LOCUS       RC228666                8769 bp    DNA     circular  SYN 
DEFINITION  PPP1R9A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 9A (PPP1R9A), transcript variant 2
ACCESSION   NM_001166161
VERSION     NM_001166161.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4926..5017 
                     /label="Myc-DDK"  
     misc_feature    5047..5632 
                     /label="PolyA signal"  
     misc_feature    5104..5123 
                     /label="Restriction Enzyme"  
     misc_feature    5908..6580 
                     /label="ColE1"  
     misc_feature    7010..7804 
                     /label="Kan/Neo"  
     misc_feature    7839..8141 
                     /label="SV40 ori"  
     misc_feature    8227..8704 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4908..4914 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4908 
                     /label="PPP1R9A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGAAAACT GAGTCTTCAG GTGAACGAAC CACTCTCAGA AGTGCCTCTC 
     1081 CTCACAGGAA TGCATATCGA ACTGAGTTTC AGGCACTGAA AAGTACCTTT GACAAACCCA 
     1141 AGTCAGATGG GGAACAAAAA ACAAAAGAAG GTGAGGGCTC CCAGCAGAGC AGGGGGAGGA 
     1201 AATATGGCTC CAATGTCAAC AGAATTAAAA ACCTATTTAT GCAGATGGGT ATGGAACCCA 
     1261 ACGAGAATGC TGCAGTCATT GCCAAAACAA GGGGGAAAGG TGGACATTCA TCTCCTCAGA 
     1321 GAAGAATGAA GCCCAAAGAA TTTCTGGAAA AAACAGATGG CTCAGTTGTT AAGTTGGAGT 
     1381 CTTCTGTTTC TGAACGAATT AGTAGATTTG ACACTATGTA CGATGGCCCT TCATATTCCA 
     1441 AGTTCACTGA GACTCGAAAG ATGTTTGAGA GAAGTGTGCA TGAATCAGGA CAGAACAACC 
     1501 GCTATTCCCC AAAGAAAGAG AAAGCTGGAG GGAGTGAACC TCAGGATGAA TGGGGAGGTT 
     1561 CCAAGTCCAA CAGAGGCAGT ACTGATTCCT TGGACAGCCT TAGCTCCCGA ACTGAGGCTG 
     1621 TCTCCCCAAC TGTGAGTCAA CTGAGTGCAG TATTTGAGAA CACTGATTCT CCCAGTGCCA 
     1681 TCATTTCTGA GAAGGCTGAA AACAATGAAT ACTCAGTGAC TGGGCATTAT CCCTTGAATT 
     1741 TACCATCTGT TACTGTTACA AATCTTGACA CATTTGGTCA CCTGAAGGAT TCTAATTCCT 
     1801 GGCCTCCTTC AAACAAGCGA GGTGTTGATA CAGAGGATGC TCACAAGAGT AATGCAACTC 
     1861 CAGTACCAGA AGTGGCTTCT AAAAGTACCT CTCTAGCTTC GATACCTGGT GAAGAGATCC 
     1921 AGCAGAGCAA GGAACCCGAG GACTCCACAT CTAATCAACA GACTCCCGAC AGCATTGACA 
     1981 AAGATGGTCC TGAAGAACCT TGTGCTGAAA GTAAGGCAAT GCCAAAGTCC GAAATCCCTT 
     2041 CACCACAAAG CCAACTGTTA GAAGATGCTG AAGCTAATTT GGTTGGAAGG GAGGCAGCAA 
     2101 AGCAACAGAG GAAAGAACTT GCAGGTGGTG ATTTCACCTC TCCTGATGCT TCTGCATCCA 
     2161 GTTGTGGAAA AGAAGTACCT GAAGATTCAA ATAATTTTGA TGGTTCCCAT GTGTACATGC 
     2221 ACAGTGACTA TAATGTGTAT AGGGTGAGAT CCAGGTATAA TTCAGACTGG GGAGAGACAG 
     2281 GCACTGAGCA GGATGAGGAG GAAGATAGTG ATGAGAACAG TTACTATCAG CCTGATATGG 
     2341 AGTACTCGGA AATTGTTGGA TTGCCAGAAG AAGAAGAAAT CCCAGCAAAT AGGAAAATTA 
     2401 AGTTTAGTAG TGCTCCTATT AAGGTTTTCA ACACATACTC CAATGAAGAC TATGACAGGA 
     2461 GAAATGACGA AGTTGACCCT GTGGCTGCTT CAGCTGAGTA TGAACTTGAA AAACGTGTAG 
     2521 AAAAGCTGGA ACTTTTCCCA GTGGAGCTAG AGAAAGATGA GGATGGTCTT GGTATAAGTA 
     2581 TTATTGGAAT GGGTGTTGGA GCAGATGCTG GACTTGAAAA GCTGGGAATA TTCGTCAAGA 
     2641 CAGTAACAGA AGGTGGTGCT GCTCAACGGG ATGGCAGAAT ACAAGTCAAT GACCAGATTG 
     2701 TGGAAGTGGA TGGAATCAGC TTGGTGGGTG TGACACAGAA TTTTGCAGCA ACAGTTCTCA 
     2761 GAAACACCAA GGGCAACGTC AGATTTGTTA TTGGGCGGGA AAAACCAGGA CAAGTGAGCG 
     2821 AGGTTGCCCA GTTGATAAGC CAGACACTGG AACAGGAGAG GCGCCAGAGA GAGCTGCTGG 
     2881 AACAGCACTA TGCCCAGTAT GATGCCGACG ATGACGAGAC AGGAGAATAT GCCACAGATG 
     2941 AAGAAGAAGA TGAGGTAGGA CCTGTCCTTC CTGGCAGCGA CATGGCCATT GAAGTCTTTG 
     3001 AGCTGCCTGA GAATGAGGAC ATGTTTTCCC CATCAGAACT GGACACAAGC AAGCTCAGTC 
     3061 ACAAGTTCAA AGAGTTGCAA ATCAAACATG CAGTTACAGA AGCAGAGATT CAAAAATTGA 
     3121 AGACCAAGCT GCAGGCAGCA GAAAATGAGA AAGTGAGGTG GGAACTAGAA AAAACCCAAC 
     3181 TCCAACAAAA CATAGAAGAG AATAAGGAAA GAATGTTGAA GTTGGAAAGC TACTGGATTG 
     3241 AGGCCCAAAC ATTATGCCAC ACAGTGAATG AGCATCTCAA AGAGACTCAA AGCCAGTATC 
     3301 AGGCCTTGGA AAAGAAATAC AACAAGGCAA AGAAGTTGAT CAAGGATTTT CAACAAAAAG 
     3361 AGCTTGATTT CATCAAAAGA CAGGAAGCAG AAAGAAAGAA AATAGAAGAT TTGGAAAAAG 
     3421 CTCATCTTGT GGAAGTGCAA GGCCTCCAAG TGCGGATTAG AGATTTGGAA GCTGAGGTAT 
     3481 TCAGGCTACT GAAGCAAAAT GGGACTCAAG TTAACAATAA TAACAACATC TTTGAGAGAA 
     3541 GAACATCTCT TGGTGAAGTC TCTAAAGGGG ATACCATGGA GAACTTGGAT GGCAAGCAGA 
     3601 CATCTTGCCA AGATGGCCTA AGTCAAGACT TGAATGAAGC AGTCCCAGAG ACAGAGCGCC 
     3661 TGGATTCAAA AGCACTGAAA ACTCGAGCCC AGCTCTCTGT GAAGAACAGA CGCCAGAGAC 
     3721 CCTCTAGGAC AAGACTGTAT GATAGTGTTA GTTCCACAGA TGGGGAGGAC AGTCTAGAGA 
     3781 GAAAGGGTTT GAGAACGTCT TCTCCAGAAT CAGATTCTGG TGTTCCACCC CTCACCCCGG 
     3841 TGGATAGCAA TGTGCCCTTC TCGTCTGACC ACATAGCTGA ATTTCAAGAA GAACCACTGG 
     3901 ACCCAGAAAT GGGGCCTCTC TCCTCTATGT GGGGAGACAC TTCACTGTTT TCTACTTCAA 
     3961 AGTCTGATCA TGATGTGGAA GAATCTCCTT GCCATCACCA AACCACCAAC AAGAAAATAT 
