LOCUS       RC228653                8547 bp    DNA     circular  SYN 
DEFINITION  KCNMA1 (Myc-DDK-tagged)-Human potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant 4
ACCESSION   NM_001161353
VERSION     NM_001161353.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4702..4793 
                     /label="Myc-DDK"  
     misc_feature    4823..5408 
                     /label="PolyA signal"  
     misc_feature    4880..4899 
                     /label="Restriction Enzyme"  
     misc_feature    5684..6356 
                     /label="ColE1"  
     misc_feature    6786..7580 
                     /label="Kan/Neo"  
     misc_feature    7615..7917 
                     /label="SV40 ori"  
     misc_feature    8003..8480 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4677..4684 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4677 
                     /label="KCNMA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAATGGT GGCGGCGGCG GCGGCGGCAG CAGCGGCGGC GGCGGCGGCG 
     1081 GCGGAGGCAG CAGTCTTAGA ATGAGTAGCA ATATCCACGC GAACCATCTC AGCCTAGACG 
     1141 CGTCCTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC TTCCTCCTCC TCTTCCTCCT 
     1201 CGTCCTCGGT CCACGAGCCC AAGATGGATG CGCTCATCAT CCCGGTGACC ATGGAGGTGC 
     1261 CGTGCGACAG CCGGGGCCAA CGCATGTGGT GGGCTTTCCT GGCCTCCTCC ATGGTGACTT 
     1321 TCTTCGGGGG CCTCTTCATC ATCTTGCTCT GGCGGACGCT CAAGTACCTG TGGACCGTGT 
     1381 GCTGCCACTG CGGGGGCAAG ACGAAGGAGG CCCAGAAGAT TAACAATGGC TCAAGCCAGG 
     1441 CGGATGGCAC TCTCAAACCA GTGGATGAAA AAGAGGAGGC AGTGGCCGCC GAGGTCGGCT 
     1501 GGATGACCTC CGTGAAGGAC TGGGCGGGGG TGATGATATC CGCCCAGACA CTGACTGGCA 
     1561 GAGTCCTGGT TGTCTTAGTC TTTGCTCTCA GCATCGGTGC ACTTGTAATA TACTTCATAG 
     1621 ATTCATCAAA CCCAATAGAA TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATCG 
     1681 ACATGGCTTT CAACGTGTTC TTCCTTCTCT ACTTCGGCTT GCGGTTTATT GCAGCCAACG 
     1741 ATAAATTGTG GTTCTGGCTG GAAGTGAACT CTGTAGTGGA TTTCTTCACG GTGCCCCCCG 
     1801 TGTTTGTGTC TGTGTACTTA AACAGAAGTT GGCTTGGTTT GAGATTTTTA AGAGCTCTGA 
     1861 GACTGATACA GTTTTCAGAA ATTTTGCAGT TTCTGAATAT TCTTAAAACA AGTAATTCCA 
     1921 TCAAGCTGGT GAATCTGCTC TCCATATTTA TCAGCACGTG GCTGACTGCA GCCGGGTTCA 
     1981 TCCATTTGGT GGAGAATTCA GGGGACCCAT GGGAAAATTT CCAAAACAAC CAGGCTCTCA 
     2041 CCTACTGGGA ATGTGTCTAT TTACTCATGG TCACAATGTC CACCGTTGGT TATGGGGATG 
     2101 TTTATGCAAA AACCACACTT GGGCGCCTCT TCATGGTCTT CTTCATCCTC GGGGGACTGG 
     2161 CCATGTTTGC CAGCTACGTC CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATACG 
     2221 GGGGCTCCTA TAGTGCGGTT AGTGGAAGAA AGCACATTGT GGTCTGCGGA CACATCACTC 
     2281 TGGAGAGTGT TTCCAACTTC CTGAAGGACT TTCTGCACAA GGACCGGGAT GACGTCAATG 
     2341 TGGAGATCGT TTTTCTTCAC AACATCTCCC CCAACCTGGA GCTTGAAGCT CTGTTCAAAC 
     2401 GACATTTTAC TCAGGTGGAA TTTTATCAGG GTTCCGTCCT CAATCCACAT GATCTTGCAA 
     2461 GAGTCAAGAT AGAGTCAGCA GATGCATGCC TGATCCTTGC CAACAAGTAC TGCGCTGACC 
     2521 CGGATGCGGA GGATGCCTCG AATATCATGA GAGTAATCTC CATAAAGAAC TACCATCCGA 
     2581 AGATAAGAAT CATCACTCAA ATGCTGCAGT ATCACAACAA GGCCCATCTG CTAAACATCC 
     2641 CGAGCTGGAA TTGGAAAGAA GGTGATGACG CAATCTGCCT CGCAGAGTTG AAGTTGGGCT 
     2701 TCATAGCCCA GAGCTGCCTG GCTCAAGGCC TCTCCACCAT GCTTGCCAAC CTCTTCTCCA 
     2761 TGAGGTCATT CATAAAGATT GAGGAAGACA CATGGCAGAA ATACTACTTG GAAGGAGTCT 
     2821 CAAATGAAAT GTACACAGAA TATCTCTCCA GTGCCTTCGT GGGTCTGTCC TTCCCTACTG 
     2881 TTTGTGAGCT GTGTTTTGTG AAGCTCAAGC TCCTAATGAT AGCCATTGAG TACAAGTCTG 
     2941 CCAACCGAGA GAGCCGTATA TTAATTAATC CTGGAAACCA TCTTAAGATC CAAGAAGGTA 
     3001 CTTTAGGATT TTTCATCGCA AGTGATGCCA AAGAAGTTAA AAGGGCATTT TTTTACTGCA 
     3061 AGGCCTGTCA TGATGACATC ACAGATCCCA AAAGAATAAA AAAATGTGGC TGCAAACGGC 
     3121 TTGAAGATGA GCAGCCGTCA ACACTATCAC CAAAAAAAAA GCAACGGAAT GGAGGCATGC 
     3181 GGAACTCACC CAACACCTCG CCTAAGCTGA TGAGGCATGA CCCCTTGTTA ATTCCTGGCA 
     3241 ATGATCAGAT TGACAACATG GACTCCAATG TGAAGAAGTA CGACTCTACT GGGATGTTTC 
     3301 ACTGGTGTGC ACCCAAGGAG ATAGAGAAAG TCATCCTGGT CACAGGCTGG ATGCCGTATC 
     3361 TGGGACCTAG GGTTTTAATG ACTTGCCTGG ATATTGGTGT AGTATGCATG CCAACTGATA 
     3421 TTCAATCAAC ATCTCCTGCC AGCATAAAAA AGTTTAAGGA GACTCGAAGT GAAGCTGCCA 
     3481 TGACCGTCCT GAGTGGCCAT GTCGTGGTCT GCATCTTTGG CGACGTCAGC TCAGCCCTGA 
     3541 TCGGCCTCCG GAACCTGGTG ATGCCGCTCC GTGCCAGCAA CTTTCATTAC CATGAGCTCA 
     3601 AGCACATTGT GTTTGTGGGC TCTATTGAGT ACCTCAAGCG GGAATGGGAG ACGCTTCATA 
     3661 ACTTCCCCAA AGTGTCCATA TTGCCTGGTA CGCCATTAAG TCGGGCTGAT TTAAGGGCTG 
     3721 TCAACATCAA CCTCTGTGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT AATATTGATG 
     3781 ATACTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA TCTATGCAGT 
     3841 TTGATGACAG CATCGGAGTC TTGCAGGCTA ATTCCCAAGG GTTCACACCT CCAGGAATGG 
