LOCUS       RC228676                8976 bp    DNA     circular  SYN 
DEFINITION  PNPLA6 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 6 (PNPLA6), transcript variant 5
ACCESSION   NM_001166114
VERSION     NM_001166114.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5133..5224 
                     /label="Myc-DDK"  
     misc_feature    5254..5839 
                     /label="PolyA signal"  
     misc_feature    5311..5330 
                     /label="Restriction Enzyme"  
     misc_feature    6115..6787 
                     /label="ColE1"  
     misc_feature    7217..8011 
                     /label="Kan/Neo"  
     misc_feature    8046..8348 
                     /label="SV40 ori"  
     misc_feature    8434..8911 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5115..5121 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5115 
                     /label="PNPLA6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGACATCG AGTCACGGGC TGGCTACGAA CTCCTCGGGG GCGAAGGTGG 
     1081 CGGAGAGGGA TGGGTTCCAG GACGTCCTGG CGCCCGGGGA AGGCTCGGCG GGACGGATTT 
     1141 GCGGTGCGCA GCCAGTGCCG TTCGTCCCTC AGGTGCTTGG CGTGATGATC GGGGCCGGAG 
     1201 TGGCGGTGGT GGTCACGGCC GTGCTCATCC TCCTGGTGGT GCGGAGGCTG CGAGTGCCAA 
     1261 AAACCCCAGC CCCGGATGGC CCCCGGTATC GGTTCCGGAA GAGGGACAAA GTGCTCTTCT 
     1321 ATGGCCGGAA GATTATGCGG AAGGTGTCAC AATCCACCTC CTCCCTCGTG GATACCTCTG 
     1381 TCTCCGCCAC CTCCCGGCCA CGCATGAGGA AGAAACTGAA GATGCTCAAC ATTGCCAAGA 
     1441 AGATCCTGCG CATCCAGAAA GAGACGCCCA CGCTGCAGCG GAAGGAGCCC CCGCCCGCAG 
     1501 TGCTAGAAGC TGACCTGACC GAGGGCGACC TGGCTAACTC CCATCTGCCC TCTGAAGTGC 
     1561 TTTATATGCT CAAGAACGTC CGGGTGCTGG GCCACTTCGA GAAGCCACTC TTCCTGGAGC 
     1621 TCTGCCGCCA CATGGTCTTC CAGCGGCTGG GCCAGGGTGA CTACGTCTTC CGGCCGGGCC 
     1681 AGCCAGATGC CAGCATCTAC GTGGTGCAGG ACGGGCTGCT GGAGCTCTGT CTGCCAGGGC 
     1741 CTGACGGGAA GGAGTGTGTG GTGAAGGAAG TGGTTCCTGG GGACAGCGTC AACAGCCTTC 
     1801 TCAGCATCCT GGATGTCATC ACCGGTCACC AGCATCCCCA GCGGACCGTG TCTGCCCGGG 
     1861 CGGCCCGGGA CTCCACGGTG CTGCGCCTGC CGGTGGAAGC ATTCTCCGCG GTCTTCACCA 
     1921 AGTACCCGGA GAGCTTGGTG CGGGTCGTGC AGATCATCAT GGTGCGGCTG CAGCGAGTCA 
     1981 CCTTCCTGGC ACTGCACAAC TACCTGGGTC TGACCAATGA GCTCTTCAGC CACGAGATCC 
     2041 AGCCCCTGCG TCTGTTCCCC AGCCCCGGCC TCCCAACTCG CACCAGCCCT GTGCGGGGCT 
     2101 CCAAGAGAAT GGTCAGCACC TCAGCTACAG ACGAGCCCAG GGAGACCCCA GGGCGGCCAC 
     2161 CCGATCCCAC CGGGGCCCCG CTGCCTGGAC CTACAGGGGA CCCTGTGAAG CCCACATCCC 
     2221 TGGAAACCCC CTCGGCCCCT CTGCTGAGCC GCTGCGTCTC CATGCCAGGG GACATCTCAG 
     2281 GCTTGCAGGG TGGCCCCCGC TCCGACTTCG ACATGGCCTA TGAGCGTGGC CGGATCTCCG 
     2341 TGTCCCTGCA GGAAGAGGCC TCCGGGGGGT CCCTGGCAGC CCCCGCTCGG ACCCCCACTC 
     2401 AGGAGCCTCG TGAGCAGCCG GCAGGCGCCT GTGAATACAG CTACTGTGAG GATGAGTCGG 
     2461 CCACTGGTGG CTGCCCTTTC GGGCCCTACC AGGGCCGCCA GACCAGCAGC ATCTTCGAGG 
     2521 CAGCAAAGCA GGAGCTGGCC AAGCTGATGC GGATTGAGGA CCCCTCCCTC CTGAACAGCA 
     2581 GAGTCTTGCT GCACCACGCC AAAGCTGGCA CCATCATTGC CCGCCAGGGA GACCAGGACG 
     2641 TGAGCCTGCA CTTCGTGCTC TGGGGCTGCC TGCACGTGTA CCAGCGCATG ATCGACAAGG 
     2701 CGGAGGACGT GTGCCTGTTC GTAGCGCAGC CCGGGGAACT GGTGGGGCAG CTGGCGGTGC 
     2761 TCACTGGCGA ACCTCTCATC TTCACACTGC GAGCCCAACG CGACTGCACC TTCCTGCGGA 
     2821 TCTCCAAGTC CGACTTCTAT GAGATCATGC GCGCACAGCC CAGTGTGGTG CTGAGTGCGG 