     4021 TACGAGAAAA AGATGATGCC AAAGATCCCA AATCACTAAG GGCATCCAGT TCATTGGCGG 
     4081 TGCAAGGAGG AAAAATTAAG CGGAAGTTTG TGGATCTGGG GGCGCCTTTG CGAAGGAATT 
     4141 CCAGCAAGGG AAAGAAGTGG AAAGAAAAAG AAAAAGAAGC CAGTAGGTTT TCTGCAGGTA 
     4201 GCAGGATCTT CAGAGGCAGA CTGGAAAACT GGACACCCAA GCCATGTTCA ACAGCTCAGA 
     4261 CCTCCACTCG TTCCCCTTGC ATGCCTTTCT CATGGTTTAA TGACAGCCGG AAAGGATCCT 
     4321 ATTCCTTCAG GAACCTGCCT GCGCCTACAA GTTCCCTTCA GCCTTCTCCT GAGACTCTAA 
     4381 TTTCAGATAA AAAGGGGTCC AAGAATTTTA CCTTCAATGA TGACTTCAGT CCCAGCAGTA 
     4441 CCAGTTCAGC AGACCTCAGC GGCTTAGGAG CAGAACCTAA AACACCAGGG CTCTCTCAGT 
     4501 CCTTAGCACT GTCATCAGAT GAGATCCTTG ATGATGGACA GTCTCCCAAA CACAGTCAGT 
     4561 GTCAGAATCG GGCCGTTCAG GAATGGAGTG TGCAGCAGGT TTCTCACTGG TTAATGAGCC 
     4621 TAAATCTGGA GCAGTATGTA TCTGAATTCA GTGCCCAAAA CATCACTGGA GAACAGCTCC 
     4681 TGCAGTTGGA TGGAAATAAA CTTAAGGCTC TTGGAATGAC AGCATCCCAG GACCGAGCAG 
     4741 TGGTCAAAAA GAAACTCAAG GAAATGAAGA TGTCTCTAGA GAAGGCTCGG AAGGCCCAAG 
     4801 AGAAAATGGA AAAACAAAGA GAAAAGCTAA GGAGAAAGGA GCAAGAGCAA ATGCAGAGGA 
     4861 AGTCCAAAAA GACAGAAAAG ATGACGTCAA CTACAGCCGA GGGTGCTGGT GAGCAGACGC 
     4921 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4981 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5041 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5101 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5161 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5221 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5281 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5341 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5401 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5461 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5521 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5581 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5641 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5701 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5761 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5821 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5881 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5941 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6001 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6061 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6121 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6181 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6241 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6301 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6361 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6421 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6481 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6541 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6601 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6661 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6721 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6781 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6841 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6901 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6961 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7021 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7081 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7141 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7201 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7261 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7321 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7381 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7441 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7501 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7561 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7621 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7681 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7741 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7801 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7861 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7921 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7981 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8041 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8221 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8281 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8341 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8401 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8461 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8521 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8581 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8641 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8701 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8761 GCGAATTTT
//