LOCUS       RC228646                8370 bp    DNA     circular  SYN 
DEFINITION  TSC1 (Myc-DDK-tagged)-Human tuberous sclerosis 1 (TSC1), transcript variant 3
ACCESSION   NM_001162426
VERSION     NM_001162426.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4527..4618 
                     /label="Myc-DDK"  
     misc_feature    4648..5233 
                     /label="PolyA signal"  
     misc_feature    4705..4724 
                     /label="Restriction Enzyme"  
     misc_feature    5509..6181 
                     /label="ColE1"  
     misc_feature    6611..7405 
                     /label="Kan/Neo"  
     misc_feature    7440..7742 
                     /label="SV40 ori"  
     misc_feature    7828..8305 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4509..4515 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4509 
                     /label="TSC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAACAA GCAAATGTCG GGGAGCTTCT TGCCATGCTG GACTCCCCCA 
     1081 TGCTGGGTGT GCGGGACGAC GTGACAGCTG TCTTTAAAGA GAACCTCAAT TCTGACCGTG 
     1141 GCCCTATGCT TGTAAACACC TTGGTGGATT ATTACCTGGA AACCAGCTCT CAGCCGGCAT 
     1201 TGCACATCCT GACCACCTTG CAAGAGCCAC ATGACAAGCA CCTCTTGGAC AGGATTAACG 
     1261 AATATGTGGG CAAAGCCGCC ACTCGTTTAT CCATCCTCTC GTTACTGGGT CATGTCATAA 
     1321 GACTGCAGCC ATCTTGGAAG CATAAGCTCT CTCAAGCACC TCTTTTGCCT TCTTTACTAA 
     1381 AATGTCTCAA GATGGACACT GACGTCGTTG TCCTCACAAC AGGCGTCTTG GTGTTGATAA 
     1441 CCATGCTACC AATGATTCCA CAGTCTGGGA AACAGCATCT TCTTGATTTC TTTGACATTT 
     1501 TTGGCCGTCT GTCATCATGG TGCCTGAAGA AACCAGGCCA CGTGGCGGAA GTCTATCTCG 
     1561 TCCATCTCCA TGCCAGTGTG TACGCACTCT TTCATCGCCT TTATGGAATG TACCCTTGCA 
     1621 ACTTCGTCTC CTTTTTGCGT TCTCATTACA GTATGAAAGA AAACCTGGAG ACTTTTGAAG 
     1681 AAGTGGTCAA GCCAATGATG GAGCATGTGC GAATTCATCC GGAATTAGTG ACTGGATCCA 
     1741 AGGACCATGA ACTGGACCCT CGAAGGTGGA AGAGATTAGA AACTCATGAT GTTGTGATCG 
     1801 AGTGTGCCAA AATCTCTCTG GATCCCACAG AAGCCTCATA TGAAGATGGC TATTCTGTGT 
     1861 CTCACCAAAT CTCAGCCCGC TTTCCTCATC GTTCAGCCGA TGTCACCACC AGCCCTTATG 
     1921 CTGACACACA GAATAGCTAT GGGTGTGCTA CTTCTACCCC TTACTCCACG TCTCGGCTGA 
     1981 TGTTGTTAAA TATGCCAGGG CAGCTACCTC AGACTCTGAG TTCCCCATCG ACACGGCTGA 
     2041 TAACTGAACC ACCACAAGCT ACTCTTTGGA GCCCATCTAT GGTTTGTGGT ATGACCACTC 
     2101 CTCCAACTTC TCCTGGAAAT GTCCCACCTG ATCTGTCACA CCCTTACAGT AAAGTCTTTG 
     2161 GTACAACTGG TGGAAAAGGA ACTCCTCTGG GAACCCCAGC AACCTCTCCT CCTCCAGCCC 
     2221 CACTCTGTCA TTCGGATGAC TACGTGCACA TTTCACTCCC CCAGGCCACA GTCACACCCC 
     2281 CCAGGAAGGA AGAGAGAATG GATTCTGCAA GACCATGTCT ACACAGACAA CACCATCTTC 
     2341 TGAATGACAG AGGATCAGAA GAGCCACCTG GCAGCAAAGG TTCTGTCACT CTAAGTGATC 
     2401 TTCCAGGGTT TTTAGGTGAT CTGGCCTCTG AAGAAGATAG TATTGAAAAA GATAAAGAAG 
     2461 AAGCTGCAAT ATCTAGAGAA CTTTCTGAGA TCACCACAGC AGAGGCAGAG CCTGTGGTTC 
     2521 CTCGAGGAGG CTTTGACTCT CCCTTTTACC GAGACAGTCT CCCAGGTTCT CAGCGGAAGA 
     2581 CCCACTCGGC AGCCTCCAGT TCTCAGGGCG CCAGCGTGAA CCCTGAGCCT TTACACTCCT 
     2641 CCCTGGACAA GCTTGGGCCT GACACACCAA AGCAAGCCTT TACTCCCATA GACCTGCCCT 
     2701 GCGGCAGTGC TGATGAAAGC CCTGCGGGAG ACAGGGAATG CCAGACTTCT TTGGAGACCA 
     2761 GTATCTTCAC TCCCAGTCCT TGTAAAATTC CACCTCCGAC GAGAGTGGGC TTTGGAAGCG 
     2821 GGCAGCCTCC CCCGTATGAT CATCTTTTTG AGGTGGCATT GCCAAAGACA GCCCATCATT 
     2881 TTGTCATCAG GAAGACTGAG GAGCTGTTAA AGAAAGCAAA AGGAAACACA GAGGAAGATG 
     2941 GTGTGCCCTC TACCTCCCCA ATGGAAGTGC TGGACAGACT GATACAGCAG GGAGCAGACG 
     3001 CGCACAGCAA GGAGCTGAAC AAGTTGCCTT TACCCAGCAA GTCTGTCGAC TGGACCCACT 
     3061 TTGGAGGCTC TCCTCCTTCA GATGAGATCC GCACCCTCCG AGACCAGTTG CTTTTACTGC 
     3121 ACAACCAGTT ACTCTATGAG CGTTTTAAGA GGCAGCAGCA TGCCCTCCGG AACAGGCGGC 
     3181 TCCTCCGCAA GGTGATCAAA GCAGCAGCTC TGGAGGAACA TAATGCTGCC ATGAAAGATC 
     3241 AGTTGAAGTT ACAAGAGAAG GACATCCAGA TGTGGAAGGT TAGTCTGCAG AAAGAACAAG 
     3301 CTAGATACAA TCAGCTCCAG GAGCAGCGTG ACACTATGGT AACCAAGCTC CACAGCCAGA 
     3361 TCAGACAGCT GCAGCATGAC CGAGAGGAAT TCTACAACCA GAGCCAGGAA TTACAGACGA 
     3421 AGCTGGAGGA CTGCAGGAAC ATGATTGCGG AGCTGCGGAT AGAACTGAAG AAGGCCAACA 
     3481 ACAAGGTGTG TCACACTGAG CTGCTGCTCA GTCAGGTTTC CCAAAAGCTC TCAAACAGTG 
     3541 AGTCGGTCCA GCAGCAGATG GAGTTCTTGA ACAGGCAGCT GTTGGTTCTT GGGGAGGTCA 
     3601 ACGAGCTCTA TTTGGAACAA CTGCAGAACA AGCACTCAGA TACCACAAAG GAAGTAGAAA 
     3661 TGATGAAAGC CGCCTATCGG AAAGAGCTAG AAAAAAACAG AAGCCATGTT CTCCAGCAGA 
     3721 CTCAGAGGCT TGATACCTCC CAAAAACGGA TTTTGGAACT GGAATCTCAC CTGGCCAAGA 
     3781 AAGACCACCT TCTTTTGGAA CAGAAGAAAT ATCTAGAGGA TGTCAAACTC CAGGCAAGAG 
     3841 GACAGCTGCA GGCCGCAGAG AGCAGGTATG AGGCTCAGAA AAGGATAACC CAGGTGTTTG 
