LOCUS       RC228673                8874 bp    DNA     circular  SYN 
DEFINITION  TNIK (Myc-DDK-tagged)-Human TRAF2 and NCK interacting kinase (TNIK), transcript variant 3
ACCESSION   NM_001161561
VERSION     NM_001161561.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5031..5122 
                     /label="Myc-DDK"  
     misc_feature    5152..5737 
                     /label="PolyA signal"  
     misc_feature    5209..5228 
                     /label="Restriction Enzyme"  
     misc_feature    6013..6685 
                     /label="ColE1"  
     misc_feature    7115..7909 
                     /label="Kan/Neo"  
     misc_feature    7944..8246 
                     /label="SV40 ori"  
     misc_feature    8332..8809 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5013..5019 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5013 
                     /label="TNIK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGAC TCCCCGGCTC GAAGCCTGGA TGAAATAGAT CTCTCGGCTC 
     1081 TGAGGGACCC CGCAGGGATC TTTGAATTGG TGGAACTTGT TGGAAATGGA ACATACGGGC 
     1141 AAGTTTATAA GGGTCGTCAT GTCAAAACGG GCCAGCTTGC AGCCATCAAG GTTATGGATG 
     1201 TCACAGGGGA TGAAGAGGAA GAAATCAAAC AAGAAATTAA CATGTTGAAG AAATATTCTC 
     1261 ATCACCGGAA TATTGCTACA TACTATGGTG CTTTTATCAA AAAGAACCCA CCAGGCATGG 
     1321 ATGACCAACT TTGGTTGGTG ATGGAGTTTT GTGGTGCTGG CTCTGTCACC GACCTGATCA 
     1381 AGAACACAAA AGGTAACACG TTGAAAGAGG AGTGGATTGC ATACATCTGC AGGGAAATCT 
     1441 TACGGGGGCT GAGTCACCTG CACCAGCATA AAGTGATTCA TCGAGATATT AAAGGGCAAA 
     1501 ATGTCTTGCT GACTGAAAAT GCAGAAGTTA AACTAGTGGA CTTTGGAGTC AGTGCTCAGC 
     1561 TTGATCGAAC AGTGGGCAGG AGGAATACTT TCATTGGAAC TCCCTACTGG ATGGCACCAG 
     1621 AAGTTATTGC CTGTGATGAA AACCCAGATG CCACATATGA TTTCAAGAGT GACTTGTGGT 
     1681 CTTTGGGTAT CACCGCCATT GAAATGGCAG AAGGTGCTCC CCCTCTCTGT GACATGCACC 
     1741 CCATGAGAGC TCTCTTCCTC ATCCCCCGGA ACCCAGCGCC TCGGCTGAAG TCTAAGAAGT 
     1801 GGTCAAAAAA ATTCCAGTCA TTTATTGAGA GCTGCTTGGT AAAGAATCAC AGCCAGCGAC 
     1861 CAGCAACAGA ACAATTGATG AAGCATCCAT TTATACGAGA CCAACCTAAT GAGCGACAGG 
     1921 TCCGCATTCA ACTCAAGGAC CATATTGATA GAACAAAGAA GAAGCGAGGA GAAAAAGATG 
     1981 AGACAGAGTA TGAGTACAGT GGAAGTGAGG AAGAAGAGGA GGAGAATGAC TCAGGAGAGC 
     2041 CCAGCTCCAT CCTGAATCTG CCAGGGGAGT CGACGCTGCG GAGGGACTTT CTGAGGCTGC 
     2101 AGCTGGCCAA CAAGGAGCGT TCTGAGGCCC TACGGAGGCA GCAGCTGGAG CAGCAGCAGC 
     2161 GGGAGAATGA GGAGCACAAG CGGCAGCTGC TGGCCGAGCG TCAGAAGCGC ATCGAGGAGC 
     2221 AGAAAGAGCA GAGGCGGCGG CTGGAGGAGC AACAAAGGCG AGAGAAGGAG CTGCGGAAGC 
     2281 AGCAGGAGAG GGAGCAGCGC CGGCACTATG AGGAGCAGAT GCGCCGGGAG GAGGAGAGGA 
     2341 GGCGTGCGGA GCATGAACAG GAATATAAGC GCAAACAATT GGAAGAACAG AGACAAGCAG 
     2401 AAAGACTGCA GAGGCAGCTA AAGCAAGAAA GAGACTACTT AGTTTCCCTT CAGCATCAGC 
     2461 GGCAGGAGCA GAGGCCTGTG GAGAAGAAGC CACTGTACCA TTACAAAGAA GGAATGAGTC 
     2521 CTAGTGAGAA GCCAGCATGG GCCAAGGAGG TAGAAGAACG GTCAAGGCTC AACCGGCAAA 
     2581 GTTCCCCTGC CATGCCTCAC AAGGTTGCCA ACAGGATATC TGACCCCAAC CTGCCCCCAA 
     2641 GGTCGGAGTC CTTCAGCATT AGTGGAGTTC AGCCTGCTCG AACACCCCCC ATGCTCAGAC 
     2701 CAGTCGATCC CCAGATCCCA CATCTGGTAG CTGTAAAATC CCAGGGACCT GCCTTGACCG 
     2761 CCTCCCAGTC AGTGCACGAG CAGCCCACAA AGGGCCTCTC TGGGTTTCAG GAGGCTCTGA 
