LOCUS       RC228641                8235 bp    DNA     circular  SYN 
DEFINITION  WWC1 (Myc-DDK-tagged)-Human WW and C2 domain containing 1 (WWC1), transcript variant 2
ACCESSION   NM_001161662
VERSION     NM_001161662.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4392..4483 
                     /label="Myc-DDK"  
     misc_feature    4513..5098 
                     /label="PolyA signal"  
     misc_feature    4570..4589 
                     /label="Restriction Enzyme"  
     misc_feature    5374..6046 
                     /label="ColE1"  
     misc_feature    6476..7270 
                     /label="Kan/Neo"  
     misc_feature    7305..7607 
                     /label="SV40 ori"  
     misc_feature    7693..8170 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4374..4380 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4374 
                     /label="WWC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGGCCG GAGCTGCCCC TGCCGGAGGG CTGGGAGGAG GCGCGCGACT 
     1081 TCGACGGCAA GGTCTACTAC ATAGACCACA CGAACCGCAC CACCAGCTGG ATCGACCCGC 
     1141 GGGACAGGTA CACCAAACCG CTCACCTTTG CTGACTGCAT TAGTGATGAG TTGCCGCTAG 
     1201 GATGGGAAGA GGCATATGAC CCACAGGTTG GAGATTACTT CATAGACCAC AACACCAAAA 
     1261 CCACTCAGAT TGAGGATCCT CGAGTACAAT GGCGGCGGGA GCAGGAACAT ATGCTGAAGG 
     1321 ATTACCTGGT GGTGGCCCAG GAGGCTCTGA GTGCACAAAA GGAGATCTAC CAGGTGAAGC 
     1381 AGCAGCGCCT GGAGCTTGCA CAGCAGGAGT ACCAGCAACT GCATGCCGTC TGGGAGCATA 
     1441 AGCTGGGCTC CCAGGTCAGC TTGGTCTCTG GTTCATCATC CAGCTCCAAG TATGACCCTG 
     1501 AGATCCTGAA AGCTGAAATT GCCACTGCAA AATCCCGGGT CAACAAGCTG AAGAGAGAGA 
     1561 TGGTTCACCT CCAGCACGAG CTGCAGTTCA AAGAGCGTGG CTTTCAGACC CTGAAGAAAA 
     1621 TCGATAAGAA AATGTCTGAT GCTCAGGGCA GCTACAAACT GGATGAAGCT CAGGCTGTCT 
     1681 TGAGAGAAAC AAAAGCCATC AAAAAGGCTA TTACCTGTGG GGAAAAGGAA AAGCAAGATC 
     1741 TCATTAAGAG CCTTGCCATG TTGAAGGACG GCTTCCGCAC TGACAGGGGG TCTCACTCAG 
     1801 ACCTGTGGTC CAGCAGCAGC TCTCTGGAGA GTTCGAGTTT CCCGCTACCG AAACAGTACC 
     1861 TGGATGTGAG CTCCCAGACA GACATCTCGG GAAGCTTCGG CATCAACAGC AACAATCAGT 
     1921 TGGCAGAGAA GGTCAGATTG CGCCTTCGAT ATGAAGAGGC TAAGAGAAGG ATCGCCAACC 
     1981 TGAAGATCCA GCTGGCCAAG CTTGACAGTG AGGCCTGGCC TGGGGTGCTG GACTCAGAGA 
     2041 GGGACCGGCT GATCCTTATC AACGAGAAGG AGGAGCTGCT GAAGGAGATG CGCTTCATCA 
     2101 GCCCCCGCAA GTGGACCCAG GGGGAGGTGG AGCAGCTGGA GATGGCCCGG AAGCGGCTGG 
     2161 AAAAGGACCT GCAGGCAGCC CGGGACACCC AGAGCAAGGC GCTGACGGAG AGGTTAAAGT 
     2221 TAAACAGTAA GAGGAACCAG CTTGTGAGAG AACTGGAGGA AGCCACCCGG CAGGTGGCAA 
     2281 CTCTGCACTC CCAGCTGAAA AGTCTCTCAA GCAGCATGCA GTCCCTGTCC TCAGGCAGCA 
     2341 GCCCCGGATC CCTCACGTCC AGCCGGGGCT CCCTGGTTGC ATCCAGCCTG GACTCCTCCA 
     2401 CTTCAGCCAG CTTCACTGAC CTCTACTATG ACCCCTTTGA GCAGCTGGAC TCAGAGCTGC 
     2461 AGAGCAAGGT GGAGTTCCTG CTCCTGGAGG GGGCCACCGG CTTCCGGCCC TCAGGCTGCA 
     2521 TCACCACCAT CCACGAGGAT GAGGTGGCCA AGACCCAGAA GGCAGAGGGA GGTGGCCGCC 
     2581 TGCAGGCTCT GCGTTCCCTG TCTGGCACCC CAAAGTCCAT GACCTCCCTA TCCCCACGTT 
     2641 CCTCTCTCTC CTCCCCCTCC CCACCCTGTT CCCCTCTCAT GGCTGACCCC CTCCTGGCTG 
     2701 GTGATGCCTT CCTCAACTCC TTGGAGTTTG AAGACCCGGA GCTGAGTGCC ACTCTTTGTG 
     2761 AACTGAGCCT TGGTAACAGC GCCCAGGAAA GATACCGGCT GGAGGAACCA GGAACGGAGG 
     2821 GCAAGCAGCT GGGCCAAGCT GTGAATACGG CCCAGGGGTG TGGCCTGAAA GTGGCCTGTG 
     2881 TCTCAGCCGC CGTATCGGAC GAGTCAGTGG CTGGAGACAG TGGTGTGTAC GAGGCTTCCG 
     2941 TGCAGAGACT GGGTGCTTCA GAAGCTGCTG CATTTGACAG TGACGAATCG GAAGCAGTGG 
     3001 GTGCGACCCG AATTCAGATT GCCCTGAAGT ATGATGAGAA GAATAAGCAA TTTGCAATAT 
     3061 TAATCATCCA GCTGAGTAAC CTTTCTGCTC TGTTGCAGCA ACAAGACCAG AAAGTGAATA 
     3121 TCCGCGTGGC TGTCCTTCCT TGCTCTGAAA GCACAACCTG CCTGTTCCGG ACCCGGCCTC 
     3181 TGGACGCCTC AGACACTCTA GTGTTCAATG AGGTGTTCTG GGTATCCATG TCCTATCCAG 
     3241 CCCTTCACCA GAAGACCTTA AGAGTCGATG TCTGTACCAC CGACAGGAGC CATCTGGAAG 
     3301 AGTGCCTGGG AGGCGCCCAG ATCAGCCTGG CGGAGGTCTG CCGGTCTGGG GAGAGGTCGA 
     3361 CTCGCTGGTA CAACCTTCTC AGCTACAAAT ACTTGAAGAA ACAGAGCAGG GAGCTCAAGC 
     3421 CAGTGGGAGT CATGGCCCCT GCCTCAGGGC CTGCCAGCAC GGACGCTGTG TCTGCTCTGT 
     3481 TGGAACAGAC AGCAGTGGAG CTGGAGAAGA GGCAGGAGGG CAGGAGCAGC ACACAGACAC 
     3541 TGGAAGACAG CTGGAGGTAT GAGGAGACCA GTGAGAATGA GGCAGTAGCC GAGGAAGAGG 
     3601 AGGAGGAGGT GGAGGAGGAG GAGGGAGAAG AGGATGTTTT CACCGAGAAA GCCTCACCTG 
     3661 ATATGGATGG GTACCCAGCA TTAAAGGTGG ACAAAGAGAC CAACACGGAG ACCCCGGCCC 
     3721 CATCCCCCAC AGTGGTGCGA CCTAAGGACC GGAGAGTGGG CACCCCGTCC CAGGGGCCAT 
