LOCUS       RC228667                8772 bp    DNA     circular  SYN 
DEFINITION  TNIK (Myc-DDK-tagged)-Human TRAF2 and NCK interacting kinase (TNIK), transcript variant 6
ACCESSION   NM_001161564
VERSION     NM_001161564.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4929..5020 
                     /label="Myc-DDK"  
     misc_feature    5050..5635 
                     /label="PolyA signal"  
     misc_feature    5107..5126 
                     /label="Restriction Enzyme"  
     misc_feature    5911..6583 
                     /label="ColE1"  
     misc_feature    7013..7807 
                     /label="Kan/Neo"  
     misc_feature    7842..8144 
                     /label="SV40 ori"  
     misc_feature    8230..8707 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4911..4917 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4911 
                     /label="TNIK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGAC TCCCCGGCTC GAAGCCTGGA TGAAATAGAT CTCTCGGCTC 
     1081 TGAGGGACCC CGCAGGGATC TTTGAATTGG TGGAACTTGT TGGAAATGGA ACATACGGGC 
     1141 AAGTTTATAA GGGTCGTCAT GTCAAAACGG GCCAGCTTGC AGCCATCAAG GTTATGGATG 
     1201 TCACAGGGGA TGAAGAGGAA GAAATCAAAC AAGAAATTAA CATGTTGAAG AAATATTCTC 
     1261 ATCACCGGAA TATTGCTACA TACTATGGTG CTTTTATCAA AAAGAACCCA CCAGGCATGG 
     1321 ATGACCAACT TTGGTTGGTG ATGGAGTTTT GTGGTGCTGG CTCTGTCACC GACCTGATCA 
     1381 AGAACACAAA AGGTAACACG TTGAAAGAGG AGTGGATTGC ATACATCTGC AGGGAAATCT 
     1441 TACGGGGGCT GAGTCACCTG CACCAGCATA AAGTGATTCA TCGAGATATT AAAGGGCAAA 
     1501 ATGTCTTGCT GACTGAAAAT GCAGAAGTTA AACTAGTGGA CTTTGGAGTC AGTGCTCAGC 
     1561 TTGATCGAAC AGTGGGCAGG AGGAATACTT TCATTGGAAC TCCCTACTGG ATGGCACCAG 
     1621 AAGTTATTGC CTGTGATGAA AACCCAGATG CCACATATGA TTTCAAGAGT GACTTGTGGT 
     1681 CTTTGGGTAT CACCGCCATT GAAATGGCAG AAGGTGCTCC CCCTCTCTGT GACATGCACC 
     1741 CCATGAGAGC TCTCTTCCTC ATCCCCCGGA ACCCAGCGCC TCGGCTGAAG TCTAAGAAGT 
     1801 GGTCAAAAAA ATTCCAGTCA TTTATTGAGA GCTGCTTGGT AAAGAATCAC AGCCAGCGAC 
     1861 CAGCAACAGA ACAATTGATG AAGCATCCAT TTATACGAGA CCAACCTAAT GAGCGACAGG 
     1921 TCCGCATTCA ACTCAAGGAC CATATTGATA GAACAAAGAA GAAGCGAGGA GAAAAAGATG 
     1981 AGACAGAGTA TGAGTACAGT GGAAGTGAGG AAGAAGAGGA GGAGAATGAC TCAGGAGAGC 
     2041 CCAGCTCCAT CCTGAATCTG CCAGGGGAGT CGACGCTGCG GAGGGACTTT CTGAGGCTGC 
     2101 AGCTGGCCAA CAAGGAGCGT TCTGAGGCCC TACGGAGGCA GCAGCTGGAG CAGCAGCAGC 
     2161 GGGAGAATGA GGAGCACAAG CGGCAGCTGC TGGCCGAGCG TCAGAAGCGC ATCGAGGAGC 
     2221 AGAAAGAGCA GAGGCGGCGG CTGGAGGAGC AACAAAGGCG AGAGAAGGAG CTGCGGAAGC 
     2281 AGCAGGAGAG GGAGCAGCGC CGGCACTATG AGGAGCAGAT GCGCCGGGAG GAGGAGAGGA 
     2341 GGCGTGCGGA GCATGAACAG GAATACATCA GGCGACAGTT AGAGGAGGAG CAGAGACAGT 
     2401 TAGAGATCTT GCAGCAGCAG CTACTGCATG AACAAGCTCT ACTTCTGGAA TATAAGCGCA 
     2461 AACAATTGGA AGAACAGAGA CAAGCAGAAA GACTGCAGAG GCAGCTAAAG CAAGAAAGAG 
     2521 ACTACTTAGT TTCCCTTCAG CATCAGCGGC AGGAGCAGAG GCCTGTGGAG AAGAAGCCAC 
     2581 TGTACCATTA CAAAGAAGGA ATGAGTCCTA GTGAGAAGCC AGCATGGGCC AAGGAGATCC 
     2641 CACATCTGGT AGCTGTAAAA TCCCAGGGAC CTGCCTTGAC CGCCTCCCAG TCAGTGCACG 
     2701 AGCAGCCCAC AAAGGGCCTC TCTGGGTTTC AGGAGGCTCT GAACGTGACC TCCCACCGCG 
     2761 TGGAGATGCC ACGCCAGAAC TCAGATCCCA CCTCGGAAAA TCCTCCTCTC CCCACTCGCA 
     2821 TTGAAAAGTT TGACCGAAGC TCTTGGTTAC GACAGGAAGA AGACATTCCA CCAAAGGTGC 
     2881 CTCAAAGAAC AACTTCTATA TCCCCAGCAT TAGCCAGAAA GAATTCTCCT GGGAATGGTA 
     2941 GTGCTCTGGG ACCCAGACTA GGATCTCAAC CCATCAGAGC AAGCAACCCT GATCTCCGGA 
     3001 GAACTGAGCC CATCTTGGAG AGCCCCTTGC AGAGGACCAG CAGTGGCAGT TCCTCCAGCT 
     3061 CCAGCACCCC TAGCTCCCAG CCCAGCTCCC AAGGAGGCTC CCAGCCTGGA TCACAAGCAG 
     3121 GATCCAGTGA ACGCACCAGA GTTCGAGCCA ACAGTAAGTC AGAAGGATCA CCTGTGCTCC 
     3181 CCCATGAGCC TGCCAAGGTG AAACCAGAAG AATCCAGGGA CATTACCCGG CCCAGTCGAC 
     3241 CAGCTGATCT GACGGCATTA GCCAAAGAAC TAAGAGAACT CCGGATTGAA GAAACAAACC 
     3301 GCCCAATGAA GAAGGTGACT GATTACTCCT CCTCCAGTGA GGAGTCAGAA AGTAGCGAGG 
     3361 AAGAGGAGGA AGATGGAGAG AGCGAGACCC ATGATGGGAC AGTGGCTGTC AGCGACATAC 
     3421 CCAGACTGAT ACCAACAGGA GCTCCAGGCA GCAACGAGCA GTACAATGTG GGAATGGTGG 
     3481 GGACGCATGG GCTGGAGACC TCTCATGCGG ACAGTTTCAG CGGCAGTATT TCAAGAGAAG 
     3541 GAACCTTGAT GATTAGAGAG ACGTCTGGAG AGAAGAAGCG ATCTGGCCAC AGTGACAGCA 
     3601 ATGGCTTTGC TGGCCACATC AACCTCCCTG ACCTGGTGCA GCAGAGCCAT TCTCCAGCTG 
     3661 GAACCCCGAC TGAGGGACTG GGGCGCGTCT CAACCCATTC CCAGGAGATG GACTCTGGGA 
     3721 CTGAATATGG CATGGGGAGC AGCACCAAAG CCTCCTTCAC CCCCTTTGTG GACCCCAGAG 
     3781 TATACCAGAC GTCTCCCACT GATGAAGATG AAGAGGATGA GGAATCATCA GCCGCAGCTC 
     3841 TGTTTACTAG CGAACTTCTT AGGCAAGAAC AGGCCAAACT CAATGAAGCA AGAAAGATTT 
     3901 CGGTGGTAAA TGTAAACCCA ACCAACATTC GGCCTCATAG CGACACACCA GAAATCAGAA 
     3961 AATACAAGAA ACGATTCAAC TCAGAAATAC TTTGTGCAGC TCTGTGGGGT GTAAACCTTC 
     4021 TGGTGGGGAC TGAAAATGGC CTGATGCTTT TGGACCGAAG TGGGCAAGGC AAAGTCTATA 
