LOCUS       RC228642                8238 bp    DNA     circular  SYN 
DEFINITION  WWC1 (Myc-DDK-tagged)-Human WW and C2 domain containing 1 (WWC1), transcript variant 1
ACCESSION   NM_001161661
VERSION     NM_001161661.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4395..4486 
                     /label="Myc-DDK"  
     misc_feature    4516..5101 
                     /label="PolyA signal"  
     misc_feature    4573..4592 
                     /label="Restriction Enzyme"  
     misc_feature    5377..6049 
                     /label="ColE1"  
     misc_feature    6479..7273 
                     /label="Kan/Neo"  
     misc_feature    7308..7610 
                     /label="SV40 ori"  
     misc_feature    7696..8173 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4377..4383 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4377 
                     /label="WWC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGGCCG GAGCTGCCCC TGCCGGAGGG CTGGGAGGAG GCGCGCGACT 
     1081 TCGACGGCAA GGTCTACTAC ATAGACCACA CGAACCGCAC CACCAGCTGG ATCGACCCGC 
     1141 GGGACAGGTA CACCAAACCG CTCACCTTTG CTGACTGCAT TAGTGATGAG TTGCCGCTAG 
     1201 GATGGGAAGA GGCATATGAC CCACAGGTTG GAGATTACTT CATAGACCAC AACACCAAAA 
     1261 CCACTCAGAT TGAGGATCCT CGAGTACAAT GGCGGCGGGA GCAGGAACAT ATGCTGAAGG 
     1321 ATTACCTGGT GGTGGCCCAG GAGGCTCTGA GTGCACAAAA GGAGATCTAC CAGGTGAAGC 
     1381 AGCAGCGCCT GGAGCTTGCA CAGCAGGAGT ACCAGCAACT GCATGCCGTC TGGGAGCATA 
     1441 AGCTGGGCTC CCAGGTCAGC TTGGTCTCTG GTTCATCATC CAGCTCCAAG TATGACCCTG 
     1501 AGATCCTGAA AGCTGAAATT GCCACTGCAA AATCCCGGGT CAACAAGCTG AAGAGAGAGA 
     1561 TGGTTCACCT CCAGCACGAG CTGCAGTTCA AAGAGCGTGG CTTTCAGACC CTGAAGAAAA 
     1621 TCGATAAGAA AATGTCTGAT GCTCAGGGCA GCTACAAACT GGATGAAGCT CAGGCTGTCT 
     1681 TGAGAGAAAC AAAAGCCATC AAAAAGGCTA TTACCTGTGG GGAAAAGGAA AAGCAAGATC 
     1741 TCATTAAGAG CCTTGCCATG TTGAAGGACG GCTTCCGCAC TGACAGGGGG TCTCACTCAG 
     1801 ACCTGTGGTC CAGCAGCAGC TCTCTGGAGA GTTCGAGTTT CCCGCTACCG AAACAGTACC 
     1861 TGGATGTGAG CTCCCAGACA GACATCTCGG GAAGCTTCGG CATCAACAGC AACAATCAGT 
     1921 TGGCAGAGAA GGTCAGATTG CGCCTTCGAT ATGAAGAGGC TAAGAGAAGG ATCGCCAACC 
     1981 TGAAGATCCA GCTGGCCAAG CTTGACAGTG AGGCCTGGCC TGGGGTGCTG GACTCAGAGA 
     2041 GGGACCGGCT GATCCTTATC AACGAGAAGG AGGAGCTGCT GAAGGAGATG CGCTTCATCA 
     2101 GCCCCCGCAA GTGGACCCAG GGGGAGGTGG AGCAGCTGGA GATGGCCCGG AAGCGGCTGG 
     2161 AAAAGGACCT GCAGGCAGCC CGGGACACCC AGAGCAAGGC GCTGACGGAG AGGTTAAAGT 
     2221 TAAACAGTAA GAGGAACCAG CTTGTGAGAG AACTGGAGGA AGCCACCCGG CAGGTGGCAA 
     2281 CTCTGCACTC CCAGCTGAAA AGTCTCTCAA GCAGCATGCA GTCCCTGTCC TCAGGCAGCA 
     2341 GCCCCGGATC CCTCACGTCC AGCCGGGGCT CCCTGGTTGC ATCCAGCCTG GACTCCTCCA 
     2401 CTTCAGCCAG CTTCACTGAC CTCTACTATG ACCCCTTTGA GCAGCTGGAC TCAGAGCTGC 
     2461 AGAGCAAGGT GGAGTTCCTG CTCCTGGAGG GGGCCACCGG CTTCCGGCCC TCAGGCTGCA 
     2521 TCACCACCAT CCACGAGGAT GAGGTGGCCA AGACCCAGAA GGCAGAGGGA GGTGGCCGCC 
     2581 TGCAGGCTCT GCGTTCCCTG TCTGGCACCC CAAAGTCCAT GACCTCCCTA TCCCCACGTT 
     2641 CCTCTCTCTC CTCCCCCTCC CCACCCTGTT CCCCTCTCAT GGCTGACCCC CTCCTGGCTG 
     2701 GTGATGCCTT CCTCAACTCC TTGGAGTTTG AAGACCCGGA GCTGAGTGCC ACTCTTTGTG 
     2761 AACTGAGCCT TGGTAACAGC GCCCAGGAAA GATACCGGCT GGAGGAACCA GGAACGGAGG 
     2821 GCAAGCAGCT GGGCCAAGCT GTGAATACGG CCCAGGGGTG TGGCCTGAAA GTGGCCTGTG 
     2881 TCTCAGCCGC CGTATCGGAC GAGTCAGTGG CTGGAGACAG TGGTGTGTAC GAGGCTTCCG 
     2941 TGCAGAGACT GGGTGCTTCA GAAGCTGCTG CATTTGACAG TGACGAATCG GAAGCAGTGG 
     3001 GTGCGACCCG AATTCAGATT GCCCTGAAGT ATGATGAGAA GAATAAGCAA TTTGCAATAT 
     3061 TAATCATCCA GCTGAGTAAC CTTTCTGCTC TGTTGCAGCA ACAAGACCAG AAAGTGAATA 
     3121 TCCGCGTGGC TGTCCTTCCT TGCTCTGAAA GCACAACCTG CCTGTTCCGG ACCCGGCCTC 
     3181 TGGACGCCTC AGACACTCTA GTGTTCAATG AGGTGTTCTG GGTATCCATG TCCTATCCAG 
     3241 CCCTTCACCA GAAGACCTTA AGAGTCGATG TCTGTACCAC CGACAGGAGC CATCTGGAAG 
     3301 AGTGCCTGGG AGGCGCCCAG ATCAGCCTGG CGGAGGTCTG CCGGTCTGGG GAGAGGTCGA 
     3361 CTCGCTGGTA CAACCTTCTC AGCTACAAAT ACTTGAAGAA ACAGAGCAGG GAGCTCAAGC 
     3421 CAGTGGGAGT CATGGCCCCT GCCTCAGGGC CTGCCAGCAC GGACGCTGTG TCTGCTCTGT 
     3481 TGGAACAGAC AGCAGTGGAG CTGGAGAAGA GGCAGGAGGG CAGGAGCAGC ACACAGACAC 
     3541 TGGAAGACAG CTGGAGGTAT GAGGAGACCA GTGAGAATGA GGCAGTAGCC GAGGAAGAGG 
     3601 AGGAGGAGGT GGAGGAGGAG GAGGGAGAAG AGGATGTTTT CACCGAGAAA GCCTCACCTG 
     3661 ATATGGATGG GTACCCAGCA TTAAAGGTGG ACAAAGAGAC CAACACGGAG ACCCCGGCCC 
     3721 CATCCCCCAC AGTGGTGCGA CCTAAGGACC GGAGAGTGGG CACCCCGTCC CAGGGGCCAT 
