LOCUS       RC228669                8781 bp    DNA     circular  SYN 
DEFINITION  LTBP1 (Myc-DDK-tagged)-Human latent transforming growth factor beta binding protein 1 (LTBP1), transcript variant 5
ACCESSION   NM_001166266
VERSION     NM_001166266.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4938..5029 
                     /label="Myc-DDK"  
     misc_feature    5059..5644 
                     /label="PolyA signal"  
     misc_feature    5116..5135 
                     /label="Restriction Enzyme"  
     misc_feature    5920..6592 
                     /label="ColE1"  
     misc_feature    7022..7816 
                     /label="Kan/Neo"  
     misc_feature    7851..8153 
                     /label="SV40 ori"  
     misc_feature    8239..8716 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4920..4926 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4920 
                     /label="LTBP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATACTAAG CTGATGTGTT TGTTGTTCTT TTTCTCCCTG CCTCCGCTCC 
     1081 TAGTGAGTAA CCACACTGGC CGCATCAAGG TGGTCTTTAC TCCGAGCATC TGTAAAGTGA 
     1141 CCTGCACCAA GGGCAGCTGT CAGAACAGCT GTGAGAAGGG GAACACCACC ACTCTCATTA 
     1201 GTGAGAATGG TCATGCTGCC GACACCCTGA CGGCCACGAA CTTCCGAGTG GTAATTTGCC 
     1261 ATCTTCCATG TATGAATGGT GGCCAGTGCA GTTCAAGGGA CAAATGTCAG TGCCCTCCAA 
     1321 ATTTCACAGG AAAACTTTGT CAGATCCCAG TCCATGGTGC CAGCGTGCCT AAACTTTATC 
     1381 AGCATTCCCA GCAGCCAGGC AAGGCGTTGG GGACGCATGT CATCCATTCA ACACATACCT 
     1441 TGCCTCTGAC CGTGACTAGC CAGCAAGGAG TCAAAGTGAA ATTTCCTCCT AACATAGTCA 
     1501 ATATCCATGT GAAACATCCT CCTGAAGCTT CCGTCCAGAT ACATCAGGTT TCAAGAATTG 
     1561 ATGGCCCAAC AGGCCAGAAG ACAAAAGAAG CTCAACCAGG CCAATCCCAA GTCTCGTACC 
     1621 AAGGGCTTCC TGTCCAGAAG ACCCAGACCA TACATTCCAC ATACTCCCAC CAGCAGGTCA 
     1681 TTCCTCACGT CTACCCCGTG GCTGCTAAGA CACAGCTTGG CCGGTGCTTC CAGGAAACCA 
     1741 TTGGGTCACA GTGTGGCAAA GCGCTCCCTG GCCTTTCAAA GCAAGAGGAC TGCTGTGGAA 
     1801 CTGTGGGTAC CTCCTGGGGC TTTAACAAAT GCCAGAAATG CCCCAAGAAA CCATCTTATC 
     1861 ATGGATACAA CCAAATGATG GAATGCCTAC CGGGTTATAA GCGGGTTAAC AACACCTTTT 
     1921 GCCAAGATAT TAATGAATGT CAGCTACAAG GTGTATGCCC TAATGGTGAG TGTTTGAATA 
     1981 CCATGGGCAG CTATCGATGT ACCTGCAAAA TAGGATTTGG GCCGGATCCT ACCTTTTCAA 
     2041 GTTGTGTTCC TGATCCCCCT GTGATCTCGG AAGAGAAAGG GCCCTGTTAC CGACTTGTCA 
     2101 GTTCTGGAAG ACAGTGTATG CACCCTCTGT CTGTTCACCT CACCAAGCAG CTCTGCTGTT 
     2161 GTAGTGTGGG CAAGGCCTGG GGCCCACACT GTGAGAAATG TCCCCTTCCA GGCACAGCCA 
     2221 AGGAAGAGCC AGTGGAGGCC CTGACCTTCT CCCGGGAACA CGGGCCAGGA GTGGCGGAGC 
     2281 CAGAAGTGGC AACTGCACCC CCTGAAAAGG AAATACCTTC ATTGGATCAA GAGAAAACCA 
     2341 AACTTGAGCC TGGTCAACCC CAGCTGTCTC CAGGCATTTC CACTATTCAT CTGCATCCAC 
     2401 AGTTTCCAGT AGTGATTGAA AAAACATCAC CTCCTGTGCC TGTTGAAGTA GCTCCTGAAG 
     2461 CTTCTACGTC TAGTGCCAGC CAAGTGATTG CTCCTACTCA AGTGACAGAA ATCAATGAAT 
     2521 GTACTGTGAA CCCTGATATC TGTGGAGCAG GACACTGCAT TAACCTACCA GTGAGATATA 
     2581 CCTGTATATG CTACGAGGGC TACAGGTTCA GTGAACAACA GAGGAAATGT GTGGATATTG 
     2641 ATGAGTGTAC TCAGGTCCAA CACCTCTGCT CCCAGGGCCG CTGTGAAAAC ACCGAGGGAA 
     2701 GTTTCTTGTG CATTTGCCCA GCAGGATTTA TGGCCAGTGA GGAGGGTACT AACTGCATAG 
     2761 ATGTTGACGA ATGCCTGAGG CCGGACGTCT GTGGGGAGGG GCACTGTGTC AATACTGTGG 
     2821 GGGCCTTCCG GTGTGAATAC TGTGACAGCG GGTACCGCAT GACTCAGAGA GGCCGTTGTG 
     2881 AGGATATTGA TGAATGTTTG AATCCAAGCA CTTGTCCAGA TGAGCAGTGT GTGAATTCTC 
     2941 CTGGATCTTA CCAGTGCGTT CCCTGCACAG AAGGATTCCG AGGCTGGAAT GGACAGTGCC 
     3001 TTGATGTGGA CGAGTGCCTG GAACCAAACG TCTGCGCAAA TGGTGATTGT TCCAACCTTG 
     3061 AAGGCTCCTA CATGTGTTCA TGCCACAAAG GCTATACCCG GACTCCGGAC CACAAGCACT 
     3121 GTAGAGATAT TGATGAATGT CAGCAAGGGA ATCTATGTGT AAACGGGCAG TGCAAAAATA 
     3181 CCGAGGGCTC CTTCAGGTGC ACCTGTGGAC AGGGGTACCA GCTGTCGGCA GCTAAAGACC 
     3241 AGTGTGAAGA CATTGATGAA TGCCAGCACC GTCATCTCTG TGCTCATGGG CAGTGCAGGA 
     3301 ACACTGAGGG CTCTTTTCAA TGTGTGTGTG ACCAGGGTTA CAGAGCATCT GGGCTTGGAG 
     3361 ACCACTGTGA AGATATCAAT GAATGCTTGG AGGACAAGAG TGTTTGCCAG AGAGGAGACT 
     3421 GCATTAATAC TGCAGGGTCC TATGATTGTA CTTGTCCGGA TGGATTTCAG CTAGATGACA 
     3481 ATAAAACATG TCAAGATATT AATGAATGTG AACATCCAGG GCTCTGTGGT CCGCAAGGGG 
     3541 AGTGCCTAAA CACAGAGGGT TCTTTCCATT GTGTCTGCCA GCAGGGTTTC TCAATCTCTG 
     3601 CAGATGGCCG TACGTGTGAA GATGTGAATG AATGTGAACT GCTCAGTGGG GTGTGTGGTG 
     3661 AAGCCTTCTG TGAAAACGTG GAAGGGTCCT TCCTGTGCGT GTGTGCTGAT GAAAACCAAG 
     3721 AGTACAGCCC CATGACTGGG CAGTGCCGCT CCCGGACCTC CACAGATTTA GATGTAGATG 
     3781 TAGATCAACC CAAAGAAGAA AAGAAAGAAT GCTACTATAA TCTCAATGAC GCCAGTCTCT 
     3841 GTGATAATGT GTTGGCCCCC AATGTCACGA AACAAGAATG CTGCTGTACA TCAGGCGCGG 
     3901 GATGGGGAGA TAACTGCGAA ATCTTCCCCT GCCCGGTCTT GGGAACTGCT GAGTTCACTG 
     3961 AAATGTGTCC CAAAGGGAAA GGTTTTGTGC CTGCTGGAGA ATCATCTTCT GAAGCTGGTG 
     4021 GTGAGAACTA TAAAGATGCA GATGAATGCC TACTTTTTGG ACAAGAAATC TGCAAAAATG 
