LOCUS       RC228654                8565 bp    DNA     circular  SYN 
DEFINITION  EVC2 (Myc-DDK-tagged)-Human Ellis van Creveld syndrome 2 (EVC2), transcript variant 2
ACCESSION   NM_001166136
VERSION     NM_001166136.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4722..4813 
                     /label="Myc-DDK"  
     misc_feature    4843..5428 
                     /label="PolyA signal"  
     misc_feature    4900..4919 
                     /label="Restriction Enzyme"  
     misc_feature    5704..6376 
                     /label="ColE1"  
     misc_feature    6806..7600 
                     /label="Kan/Neo"  
     misc_feature    7635..7937 
                     /label="SV40 ori"  
     misc_feature    8023..8500 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4704..4710 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4704 
                     /label="EVC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTTGGCCC AAAGTGGAAT GCTGTCACTT TAAGACTGCA GTGGAAGCAC 
     1081 CACTTGGAAT GAAATTGGAC AAGAAAATGG AAGTCTTCAT CCCACTCTCA ACTTCTGCAG 
     1141 CCTCTAGTGG GCCATGGGCT CATTCCTTAT TTGCTTTTAT ACCCTCCTGG CCTAAGAAGA 
     1201 ACTTATTTAA AAGAGAGTCT CCTATAACAC ACCGCCTGTA TGGGGACATT TCAAGAGAAG 
     1261 TTCAAGGGAC TTCTGAAAAT GGAGTAATTT TTCAGAAATG TGCACTGGTG TCTGGGTCGA 
     1321 GTGAAGCACA GACAGCCCGC ATATGGCTGC TTGTTAACAA CACCAAGACA ACCTCGTCAG 
     1381 CCAACCTCTC GGAGCTGCTC TTGCTGGACA GCATTGCTGG TCTCACCATT TGGGACTCTG 
     1441 TGGGAAACAG GACCTCGGAA GGATTCCAGG CTTTTAGCAA GAAGTTTCTG CAAGTGGGAG 
     1501 ATGCCTTTGC TGTCAGCTAC GCAGCCACGC TCCAGGCTGG AGACCTCGGG AACGGGGAGA 
     1561 GCCTCAAGCT TCCTGCCCAA CTCACCTTTC AGAGCTCGTC ACGGAACAGA ACACAGCTGA 
     1621 AAGTGCTTTT TTCCATAACA GCAGAAGAAA ACGTAACGGT TCTGCCGCAC CACGGCCTCC 
     1681 ACGCAGCAGG GTTCTTCATT GCCTTCCTCC TCTCCCTTGT GCTGACCTGG GCTGCCCTCT 
     1741 TCCTCATGGT TCGCTATCAG TGTCTGAAGG GAAACATGCT CACCAGACAT CGGGTTTGGC 
     1801 AGTATGAGAG CAAGCTGGAA CCCTTGCCGT TCACCTCAGC TGATGGCGTG AATGAGGACC 
     1861 TTTCCCTTAA CGACCAAATG ATAGACATTC TGTCTTCCGA GGACCCTGGG AGCATGCTTC 
     1921 AAGCCTTAGA AGAGTTGGAG ATTGCAACCC TGAATCGGGC AGATGCAGAT CTGGAGGCTT 
     1981 GTCGAACACA AATCAGCAAG GATATCATTG CCCTTCTGCT GAAAAATCTC ACCAGCAGTG 
     2041 GCCACCTCTC ACCCCAAGTA GAGAGAAAAA TGAGTGCTGT TTTCAAAAAG CAGTTTCTAT 
     2101 TGCTGGAAAA TGAAATACAA GAGGAGTACG ATCGGAAGAT GGTGGCATTG ACAGCTGAAT 
     2161 GTGACCTGGA AACAAGAAAG AAGATGGAAA ACCAGTACCA GAGAGAGATG ATGGCAATGG 
     2221 AGGAAGCAGA AGAGTTGCTG AAACGTGCTG GTGAGAGGTC TGCTGTAGAG TGCAGCAACC 
     2281 TTCTGCGGAC CCTCCATGGC CTGGAACAGG AGCACTTGAG GAAGTCTCTC GCTTTGCAAC 
     2341 AAGAAGAAGA CTTTGCCAAA GCTCACAGAC AGCTGGCTGT TTTCCAGAGA AATGAACTGC 
     2401 ACAGTATCTT TTTTACCCAG ATAAAAAGTG CTATTTTCAA AGGGGAATTG AAACCAGAGG 
     2461 CAGCTAAAAT GCTGCTGCAA AATTATTCTA AAATACAGGA GAATGTAGAA GAGTTAATGG 
     2521 ACTTTTTCCA GGCTAGTAAG AGGTATCATC TAAGTAAAAG ATTTGGCCAC AGGGAATATC 
     2581 TGGTCCAGAA CCTCCAGTCA TCAGAGACCC GTGTGCAGGG CCTTCTGAGC ACCGCTGCAG 
     2641 CCCAGCTGAC TCACCTCATT CAGAAGCACG AGAGAGCAGG GTACCTGGAT GAAGACCAAA 
     2701 TGGAAATGCT ATTGGAGCGG GCTCAGACAG AAGTCTTTTC AATCAAGCAG AAGTTGGACA 
     2761 ATGACTTAAA GCAGGAAAAG AAAAAGCTCC ACCAAAAATT AATAACTAAG AGAAGACGAG 
     2821 AGTTGCTACA AAAGCACAGG GAGCAGCGTA GGGAGCAGGC GTCCGTCGGC GAGGCCTTCC 
     2881 GAACGGTTGA GGATGCCGGC CAGTACCTGC ACCAGAAGAG GAGCCTGATG GAGGAGCACG 
     2941 GTGCCACCCT GGAGGAGCTG CAGGAGCGTC TGGACCAGGC CGCCCTGGAC GATCTCAGGA 
     3001 CCCTGACCCT TTCGCTGTTT GAAAAGGCCA CCGACGAGCT GCGGCGCCTG CAGAACTCAG 
     3061 CCATGACCCA GGAGCTGCTC AAGCGTGGGG TGCCCTGGCT CTTCCTGCAG CAGATCCTGG 
     3121 AGGAGCACGG CAAGGAGATG GCTGCACGGG CCGAGCAGCT GGAGGGGGAG GAGAGGGACA 
     3181 GGGACCAGGA GGGTGTCCAG AGCGTGAGGC AGAGACTGAA GGATGACGCT CCTGAGGCCG 
     3241 TGACAGAGGA GCAGGCAGAG CTGCGACGCT GGGAGCACCT GATCTTCATG AAGCTCTGCT 
     3301 CCTCAGTCTT CTCCCTGTCT GAAGAGGAGC TGCTCAGGAT GAGGCAGGAG GTCCATGGCT 
     3361 GCTTTGCTCA GATGGACAGG AGCTTGGCCC TCCCCAAGAT CCGGGCCCGA GTTCTGCTGC 
     3421 AGCAATTTCA GACTGCGTGG CGAGAAGCAG AGTTCGTGAA GCTGGACCAG GCCGTGGCTG 
     3481 CCCCTGAGCT GCAGCAACAG TCCAAGGTGA GAAAGTCACG GTCCAAGAGT AAAAGCAAGG 
     3541 GAGAGCTTCT GAAGAAGTGC ATCGAAGACA AAATTCACCT CTGTGAGGAA CAGGCCTCTG 
     3601 AAGACCTGGT GGAAAAGGTT CGAGGTGAAT TGCTGCGGGA GAGAGTGCAG CGGATGGAGG 
     3661 CACAGGAGGG AGGCTTTGCA CAGTCGCTTG TTGCTCTGCA GTTCCAGAAG GCGTCCCGGG 
     3721 TGACCGAGAC TCTGTCGGCC TACACCGCCC TCCTCAGCAT CCAGGACTTG CTCCTGGAAG 
     3781 AGCTGAGTGC ATCTGAGATG CTGACCAAGT CGGCCTGCAC ACAGATCCTG GAGTCGCACA 
     3841 GCCGGGAGCT CCAGGAGTTG GAGAGGAAGC TGGAGGACCA GCTGGTGCAG CAGGAGGCAG 
     3901 CCCAGCAGCA GCAGGCCCTG GCGAGCTGGC AGCAGTGGGT GGCCGATGGG CCCGGGATTC 
