LOCUS       RC228675                8937 bp    DNA     circular  SYN 
DEFINITION  TNIK (Myc-DDK-tagged)-Human TRAF2 and NCK interacting kinase (TNIK), transcript variant 2
ACCESSION   NM_001161560
VERSION     NM_001161560.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5094..5185 
                     /label="Myc-DDK"  
     misc_feature    5215..5800 
                     /label="PolyA signal"  
     misc_feature    5272..5291 
                     /label="Restriction Enzyme"  
     misc_feature    6076..6748 
                     /label="ColE1"  
     misc_feature    7178..7972 
                     /label="Kan/Neo"  
     misc_feature    8007..8309 
                     /label="SV40 ori"  
     misc_feature    8395..8872 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5076..5082 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5076 
                     /label="TNIK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGAC TCCCCGGCTC GAAGCCTGGA TGAAATAGAT CTCTCGGCTC 
     1081 TGAGGGACCC CGCAGGGATC TTTGAATTGG TGGAACTTGT TGGAAATGGA ACATACGGGC 
     1141 AAGTTTATAA GGGTCGTCAT GTCAAAACGG GCCAGCTTGC AGCCATCAAG GTTATGGATG 
     1201 TCACAGGGGA TGAAGAGGAA GAAATCAAAC AAGAAATTAA CATGTTGAAG AAATATTCTC 
     1261 ATCACCGGAA TATTGCTACA TACTATGGTG CTTTTATCAA AAAGAACCCA CCAGGCATGG 
     1321 ATGACCAACT TTGGTTGGTG ATGGAGTTTT GTGGTGCTGG CTCTGTCACC GACCTGATCA 
     1381 AGAACACAAA AGGTAACACG TTGAAAGAGG AGTGGATTGC ATACATCTGC AGGGAAATCT 
     1441 TACGGGGGCT GAGTCACCTG CACCAGCATA AAGTGATTCA TCGAGATATT AAAGGGCAAA 
     1501 ATGTCTTGCT GACTGAAAAT GCAGAAGTTA AACTAGTGGA CTTTGGAGTC AGTGCTCAGC 
     1561 TTGATCGAAC AGTGGGCAGG AGGAATACTT TCATTGGAAC TCCCTACTGG ATGGCACCAG 
     1621 AAGTTATTGC CTGTGATGAA AACCCAGATG CCACATATGA TTTCAAGAGT GACTTGTGGT 
     1681 CTTTGGGTAT CACCGCCATT GAAATGGCAG AAGGTGCTCC CCCTCTCTGT GACATGCACC 
     1741 CCATGAGAGC TCTCTTCCTC ATCCCCCGGA ACCCAGCGCC TCGGCTGAAG TCTAAGAAGT 
     1801 GGTCAAAAAA ATTCCAGTCA TTTATTGAGA GCTGCTTGGT AAAGAATCAC AGCCAGCGAC 
     1861 CAGCAACAGA ACAATTGATG AAGCATCCAT TTATACGAGA CCAACCTAAT GAGCGACAGG 
     1921 TCCGCATTCA ACTCAAGGAC CATATTGATA GAACAAAGAA GAAGCGAGGA GAAAAAGATG 
     1981 AGACAGAGTA TGAGTACAGT GGAAGTGAGG AAGAAGAGGA GGAGAATGAC TCAGGAGAGC 
     2041 CCAGCTCCAT CCTGAATCTG CCAGGGGAGT CGACGCTGCG GAGGGACTTT CTGAGGCTGC 
     2101 AGCTGGCCAA CAAGGAGCGT TCTGAGGCCC TACGGAGGCA GCAGCTGGAG CAGCAGCAGC 
     2161 GGGAGAATGA GGAGCACAAG CGGCAGCTGC TGGCCGAGCG TCAGAAGCGC ATCGAGGAGC 
     2221 AGAAAGAGCA GAGGCGGCGG CTGGAGGAGC AACAAAGGCG AGAGAAGGAG CTGCGGAAGC 
     2281 AGCAGGAGAG GGAGCAGCGC CGGCACTATG AGGAGCAGAT GCGCCGGGAG GAGGAGAGGA 
     2341 GGCGTGCGGA GCATGAACAG GAATACATCA GGCGACAGTT AGAGGAGGAG CAGAGACAGT 
     2401 TAGAGATCTT GCAGCAGCAG CTACTGCATG AACAAGCTCT ACTTCTGGAA TATAAGCGCA 
     2461 AACAATTGGA AGAACAGAGA CAAGCAGAAA GACTGCAGAG GCAGCTAAAG CAAGAAAGAG 
     2521 ACTACTTAGT TTCCCTTCAG CATCAGCGGC AGGAGCAGAG GCCTGTGGAG AAGAAGCCAC 
     2581 TGTACCATTA CAAAGAAGGA ATGAGTCCTA GTGAGAAGCC AGCATGGGCC AAGGAGGTAG 
     2641 AAGAACGGTC AAGGCTCAAC CGGCAAAGTT CCCCTGCCAT GCCTCACAAG GTTGCCAACA 
     2701 GGATATCTGA CCCCAACCTG CCCCCAAGGT CGGAGTCCTT CAGCATTAGT GGAGTTCAGC 
     2761 CTGCTCGAAC ACCCCCCATG CTCAGACCAG TCGATCCCCA GATCCCACAT CTGGTAGCTG 
     2821 TAAAATCCCA GGGACCTGCC TTGACCGCCT CCCAGTCAGT GCACGAGCAG CCCACAAAGG 