     4081 GTTTCTGTTT GAACACTCGG CCTGGGTATG AATGCTACTG TAAGCAAGGG ACGTACTATG 
     4141 ATCCTGTGAA ACTGCAGTGC TTTGATATGG ATGAATGTCA AGACCCCAGT AGTTGTATTG 
     4201 ATGGCCAGTG TGTTAATACA GAGGGCTCTT ACAACTGCTT CTGTACTCAC CCCATGGTCC 
     4261 TGGATGCGTC AGAAAAAAGA TGTATACGAC CGGCTGAGTC AAACGAACAA ATAGAAGAAA 
     4321 CTGATGTCTA CCAAGATTTG TGCTGGGAAC ATCTGAGTGA TGAATACGTG TGTAGCCGGC 
     4381 CTCTTGTGGG CAAGCAGACA ACGTACACTG AGTGCTGCTG TCTGTATGGA GAGGCCTGGG 
     4441 GCATGCAGTG TGCCCTCTGC CCCCTGAAGG ATTCAGATGA CTATGCTCAG CTGTGTAACA 
     4501 TCCCCGTGAC GGGACGCCGG CAGCCATATG GACGGGACGC CTTGGTTGAC TTCAGTGAAC 
     4561 AGTATACTCC AGAAGCCGAT CCCTACTTCA TCCAAGACCG TTTTCTAAAT AGCTTTGAGG 
     4621 AGTTACAGGC TGAGGAATGC GGCATCCTCA ATGGATGTGA AAATGGTCGC TGTGTGAGGG 
     4681 TCCAGGAAGG TTACACCTGC GATTGCTTTG ATGGGTATCA CTTGGATACG GCCAAGATGA 
     4741 CCTGTGTCGA TGTAAATGAA TGCGATGAGT TGAACAACCG GATGTCTCTC TGCAAGAATG 
     4801 CCAAGTGCAT TAACACCGAT GGTTCCTACA AGTGTTTGTG TCTGCCAGGC TACGTGCCTT 
     4861 CTGACAAGCC AAACTACTGC ACTCCGTTGA ATACCGCCTT GAATTTAGAG AAAGACAGTG 
     4921 ACCTGGAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4981 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5041 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5101 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5161 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5221 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5281 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5341 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5401 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5461 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5521 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5581 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5641 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5701 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5761 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5821 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5881 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5941 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6001 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6061 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6121 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6181 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6241 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6301 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6361 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6421 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6481 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6541 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6601 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6661 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6721 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6781 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6841 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6901 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6961 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7021 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7081 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7141 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7201 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7261 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7321 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7381 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7441 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7501 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7561 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7621 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7681 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7741 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7801 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7861 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7921 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7981 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8041 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8101 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8161 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8221 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8281 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8341 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8401 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8461 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8521 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8581 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8641 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8701 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8761 CAAAAATTTA ACGCGAATTT T
//