LOCUS       RC228674                8907 bp    DNA     circular  SYN 
DEFINITION  LTBP1 (Myc-DDK-tagged)-Human latent transforming growth factor beta binding protein 1 (LTBP1), transcript variant 3
ACCESSION   NM_001166265
VERSION     NM_001166265.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5064..5155 
                     /label="Myc-DDK"  
     misc_feature    5185..5770 
                     /label="PolyA signal"  
     misc_feature    5242..5261 
                     /label="Restriction Enzyme"  
     misc_feature    6046..6718 
                     /label="ColE1"  
     misc_feature    7148..7942 
                     /label="Kan/Neo"  
     misc_feature    7977..8279 
                     /label="SV40 ori"  
     misc_feature    8365..8842 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5046..5052 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5046 
                     /label="LTBP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATACTAAG CTGATGTGTT TGTTGTTCTT TTTCTCCCTG CCTCCGCTCC 
     1081 TAGTGAGTAA CCACACTGGC CGCATCAAGG TGGTCTTTAC TCCGAGCATC TGTAAAGTGA 
     1141 CCTGCACCAA GGGCAGCTGT CAGAACAGCT GTGAGAAGGG GAACACCACC ACTCTCATTA 
     1201 GTGAGAATGG TCATGCTGCC GACACCCTGA CGGCCACGAA CTTCCGAGTG GTAATTTGCC 
     1261 ATCTTCCATG TATGAATGGT GGCCAGTGCA GTTCAAGGGA CAAATGTCAG TGCCCTCCAA 
     1321 ATTTCACAGG AAAACTTTGT CAGATCCCAG TCCATGGTGC CAGCGTGCCT AAACTTTATC 
     1381 AGCATTCCCA GCAGCCAGGC AAGGCGTTGG GGACGCATGT CATCCATTCA ACACATACCT 
     1441 TGCCTCTGAC CGTGACTAGC CAGCAAGGAG TCAAAGTGAA ATTTCCTCCT AACATAGTCA 
     1501 ATATCCATGT GAAACATCCT CCTGAAGCTT CCGTCCAGAT ACATCAGGTT TCAAGAATTG 
     1561 ATGGCCCAAC AGGCCAGAAG ACAAAAGAAG CTCAACCAGG CCAATCCCAA GTCTCGTACC 
     1621 AAGGGCTTCC TGTCCAGAAG ACCCAGACCA TACATTCCAC ATACTCCCAC CAGCAGGTCA 
     1681 TTCCTCACGT CTACCCCGTG GCTGCTAAGA CACAGCTTGG CCGGTGCTTC CAGGAAACCA 
     1741 TTGGGTCACA GTGTGGCAAA GCGCTCCCTG GCCTTTCAAA GCAAGAGGAC TGCTGTGGAA 
     1801 CTGTGGGTAC CTCCTGGGGC TTTAACAAAT GCCAGAAATG CCCCAAGAAA CCATCTTATC 
     1861 ATGGATACAA CCAAATGATG GAATGCCTAC CGGGTTATAA GCGGGTTAAC AACACCTTTT 
     1921 GCCAAGATAT TAATGAATGT CAGCTACAAG GTGTATGCCC TAATGGTGAG TGTTTGAATA 
     1981 CCATGGGCAG CTATCGATGT ACCTGCAAAA TAGGATTTGG GCCGGATCCT ACCTTTTCAA 
     2041 GTTGTGTTCC TGATCCCCCT GTGATCTCGG AAGAGAAAGG GCCCTGTTAC CGACTTGTCA 
     2101 GTTCTGGAAG ACAGTGTATG CACCCTCTGT CTGTTCACCT CACCAAGCAG CTCTGCTGTT 
     2161 GTAGTGTGGG CAAGGCCTGG GGCCCACACT GTGAGAAATG TCCCCTTCCA GGCACAGCCA 
     2221 AGGAAGAGCC AGTGGAGGCC CTGACCTTCT CCCGGGAACA CGGGCCAGGA GTGGCGGAGC 
     2281 CAGAAGTGGC AACTGCACCC CCTGAAAAGG AAATACCTTC ATTGGATCAA GAGAAAACCA 
     2341 AACTTGAGCC TGGTCAACCC CAGCTGTCTC CAGGCATTTC CACTATTCAT CTGCATCCAC 
     2401 AGTTTCCAGT AGTGATTGAA AAAACATCAC CTCCTGTGCC TGTTGAAGTA GCTCCTGAAG 
     2461 CTTCTACGTC TAGTGCCAGC CAAGTGATTG CTCCTACTCA AGTGACAGAA ATCAATGAAT 
     2521 GTACTGTGAA CCCTGATATC TGTGGAGCAG GACACTGCAT TAACCTACCA GTGAGATATA 
     2581 CCTGTATATG CTACGAGGGC TACAGGTTCA GTGAACAACA GAGGAAATGT GTGGATATTG 
     2641 ATGAGTGTAC TCAGGTCCAA CACCTCTGCT CCCAGGGCCG CTGTGAAAAC ACCGAGGGAA 
     2701 GTTTCTTGTG CATTTGCCCA GCAGGATTTA TGGCCAGTGA GGAGGGTACT AACTGCATAG 
     2761 ATGTTGACGA ATGCCTGAGG CCGGACGTCT GTGGGGAGGG GCACTGTGTC AATACTGTGG 
