LOCUS       RC228636                8118 bp    DNA     circular  SYN 
DEFINITION  SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 2
ACCESSION   NM_001145961
VERSION     NM_001145961.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4275..4366 
                     /label="Myc-DDK"  
     misc_feature    4396..4981 
                     /label="PolyA signal"  
     misc_feature    4453..4472 
                     /label="Restriction Enzyme"  
     misc_feature    5257..5929 
                     /label="ColE1"  
     misc_feature    6359..7153 
                     /label="Kan/Neo"  
     misc_feature    7188..7490 
                     /label="SV40 ori"  
     misc_feature    7576..8053 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4257..4263 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4257 
                     /label="SLC12A4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCACTTC ACCGTGGTGC CAGTGGACGG GCCGAGGCGC GGCGACTATG 
     1081 ACAACCTCGA GGGGCTCAGT TGGGTGGACT ACGGGGAGCG CGCCGAGCTG GATGACTCGG 
     1141 ACGGACATGG CAACCACAGA GAGAGCAGCC CTTTTCTTTC CCCCTTGGAG GCTTCCAGAG 
     1201 GAATTGACTA CTATGACAGG AACCTGGCAC TGTTTGAGGA AGAGCTGGAC ATCCGCCCAA 
     1261 AGGTATCGTC TCTTCTGGGA AAGCTCGTCA GCTACACCAA CCTCACCCAG GGCGCCAAAG 
     1321 AGCATGAGGA GGCCGAGAGT GGGGAGGGCA CCCGCCGGAG GGCAGCCGAG GCACCCAGCA 
     1381 TGGGCACCCT CATGGGGGTG TACCTGCCCT GCCTGCAGAA TATCTTTGGG GTTATCCTCT 
     1441 TCCTGCGGCT GACCTGGATG GTGGGCACAG CAGGTGTGCT ACAGGCCCTC CTCATCGTGC 
     1501 TTATCTGCTG CTGTTGTACC CTGCTGACGG CCATCTCCAT GAGTGCCATC GCCACCAACG 
     1561 GTGTGGTTCC AGCTGGGGGC TCCTATTTCA TGATCTCTCG TTCACTGGGG CCAGAATTTG 
     1621 GAGGTGCTGT GGGCCTGTGC TTCTACCTGG GAACAACATT CGCAGCAGCC ATGTACATCC 
     1681 TGGGGGCCAT CGAGATCTTG CTGACCTACA TTGCCCCACC AGCTGCCATT TTTTACCCAT 
     1741 CGGGTGCTCA TGACACGTCG AATGCCACTT TGAACAATAT GCGTGTGTAT GGGACCATTT 
     1801 TCCTGACCTT CATGACCCTG GTGGTGTTTG TGGGGGTCAA GTATGTGAAC AAATTTGCCT 
     1861 CGCTCTTCCT GGCCTGTGTG ATCATCTCCA TCCTCTCCAT CTATGCTGGG GGCATAAAGT 
     1921 CTATATTTGA CCCTCCCGTG TTTCCGGTAT GCATGCTGGG CAACAGGACC CTGTCCCGGG 
     1981 ACCAGTTTGA CATCTGTGCC AAGACAGCTG TAGTGGACAA TGAGACAGTG GCCACCCAGC 
     2041 TATGGAGTTT CTTCTGCCAC AGCCCCAACC TTACGACCGA CTCCTGTGAC CCCTACTTCA 
     2101 TGCTCAACAA TGTGACCGAG ATCCCTGGCA TCCCCGGGGC AGCTGCTGGT GTGCTCCAGG 
     2161 AAAACCTGTG GAGCGCCTAC CTGGAGAAGG GTGACATCGT GGAGAAGCAT GGGCTGCCCT 
     2221 CCGCAGATGC CCCGAGCCTG AAGGAGAGCC TGCCTCTGTA CGTGGTCGCT GACATCGCCA 
     2281 CATCCTTCAC CGTGCTGGTC GGCATCTTCT TCCCTTCTGT AACAGGCATC ATGGCTGGCT 
     2341 CAAACCGCTC TGGGGACCTT CGTGACGCCC AGAAGTCTAT CCCTGTGGGG ACCATTCTGG 
     2401 CCATCATTAC AACTTCCCTC GTGTACTTCA GCAGTGTGGT TCTCTTTGGT GCCTGCATTG 
     2461 AGGGTGTGGT TCTCCGGGAC AAGTATGGCG ATGGTGTCAG CAGGAACTTG GTGGTGGGCA 
     2521 CACTGGCCTG GCCTTCACCC TGGGTCATCG TCATCGGCTC CTTCTTTTCA ACGTGTGGCG 
     2581 CTGGCCTCCA GAGCCTCACA GGGGCACCAC GCCTATTGCA GGCCATTGCC AAGGACAACA 
     2641 TCATCCCCTT CCTCCGGGTG TTTGGCCACG GGAAGGTGAA TGGTGAACCC ACATGGGCAC 
     2701 TCCTCCTGAC GGCACTCATC GCCGAGCTGG GCATCCTCAT CGCCTCCCTC GACATGGTGG 
     2761 CCCCCATCTT ATCCATGTTC TTTCTGATGT GCTACCTGTT CGTGAACCTC GCCTGTGCGG 
     2821 TGCAGACACT CCTGAGGACC CCCAACTGGC GGCCCCGGTT CAAGTACTAT CACTGGGCGC 
     2881 TGTCCTTCCT GGGCATGAGT CTCTGCCTGG CCCTTATGTT TGTCTCCTCC TGGTACTATG 
     2941 CCCTGGTGGC CATGCTCATC GCCGGCATGA TCTACAAATA CATCGAGTAC CAAGGGGCTG 
     3001 AGAAGGAGTG GGGTGACGGG ATCCGAGGCC TGTCCCTGAG CGCTGCCCGC TACGCGCTGT 
     3061 TGCGGCTGGA GGAGGGGCCT CCTCACACCA AGAACTGGCG GCCGCAGCTG CTGGTGCTGC 
     3121 TGAAGCTGGA CGAGGACCTC CACGTGAAGT ACCCGCGGCT CCTCACCTTC GCCTCCCAGC 
     3181 TCAAGGCTGG CAAGGGCCTG ACCATTGTTG GTTCTGTCAT CCAGGGGAGC TTCTTGGAGA 
     3241 GCTATGGCGA GGCTCAGGCC GCCGAGCAGA CCATCAAGAA CATGATGGAA ATTGAGAAGG 
     3301 TGAAGGGCTT CTGCCAGGTG GTGGTGGCCA GCAAGGTGCG GGAGGGGCTG GCCCACCTCA 
     3361 TCCAGTCCTG TGGCCTGGGA GGCATGCGGC ATAACTCCGT GGTGCTGGGC TGGCCCTACG 
     3421 GCTGGCGACA GAGCGAGGAC CCCCGTGCCT GGAAGACCTT CATTGACACC GTGCGCTGCA 
     3481 CTACGGCTGC CCACCTGGCC CTGCTCGTGC CCAAGAACAT CGCCTTCTAC CCCAGCAACC 
     3541 ACGAGCGCTA CCTGGAGGGC CACATAGACG TGTGGTGGAT CGTGCACGAT GGTGGCATGC 
     3601 TCATGCTTCT GCCCTTCCTG CTGCGCCAGC ATAAGGTCTG GAGGAAGTGC CGGATGCGCA 
     3661 TCTTCACAGT GGCCCAGATG GATGACAACA GCATCCAGAT GAAGAAGGAC CTGGCTGTCT 
     3721 TTCTGTACCA TCTGCGCCTT GAGGCCGAGC ATAACAGTGA CATCTCTGCA TACACCTACG 
