LOCUS       RC228658                8652 bp    DNA     circular  SYN 
DEFINITION  ABCB5 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 5 (ABCB5), transcript variant 1
ACCESSION   NM_001163941
VERSION     NM_001163941.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4809..4900 
                     /label="Myc-DDK"  
     misc_feature    4930..5515 
                     /label="PolyA signal"  
     misc_feature    4987..5006 
                     /label="Restriction Enzyme"  
     misc_feature    5791..6463 
                     /label="ColE1"  
     misc_feature    6893..7687 
                     /label="Kan/Neo"  
     misc_feature    7722..8024 
                     /label="SV40 ori"  
     misc_feature    8110..8587 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4791..4797 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4791 
                     /label="ABCB5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAAATTCA GAAAGAGCTG AAGAAATGCA AGAAAATTAT CAGAGAAATG 
     1081 GAACTGCAGA AGAACAGCCA AAACTGAGAA AGGAAGCAGT TGGATCTATT GAGATATTCC 
     1141 GCTTTGCTGA TGGACTGGAC ATCACACTCA TGATCCTGGG TATACTGGCA TCACTGGTCA 
     1201 ATGGAGCCTG CCTTCCTTTA ATGCCACTGG TTTTAGGAGA AATGAGTGAT AACCTTATTA 
     1261 GTGGATGTCT AGTCCAAACT AACACAACAA ATTATCAGAA CTGTACTCAG TCTCAAGAGA 
     1321 AGCTGAATGA AGATATGACT CTGTTGACCC TGTATTATGT TGGAATAGGT GTTGCTGCCT 
     1381 TGATTTTTGG TTACATACAG ATTTCCTTGT GGATTATAAC TGCAGCACGA CAGACCAAGA 
     1441 GGATTCGAAA ACAGTTTTTT CATTCAGTTT TGGCACAGGA CATCGGCTGG TTTGATAGCT 
     1501 GTGACATCGG TGAACTTAAC ACTCGCATGA CAGATGACAT TGACAAAATC AGTGATGGTA 
     1561 TTGGAGATAA GATTGCTCTG TTGTTTCAAA ACATGTCTAC TTTTTCGATT GGCCTGGCAG 
     1621 TTGGTTTGGT GAAGGGCTGG AAACTCACCC TAGTGACTCT ATCCACGTCT CCTCTTATAA 
     1681 TGGCTTCAGC GGCAGCATGT TCTAGGATGG TCATCTCATT GACCAGTAAG GAATTAAGTG 
     1741 CCTATTCCAA AGCTGGGGCT GTGGCAGAAG AAGTCTTGTC ATCAATCCGA ACAGTCATAG 
     1801 CCTTTAGGGC CCAGGAGAAA GAACTTCAAA GGTATACACA GAATCTCAAA GATGCAAAGG 
     1861 ATTTTGGCAT AAAAAGGACT ATAGCTTCAA AAGTGTCTCT TGGTGCTGTG TACTTCTTTA 
     1921 TGAATGGAAC CTATGGACTT GCTTTTTGGT ATGGAACCTC CTTGATTCTT AATGGAGAAC 
     1981 CTGGATATAC CATCGGGACT GTTCTTGCTG TTTTCTTTAG TGTAATCCAT AGCAGTTATT 
     2041 GCATTGGAGC AGCAGTCCCT CACTTTGAAA CCTTCGCAAT AGCCCGAGGA GCTGCCTTTC 
     2101 ATATTTTCCA GGTTATTGAT AAGAAACCCA GTATAGATAA CTTTTCCACA GCTGGATATA 
     2161 AACCTGAATC CATAGAAGGA ACTGTGGAAT TTAAAAATGT TTCTTTCAAT TATCCATCAA 
     2221 GACCATCTAT CAAGATTCTG AAAGGTCTGA ATCTCAGAAT TAAGTCTGGA GAGACAGTCG 
     2281 CCTTGGTCGG TCTCAATGGC AGTGGGAAGA GTACGGTAGT CCAGCTTCTG CAGAGGTTAT 
     2341 ATGATCCGGA TGATGGCTTT ATCATGGTGG ATGAGAATGA CATCAGAGCT TTAAATGTGC 
     2401 GGCATTATCG AGACCATATT GGAGTGGTTA GTCAAGAGCC TGTTTTGTTC GGGACCACCA 
     2461 TCAGTAACAA TATCAAGTAT GGACGAGATG ATGTGACTGA TGAAGAGATG GAGAGAGCAG 
     2521 CAAGGGAAGC AAATGCGTAT GATTTTATCA TGGAGTTTCC TAATAAATTT AATACATTGG 
     2581 TAGGGGAAAA AGGAGCTCAA ATGAGTGGAG GGCAGAAACA GAGGATCGCA ATTGCTCGTG 
     2641 CCTTAGTTCG AAACCCCAAG ATTCTGATTT TAGATGAGGC TACGTCTGCC CTGGATTCAG 
     2701 AAAGCAAGTC AGCTGTTCAA GCTGCACTGG AGAAGGCGAG CAAAGGTCGG ACTACAATCG 
     2761 TGGTAGCACA CCGACTTTCT ACTATTCGAA GTGCAGATTT GATTGTGACC CTAAAGGATG 
     2821 GAATGCTGGC GGAGAAAGGA GCACATGCTG AACTAATGGC AAAACGAGGT CTATATTATT 
     2881 CACTTGTGAT GTCACAGGAT ATTAAAAAAG CTGATGAACA GATGGAGTCA ATGACATATT 
     2941 CTACTGAAAG AAAGACCAAC TCACTTCCTC TGCACTCTGT GAAGAGCATC AAGTCAGACT 
     3001 TCATTGACAA GGCTGAGGAA TCCACCCAAT CTAAAGAGAT AAGTCTTCCT GAAGTCTCTC 
     3061 TATTAAAAAT TTTAAAGTTA AACAAGCCTG AATGGCCTTT TGTGGTTCTG GGGACATTGG 
     3121 CTTCTGTTCT AAATGGAACT GTTCATCCAG TATTTTCCAT CATCTTTGCA AAAATTATAA 
     3181 CCATGTTTGG AAATAATGAT AAAACCACAT TAAAGCATGA TGCAGAAATT TATTCCATGA 
     3241 TATTCGTCAT TTTGGGTGTT ATTTGCTTTG TCAGTTATTT CATGCAGGGA TTATTTTACG 
     3301 GCAGAGCAGG GGAAATTTTA ACGATGAGAT TAAGACACTT GGCCTTCAAA GCCATGTTAT 
     3361 ATCAGGATAT TGCCTGGTTT GATGAAAAGG AAAACAGCAC AGGAGGCTTG ACAACAATAT 
     3421 TAGCCATAGA TATAGCACAA ATTCAAGGAG CAACAGGTTC CAGGATTGGC GTCTTAACAC 
     3481 AAAATGCAAC TAACATGGGA CTTTCAGTTA TCATTTCCTT TATATATGGA TGGGAGATGA 
     3541 CATTCCTGAT TCTGAGTATT GCTCCAGTAC TTGCCGTGAC AGGAATGATT GAAACCGCAG 
     3601 CAATGACTGG ATTTGCCAAC AAAGATAAGC AAGAACTTAA GCATGCTGGA AAGATAGCAA 
     3661 CTGAAGCTTT GGAGAATATA CGTACTATAG TGTCATTAAC AAGGGAAAAA GCCTTCGAGC 
     3721 AAATGTATGA AGAGATGCTT CAGACTCAAC ACAGAAATAC CTCGAAGAAA GCACAGATTA 
     3781 TTGGAAGCTG TTATGCATTC AGCCATGCCT TTATATATTT TGCCTATGCG GCAGGGTTTC 
     3841 GATTTGGAGC CTATTTAATT CAAGCTGGAC GAATGACCCC AGAGGGCATG TTCATAGTTT 
     3901 TTACTGCAAT TGCATATGGA GCTATGGCCA TCGGAGAAAC GCTCGTTTTG GCTCCTGAAT 
