LOCUS       RC228662                8685 bp    DNA     circular  SYN 
DEFINITION  TNIK (Myc-DDK-tagged)-Human TRAF2 and NCK interacting kinase (TNIK), transcript variant 8
ACCESSION   NM_001161566
VERSION     NM_001161566.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4842..4933 
                     /label="Myc-DDK"  
     misc_feature    4963..5548 
                     /label="PolyA signal"  
     misc_feature    5020..5039 
                     /label="Restriction Enzyme"  
     misc_feature    5824..6496 
                     /label="ColE1"  
     misc_feature    6926..7720 
                     /label="Kan/Neo"  
     misc_feature    7755..8057 
                     /label="SV40 ori"  
     misc_feature    8143..8620 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4824..4830 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4824 
                     /label="TNIK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGAC TCCCCGGCTC GAAGCCTGGA TGAAATAGAT CTCTCGGCTC 
     1081 TGAGGGACCC CGCAGGGATC TTTGAATTGG TGGAACTTGT TGGAAATGGA ACATACGGGC 
     1141 AAGTTTATAA GGGTCGTCAT GTCAAAACGG GCCAGCTTGC AGCCATCAAG GTTATGGATG 
     1201 TCACAGGGGA TGAAGAGGAA GAAATCAAAC AAGAAATTAA CATGTTGAAG AAATATTCTC 
     1261 ATCACCGGAA TATTGCTACA TACTATGGTG CTTTTATCAA AAAGAACCCA CCAGGCATGG 
     1321 ATGACCAACT TTGGTTGGTG ATGGAGTTTT GTGGTGCTGG CTCTGTCACC GACCTGATCA 
     1381 AGAACACAAA AGGTAACACG TTGAAAGAGG AGTGGATTGC ATACATCTGC AGGGAAATCT 
     1441 TACGGGGGCT GAGTCACCTG CACCAGCATA AAGTGATTCA TCGAGATATT AAAGGGCAAA 
     1501 ATGTCTTGCT GACTGAAAAT GCAGAAGTTA AACTAGTGGA CTTTGGAGTC AGTGCTCAGC 
     1561 TTGATCGAAC AGTGGGCAGG AGGAATACTT TCATTGGAAC TCCCTACTGG ATGGCACCAG 
     1621 AAGTTATTGC CTGTGATGAA AACCCAGATG CCACATATGA TTTCAAGAGT GACTTGTGGT 
     1681 CTTTGGGTAT CACCGCCATT GAAATGGCAG AAGGTGCTCC CCCTCTCTGT GACATGCACC 
     1741 CCATGAGAGC TCTCTTCCTC ATCCCCCGGA ACCCAGCGCC TCGGCTGAAG TCTAAGAAGT 
     1801 GGTCAAAAAA ATTCCAGTCA TTTATTGAGA GCTGCTTGGT AAAGAATCAC AGCCAGCGAC 
     1861 CAGCAACAGA ACAATTGATG AAGCATCCAT TTATACGAGA CCAACCTAAT GAGCGACAGG 
     1921 TCCGCATTCA ACTCAAGGAC CATATTGATA GAACAAAGAA GAAGCGAGGA GAAAAAGATG 
     1981 AGACAGAGTA TGAGTACAGT GGAAGTGAGG AAGAAGAGGA GGAGAATGAC TCAGGAGAGC 
     2041 CCAGCTCCAT CCTGAATCTG CCAGGGGAGT CGACGCTGCG GAGGGACTTT CTGAGGCTGC 
     2101 AGCTGGCCAA CAAGGAGCGT TCTGAGGCCC TACGGAGGCA GCAGCTGGAG CAGCAGCAGC 
     2161 GGGAGAATGA GGAGCACAAG CGGCAGCTGC TGGCCGAGCG TCAGAAGCGC ATCGAGGAGC 
     2221 AGAAAGAGCA GAGGCGGCGG CTGGAGGAGC AACAAAGGCG AGAGAAGGAG CTGCGGAAGC 
     2281 AGCAGGAGAG GGAGCAGCGC CGGCACTATG AGGAGCAGAT GCGCCGGGAG GAGGAGAGGA 
     2341 GGCGTGCGGA GCATGAACAG GAATATAAGC GCAAACAATT GGAAGAACAG AGACAAGCAG 
     2401 AAAGACTGCA GAGGCAGCTA AAGCAAGAAA GAGACTACTT AGTTTCCCTT CAGCATCAGC 
     2461 GGCAGGAGCA GAGGCCTGTG GAGAAGAAGC CACTGTACCA TTACAAAGAA GGAATGAGTC 
     2521 CTAGTGAGAA GCCAGCATGG GCCAAGGAGA TCCCACATCT GGTAGCTGTA AAATCCCAGG 
     2581 GACCTGCCTT GACCGCCTCC CAGTCAGTGC ACGAGCAGCC CACAAAGGGC CTCTCTGGGT 
     2641 TTCAGGAGGC TCTGAACGTG ACCTCCCACC GCGTGGAGAT GCCACGCCAG AACTCAGATC 
     2701 CCACCTCGGA AAATCCTCCT CTCCCCACTC GCATTGAAAA GTTTGACCGA AGCTCTTGGT 
     2761 TACGACAGGA AGAAGACATT CCACCAAAGG TGCCTCAAAG AACAACTTCT ATATCCCCAG 
     2821 CATTAGCCAG AAAGAATTCT CCTGGGAATG GTAGTGCTCT GGGACCCAGA CTAGGATCTC 
     2881 AACCCATCAG AGCAAGCAAC CCTGATCTCC GGAGAACTGA GCCCATCTTG GAGAGCCCCT 
     2941 TGCAGAGGAC CAGCAGTGGC AGTTCCTCCA GCTCCAGCAC CCCTAGCTCC CAGCCCAGCT 
     3001 CCCAAGGAGG CTCCCAGCCT GGATCACAAG CAGGATCCAG TGAACGCACC AGAGTTCGAG 
     3061 CCAACAGTAA GTCAGAAGGA TCACCTGTGC TCCCCCATGA GCCTGCCAAG GTGAAACCAG 
     3121 AAGAATCCAG GGACATTACC CGGCCCAGTC GACCAGCTGA TCTGACGGCA TTAGCCAAAG 
     3181 AACTAAGAGA ACTCCGGATT GAAGAAACAA ACCGCCCAAT GAAGAAGGTG ACTGATTACT 
     3241 CCTCCTCCAG TGAGGAGTCA GAAAGTAGCG AGGAAGAGGA GGAAGATGGA GAGAGCGAGA 
     3301 CCCATGATGG GACAGTGGCT GTCAGCGACA TACCCAGACT GATACCAACA GGAGCTCCAG 
     3361 GCAGCAACGA GCAGTACAAT GTGGGAATGG TGGGGACGCA TGGGCTGGAG ACCTCTCATG 
     3421 CGGACAGTTT CAGCGGCAGT ATTTCAAGAG AAGGAACCTT GATGATTAGA GAGACGTCTG 
     3481 GAGAGAAGAA GCGATCTGGC CACAGTGACA GCAATGGCTT TGCTGGCCAC ATCAACCTCC 
     3541 CTGACCTGGT GCAGCAGAGC CATTCTCCAG CTGGAACCCC GACTGAGGGA CTGGGGCGCG 
     3601 TCTCAACCCA TTCCCAGGAG ATGGACTCTG GGACTGAATA TGGCATGGGG AGCAGCACCA 
     3661 AAGCCTCCTT CACCCCCTTT GTGGACCCCA GAGTATACCA GACGTCTCCC ACTGATGAAG 
     3721 ATGAAGAGGA TGAGGAATCA TCAGCCGCAG CTCTGTTTAC TAGCGAACTT CTTAGGCAAG 
     3781 AACAGGCCAA ACTCAATGAA GCAAGAAAGA TTTCGGTGGT AAATGTAAAC CCAACCAACA 
     3841 TTCGGCCTCA TAGCGACACA CCAGAAATCA GAAAATACAA GAAACGATTC AACTCAGAAA 
     3901 TACTTTGTGC AGCTCTGTGG GGTGTAAACC TTCTGGTGGG GACTGAAAAT GGCCTGATGC 
     3961 TTTTGGACCG AAGTGGGCAA GGCAAAGTCT ATAATCTGAT CAACCGGAGG CGATTTCAGC 