     3781 TTCTTCGAGG GAGCACCATC ATCCGCTCTA AGACCTTCTC CCCAGGACCC CAGAGCCAGT 
     3841 ACGTGTGCCG GCTGAATCGG AGTGATAGTG ACAGCTCCAC TCTGTCCAAA AAGCCACCTT 
     3901 TTGTTCGAAA CTCCCTGGAG CGACGCAGCG TCCGGATGAA GCGGCCGTCC CCACCCCCAC 
     3961 AGCCTTCCTC GGTCAAGTCG CTGCGCTCCG AGCGTCTGAT CCGTACCTCG CTGGACCTGG 
     4021 AGTTAGACCT GCAGGCGACA AGAACCTGGC ACAGCCAATT GACCCAGGAG ATCTCGGTGC 
     4081 TGAAGGAGCT CAAGGAGCAG CTGGAACAAG CCAAGAGCCA CGGGGAGAAG GAGCTGCCAC 
     4141 AGTGGTTGCG TGAGGACGAG CGTTTCCGCC TGCTGCTGAG GATGCTGGAG AAGCGGCAGA 
     4201 TGGACCGAGC GGAGCACAAG GGTGAGCTTC AGACAGACAA GATGATGAGG GCAGCTGCCA 
     4261 AGGATGTGCA CAGGCTCCGA GGCCAGAGCT GTAAGGAACC CCCAGAAGTT CAGTCTTTCA 
     4321 GGGAGAAGAT GGCATTTTTC ACCCGGCCTC GGATGAATAT CCCAGCTCTC TCTGCAGATG 
     4381 ACGTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4441 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4501 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4561 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4621 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4681 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4741 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4801 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4861 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4921 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4981 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5041 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5101 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5161 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5221 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5281 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5341 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5401 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5461 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5521 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5581 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5641 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5701 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5761 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5821 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5881 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5941 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6001 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6061 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6121 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6181 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6241 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6301 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6361 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6421 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6481 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6541 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6601 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6661 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6721 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6781 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6841 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6901 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6961 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7021 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7081 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7141 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7201 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7261 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7321 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7381 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7441 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7501 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7561 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7621 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7681 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7741 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7801 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7861 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7921 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7981 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8041 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8101 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8161 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8221 AAATTTAACG CGAATTTT
//