LOCUS       RC228638                8142 bp    DNA     circular  SYN 
DEFINITION  SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 3
ACCESSION   NM_001145962
VERSION     NM_001145962.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4299..4390 
                     /label="Myc-DDK"  
     misc_feature    4420..5005 
                     /label="PolyA signal"  
     misc_feature    4477..4496 
                     /label="Restriction Enzyme"  
     misc_feature    5281..5953 
                     /label="ColE1"  
     misc_feature    6383..7177 
                     /label="Kan/Neo"  
     misc_feature    7212..7514 
                     /label="SV40 ori"  
     misc_feature    7600..8077 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4281..4287 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4281 
                     /label="SLC12A4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGAGCGGGC GGGGCGTGTC GGCCCGGGGC GGCGGGGACA GCGGCGGGGA 
     1081 CAGCGGCGGG CGGCTGGGAC GGCGGGTGCG GCGGGGCCGA GCCCGCACGA TGCCTCACTT 
     1141 CACCGTGGTG CCAGTGGACG GGCCGAGGCG CGGCGACTAT GACAACCTCG AGGGGCTCAG 
     1201 TTGGGTGGAC TACGGGGAGC GCGCCGAGCT GGATGACTCG GACGGAAGAG CTGGACATCC 
     1261 GCCCAAAGGT ATCGTCTCTT CTGGGAAAGC TCGTCAGCTA CACCAACCTC ACCCAGGGCG 
     1321 CCAAAGAGCA TGAGGAGGCC GAGAGTGGGG AGGGCACCCG CCGGAGGGCA GCCGAGGCAC 
     1381 CCAGCATGGG CACCCTCATG GGGGTGTACC TGCCCTGCCT GCAGAATATC TTTGGGGTTA 
     1441 TCCTCTTCCT GCGGCTGACC TGGATGGTGG GCACAGCAGG TGTGCTACAG GCCCTCCTCA 
     1501 TCGTGCTTAT CTGCTGCTGT TGTACCCTGC TGACGGCCAT CTCCATGAGT GCCATCGCCA 
     1561 CCAACGGTGT GGTTCCAGCT GGGGGCTCCT ATTTCATGAT CTCTCGTTCA CTGGGGCCAG 
     1621 AATTTGGAGG TGCTGTGGGC CTGTGCTTCT ACCTGGGAAC AACATTCGCA GCAGCCATGT 
     1681 ACATCCTGGG GGCCATCGAG ATCTTGCTGA CCTACATTGC CCCACCAGCT GCCATTTTTT 
     1741 ACCCATCGGG TGCTCATGAC ACGTCGAATG CCACTTTGAA CAATATGCGT GTGTATGGGA 
     1801 CCATTTTCCT GACCTTCATG ACCCTGGTGG TGTTTGTGGG GGTCAAGTAT GTGAACAAAT 
     1861 TTGCCTCGCT CTTCCTGGCC TGTGTGATCA TCTCCATCCT CTCCATCTAT GCTGGGGGCA 
     1921 TAAAGTCTAT ATTTGACCCT CCCGTGTTTC CGGTATGCAT GCTGGGCAAC AGGACCCTGT 
     1981 CCCGGGACCA GTTTGACATC TGTGCCAAGA CAGCTGTAGT GGACAATGAG ACAGTGGCCA 
     2041 CCCAGCTATG GAGTTTCTTC TGCCACAGCC CCAACCTTAC GACCGACTCC TGTGACCCCT 
     2101 ACTTCATGCT CAACAATGTG ACCGAGATCC CTGGCATCCC CGGGGCAGCT GCTGGTGTGC 
     2161 TCCAGGAAAA CCTGTGGAGC GCCTACCTGG AGAAGGGTGA CATCGTGGAG AAGCATGGGC 
     2221 TGCCCTCCGC AGATGCCCCG AGCCTGAAGG AGAGCCTGCC TCTGTACGTG GTCGCTGACA 
     2281 TCGCCACATC CTTCACCGTG CTGGTCGGCA TCTTCTTCCC TTCTGTAACA GGCATCATGG 
     2341 CTGGCTCAAA CCGCTCTGGG GACCTTCGTG ACGCCCAGAA GTCTATCCCT GTGGGGACCA 
     2401 TTCTGGCCAT CATTACAACT TCCCTCGTGT ACTTCAGCAG TGTGGTTCTC TTTGGTGCCT 
     2461 GCATTGAGGG TGTGGTTCTC CGGGACAAGT ATGGCGATGG TGTCAGCAGG AACTTGGTGG 
     2521 TGGGCACACT GGCCTGGCCT TCACCCTGGG TCATCGTCAT CGGCTCCTTC TTTTCAACGT 
     2581 GTGGCGCTGG CCTCCAGAGC CTCACAGGGG CACCACGCCT ATTGCAGGCC ATTGCCAAGG 
     2641 ACAACATCAT CCCCTTCCTC CGGGTGTTTG GCCACGGGAA GGTGAATGGT GAACCCACAT 
     2701 GGGCACTCCT CCTGACGGCA CTCATCGCCG AGCTGGGCAT CCTCATCGCC TCCCTCGACA 
     2761 TGGTGGCCCC CATCTTATCC ATGTTCTTTC TGATGTGCTA CCTGTTCGTG AACCTCGCCT 
     2821 GTGCGGTGCA GACACTCCTG AGGACCCCCA ACTGGCGGCC CCGGTTCAAG TACTATCACT 
     2881 GGGCGCTGTC CTTCCTGGGC ATGAGTCTCT GCCTGGCCCT TATGTTTGTC TCCTCCTGGT 
     2941 ACTATGCCCT GGTGGCCATG CTCATCGCCG GCATGATCTA CAAATACATC GAGTACCAAG 
     3001 GGGCTGAGAA GGAGTGGGGT GACGGGATCC GAGGCCTGTC CCTGAGCGCT GCCCGCTACG 
     3061 CGCTGTTGCG GCTGGAGGAG GGGCCTCCTC ACACCAAGAA CTGGCGGCCG CAGCTGCTGG 
     3121 TGCTGCTGAA GCTGGACGAG GACCTCCACG TGAAGTACCC GCGGCTCCTC ACCTTCGCCT 
     3181 CCCAGCTCAA GGCTGGCAAG GGCCTGACCA TTGTTGGTTC TGTCATCCAG GGGAGCTTCT 
     3241 TGGAGAGCTA TGGCGAGGCT CAGGCCGCCG AGCAGACCAT CAAGAACATG ATGGAAATTG 
     3301 AGAAGGTGAA GGGCTTCTGC CAGGTGGTGG TGGCCAGCAA GGTGCGGGAG GGGCTGGCCC 
     3361 ACCTCATCCA GTCCTGTGGC CTGGGAGGCA TGCGGCATAA CTCCGTGGTG CTGGGCTGGC 
     3421 CCTACGGCTG GCGACAGAGC GAGGACCCCC GTGCCTGGAA GACCTTCATT GACACCGTGC 
     3481 GCTGCACTAC GGCTGCCCAC CTGGCCCTGC TCGTGCCCAA GAACATCGCC TTCTACCCCA 
     3541 GCAACCACGA GCGCTACCTG GAGGGCCACA TAGACGTGTG GTGGATCGTG CACGATGGTG 
     3601 GCATGCTCAT GCTTCTGCCC TTCCTGCTGC GCCAGCATAA GGTCTGGAGG AAGTGCCGGA 
     3661 TGCGCATCTT CACAGTGGCC CAGATGGATG ACAACAGCAT CCAGATGAAG AAGGACCTGG 
     3721 CTGTCTTTCT GTACCATCTG CGCCTTGAGG CCGAGGTGGA GGTGGTGGAG ATGCATAACA 