     3961 TGAACGAACC TGGGGAGGTG GATTCTGAAA GGCAGGTCTC TACTGTCCTG CACCAAGCCC 
     4021 TGAGCAAGAG CCAGACATTA CTGGAGCAAC ATCAGCAGTG TTTGAGAGAG GAACAACAGA 
     4081 ACAGTGTCGT GCTAGAAGAC TTGTTGGAAA ACATGGAGGC AGACACCTTT GCAACCCTGT 
     4141 GCAGCCAGGA GCTGAGACTG GCATCGTACC TGGCGAGGAT GGCCATGGTG CCCGGGGCCA 
     4201 CGCTTCGCCG GCTCCTGAGT GTGGTACTGC CCACAGCCTC ACAGCCTCAG CTGCTGGCCC 
     4261 TGCTGGATTC GGCCACCGAG AGACATGTGG ACCACGCAGC TGAGAGCGAT GGCGGAGCGG 
     4321 AGCAGGCCGA CGTGGGCAGG CGGAGGAAAC ACCAGAGCTG GTGGCAAGCC TTAGATGGCA 
     4381 AACTGCGAGG AGATCTGATA AGCAGAGGAT TAGAAAAGAT GCTGTGGGCC CGCAAGAGAA 
     4441 AGCAGAGCAT ATTAAAGAAG ACATGTCTCC CTCTCAGAGA GAGGATGATA TTCTCTGGAA 
     4501 AAGGAAGTTG GCCACACCTG TCACTGGAGC CCATTGGCGA ACTGGCCCCT GTACCCATTG 
     4561 TAGGGGCAGA AACCATTGAT CTATTAAACA CAGGAGAGAA GCTCTTTATA TTCAGAAATC 
     4621 CAAAGGAGCC AGAGATCTCA CTGCACGTTC CTCCCAGGAA AAAGAAGAAC TTTTTGAATG 
     4681 CCAAAAAGGC CATGAGGGCC TTGGGCATGG ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4741 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4801 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4861 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4921 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4981 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5041 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5101 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5161 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5221 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5281 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5341 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5401 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5461 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5521 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5581 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5641 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5701 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5761 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5821 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5881 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5941 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6001 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6061 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6121 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6181 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6241 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6301 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6361 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6421 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6481 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6541 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6601 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6661 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6721 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6781 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6841 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6901 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6961 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7021 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7081 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7141 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7201 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7261 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7321 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7381 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7441 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7501 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7561 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7621 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7681 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7741 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7801 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7861 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7921 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7981 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8041 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8101 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8161 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8221 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8281 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8341 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8401 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8461 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8521 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//