LOCUS       RC228649                8412 bp    DNA     circular  SYN 
DEFINITION  RPTOR (Myc-DDK-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 2
ACCESSION   NM_001163034
VERSION     NM_001163034.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4569..4660 
                     /label="Myc-DDK"  
     misc_feature    4690..5275 
                     /label="PolyA signal"  
     misc_feature    4747..4766 
                     /label="Restriction Enzyme"  
     misc_feature    5551..6223 
                     /label="ColE1"  
     misc_feature    6653..7447 
                     /label="Kan/Neo"  
     misc_feature    7482..7784 
                     /label="SV40 ori"  
     misc_feature    7870..8347 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4551..4557 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4551 
                     /label="RPTOR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCCGAA ATGCTGCAAT CGCCTCTTCT GGGCCTGGGG GAGGAAGATG 
     1081 AGGCTGATCT TACAGACTGG AACCTACCTT TGGCTTTTAT GAAAAAGAGG CACTGTGAGA 
     1141 AAATTGAAGG CTCCAAATCC TTAGCTCAGA GCTGGAGGAT GAAGGATCGG ATGAAGACAG 
     1201 TCAGTGTTGC CTTAGTTTTG TGCCTGAATG TTGGTGTGGA CCCTCCCGAT GTGGTGAAGA 
     1261 CCACGCCCTG TGCACGCTTG GAATGCTGGA TCGATCCTCT GTCGATGGGT CCTCAGAAAG 
     1321 CTCTGGAAAC CATCGGTGCA AATTTACAGA AGCAGTACGA GAACTGGCAG CCAAGGGCCC 
     1381 GGTACAAGCA GAGCCTTGAC CCAACTGTGG ATGAAGTCAA GAAGCTCTGC ACGTCCTTAC 
     1441 GTCGCAACGC CAAGGAGGAG CGAGTCCTCT TTCACTACAA TGGCCACGGG GTGCCCCGGC 
     1501 CCACAGTCAA CGGGGAGGTC TGGGTCTTCA ACAAGAACTA CACGCAGTAC ATCCCTCTGT 
     1561 CCATATATGA CCTGCAGACG TGGATGGGCA GCCCGTCGAT CTTCGTCTAC GACTGCTCCA 
     1621 ATGCTGGCTT GATCGTCAAG TCCTTCAAGC AGTTCGCACT ACAGCGGGAG CAGGAGCTGG 
     1681 AGGTAGCTGC AATCAACCCA AATCACCCTC TTGCTCAGAT GCCTTTGCCT CCGTCGATGA 
     1741 AAAACTGCAT CCAGCTGGCA GCCTGCGAGG CCACCGAGCT GCTGCCCATG ATCCCCGACC 
     1801 TCCCGGCTGA CCTATTCACC TCCTGCCTCA CCACCCCCAT CAAGATCGCC CTGCGCTGGT 
     1861 TTTGCATGCA GAAATGTGTC AGTCTGGTGC CTGGCGTCAC ACTGGATTTG ATAGAAAAGA 
     1921 TCCCTGGCCG CCTGAACGAC AGGAGGACGC CCCTGGGTGA ACTGAACTGG ATCTTCACAG 
     1981 CCATCACAGA CACCATCGCG TGGAACGTGC TCCCCCGGGA TCTCTTCCAA AAGCTCTTCA 
     2041 GACAGGACTT GCTGGTGGCT AGTCTGTTTC GAAATTTTTT ATTGGCGGAA AGGATTATGA 
     2101 GGTCGTATAA CTGCACTCCC GTCAGCAGCC CGCGTCTGCC GCCCACGTAC ATGCACGCCA 
     2161 TGTGGCAAGC CTGGGACCTG GCTGTTGACA TCTGTCTGTC TCAGCTGCCG ACGATCATCG 
     2221 AGGAAGGCAC TGCGTTTCGG CACAGCCCGT TCTTCGCCGA GCAGCTGACC GCATTCCAGG 
     2281 TGTGGCTCAC CATGGGCGTG GAGAACCGAA ACCCACCCGA ACAGCTGCCC ATCGTCCTGC 
     2341 AGGTGCTGTT AAGCCAAGTG CACCGGCTGA GAGCATTGGA CTTGCTTGGA AGATTTTTGG 
     2401 ACCTGGGTCC CTGGGCAGTG AGCCTGGCCT TGTCTGTCGG CATCTTCCCC TACGTGCTGA 
     2461 AGCTGCTCCA GAGCTCGGCC CGAGAGCTGC GGCCACTTCT CGTTTTCATC TGGGCCAAGA 
     2521 TCCTCGCAGT GGACAGCGAG CTGGTGGTGG CTCTGAGTCA TCTTGTGGTT CAGTATGAAA 
     2581 GCAATTTCTG CACCGTGGCC CTGCAGTTCA TAGAAGAGGA AAAGAACTAC GCCTTGCCTT 
     2641 CTCCAGCAAC CACAGAGGGA GGGAGTTTGA CCCCAGTGCG AGACAGCCCG TGCACCCCCA 
     2701 GACTTCGTTC TGTGAGCTCC TATGGAAACA TCCGTGCTGT CGCCACAGCC AGGAGCCTCA 
     2761 ACAAATCTTT GCAGAACCTG AGTTTGACAG AGGAATCTGG TGGCGCGGTG GCGTTCTCCC 
     2821 CCGGAAACCT CAGCACCAGC AGCAGCGCCA GCAGCACCCT GGGCAGCCCC GAGAATGAGG 
     2881 AGCATATCCT GTCCTTCGAG ACCATCGACA AGATGCGCCG CGCCAGCTCC TACTCCTCCC 
     2941 TCAACTCCCT CATCGGAGTT TCCTTTAACA GTGTTTACAC TCAGATTTGG AGAGTCCTGC 
     3001 TGCACCTGGC TGCTGACCCC TATCCAGAGG TCTCGGACGT GGCCATGAAA GTACTCAACA 
     3061 GCATCGCCTA CAAGGCCACC GTGAACGCCC GGCCGCAGCG CGTCCTGGAC ACCTCCTCCC 
     3121 TCACGCAGTC GGCCCCCGCC AGCCCCACCA ACAAGGGCGT GCACATCCAC CAGGCGGGGG 
     3181 GCTCCCCTCC GGCGTCCAGC ACCAGCAGCT CCAGCCTGAC CAACGATGTG GCCAAGCAGC 
     3241 CGGTCAGCCG AGACTTGCCT TCTGGCCGGC CGGGCACCAC AGGCCCCGCT GGGGCGCAGT 
     3301 ACACCCCTCA CTCCCACCAG TTCCCCCGGA CACGGAAGAT GTTCGACAAG GGCCCAGAGC 
     3361 AGACTGCGGA CGACGCGGAC GATGCTGCTG GACACAAAAG TTTCATCTCC GCCACGGTGC 
     3421 AGACGGGGTT CTGCGACTGG AGCGCCCGCT ATTTTGCCCA GCCCGTCATG AAGATCCCAG 
     3481 AAGAGCACGA CCTGGAGAGT CAGATCCGCA AGGAGCGGGA GTGGCGGTTC CTGCGAAACA 
     3541 GCCGTGTCAG GAGGCAGGCC CAGCAAGTCA TTCAGAAGGG CATTACGAGA TTGGACGACC 
     3601 AAATATTTCT GAACAGGAAC CCCGGCGTCC CCTCTGTGGT GAAATTCCAC CCCTTCACGC 
     3661 CGTGCATCGC CGTAGCCGAC AAGGACAGCA TCTGCTTTTG GGACTGGGAG AAAGGGGAGA 
     3721 AGCTGGATTA TTTCCACAAT GGGAACCCTC GGTACACGAG GGTCACTGCC ATGGAGTATC 
     3781 TGAACGGCCA GGACTGCTCG CTTCTGCTGA CGGCCACAGA CGATGGTGCC ATCAGGGTCT 
     3841 GGAAGAATTT TGCTGATTTG GAAAAGAACC CAGAGATGGT GACCGCGTGG CAGGGGCTCT 
