGCC TACGTGTCCT GTGTGCGGCA GCTAGAGGTT GTCAAGTCCA 
     4681 GCTCCTACTG CGAGTACCTG CGCCCGCCCA TCGACTGCTT CAAGACCATG GACTTTGGGA 
     4741 AGTTCGACCA GATCTATGAT GTGGGCTACC AGTACGGGAA GGCGGTGTTT GGAGGCTGGA 
     4801 GCCGTGGCAA CGTCATTGAG AAAATGCTCA CAGACCGGCG GTCTACAGAC CTTAATGAGA 
     4861 GCCGCCGTGC AGACGTGCTT GCCTTCCCAA GCTCTGGCTT CACTGACTTG GCAGAGATTG 
     4921 TGTCCCGGAT TGAGCCCCCC ACGAGCTATG TCTCTGATGG CTGTGCTGAC GGAGAGGAGT 
     4981 CAGATTGTCT GACAGAGTAT GAGGAGGACG CCGGACCCGA CTGCTCGAGG GATGAAGGGG 
     5041 GGTCCCCCGA GGGCGCAAGC CCCAGCACTG CCTCCGAGAT GGAGGAGGAG AAGTCGATTC 
     5101 TCCGGCAACG ACGCTGTCTG CCCCAGGAGC CGCCCGGCTC AGCCACAGAT GCCACGCGTA 
     5161 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5221 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5281 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5341 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5401 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5461 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5521 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5581 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5641 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5701 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5761 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5821 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5881 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5941 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6001 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6061 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6121 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6181 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6241 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6301 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6361 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6421 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6481 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6541 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6601 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6661 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6721 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6781 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6841 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6901 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6961 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7021 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7081 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7141 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7201 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7261 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7321 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7381 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7441 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7501 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7561 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7621 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7681 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7741 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7801 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7861 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7921 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7981 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8041 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8101 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8161 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8221 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8281 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8341 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8401 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8461 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8521 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8581 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8641 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8701 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8761 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8821 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8881 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8941 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9001 AATTTT
//