     4021 AGATGGATGT GCTAGAGGGA CTGAATGTCC TTGTGACAAT TTCAGGAAAG AAGAATAAGC 
     4081 TACGAGTTTA CTATCTTTCA TGGTTAAGAA ACAGAATACT ACATAATGAC CCAGAAGTAG 
     4141 AAAAGAAACA AGGCTGGATC ACTGTTGGGG ACTTGGAAGG CTGTATACAT TATAAAGTTG 
     4201 TTAAATATGA AAGGATCAAA TTTTTGGTGA TTGCCTTAAA GAATGCTGTG GAAATATATG 
     4261 CTTGGGCTCC TAAACCGTAT CATAAATTCA TGGCATTTAA GTCTTTTGCA GATCTCCAGC 
     4321 ACAAGCCTCT GCTAGTTGAT CTCACGGTAG AAGAAGGTCA AAGATTAAAG GTTATTTTTG 
     4381 GTTCACACAC TGGTTTCCAT GTAATTGATG TTGATTCAGG AAACTCTTAT GATATCTACA 
     4441 TACCATCTCA TATTCAGGGC AATATCACTC CTCATGCTAT TGTCATCTTG CCTAAAACAG 
     4501 ATGGAATGGA AATGCTTGTT TGCTATGAGG ATGAGGGGGT GTATGTAAAC ACCTATGGCC 
     4561 GGATAACTAA GGATGTGGTG CTCCAATGGG GAGAAATGCC CACGTCTGTG GCCTACATTC 
     4621 ATTCCAATCA GATAATGGGC TGGGGCGAGA AAGCTATTGA GATCCGGTCA GTGGAAACAG 
     4681 GACATTTGGA TGGAGTATTT ATGCATAAGC GAGCTCAAAG GTTAAAGTTT CTATGTGAAA 
     4741 GAAATGATAA GGTATTTTTT GCATCCGTGC GATCTGGAGG AAGTAGCCAA GTGTTTTTCA 
     4801 TGACCCTCAA CAGAAATTCC ATGATGAACT GGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4861 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4921 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4981 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5041 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5101 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5161 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5221 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5281 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5341 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5401 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5461 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5521 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5581 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5641 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5701 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5761 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5821 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5881 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5941 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6001 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6061 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6121 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6181 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6241 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6301 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6361 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6421 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6481 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6541 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6601 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6661 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6721 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6781 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6841 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6901 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6961 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7021 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7081 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7141 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7201 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7261 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7321 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7381 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7441 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7501 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7561 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7621 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7681 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7741 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7801 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7861 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7921 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8041 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8101 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8161 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8221 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8281 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8341 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8401 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8461 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8521 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8581 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8641 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//