     3781 GTGACATCTC TGCATACACC TACGAGCGGA CGCTGATGAT GGAGCAGCGG TCGCAGATGC 
     3841 TGCGGCAGAT GAGACTGACC AAGACTGAGC GGGAGCGAGA AGCCCAGCTG GTCAAGGATC 
     3901 GGCACTCGGC CCTGCGGCTG GAGAGCCTGT ACTCGGACGA GGAAGATGAG TCTGCAGTGG 
     3961 GGGCTGACAA GATCCAGATG ACGTGGACCA GGGACAAGTA CATGACTGAG ACCTGGGACC 
     4021 CCAGCCATGC CCCTGACAAT TTCCGGGAGC TGGTGCACAT TAAGCCGGAC CAATCCAATG 
     4081 TGCGGCGCAT GCACACTGCT GTGAAGCTCA ATGAAGTCAT TGTCACGCGC TCCCACGACG 
     4141 CCCGCCTGGT TCTCCTAAAC ATGCCTGGCC CACCCAGGAA CAGTGAGGGC GACGAGAACT 
     4201 ACATGGAGTT CCTCGAGGTG CTGACCGAGG GCCTTGAGCG GGTGCTGTTG GTGCGCGGTG 
     4261 GTGGCCGTGA AGTCATCACC ATCTACTCCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4321 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4381 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4441 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4501 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4561 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4621 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4681 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4741 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4801 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4861 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4921 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4981 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5041 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5101 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5161 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5221 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5281 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5341 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5401 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5461 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5521 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5581 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5641 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5701 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5761 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5821 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5881 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5941 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6001 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6061 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6121 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6181 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6241 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6301 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6361 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6421 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6481 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6541 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6601 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6661 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6721 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6781 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6841 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6901 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6961 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7021 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7081 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7141 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7201 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7261 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7321 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7381 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7441 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7501 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7561 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7621 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7681 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7741 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7801 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7861 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7921 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7981 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8041 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8101 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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