LOCUS       RC228677                8985 bp    DNA     circular  SYN 
DEFINITION  CD109 (Myc-DDK-tagged)-Human CD109 molecule (CD109), transcript variant 3
ACCESSION   NM_001159588
VERSION     NM_001159588.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5142..5233 
                     /label="Myc-DDK"  
     misc_feature    5263..5848 
                     /label="PolyA signal"  
     misc_feature    5320..5339 
                     /label="Restriction Enzyme"  
     misc_feature    6124..6796 
                     /label="ColE1"  
     misc_feature    7226..8020 
                     /label="Kan/Neo"  
     misc_feature    8055..8357 
                     /label="SV40 ori"  
     misc_feature    8443..8920 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5124..5130 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5124 
                     /label="CD109"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGGCCCA CCGCTCCTGA CCGCCGCCCA CCTCCTCTGC GTGTGCACCG 
     1081 CCGCGCTGGC CGTGGCTCCC GGGCCTCGGT TTCTGGTGAC AGCCCCAGGG ATCATCAGGC 
     1141 CCGGAGGAAA TGTGACTATT GGGGTGGAGC TTCTGGAACA CTGCCCTTCA CAGGTGACTG 
     1201 TGAAGGCGGA GCTGCTCAAG ACAGCATCAA ACCTCACTGT CTCTGTCCTG GAAGCAGAAG 
     1261 GAGTCTTTGA AAAAGGCTCT TTTAAGACAC TTACTCTTCC ATCAGACCCC AAATCAAATT 
     1321 TGATCCAACA GTGGTTGTCA CAACAAAGTG ATCTTGGAGT CATTTCCAAA ACTTTTCAGC 
     1381 TATCTTCCCA TCCAATACTT GGTGACTGGT CTATTCAAGT TCAAGTGAAT GACCAGACAT 
     1441 ATTATCAATC ATTTCAGGTT TCAGAATATG TATTACCAAA ATTTGAAGTG ACTTTGCAGA 
     1501 CACCATTATA TTGTTCTATG AATTCTAAGC ATTTAAATGG TACCATCACG GCAAAGTATA 
     1561 CATATGGGAA GCCAGTGAAA GGAGACGTAA CGCTTACATT TTTACCTTTA TCCTTTTGGG 
     1621 GAAAGAAGAA AAATATTACA AAAACATTTA AGATAAATGG ATCTGCAAAC TTCTCTTTTA 
     1681 ATGATGAAGA GATGAAAAAT GTAATGGATT CTTCAAATGG ACTTTCTGAA TACCTGGATC 
     1741 TATCTTCCCC TGGACCAGTA GAAATTTTAA CCACAGTGAC AGAATCAGTT ACAGGTATTT 
     1801 CAAGAAATGT AAGCACTAAT GTGTTCTTCA AGCAACATGA TTACATCATT GAGTTTTTTG 
     1861 ATTATACTAC TGTCTTGAAG CCATCTCTCA ACTTCACAGC CACTGTGAAG GTAACTCGTG 
     1921 CTGATGGCAA CCAACTGACT CTTGAAGAAA GAAGAAATAA TGTAGTCATA ACAGTGACAC 
     1981 AGAGAAACTA TACTGAGTAC TGGAGCGGAT CTAACAGTGG AAATCAGAAA ATGGAAGCTG 
     2041 TTCAGAAAAT AAATTATACT GTCCCCCAAA GTGGAACTTT TAAGATTGAA TTCCCAATCC 
     2101 TGGAGGATTC CAGTGAGCTA CAGTTGAAGG CCTATTTCCT TGGTAGTAAA AGTAGCATGG 
     2161 CAGTTCATAG TCTGTTTAAG TCTCCTAGTA AGACATACAT CCAACTAAAA ACAAGAGATG 
     2221 AAAATATAAA GGTGGGATCG CCTTTTGAGT TGGTGGTTAG TGGCAACAAA CGATTGAAGG 
     2281 AGTTAAGCTA TATGGTAGTA TCCAGGGGAC AGTTGGTGGC TGTAGGAAAA CAAAATTCAA 
     2341 CAATGTTCTC TTTAACACCA GAAAATTCTT GGACTCCAAA AGCCTGTGTA ATTGTGTATT 
     2401 ATATTGAAGA TGATGGGGAA ATTATAAGTG ATGTTCTAAA AATTCCTGTT CAGCTTGTTT 
     2461 TTAAAAATAA GATAAAGCTA TATTGGAGTA AAGTGAAAGC TGAACCATCT GAGAAAGTCT 
     2521 CTCTTAGGAT CTCTGTGACA CAGCCTGACT CCATAGTTGG GATTGTAGCT GTTGACAAAA 
     2581 GTGTGAATCT GATGAATGCC TCTAATGATA TTACAATGGA AAATGTGGTC CATGAGTTGG 
     2641 AACTTTATAA CACAGGATAT TATTTAGGCA TGTTCATGAA TTCTTTTGCA GTCTTTCAGG 
     2701 AATGTGGACT CTGGGTATTG ACAGATGCAA ACCTCACGAA GGATTATATT GATGGTGTTT 
     2761 ATGACAATGC AGAATATGCT GAGAGGTTTA TGGAGGAAAA TGAAGGACAT ATTGTAGATA 
     2821 TTCATGACTT TTCTTTGGGT AGCAGTCCAC ATGTCCGAAA GCATTTTCCA GAGACTTGGA 