     3961 ATTCCAAAGC CAAATCGGGG GCTGCGCATC TGTTTGCCTT GTTGGAAAAG AAACCAAATA 
     4021 TAGACAGCCG CAGTCAAGAA GGGAAAAAGC CAGACACATG TGAAGGGAAT TTAGAGTTTC 
     4081 GAGAAGTCTC TTTCTTCTAT CCATGTCGCC CAGATGTTTT CATCCTCCGT GGCTTATCCC 
     4141 TCAGTATTGA GCGAGGAAAG ACAGTAGCAT TTGTGGGGAG CAGCGGCTGT GGGAAAAGCA 
     4201 CTTCTGTTCA ACTTCTGCAG AGACTTTATG ACCCCGTGCA AGGACAAGTG CTGTTTGATG 
     4261 GTGTGGATGC AAAAGAATTG AATGTACAGT GGCTCCGTTC CCAAATAGCA ATCGTTCCTC 
     4321 AAGAGCCTGT GCTCTTCAAC TGCAGCATTG CTGAGAACAT CGCCTATGGT GACAACAGCC 
     4381 GTGTGGTGCC ATTAGATGAG ATCAAAGAAG CCGCAAATGC AGCAAATATC CATTCTTTTA 
     4441 TTGAAGGTCT CCCTGAGAAA TACAACACAC AAGTTGGACT GAAAGGAGCA CAGCTTTCTG 
     4501 GCGGCCAGAA ACAAAGACTA GCTATTGCAA GGGCTCTTCT CCAAAAACCC AAAATTTTAT 
     4561 TGTTGGATGA GGCCACTTCA GCCCTCGATA ATGACAGTGA GAAGGTGGTT CAGCATGCCC 
     4621 TTGATAAAGC CAGGACGGGA AGGACATGCC TAGTGGTCAC TCACAGGCTC TCTGCAATTC 
     4681 AGAACGCAGA TTTGATAGTG GTTCTGCACA ATGGAAAGAT AAAGGAACAA GGAACTCATC 
     4741 AAGAGCTCCT GAGAAATCGA GACATATATT TTAAGTTAGT GAATGCACAG TCAGTGCAGA 
     4801 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4861 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4921 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4981 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5041 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5101 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5161 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5221 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5281 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5341 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5401 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5461 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5521 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5581 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5641 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5701 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5761 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5821 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5881 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5941 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6001 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6061 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6121 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6181 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6241 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6301 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6361 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6421 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6481 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6541 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6601 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6661 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6721 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6781 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6841 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6901 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6961 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7021 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7081 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7141 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7201 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7261 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7321 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7381 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7441 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7501 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7561 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7621 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7681 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7741 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7801 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7861 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8041 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8101 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8161 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8221 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8281 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8341 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8401 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8461 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8521 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8581 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8641 AACGCGAATT TT
//