LOCUS       RC228672                8850 bp    DNA     circular  SYN 
DEFINITION  TNIK (Myc-DDK-tagged)-Human TRAF2 and NCK interacting kinase (TNIK), transcript variant 4
ACCESSION   NM_001161562
VERSION     NM_001161562.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5007..5098 
                     /label="Myc-DDK"  
     misc_feature    5128..5713 
                     /label="PolyA signal"  
     misc_feature    5185..5204 
                     /label="Restriction Enzyme"  
     misc_feature    5989..6661 
                     /label="ColE1"  
     misc_feature    7091..7885 
                     /label="Kan/Neo"  
     misc_feature    7920..8222 
                     /label="SV40 ori"  
     misc_feature    8308..8785 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4989..4995 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4989 
                     /label="TNIK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGAC TCCCCGGCTC GAAGCCTGGA TGAAATAGAT CTCTCGGCTC 
     1081 TGAGGGACCC CGCAGGGATC TTTGAATTGG TGGAACTTGT TGGAAATGGA ACATACGGGC 
     1141 AAGTTTATAA GGGTCGTCAT GTCAAAACGG GCCAGCTTGC AGCCATCAAG GTTATGGATG 
     1201 TCACAGGGGA TGAAGAGGAA GAAATCAAAC AAGAAATTAA CATGTTGAAG AAATATTCTC 
     1261 ATCACCGGAA TATTGCTACA TACTATGGTG CTTTTATCAA AAAGAACCCA CCAGGCATGG 
     1321 ATGACCAACT TTGGTTGGTG ATGGAGTTTT GTGGTGCTGG CTCTGTCACC GACCTGATCA 
     1381 AGAACACAAA AGGTAACACG TTGAAAGAGG AGTGGATTGC ATACATCTGC AGGGAAATCT 
     1441 TACGGGGGCT GAGTCACCTG CACCAGCATA AAGTGATTCA TCGAGATATT AAAGGGCAAA 
     1501 ATGTCTTGCT GACTGAAAAT GCAGAAGTTA AACTAGTGGA CTTTGGAGTC AGTGCTCAGC 
     1561 TTGATCGAAC AGTGGGCAGG AGGAATACTT TCATTGGAAC TCCCTACTGG ATGGCACCAG 
     1621 AAGTTATTGC CTGTGATGAA AACCCAGATG CCACATATGA TTTCAAGAGT GACTTGTGGT 
     1681 CTTTGGGTAT CACCGCCATT GAAATGGCAG AAGGTGCTCC CCCTCTCTGT GACATGCACC 
     1741 CCATGAGAGC TCTCTTCCTC ATCCCCCGGA ACCCAGCGCC TCGGCTGAAG TCTAAGAAGT 
     1801 GGTCAAAAAA ATTCCAGTCA TTTATTGAGA GCTGCTTGGT AAAGAATCAC AGCCAGCGAC 
     1861 CAGCAACAGA ACAATTGATG AAGCATCCAT TTATACGAGA CCAACCTAAT GAGCGACAGG 
     1921 TCCGCATTCA ACTCAAGGAC CATATTGATA GAACAAAGAA GAAGCGAGGA GAAAAAGATG 
     1981 AGACAGAGTA TGAGTACAGT GGAAGTGAGG AAGAAGAGGA GGAGAATGAC TCAGGAGAGC 
     2041 CCAGCTCCAT CCTGAATCTG CCAGGGGAGT CGACGCTGCG GAGGGACTTT CTGAGGCTGC 
     2101 AGCTGGCCAA CAAGGAGCGT TCTGAGGCCC TACGGAGGCA GCAGCTGGAG CAGCAGCAGC 
     2161 GGGAGAATGA GGAGCACAAG CGGCAGCTGC TGGCCGAGCG TCAGAAGCGC ATCGAGGAGC 
     2221 AGAAAGAGCA GAGGCGGCGG CTGGAGGAGC AACAAAGGCG AGAGAAGGAG CTGCGGAAGC 
     2281 AGCAGGAGAG GGAGCAGCGC CGGCACTATG AGGAGCAGAT GCGCCGGGAG GAGGAGAGGA 
     2341 GGCGTGCGGA GCATGAACAG GAATATAAGC GCAAACAATT GGAAGAACAG AGACAAGCAG 
     2401 AAAGACTGCA GAGGCAGCTA AAGCAAGAAA GAGACTACTT AGTTTCCCTT CAGCATCAGC 
     2461 GGCAGGAGCA GAGGCCTGTG GAGAAGAAGC CACTGTACCA TTACAAAGAA GGAATGAGTC 
     2521 CTAGTGAGAA GCCAGCATGG GCCAAGGAGG TAGAAGAACG GTCAAGGCTC AACCGGCAAA 
     2581 GTTCCCCTGC CATGCCTCAC AAGGTTGCCA ACAGGATATC TGACCCCAAC CTGCCCCCAA 
     2641 GGTCGGAGTC CTTCAGCATT AGTGGAGTTC AGCCTGCTCG AACACCCCCC ATGCTCAGAC 
     2701 CAGTCGATCC CCAGATCCCA CATCTGGTAG CTGTAAAATC CCAGGGACCT GCCTTGACCG 
     2761 CCTCCCAGTC AGTGCACGAG CAGCCCACAA AGGGCCTCTC TGGGTTTCAG GAGGCTCTGA 
