LOCUS       RC228663                8709 bp    DNA     circular  SYN 
DEFINITION  TNIK (Myc-DDK-tagged)-Human TRAF2 and NCK interacting kinase (TNIK), transcript variant 7
ACCESSION   NM_001161565
VERSION     NM_001161565.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4866..4957 
                     /label="Myc-DDK"  
     misc_feature    4987..5572 
                     /label="PolyA signal"  
     misc_feature    5044..5063 
                     /label="Restriction Enzyme"  
     misc_feature    5848..6520 
                     /label="ColE1"  
     misc_feature    6950..7744 
                     /label="Kan/Neo"  
     misc_feature    7779..8081 
                     /label="SV40 ori"  
     misc_feature    8167..8644 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4848..4854 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4848 
                     /label="TNIK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGAC TCCCCGGCTC GAAGCCTGGA TGAAATAGAT CTCTCGGCTC 
     1081 TGAGGGACCC CGCAGGGATC TTTGAATTGG TGGAACTTGT TGGAAATGGA ACATACGGGC 
     1141 AAGTTTATAA GGGTCGTCAT GTCAAAACGG GCCAGCTTGC AGCCATCAAG GTTATGGATG 
     1201 TCACAGGGGA TGAAGAGGAA GAAATCAAAC AAGAAATTAA CATGTTGAAG AAATATTCTC 
     1261 ATCACCGGAA TATTGCTACA TACTATGGTG CTTTTATCAA AAAGAACCCA CCAGGCATGG 
     1321 ATGACCAACT TTGGTTGGTG ATGGAGTTTT GTGGTGCTGG CTCTGTCACC GACCTGATCA 
     1381 AGAACACAAA AGGTAACACG TTGAAAGAGG AGTGGATTGC ATACATCTGC AGGGAAATCT 
     1441 TACGGGGGCT GAGTCACCTG CACCAGCATA AAGTGATTCA TCGAGATATT AAAGGGCAAA 
     1501 ATGTCTTGCT GACTGAAAAT GCAGAAGTTA AACTAGTGGA CTTTGGAGTC AGTGCTCAGC 
     1561 TTGATCGAAC AGTGGGCAGG AGGAATACTT TCATTGGAAC TCCCTACTGG ATGGCACCAG 
     1621 AAGTTATTGC CTGTGATGAA AACCCAGATG CCACATATGA TTTCAAGAGT GACTTGTGGT 
     1681 CTTTGGGTAT CACCGCCATT GAAATGGCAG AAGGTGCTCC CCCTCTCTGT GACATGCACC 
     1741 CCATGAGAGC TCTCTTCCTC ATCCCCCGGA ACCCAGCGCC TCGGCTGAAG TCTAAGAAGT 
     1801 GGTCAAAAAA ATTCCAGTCA TTTATTGAGA GCTGCTTGGT AAAGAATCAC AGCCAGCGAC 
     1861 CAGCAACAGA ACAATTGATG AAGCATCCAT TTATACGAGA CCAACCTAAT GAGCGACAGG 
     1921 TCCGCATTCA ACTCAAGGAC CATATTGATA GAACAAAGAA GAAGCGAGGA GAAAAAGATG 
     1981 AGACAGAGTA TGAGTACAGT GGAAGTGAGG AAGAAGAGGA GGAGAATGAC TCAGGAGAGC 
     2041 CCAGCTCCAT CCTGAATCTG CCAGGGGAGT CGACGCTGCG GAGGGACTTT CTGAGGCTGC 
     2101 AGCTGGCCAA CAAGGAGCGT TCTGAGGCCC TACGGAGGCA GCAGCTGGAG CAGCAGCAGC 
     2161 GGGAGAATGA GGAGCACAAG CGGCAGCTGC TGGCCGAGCG TCAGAAGCGC ATCGAGGAGC 
     2221 AGAAAGAGCA GAGGCGGCGG CTGGAGGAGC AACAAAGGCG AGAGAAGGAG CTGCGGAAGC 
     2281 AGCAGGAGAG GGAGCAGCGC CGGCACTATG AGGAGCAGAT GCGCCGGGAG GAGGAGAGGA 
     2341 GGCGTGCGGA GCATGAACAG GAATATAAGC GCAAACAATT GGAAGAACAG AGACAAGCAG 
     2401 AAAGACTGCA GAGGCAGCTA AAGCAAGAAA GAGACTACTT AGTTTCCCTT CAGCATCAGC 
     2461 GGCAGGAGCA GAGGCCTGTG GAGAAGAAGC CACTGTACCA TTACAAAGAA GGAATGAGTC 
     2521 CTAGTGAGAA GCCAGCATGG GCCAAGGAGA TCCCACATCT GGTAGCTGTA AAATCCCAGG 
     2581 GACCTGCCTT GACCGCCTCC CAGTCAGTGC ACGAGCAGCC CACAAAGGGC CTCTCTGGGT 
     2641 TTCAGGAGGC TCTGAACGTG ACCTCCCACC GCGTGGAGAT GCCACGCCAG AACTCAGATC 
     2701 CCACCTCGGA AAATCCTCCT CTCCCCACTC GCATTGAAAA GTTTGACCGA AGCTCTTGGT 
     2761 TACGACAGGA AGAAGACATT CCACCAAAGG TGCCTCAAAG AACAACTTCT ATATCCCCAG 
     2821 CATTAGCCAG AAAGAATTCT CCTGGGAATG GTAGTGCTCT GGGACCCAGA CTAGGATCTC 
     2881 AACCCATCAG AGCAAGCAAC CCTGATCTCC GGAGAACTGA GCCCATCTTG GAGAGCCCCT 
     2941 TGCAGAGGAC CAGCAGTGGC AGTTCCTCCA GCTCCAGCAC CCCTAGCTCC CAGCCCAGCT 
     3001 CCCAAGGAGG CTCCCAGCCT GGATCACAAG CAGGATCCAG TGAACGCACC AGAGTTCGAG 
     3061 CCAACAGTAA GTCAGAAGGA TCACCTGTGC TCCCCCATGA GCCTGCCAAG GTGAAACCAG 
     3121 AAGAATCCAG GGACATTACC CGGCCCAGTC GACCAGCTAG CTACAAAAAA GCTATAGATG 
     3181 AGGATCTGAC GGCATTAGCC AAAGAACTAA GAGAACTCCG GATTGAAGAA ACAAACCGCC 
     3241 CAATGAAGAA GGTGACTGAT TACTCCTCCT CCAGTGAGGA GTCAGAAAGT AGCGAGGAAG 
     3301 AGGAGGAAGA TGGAGAGAGC GAGACCCATG ATGGGACAGT GGCTGTCAGC GACATACCCA 
     3361 GACTGATACC AACAGGAGCT CCAGGCAGCA ACGAGCAGTA CAATGTGGGA ATGGTGGGGA 
     3421 CGCATGGGCT GGAGACCTCT CATGCGGACA GTTTCAGCGG CAGTATTTCA AGAGAAGGAA 
     3481 CCTTGATGAT TAGAGAGACG TCTGGAGAGA AGAAGCGATC TGGCCACAGT GACAGCAATG 
     3541 GCTTTGCTGG CCACATCAAC CTCCCTGACC TGGTGCAGCA GAGCCATTCT CCAGCTGGAA 
     3601 CCCCGACTGA GGGACTGGGG CGCGTCTCAA CCCATTCCCA GGAGATGGAC TCTGGGACTG 
     3661 AATATGGCAT GGGGAGCAGC ACCAAAGCCT CCTTCACCCC CTTTGTGGAC CCCAGAGTAT 
     3721 ACCAGACGTC TCCCACTGAT GAAGATGAAG AGGATGAGGA ATCATCAGCC GCAGCTCTGT 
     3781 TTACTAGCGA ACTTCTTAGG CAAGAACAGG CCAAACTCAA TGAAGCAAGA AAGATTTCGG 
     3841 TGGTAAATGT AAACCCAACC AACATTCGGC CTCATAGCGA CACACCAGAA ATCAGAAAAT 
     3901 ACAAGAAACG ATTCAACTCA GAAATACTTT GTGCAGCTCT GTGGGGTGTA AACCTTCTGG 
     3961 TGGGGACTGA AAATGGCCTG ATGCTTTTGG ACCGAAGTGG GCAAGGCAAA GTCTATAATC 