     2881 GCCTCTCTGG GTTTCAGGAG GCTCTGAACG TGACCTCCCA CCGCGTGGAG ATGCCACGCC 
     2941 AGAACTCAGA TCCCACCTCG GAAAATCCTC CTCTCCCCAC TCGCATTGAA AAGTTTGACC 
     3001 GAAGCTCTTG GTTACGACAG GAAGAAGACA TTCCACCAAA GGTGCCTCAA AGAACAACTT 
     3061 CTATATCCCC AGCATTAGCC AGAAAGAATT CTCCTGGGAA TGGTAGTGCT CTGGGACCCA 
     3121 GACTAGGATC TCAACCCATC AGAGCAAGCA ACCCTGATCT CCGGAGAACT GAGCCCATCT 
     3181 TGGAGAGCCC CTTGCAGAGG ACCAGCAGTG GCAGTTCCTC CAGCTCCAGC ACCCCTAGCT 
     3241 CCCAGCCCAG CTCCCAAGGA GGCTCCCAGC CTGGATCACA AGCAGGATCC AGTGAACGCA 
     3301 CCAGAGTTCG AGCCAACAGT AAGTCAGAAG GATCACCTGT GCTCCCCCAT GAGCCTGCCA 
     3361 AGGTGAAACC AGAAGAATCC AGGGACATTA CCCGGCCCAG TCGACCAGCT GATCTGACGG 
     3421 CATTAGCCAA AGAACTAAGA GAACTCCGGA TTGAAGAAAC AAACCGCCCA ATGAAGAAGG 
     3481 TGACTGATTA CTCCTCCTCC AGTGAGGAGT CAGAAAGTAG CGAGGAAGAG GAGGAAGATG 
     3541 GAGAGAGCGA GACCCATGAT GGGACAGTGG CTGTCAGCGA CATACCCAGA CTGATACCAA 
     3601 CAGGAGCTCC AGGCAGCAAC GAGCAGTACA ATGTGGGAAT GGTGGGGACG CATGGGCTGG 
     3661 AGACCTCTCA TGCGGACAGT TTCAGCGGCA GTATTTCAAG AGAAGGAACC TTGATGATTA 
     3721 GAGAGACGTC TGGAGAGAAG AAGCGATCTG GCCACAGTGA CAGCAATGGC TTTGCTGGCC 
     3781 ACATCAACCT CCCTGACCTG GTGCAGCAGA GCCATTCTCC AGCTGGAACC CCGACTGAGG 
     3841 GACTGGGGCG CGTCTCAACC CATTCCCAGG AGATGGACTC TGGGACTGAA TATGGCATGG 
     3901 GGAGCAGCAC CAAAGCCTCC TTCACCCCCT TTGTGGACCC CAGAGTATAC CAGACGTCTC 
     3961 CCACTGATGA AGATGAAGAG GATGAGGAAT CATCAGCCGC AGCTCTGTTT ACTAGCGAAC 
     4021 TTCTTAGGCA AGAACAGGCC AAACTCAATG AAGCAAGAAA GATTTCGGTG GTAAATGTAA 
     4081 ACCCAACCAA CATTCGGCCT CATAGCGACA CACCAGAAAT CAGAAAATAC AAGAAACGAT 
     4141 TCAACTCAGA AATACTTTGT GCAGCTCTGT GGGGTGTAAA CCTTCTGGTG GGGACTGAAA 
     4201 ATGGCCTGAT GCTTTTGGAC CGAAGTGGGC AAGGCAAAGT CTATAATCTG ATCAACCGGA 
     4261 GGCGATTTCA GCAGATGGAT GTGCTAGAGG GACTGAATGT CCTTGTGACA ATTTCAGGAA 
     4321 AGAAGAATAA GCTACGAGTT TACTATCTTT CATGGTTAAG AAACAGAATA CTACATAATG 
     4381 ACCCAGAAGT AGAAAAGAAA CAAGGCTGGA TCACTGTTGG GGACTTGGAA GGCTGTATAC 
     4441 ATTATAAAGT TGTTAAATAT GAAAGGATCA AATTTTTGGT GATTGCCTTA AAGAATGCTG 
     4501 TGGAAATATA TGCTTGGGCT CCTAAACCGT ATCATAAATT CATGGCATTT AAGTCTTTTG 
     4561 CAGATCTCCA GCACAAGCCT CTGCTAGTTG ATCTCACGGT AGAAGAAGGT CAAAGATTAA 
     4621 AGGTTATTTT TGGTTCACAC ACTGGTTTCC ATGTAATTGA TGTTGATTCA GGAAACTCTT 
     4681 ATGATATCTA CATACCATCT CATATTCAGG GCAATATCAC TCCTCATGCT ATTGTCATCT 
     4741 TGCCTAAAAC AGATGGAATG GAAATGCTTG TTTGCTATGA GGATGAGGGG GTGTATGTAA 
     4801 ACACCTATGG CCGGATAACT AAGGATGTGG TGCTCCAATG GGGAGAAATG CCCACGTCTG 
     4861 TGGCCTACAT TCATTCCAAT CAGATAATGG GCTGGGGCGA GAAAGCTATT GAGATCCGGT 
     4921 CAGTGGAAAC AGGACATTTG GATGGAGTAT TTATGCATAA GCGAGCTCAA AGGTTAAAGT 
     4981 TTCTATGTGA AAGAAATGAT AAGGTATTTT TTGCATCCGT GCGATCTGGA GGAAGTAGCC 
     5041 AAGTGTTTTT CATGACCCTC AACAGAAATT CCATGATGAA CTGGACGCGT ACGCGGCCGC 
     5101 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5161 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5221 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5281 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5341 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5401 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5461 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5521 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5581 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5641 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5701 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5761 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5821 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5881 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5941 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6001 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6061 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6121 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6181 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6241 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6301 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6361 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6421 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6481 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6541 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6601 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6661 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6721 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6781 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6841 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6901 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6961 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7021 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7081 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7141 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7201 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7261 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7321 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7381 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7441 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7501 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7561 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7621 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7681 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7741 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7801 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7861 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7921 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7981 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8041 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8101 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8161 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8221 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8281 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8341 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8401 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8461 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8521 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8581 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8641 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8701 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8761 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8821 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8881 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//