     3901 CGGACATGCT GCCAACGACG CGAGGAGCTG GGATGGTGGT GGACTGGGAG CAGGAGACCG 
     3961 GCCTCCTCAT GAGCTCAGGA GACGTGCGGA TCGTCCGGAT CTGGGACACA GACCGTGAGA 
     4021 TGAAGGTGCA GGACATCCCT ACGGGCGCAG ACAGCTGTGT GACGAGTCTG TCCTGTGATT 
     4081 CCCACCGCTC ACTCATCGTG GCTGGCCTCG GTGACGGCTC CATCCGCGTC TACGACAGAA 
     4141 GGATGGCACT CAGCGAATGC CGCGTCATGA CGTACCGGGA GCACACAGCC TGGGTGGTGA 
     4201 AGGCCTCCCT GCAGAAGCGT CCCGACGGCC ACATCGTGAG TGTGAGCGTC AATGGAGATG 
     4261 TGCGCATCTT TGATCCCCGG ATGCCTGAGT CGGTAAATGT GCTTCAGATC GTGAAGGGGC 
     4321 TGACGGCCCT GGACATCCAC CCCCAGGCGG ACCTGATCGC ATGTGGCTCC GTCAATCAGT 
     4381 TCACCGCCAT CTACAACAGC AGCGGAGAGC TCATCAACAA CATCAAGTAC TACGACGGCT 
     4441 TCATGGGCCA GCGGGTCGGC GCCATCAGCT GCCTGGCCTT CCACCCGCAC TGGCCTCACC 
     4501 TGGCCGTGGG AAGCAACGAC TACTACATCT CCGTGTACTC GGTGGAGAAG CGTGTCAGAA 
     4561 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4621 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4681 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4741 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4801 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4861 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4921 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4981 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5041 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5101 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5161 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5221 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5281 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5341 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5401 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5461 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5521 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5581 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5641 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5701 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5761 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5821 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5881 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5941 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6001 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6061 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6121 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6181 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6241 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6301 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6361 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6421 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6481 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6541 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6601 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6661 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6721 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6781 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6841 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6901 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6961 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7021 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7081 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7141 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7201 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7261 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7321 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7381 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7441 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7501 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7561 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7621 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7741 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7801 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7861 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7921 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7981 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8041 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8101 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8161 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8221 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8281 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8341 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8401 AACGCGAATT TT
//