     4021 TGATCAACCG GAGGCGATTT CAGCAGATGG ATGTGCTAGA GGGACTGAAT GTCCTTGTGA 
     4081 CAATTTCAGG AAAGAAGAAT AAGCTACGAG TTTACTATCT TTCATGGTTA AGAAACAGAA 
     4141 TACTACATAA TGACCCAGAA GTAGAAAAGA AACAAGGCTG GATCACTGTT GGGGACTTGG 
     4201 AAGGCTGTAT ACATTATAAA GTTGTTAAAT ATGAAAGGAT CAAATTTTTG GTGATTGCCT 
     4261 TAAAGAATGC TGTGGAAATA TATGCTTGGG CTCCTAAACC GTATCATAAA TTCATGGCAT 
     4321 TTAAGTCTTT TGCAGATCTC CAGCACAAGC CTCTGCTAGT TGATCTCACG GTAGAAGAAG 
     4381 GTCAAAGATT AAAGGTTATT TTTGGTTCAC ACACTGGTTT CCATGTAATT GATGTTGATT 
     4441 CAGGAAACTC TTATGATATC TACATACCAT CTCATATTCA GGGCAATATC ACTCCTCATG 
     4501 CTATTGTCAT CTTGCCTAAA ACAGATGGAA TGGAAATGCT TGTTTGCTAT GAGGATGAGG 
     4561 GGGTGTATGT AAACACCTAT GGCCGGATAA CTAAGGATGT GGTGCTCCAA TGGGGAGAAA 
     4621 TGCCCACGTC TGTGGCCTAC ATTCATTCCA ATCAGATAAT GGGCTGGGGC GAGAAAGCTA 
     4681 TTGAGATCCG GTCAGTGGAA ACAGGACATT TGGATGGAGT ATTTATGCAT AAGCGAGCTC 
     4741 AAAGGTTAAA GTTTCTATGT GAAAGAAATG ATAAGGTATT TTTTGCATCC GTGCGATCTG 
     4801 GAGGAAGTAG CCAAGTGTTT TTCATGACCC TCAACAGAAA TTCCATGATG AACTGGACGC 
     4861 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4921 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4981 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5041 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5101 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5161 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5221 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5281 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5341 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5401 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5461 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5521 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5581 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5641 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5701 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5761 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5821 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5881 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5941 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6001 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6061 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6121 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6181 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6241 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6301 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6361 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6421 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6481 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6541 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6601 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6661 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6721 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6781 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6841 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6901 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6961 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7021 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7081 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7141 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7201 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7261 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7321 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7381 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7441 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7501 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7561 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7621 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7681 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7741 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7801 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7861 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7921 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7981 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8041 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8101 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8161 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8221 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8281 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8341 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8401 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8461 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8521 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8581 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8641 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8701 GCGAATTTT
//