     4081 ATCTGATCAA CCGGAGGCGA TTTCAGCAGA TGGATGTGCT AGAGGGACTG AATGTCCTTG 
     4141 TGACAATTTC AGGAAAGAAG AATAAGCTAC GAGTTTACTA TCTTTCATGG TTAAGAAACA 
     4201 GAATACTACA TAATGACCCA GAAGTAGAAA AGAAACAAGG CTGGATCACT GTTGGGGACT 
     4261 TGGAAGGCTG TATACATTAT AAAGTTGTTA AATATGAAAG GATCAAATTT TTGGTGATTG 
     4321 CCTTAAAGAA TGCTGTGGAA ATATATGCTT GGGCTCCTAA ACCGTATCAT AAATTCATGG 
     4381 CATTTAAGTC TTTTGCAGAT CTCCAGCACA AGCCTCTGCT AGTTGATCTC ACGGTAGAAG 
     4441 AAGGTCAAAG ATTAAAGGTT ATTTTTGGTT CACACACTGG TTTCCATGTA ATTGATGTTG 
     4501 ATTCAGGAAA CTCTTATGAT ATCTACATAC CATCTCATAT TCAGGGCAAT ATCACTCCTC 
     4561 ATGCTATTGT CATCTTGCCT AAAACAGATG GAATGGAAAT GCTTGTTTGC TATGAGGATG 
     4621 AGGGGGTGTA TGTAAACACC TATGGCCGGA TAACTAAGGA TGTGGTGCTC CAATGGGGAG 
     4681 AAATGCCCAC GTCTGTGGCC TACATTCATT CCAATCAGAT AATGGGCTGG GGCGAGAAAG 
     4741 CTATTGAGAT CCGGTCAGTG GAAACAGGAC ATTTGGATGG AGTATTTATG CATAAGCGAG 
     4801 CTCAAAGGTT AAAGTTTCTA TGTGAAAGAA ATGATAAGGT ATTTTTTGCA TCCGTGCGAT 
     4861 CTGGAGGAAG TAGCCAAGTG TTTTTCATGA CCCTCAACAG AAATTCCATG ATGAACTGGA 
     4921 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4981 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5041 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5101 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5161 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5221 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5281 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5341 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5401 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5461 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5521 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5581 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5641 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5701 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5761 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5821 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5881 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5941 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6001 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6061 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6121 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6181 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6241 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6301 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6361 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6421 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6481 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6541 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6601 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6661 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6721 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6781 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6841 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6901 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6961 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7021 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7081 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7141 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7201 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7261 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7321 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7381 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7441 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7501 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7561 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7621 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7681 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7741 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7801 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7861 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7921 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7981 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8041 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8101 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8161 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8221 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8281 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8341 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8401 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8461 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8521 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8581 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8641 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8701 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8761 AACGCGAATT TT
//