     2881 CGCACACGGT GGCAGCCAGG ATGTCGCCCT TCGTGCGCCA GATGGACTTC GCCATCGACT 
     2941 GGACTGCAGT GGAGGCGGGA CGCGCGCTGT ACAGGCAGGG CGACCGCTCC GACTGCACTT 
     3001 ACATCGTGCT CAATGGGCGG CTGCGTAGCG TGATCCAGCG AGGCAGTGGC AAGAAGGAGC 
     3061 TGGTGGGCGA GTACGGCCGC GGCGACCTCA TCGGCGTGGT GGAGGCACTG ACCCGGCAGC 
     3121 CGCGAGCCAC GACGGTGCAC GCGGTGCGCG ACACGGAGCT GGCCAAGCTT CCCGAGGGCA 
     3181 CCTTGGGTCA CATCAAACGC CGGTACCCGC AGGTCGTGAC CCGCCTTATC CACCTACTGA 
     3241 GCCAGAAAAT TCTAGGGAAT TTGCAGCAGC TGCAAGGACC CTTCCCAGGC TCTGGGTTGG 
     3301 GTGTGCCCCC ACACTCGGAA CTCACCAACC CAGCCAGCAA CCTGGCAACT GTGGCAATCC 
     3361 TGCCTGTGTG TGCTGAGGTC CCCATGGTGG CCTTCACGCT GGAGCTGCAG CACGCCCTGC 
     3421 AGGCCATCGG TCCGACGCTA CTCCTTAACA GTGACATCAT CCGGGCACGC CTGGGGGCCT 
     3481 CCGCACTGGA TAGCATCCAA GAGTTCCGGC TGTCAGGGTG GCTGGCCCAG CAGGAGGATG 
     3541 CACACCGTAT CGTACTCTAC CAGACGGACG CCTCGCTGAC GCCCTGGACC GTGCGCTGCC 
     3601 TGCGACAGGC CGACTGCATC CTCATTGTGG GCCTGGGGGA CCAGGAGCCT ACCCTCGGCC 
     3661 AGCTGGAGCA GATGCTGGAG AACACGGCTG TGCGCGCCCT TAAGCAGCTA GTCCTGCTCC 
     3721 ACCGAGAGGA GGGCGCGGGC CCCACGCGCA CCGTGGAGTG GCTAAATATG CGCAGCTGGT 
     3781 GCTCGGGGCA CCTGCACCTG CGCTGTCCGC GCCGCCTCTT TTCGCGCCGC AGCCCTGCCA 
     3841 AGCTGCATGA GCTCTACGAG AAGGTTTTCT CCAGGCGCGC GGACCGGCAC AGCGACTTCT 
     3901 CCCGCTTGGC GAGGGTGCTC ACGGGGAACA CCATTGCCCT TGTGCTAGGC GGGGGCGGGG 
     3961 CCAGGGGCTG CTCGCACATC GGAGTACTAA AGGCATTAGA GGAGGCGGGG GTCCCCGTGG 
     4021 ACCTGGTGGG CGGCACGTCC ATTGGCTCTT TCATCGGAGC GTTGTACGCG GAGGAGCGCA 
     4081 GCGCCAGCCG CACGAAGCAG CGGGCCCGGG AGTGGGCCAA GAGCATGACT TCGGTGCTGG 
     4141 AACCTGTGTT GGACCTCACG TACCCAGTCA CCTCCATGTT CACTGGGTCT GCCTTTAACC 
     4201 GCAGCATCCA TCGGGTCTTC CAGGATAAGC AGATTGAGGA CCTGTGGCTG CCTTACTTCA 
     4261 ACGTGACCAC AGATATCACC GCCTCAGCCA TGCGAGTCCA CAAAGATGGC TCCCTGTGGC 
     4321 GGTACGTGCG CGCCAGCATG ACGCTGTCGG GCTACCTGCC CCCGCTGTGC GACCCCAAGG 
     4381 ACGGGCACCT ACTCATGGAT GGCGGCTACA TCAACAATCT GCCAGCGGAC ATCGCCCGCA 
     4441 GCATGGGTGC CAAAACGGTC ATCGCCATTG ACGTGGGGAG CCAGGATGAG ACGGACCTCA 
     4501 GCACCTACGG GGACAGCCTG TCCGGCTGGT GGCTGCTGTG GAAGCGGCTG AATCCCTGGG 
     4561 CTGACAAGGT AAAGGTTCCA GACATGGCTG AAATCCAGTC CCGCCTGGCC TACGTGTCCT 
     4621 GTGTGCGGCA GCTAGAGGTT GTCAAGTCCA GCTCCTACTG CGAGTACCTG CGCCCGCCCA 
     4681 TCGACTGCTT CAAGACCATG GACTTTGGGA AGTTCGACCA GATCTATGAT GTGGGCTACC 
     4741 AGTACGGGAA GGCGGTGTTT GGAGGCTGGA GCCGTGGCAA CGTCATTGAG AAAATGCTCA 
     4801 CAGACCGGCG GTCTACAGAC CTTAATGAGA GCCGCCGTGC AGACGTGCTT GCCTTCCCAA 
     4861 GCTCTGGCTT CACTGACTTG GCAGAGATTG TGTCCCGGAT TGAGCCCCCC ACGAGCTATG 
     4921 TCTCTGATGG CTGTGCTGAC GGAGAGGAGT CAGATTGTCT GACAGAGTAT GAGGAGGACG 
     4981 CCGGACCCGA CTGCTCGAGG GATGAAGGGG GGTCCCCCGA GGGCGCAAGC CCCAGCACTG 
     5041 CCTCCGAGAT GGAGGAGGAG AAGTCGATTC TCCGGCAACG ACGCTGTCTG CCCCAGGAGC 
     5101 CGCCCGGCTC AGCCACAGAT GCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5161 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5221 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5281 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5341 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5401 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5461 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5521 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5581 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5641 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5701 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5761 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5821 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5881 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5941 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6001 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6061 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6121 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6181 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6241 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6301 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6361 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6421 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6481 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6541 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6601 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6661 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6721 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6781 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6841 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6901 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6961 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7021 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7081 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7141 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7201 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7261 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7321 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7381 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7441 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7501 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7561 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7621 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7681 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7741 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7801 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7861 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7921 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7981 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8041 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8101 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8161 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8221 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8281 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8341 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8401 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8461 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8521 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8581 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8641 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8701 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8761 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8821 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8881 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8941 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//