     3901 AATTGGAGAT CTTAGATTTA TATGGCAGGT TGGAGAAAGA TGGCCTCCTG AAAAAACTTG 
     3961 AAGAAGAAAA AGCAGAAGCA GCTGAAGCAG CAGAAGAAAG GCTTGACTGT TGTAATGACG 
     4021 GGTGCTCAGA TTCCATGGTA GGGCACAATG AAGAGGCATC TGGCCACAAC GGTGAGACCA 
     4081 AGACCCCCAG GCCCAGCAGC GCCCGGGGCA GTAGTGGAAG CAGAGGTGGT GGAGGCAGCA 
     4141 GCAGCAGCAG CAGCGAGCTT TCTACCCCAG AGAAACCCCC ACACCAGAGG GCAGGCCCAT 
     4201 TCAGCAGTCG GTGGGAGACG ACTATGGGAG AAGCGTCTGC CAGCATCCCC ACCACTGTGG 
     4261 GCTCACTTCC CAGTTCAAAA AGCTTCCTGG GTATGAAGGC TCGAGAGTTA TTTCGTAATA 
     4321 AGAGCGAGAG CCAGTGTGAT GAGGACGGCA TGACCAGTAG CCTTTCTGAG AGCCTAAAGA 
     4381 CAGAACTGGG CAAAGACTTG GGTGTGGAAG CCAAGATTCC CCTGAACCTA GATGGCCCTC 
     4441 ACCCGTCTCC CCCGACCCCG GACAGTGTTG GACAGCTACA TATCATGGAC TACAATGAGA 
     4501 CTCATCATGA ACACAGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4561 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4621 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4681 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4741 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4801 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4861 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4921 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4981 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5041 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5101 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5161 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5221 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5281 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5341 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5401 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5461 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5521 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5581 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5641 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5701 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5761 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5821 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5881 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5941 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6001 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6061 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6121 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6181 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6241 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6301 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6361 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6421 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6481 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6541 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6601 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6661 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6721 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6781 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6841 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6901 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6961 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7021 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7081 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7141 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7201 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7261 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7321 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7381 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7441 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7501 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7561 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7621 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7681 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7741 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7801 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7861 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7921 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7981 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8041 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8101 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8161 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8221 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8281 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8341 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//