     2881 TTTGGCTAGA CACCAACATG GGTTACAGGA TTTACCAAGA ATTTGAAGTA ACTGTACCTG 
     2941 ATTCTATCAC TTCTTGGGTG GCTACTGGTT TTGTGATCTC TGAGGACCTG GGTCTTGGAC 
     3001 TAACAACTAC TCCAGTGGAG CTCCAAGCCT TCCAACCATT TTTCATTTTT TTGAATCTTC 
     3061 CCTACTCTGT TATCAGAGGT GAAGAATTTG CTTTGGAAAT AACTATATTC AATTATTTGA 
     3121 AAGATGCCAC TGAGGTTAAG GTAATCATTG AGAAAAGTGA CAAATTTGAT ATTCTAATGA 
     3181 CTTCAAATGA AATAAATGCC ACAGGCCACC AGCAGACCCT TCTGGTTCCC AGTGAGGATG 
     3241 GGGCAACTGT TCTTTTTCCC ATCAGGCCAA CACATCTGGG AGAAATTCCT ATCACAGTCA 
     3301 CAGCTCTTTC ACCCACTGCT TCTGATGCTG TCACCCAGAT GATTTTAGTA AAGGCTGAAG 
     3361 GAATAGAAAA ATCATATTCA CAATCCATCT TATTAGACTT GACTGACAAT AGGCTACAGA 
     3421 GTACCCTGAA AACTTTGAGT TTCTCATTTC CTCCTAATAC AGTGACTGGC AGTGAAAGAG 
     3481 TTCAGATCAC TGCAATTGGA GATGTTCTTG GTCCTTCCAT CAATGGCTTA GCCTCATTGA 
     3541 TTCGGATGCC TTATGGCTGT GGTGAACAGA ACATGATAAA TTTTGCTCCA AATATTTACA 
     3601 TTTTGGATTA TCTGACTAAA AAGAAACAAC TGACAGATAA TTTGAAAGAA AAAGCTCTTT 
     3661 CATTTATGAG GCAAGGTTAC CAGAGAGAAC TTCTCTATCA GAGGGAAGAT GGCTCTTTCA 
     3721 GTGCTTTTGG GAATTATGAC CCTTCTGGGA GCACTTGGTT GTCAGCTTTT GTTTTAAGAT 
     3781 GTTTCCTTGA AGCCGATCCT TACATAGATA TTGATCAGAA TGTGTTACAC AGAACATACA 
     3841 CTTGGCTTAA AGGACATCAG AAATCCAACG GTGAATTTTG GGATCCAGGA AGAGTGATTC 
     3901 ATAGTGAGCT TCAAGGTGGC AATAAAAGTC CAGTAACACT TACAGCCTAT ATTGTAACTT 
     3961 CTCTCCTGGG ATATAGAAAG TATCAGCCTA ACATTGATGT GCAAGAGTCT ATCCATTTTT 
     4021 TGGAGTCTGA ATTCAGTAGA GGAATTTCAG ACAATTATAC TCTAGCCCTT ATAACTTATG 
     4081 CATTGTCATC AGTGGGGAGT CCTAAAGCGA AGGAAGCTTT GAATATGCTG ACTTGGAGAG 
     4141 CAGAACAAGA AGGTGGCATG CAATTCTGGG TGTCATCAGA GTCCAAACTT TCTGACTCCT 
     4201 GGCAGCCACG CTCCCTGGAT ATTGAAGTTG CAGCCTATGC ACTGCTCTCA CACTTCTTAC 
     4261 AATTTCAGAC TTCTGAGGGA ATCCCAATTA TGAGGTGGCT AAGCAGGCAA AGAAATAGCT 
     4321 TGGGTGGTTT TGCATCTACT CAGGATACCA CTGTGGCTTT AAAGGCTCTG TCTGAATTTG 
     4381 CAGCCCTAAT GAATACAGAA AGGACAAATA TCCAAGTGAC CGTGACGGGG CCTAGCTCAC 
     4441 CAAGTCCTGT AAAGTTTCTG ATTGACACAC ACAACCGCTT ACTCCTTCAG ACAGCAGAGC 
     4501 TTGCTGTGGT ACAGCCAATG GCAGTTAATA TTTCCGCAAA TGGTTTTGGA TTTGCTATTT 
     4561 GTCAGCTCAA TGTTGTATAT AATGTGAAGG CTTCTGGGTC TTCTAGAAGA CGAAGATCTA 
     4621 TCCAAAATCA AGAAGCCTTT GATTTAGATG TTGCTGTAAA AGAAAATAAA GATGATCTCA 
     4681 ATCATGTGGA TTTGAATGTG TGTACAAGCT TTTCGGGCCC GGGTAGGAGT GGCATGGCTC 
     4741 TTATGGAAGT TAACCTATTA AGTGGCTTTA TGGTGCCTTC AGAAGCAATT TCTCTGAGCG 
     4801 AGACAGTGAA GAAAGTGGAA TATGATCATG GAAAACTCAA CCTCTATTTA GATTCTGTAA 
     4861 ATGAAACCCA GTTTTGTGTT AATATTCCTG CTGTGAGAAA CTTTAAAGTT TCAAATACCC 
     4921 AAGATGCTTC AGTGTCCATA GTGGATTACT ATGAGCCAAG GAGACAGGCG GTGAGAAGTT 
     4981 ACAACTCTGA AGTGAAGCTG TCCTCCTGTG ACCTTTGCAG TGATGTCCAG GGCTGCCGTC 
     5041 CTTGTGAGGA TGGAGCTTCA GGCTCCCATC ATCACTCTTC AGTCATTTTT ATTTTCTGTT 
     5101 TCAAGCTTCT GTACTTTATG GAACTTTGGC TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5161 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5221 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5281 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5341 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5401 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5461 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5521 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5581 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5641 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5701 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5761 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5821 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5881 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5941 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6001 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6061 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6121 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6181 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6241 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6301 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6361 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6421 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6481 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6541 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6601 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6661 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6721 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6781 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6841 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6901 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6961 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7021 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7081 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7141 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7201 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7261 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7321 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7381 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7441 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7501 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7561 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7621 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7681 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7741 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7801 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7861 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7921 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7981 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8041 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8101 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8161 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8221 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8281 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8341 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8401 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8461 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8521 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8581 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8641 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8701 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8761 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8821 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8881 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8941 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//