     3781 TTCTTCGAGG GAGCACCATC ATCCGCTCTA AGACCTTCTC CCCAGGACCC CAGAGCCAGT 
     3841 ACGTGTGCCG GCTGAATCGG AGTGATAGTG ACAGCTCCAC TCTGTCCAAA AAGCCACCTT 
     3901 TTGTTCGAAA CTCCCTGGAG CGACGCAGCG TCCGGATGAA GCGGCCGTCC CCACCCCCAC 
     3961 AGCCTTCCTC GGTCAAGTCG CTGCGCTCCG AGCGTCTGAT CCGTACCTCG CTGGACCTGG 
     4021 AGTTAGACCT GCAGGCGACA AGAACCTGGC ACAGCCAATT GACCCAGGAG ATCTCGGTGC 
     4081 TGAAGGAGCT CAAGGAGCAG CTGGAACAAG CCAAGAGCCA CGGGGAGAAG GAGCTGCCAC 
     4141 AGTGGTTGCG TGAGGACGAG CGTTTCCGCC TGCTGCTGAG GATGCTGGAG AAGCGGATGG 
     4201 ACCGAGCGGA GCACAAGGGT GAGCTTCAGA CAGACAAGAT GATGAGGGCA GCTGCCAAGG 
     4261 ATGTGCACAG GCTCCGAGGC CAGAGCTGTA AGGAACCCCC AGAAGTTCAG TCTTTCAGGG 
     4321 AGAAGATGGC ATTTTTCACC CGGCCTCGGA TGAATATCCC AGCTCTCTCT GCAGATGACG 
     4381 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4441 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4501 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4561 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4621 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4681 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4741 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4801 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4861 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4921 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4981 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5041 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5101 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5161 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5221 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5281 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5341 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5401 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5461 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5521 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5581 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5641 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5701 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5761 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5821 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5881 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5941 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6001 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6061 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6121 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6181 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6241 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6301 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6361 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6421 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6481 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6541 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6601 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6661 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6721 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6781 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6841 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6901 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6961 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7021 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7081 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7141 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7201 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7261 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7321 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7381 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7441 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7501 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7561 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7621 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7681 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7741 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7801 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7861 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7921 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7981 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8041 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8101 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8161 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8221 TTTAACGCGA ATTTT
//