     3781 AGCGGACGCT GATGATGGAG CAGCGGTCGC AGATGCTGCG GCAGATGAGA CTGACCAAGA 
     3841 CTGAGCGGGA GCGAGAAGCC CAGCTGGTCA AGGATCGGCA CTCGGCCCTG CGGCTGGAGA 
     3901 GCCTGTACTC GGACGAGGAA GATGAGTCTG CAGTGGGGGC TGACAAGATC CAGATGACGT 
     3961 GGACCAGGGA CAAGTACATG ACTGAGACCT GGGACCCCAG CCATGCCCCT GACAATTTCC 
     4021 GGGAGCTGGT GCACATTAAG CCGGACCAAT CCAATGTGCG GCGCATGCAC ACTGCTGTGA 
     4081 AGCTCAATGA AGTCATTGTC ACGCGCTCCC ACGACGCCCG CCTGGTTCTC CTAAACATGC 
     4141 CTGGCCCACC CAGGAACAGT GAGGGCGACG AGAACTACAT GGAGTTCCTC GAGGTGCTGA 
     4201 CCGAGGGCCT TGAGCGGGTG CTGTTGGTGC GCGGTGGTGG CCGTGAAGTC ATCACCATCT 
     4261 ACTCCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4321 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4381 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4441 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4501 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4561 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4621 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4681 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4741 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4801 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4861 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4921 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4981 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5041 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5101 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5161 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5221 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5281 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5341 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5401 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5461 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5521 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5581 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5641 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5701 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5761 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5821 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5881 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5941 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6001 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6061 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6121 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6181 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6241 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6301 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6361 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6421 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6481 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6541 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6601 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6661 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6721 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6781 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6841 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6901 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6961 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7021 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7081 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7141 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7201 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7261 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7321 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7381 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7441 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7501 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7561 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7621 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7681 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7741 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7801 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7861 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7921 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7981 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8041 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8101 AAATTTAACG CGAATTTT
//