     3901 ATAGATCCTC TCCAGATAAC AGCCCAGTGC ACGGGATGTT ACGTCAACCA TCCATCACAA 
     3961 CTGGGGTCAA CATCCCCATC ATCACTGAAC TAGTGAACGA TACTAATGTT CAGTTTTTGG 
     4021 ACCAAGACGA TGATGATGAC CCTGATACAG AACTGTACCT CACGCAGCCC TTTGCCTGTG 
     4081 GGACAGCATT TGCCGTCAGT GTCCTGGACT CACTCATGAG CGCGACGTAC TTCAATGACA 
     4141 ATATCCTCAC CCTGATACGG ACCCTGGTGA CCGGAGGAGC CACGCCGGAG CTGGAGGCTC 
     4201 TGATTGCTGA GGAAAACGCC CTTAGAGGTG GCTACAGCAC CCCGCAGACA CTGGCCAATA 
     4261 GGGACCGCTG CCGCGTGGCC CAGTTAGCTC TGCTCGATGG GCCATTTGCG GACTTAGGGG 
     4321 ATGGTGGTTG TTATGGTGAT CTGTTCTGCA AAGCTCTGAA AACATATAAT ATGCTTTGTT 
     4381 TTGGAATTTA CCGGCTGAGA GATGCTCACC TCAGCACCCC CAGTCAGTGC ACAAAGAGGT 
     4441 ATGTCATCAC CAACCCGCCC TATGAGTTTG AGCTCGTGCC GACGGACCTG ATCTTCTGCT 
     4501 TAATGCAGTT TGACCACAAT GCCGGCCAGT CCCGGGCCAG CCTGTCCCAT TCCTCCCACT 
     4561 CGTCGCAGTC CTCCAGCAAG AAGAGCTCCT CTGTTCACTC CATCCCATCC ACAGCAAACC 
     4621 GACAGAACCG GCCCAAGTCC AGGGAGTCCC GGGACAAACA GAAGTACGTG CAGGAAGAGC 
     4681 GGCTTAGCGG ACCGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4741 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4801 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4861 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4921 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4981 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5041 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5101 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5161 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5221 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5281 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5341 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5401 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5461 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5521 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5581 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5641 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5701 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5761 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5821 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5881 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5941 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6001 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6061 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6121 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6181 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6241 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6301 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6361 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6421 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6481 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6541 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6601 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6661 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6721 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6781 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6841 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6901 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6961 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7021 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7081 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7141 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7201 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7261 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7321 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7381 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7441 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7501 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7561 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7621 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7681 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7741 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7801 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7861 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7921 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7981 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8041 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8101 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8161 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8221 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8281 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8341 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8401 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8461 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8521 ATTTAACAAA AATTTAACGC GAATTTT
//