     2821 GGGCCTTCCG GTGTGAATAC TGTGACAGCG GGTACCGCAT GACTCAGAGA GGCCGTTGTG 
     2881 AGGATATTGA TGAATGTTTG AATCCAAGCA CTTGTCCAGA TGAGCAGTGT GTGAATTCTC 
     2941 CTGGATCTTA CCAGTGCGTT CCCTGCACAG AAGGATTCCG AGGCTGGAAT GGACAGTGCC 
     3001 TTGATGTGGA CGAGTGCCTG GAACCAAACG TCTGCGCAAA TGGTGATTGT TCCAACCTTG 
     3061 AAGGCTCCTA CATGTGTTCA TGCCACAAAG GCTATACCCG GACTCCGGAC CACAAGCACT 
     3121 GTAGAGATAT TGATGAATGT CAGCAAGGGA ATCTATGTGT AAACGGGCAG TGCAAAAATA 
     3181 CCGAGGGCTC CTTCAGGTGC ACCTGTGGAC AGGGGTACCA GCTGTCGGCA GCTAAAGACC 
     3241 AGTGTGAAGA CATTGATGAA TGCCAGCACC GTCATCTCTG TGCTCATGGG CAGTGCAGGA 
     3301 ACACTGAGGG CTCTTTTCAA TGTGTGTGTG ACCAGGGTTA CAGAGCATCT GGGCTTGGAG 
     3361 ACCACTGTGA AGATATCAAT GAATGCTTGG AGGACAAGAG TGTTTGCCAG AGAGGAGACT 
     3421 GCATTAATAC TGCAGGGTCC TATGATTGTA CTTGTCCGGA TGGATTTCAG CTAGATGACA 
     3481 ATAAAACATG TCAAGATATT AATGAATGTG AACATCCAGG GCTCTGTGGT CCGCAAGGGG 
     3541 AGTGCCTAAA CACAGAGGGT TCTTTCCATT GTGTCTGCCA GCAGGGTTTC TCAATCTCTG 
     3601 CAGATGGCCG TACGTGTGAA GATATTGATG AATGTGTAAA CAACACTGTT TGTGACAGTC 
     3661 ACGGGTTTTG TGACAATACA GCTGGCTCCT TCCGCTGCCT CTGTTATCAG GGCTTTCAAG 
     3721 CCCCACAGGA TGGGCAAGGG TGTGTGGATG TGAATGAATG TGAACTGCTC AGTGGGGTGT 
     3781 GTGGTGAAGC CTTCTGTGAA AACGTGGAAG GGTCCTTCCT GTGCGTGTGT GCTGATGAAA 
     3841 ACCAAGAGTA CAGCCCCATG ACTGGGCAGT GCCGCTCCCG GACCTCCACA GATTTAGATG 
     3901 TAGATGTAGA TCAACCCAAA GAAGAAAAGA AAGAATGCTA CTATAATCTC AATGACGCCA 
     3961 GTCTCTGTGA TAATGTGTTG GCCCCCAATG TCACGAAACA AGAATGCTGC TGTACATCAG 
     4021 GCGCGGGATG GGGAGATAAC TGCGAAATCT TCCCCTGCCC GGTCTTGGGA ACTGCTGAGT 
     4081 TCACTGAAAT GTGTCCCAAA GGGAAAGGTT TTGTGCCTGC TGGAGAATCA TCTTCTGAAG 
     4141 CTGGTGGTGA GAACTATAAA GATGCAGATG AATGCCTACT TTTTGGACAA GAAATCTGCA 
     4201 AAAATGGTTT CTGTTTGAAC ACTCGGCCTG GGTATGAATG CTACTGTAAG CAAGGGACGT 
     4261 ACTATGATCC TGTGAAACTG CAGTGCTTTG ATATGGATGA ATGTCAAGAC CCCAGTAGTT 
     4321 GTATTGATGG CCAGTGTGTT AATACAGAGG GCTCTTACAA CTGCTTCTGT ACTCACCCCA 
     4381 TGGTCCTGGA TGCGTCAGAA AAAAGATGTA TACGACCGGC TGAGTCAAAC GAACAAATAG 
     4441 AAGAAACTGA TGTCTACCAA GATTTGTGCT GGGAACATCT GAGTGATGAA TACGTGTGTA 
     4501 GCCGGCCTCT TGTGGGCAAG CAGACAACGT ACACTGAGTG CTGCTGTCTG TATGGAGAGG 
     4561 CCTGGGGCAT GCAGTGTGCC CTCTGCCCCC TGAAGGATTC AGATGACTAT GCTCAGCTGT 
     4621 GTAACATCCC CGTGACGGGA CGCCGGCAGC CATATGGACG GGACGCCTTG GTTGACTTCA 
     4681 GTGAACAGTA TACTCCAGAA GCCGATCCCT ACTTCATCCA AGACCGTTTT CTAAATAGCT 
     4741 TTGAGGAGTT ACAGGCTGAG GAATGCGGCA TCCTCAATGG ATGTGAAAAT GGTCGCTGTG 
     4801 TGAGGGTCCA GGAAGGTTAC ACCTGCGATT GCTTTGATGG GTATCACTTG GATACGGCCA 
     4861 AGATGACCTG TGTCGATGTA AATGAATGCG ATGAGTTGAA CAACCGGATG TCTCTCTGCA 
     4921 AGAATGCCAA GTGCATTAAC ACCGATGGTT CCTACAAGTG TTTGTGTCTG CCAGGCTACG 
     4981 TGCCTTCTGA CAAGCCAAAC TACTGCACTC CGTTGAATAC CGCCTTGAAT TTAGAGAAAG 
     5041 ACAGTGACCT GGAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5101 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5161 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5221 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5281 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5341 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5401 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5461 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5521 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5581 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5641 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5701 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5761 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5821 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5881 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5941 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6001 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6061 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6121 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6181 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6241 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6301 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6361 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6421 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6481 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6541 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6601 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6661 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6721 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6781 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6841 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6901 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6961 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7021 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7081 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7141 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7201 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7261 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7321 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7381 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7441 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7501 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7561 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7621 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7681 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7741 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7801 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7861 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7921 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7981 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8041 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8101 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8161 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8221 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8281 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8341 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8401 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8461 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8521 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8581 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8641 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8701 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8761 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8821 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8881 ATTTAACAAA AATTTAACGC GAATTTT
//