     2821 ACGTGACCTC CCACCGCGTG GAGATGCCAC GCCAGAACTC AGATCCCACC TCGGAAAATC 
     2881 CTCCTCTCCC CACTCGCATT GAAAAGTTTG ACCGAAGCTC TTGGTTACGA CAGGAAGAAG 
     2941 ACATTCCACC AAAGGTGCCT CAAAGAACAA CTTCTATATC CCCAGCATTA GCCAGAAAGA 
     3001 ATTCTCCTGG GAATGGTAGT GCTCTGGGAC CCAGACTAGG ATCTCAACCC ATCAGAGCAA 
     3061 GCAACCCTGA TCTCCGGAGA ACTGAGCCCA TCTTGGAGAG CCCCTTGCAG AGGACCAGCA 
     3121 GTGGCAGTTC CTCCAGCTCC AGCACCCCTA GCTCCCAGCC CAGCTCCCAA GGAGGCTCCC 
     3181 AGCCTGGATC ACAAGCAGGA TCCAGTGAAC GCACCAGAGT TCGAGCCAAC AGTAAGTCAG 
     3241 AAGGATCACC TGTGCTCCCC CATGAGCCTG CCAAGGTGAA ACCAGAAGAA TCCAGGGACA 
     3301 TTACCCGGCC CAGTCGACCA GCTGATCTGA CGGCATTAGC CAAAGAACTA AGAGAACTCC 
     3361 GGATTGAAGA AACAAACCGC CCAATGAAGA AGGTGACTGA TTACTCCTCC TCCAGTGAGG 
     3421 AGTCAGAAAG TAGCGAGGAA GAGGAGGAAG ATGGAGAGAG CGAGACCCAT GATGGGACAG 
     3481 TGGCTGTCAG CGACATACCC AGACTGATAC CAACAGGAGC TCCAGGCAGC AACGAGCAGT 
     3541 ACAATGTGGG AATGGTGGGG ACGCATGGGC TGGAGACCTC TCATGCGGAC AGTTTCAGCG 
     3601 GCAGTATTTC AAGAGAAGGA ACCTTGATGA TTAGAGAGAC GTCTGGAGAG AAGAAGCGAT 
     3661 CTGGCCACAG TGACAGCAAT GGCTTTGCTG GCCACATCAA CCTCCCTGAC CTGGTGCAGC 
     3721 AGAGCCATTC TCCAGCTGGA ACCCCGACTG AGGGACTGGG GCGCGTCTCA ACCCATTCCC 
     3781 AGGAGATGGA CTCTGGGACT GAATATGGCA TGGGGAGCAG CACCAAAGCC TCCTTCACCC 
     3841 CCTTTGTGGA CCCCAGAGTA TACCAGACGT CTCCCACTGA TGAAGATGAA GAGGATGAGG 
     3901 AATCATCAGC CGCAGCTCTG TTTACTAGCG AACTTCTTAG GCAAGAACAG GCCAAACTCA 
     3961 ATGAAGCAAG AAAGATTTCG GTGGTAAATG TAAACCCAAC CAACATTCGG CCTCATAGCG 
     4021 ACACACCAGA AATCAGAAAA TACAAGAAAC GATTCAACTC AGAAATACTT TGTGCAGCTC 
     4081 TGTGGGGTGT AAACCTTCTG GTGGGGACTG AAAATGGCCT GATGCTTTTG GACCGAAGTG 
     4141 GGCAAGGCAA AGTCTATAAT CTGATCAACC GGAGGCGATT TCAGCAGATG GATGTGCTAG 
     4201 AGGGACTGAA TGTCCTTGTG ACAATTTCAG GAAAGAAGAA TAAGCTACGA GTTTACTATC 
     4261 TTTCATGGTT AAGAAACAGA ATACTACATA ATGACCCAGA AGTAGAAAAG AAACAAGGCT 
     4321 GGATCACTGT TGGGGACTTG GAAGGCTGTA TACATTATAA AGTTGTTAAA TATGAAAGGA 
     4381 TCAAATTTTT GGTGATTGCC TTAAAGAATG CTGTGGAAAT ATATGCTTGG GCTCCTAAAC 
     4441 CGTATCATAA ATTCATGGCA TTTAAGTCTT TTGCAGATCT CCAGCACAAG CCTCTGCTAG 
     4501 TTGATCTCAC GGTAGAAGAA GGTCAAAGAT TAAAGGTTAT TTTTGGTTCA CACACTGGTT 
     4561 TCCATGTAAT TGATGTTGAT TCAGGAAACT CTTATGATAT CTACATACCA TCTCATATTC 
     4621 AGGGCAATAT CACTCCTCAT GCTATTGTCA TCTTGCCTAA AACAGATGGA ATGGAAATGC 
     4681 TTGTTTGCTA TGAGGATGAG GGGGTGTATG TAAACACCTA TGGCCGGATA ACTAAGGATG 
     4741 TGGTGCTCCA ATGGGGAGAA ATGCCCACGT CTGTGGCCTA CATTCATTCC AATCAGATAA 
     4801 TGGGCTGGGG CGAGAAAGCT ATTGAGATCC GGTCAGTGGA AACAGGACAT TTGGATGGAG 
     4861 TATTTATGCA TAAGCGAGCT CAAAGGTTAA AGTTTCTATG TGAAAGAAAT GATAAGGTAT 
     4921 TTTTTGCATC CGTGCGATCT GGAGGAAGTA GCCAAGTGTT TTTCATGACC CTCAACAGAA 
     4981 ATTCCATGAT GAACTGGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5041 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5101 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5161 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5221 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5281 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5341 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5401 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5461 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5521 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5581 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5641 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5701 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5761 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5821 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5881 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5941 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6001 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6061 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6121 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6181 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6241 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6301 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6361 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6421 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6481 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6541 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6601 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6661 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6721 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6781 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6841 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6901 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6961 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7021 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7081 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7141 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7201 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7261 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7321 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7381 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7441 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7501 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7561 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7621 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7681 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7741 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7801 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7861 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7921 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7981 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8041 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8101 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8161 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8221 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8281 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8341 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8401 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8461 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8521 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8581 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8641 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8701 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8761 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8821 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//