     2821 ACGTGACCTC CCACCGCGTG GAGATGCCAC GCCAGAACTC AGATCCCACC TCGGAAAATC 
     2881 CTCCTCTCCC CACTCGCATT GAAAAGTTTG ACCGAAGCTC TTGGTTACGA CAGGAAGAAG 
     2941 ACATTCCACC AAAGGTGCCT CAAAGAACAA CTTCTATATC CCCAGCATTA GCCAGAAAGA 
     3001 ATTCTCCTGG GAATGGTAGT GCTCTGGGAC CCAGACTAGG ATCTCAACCC ATCAGAGCAA 
     3061 GCAACCCTGA TCTCCGGAGA ACTGAGCCCA TCTTGGAGAG CCCCTTGCAG AGGACCAGCA 
     3121 GTGGCAGTTC CTCCAGCTCC AGCACCCCTA GCTCCCAGCC CAGCTCCCAA GGAGGCTCCC 
     3181 AGCCTGGATC ACAAGCAGGA TCCAGTGAAC GCACCAGAGT TCGAGCCAAC AGTAAGTCAG 
     3241 AAGGATCACC TGTGCTCCCC CATGAGCCTG CCAAGGTGAA ACCAGAAGAA TCCAGGGACA 
     3301 TTACCCGGCC CAGTCGACCA GCTAGCTACA AAAAAGCTAT AGATGAGGAT CTGACGGCAT 
     3361 TAGCCAAAGA ACTAAGAGAA CTCCGGATTG AAGAAACAAA CCGCCCAATG AAGAAGGTGA 
     3421 CTGATTACTC CTCCTCCAGT GAGGAGTCAG AAAGTAGCGA GGAAGAGGAG GAAGATGGAG 
     3481 AGAGCGAGAC CCATGATGGG ACAGTGGCTG TCAGCGACAT ACCCAGACTG ATACCAACAG 
     3541 GAGCTCCAGG CAGCAACGAG CAGTACAATG TGGGAATGGT GGGGACGCAT GGGCTGGAGA 
     3601 CCTCTCATGC GGACAGTTTC AGCGGCAGTA TTTCAAGAGA AGGAACCTTG ATGATTAGAG 
     3661 AGACGTCTGG AGAGAAGAAG CGATCTGGCC ACAGTGACAG CAATGGCTTT GCTGGCCACA 
     3721 TCAACCTCCC TGACCTGGTG CAGCAGAGCC ATTCTCCAGC TGGAACCCCG ACTGAGGGAC 
     3781 TGGGGCGCGT CTCAACCCAT TCCCAGGAGA TGGACTCTGG GACTGAATAT GGCATGGGGA 
     3841 GCAGCACCAA AGCCTCCTTC ACCCCCTTTG TGGACCCCAG AGTATACCAG ACGTCTCCCA 
     3901 CTGATGAAGA TGAAGAGGAT GAGGAATCAT CAGCCGCAGC TCTGTTTACT AGCGAACTTC 
     3961 TTAGGCAAGA ACAGGCCAAA CTCAATGAAG CAAGAAAGAT TTCGGTGGTA AATGTAAACC 
     4021 CAACCAACAT TCGGCCTCAT AGCGACACAC CAGAAATCAG AAAATACAAG AAACGATTCA 
     4081 ACTCAGAAAT ACTTTGTGCA GCTCTGTGGG GTGTAAACCT TCTGGTGGGG ACTGAAAATG 
     4141 GCCTGATGCT TTTGGACCGA AGTGGGCAAG GCAAAGTCTA TAATCTGATC AACCGGAGGC 
     4201 GATTTCAGCA GATGGATGTG CTAGAGGGAC TGAATGTCCT TGTGACAATT TCAGGAAAGA 
     4261 AGAATAAGCT ACGAGTTTAC TATCTTTCAT GGTTAAGAAA CAGAATACTA CATAATGACC 
     4321 CAGAAGTAGA AAAGAAACAA GGCTGGATCA CTGTTGGGGA CTTGGAAGGC TGTATACATT 
     4381 ATAAAGTTGT TAAATATGAA AGGATCAAAT TTTTGGTGAT TGCCTTAAAG AATGCTGTGG 
     4441 AAATATATGC TTGGGCTCCT AAACCGTATC ATAAATTCAT GGCATTTAAG TCTTTTGCAG 
     4501 ATCTCCAGCA CAAGCCTCTG CTAGTTGATC TCACGGTAGA AGAAGGTCAA AGATTAAAGG 
     4561 TTATTTTTGG TTCACACACT GGTTTCCATG TAATTGATGT TGATTCAGGA AACTCTTATG 
     4621 ATATCTACAT ACCATCTCAT ATTCAGGGCA ATATCACTCC TCATGCTATT GTCATCTTGC 
     4681 CTAAAACAGA TGGAATGGAA ATGCTTGTTT GCTATGAGGA TGAGGGGGTG TATGTAAACA 
     4741 CCTATGGCCG GATAACTAAG GATGTGGTGC TCCAATGGGG AGAAATGCCC ACGTCTGTGG 
     4801 CCTACATTCA TTCCAATCAG ATAATGGGCT GGGGCGAGAA AGCTATTGAG ATCCGGTCAG 
     4861 TGGAAACAGG ACATTTGGAT GGAGTATTTA TGCATAAGCG AGCTCAAAGG TTAAAGTTTC 
     4921 TATGTGAAAG AAATGATAAG GTATTTTTTG CATCCGTGCG ATCTGGAGGA AGTAGCCAAG 
     4981 TGTTTTTCAT GACCCTCAAC AGAAATTCCA TGATGAACTG GACGCGTACG CGGCCGCTCG 
     5041 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5101 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5161 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5221 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5281 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5341 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5401 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5461 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5521 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5581 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5641 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5701 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5761 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5821 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5881 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5941 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6001 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6061 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6121 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6181 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6241 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6301 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6361 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6421 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6481 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6541 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6601 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6661 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6721 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6781 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6841 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6901 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6961 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7021 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7081 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7141 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7201 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7261 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7321 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7381 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7441 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7501 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7561 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7621 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7681 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7741 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7801 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7861 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7921 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7981 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8041 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8101 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8161 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8221 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8281 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8341 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8401 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8461 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8521 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8581 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8641 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8701 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8761 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8821 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//