LOCUS       RC228596                7443 bp    DNA     circular  SYN 
DEFINITION  PARP9 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 9 (PARP9), transcript variant 2
ACCESSION   NM_001146102
VERSION     NM_001146102.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3600..3691 
                     /label="Myc-DDK"  
     misc_feature    3721..4306 
                     /label="PolyA signal"  
     misc_feature    3778..3797 
                     /label="Restriction Enzyme"  
     misc_feature    4582..5254 
                     /label="ColE1"  
     misc_feature    5684..6478 
                     /label="Kan/Neo"  
     misc_feature    6513..6815 
                     /label="SV40 ori"  
     misc_feature    6901..7378 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3582..3588 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3582 
                     /label="PARP9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTTTTCC ATGGTGGCCG GAGCAGCAGC TTACAATGAA AAATCAGGTA 
     1081 GGATTACCTC GCTCTCACTC TTGTTTCAGA AAGTCTTTGC TCAGATCTTT CCTCAGTGGA 
     1141 GAAAGGGGAA TACAGAAGAA TGTCTCCCCT ACAAGTGCTC AGAGACTGGT GCTCTTGGAG 
     1201 AAAACTATAG TTGGCAAATT CCCATTAACC ACAATGACTT CAAAATTTTA AAAAATAATG 
     1261 AGCGTCAGCT GTGTGAAGTC CTCCAGAATA AGTTTGGCTG TATCTCTACC CTGGTCTCTC 
     1321 CAGTTCAGGA AGGCAACAGC AAATCTCTGC AAGTGTTCAG AAAAATGCTG ACTCCTAGGA 
     1381 TAGAGTTATC AGTCTGGAAA GATGACCTCA CCACACATGC TGTTGATGCT GTGGTGAATG 
     1441 CAGCCAATGA AGATCTTCTG CATGGGGGAG GCCTGGCCCT GGCCCTGGTA AAAGCTGGTG 
     1501 GATTTGAAAT CCAAGAAGAG AGCAAACAGT TTGTTGCCAG ATATGGTAAA GTGTCAGCTG 
     1561 GTGAGATAGC TGTCACGGGA GCAGGGAGGC TTCCCTGCAA ACAGATCATC CATGCTGTTG 
     1621 GGCCTCGGTG GATGGAATGG GATAAACAGG GATGTACTGG AAAGCTGCAG AGGGCCATTG 
     1681 TAAGTATTCT GAATTATGTC ATCTATAAAA ATACTCACAT TAAGACAGTA GCAATTCCAG 
     1741 CCTTGAGCTC TGGGATTTTT CAGTTCCCTC TGAATTTGTG TACAAAGACT ATTGTAGAGA 
     1801 CTATCCGGGT TAGTTTGCAA GGGAAGCCAA TGATGAGTAA TTTGAAAGAA ATTCACCTGG 
     1861 TGAGCAATGA GGACCCTACT GTTGCTGCCT TTAAAGCTGC TTCAGAATTC ATCCTAGGGA 
     1921 AGAGTGAGCT GGGACAAGAA ACCACCCCTT CTTTCAATGC AATGGTCGTG AACAACCTGA 
     1981 CCCTCCAGAT TGTCCAGGGC CACATTGAAT GGCAGACGGC AGATGTAATT GTTAATTCTG 
     2041 TAAACCCACA TGATATTACA GTTGGACCTG TGGCAAAGTC AATTCTACAA CAAGCAGGAG 
     2101 TTGAAATGAA ATCGGAATTT CTTGCCACAA AGGCTAAACA GTTTCAACGG TCCCAGTTGG 
     2161 TACTGGTCAC AAAAGGATTT AACTTGTTCT GTAAATATAT ATACCATGTA CTGTGGCATT 
     2221 CAGAATTTCC TAAACCTCAG ATATTAAAAC ATGCAATGAA GGAGTGTTTG GAAAAATGCA 
     2281 TTGAGCAAAA TATAACTTCC ATTTCCTTTC CTGCCCTTGG GACTGGAAAC ATGGAAATAA 
     2341 AGAAGGAAAC AGCAGCAGAG ATTTTGTTTG ATGAAGTTTT AACATTTGCC AAAGACCATG 
     2401 TAAAACACCA GTTAACTGTA AAATTTGTGA TCTTTCCAAC AGATTTGGAG ATATATAAGG 
     2461 CTTTCAGTTC TGAAATGGCA AAGAGGTCCA AGATGCTGAG TTTGAACAAT TACAGTGTCC 
     2521 CCCAGTCAAC CAGAGAGGAG AAAAGAGAAA ATGGGCTTGA AGCTAGATCT CCTGCCATCA 
     2581 ATCTGATGGG ATTCAACGTG GAAGAGATGT ATGAGGCCCA CGCATGGATC CAAAGAATCC 
     2641 TGAGTCTCCA GAACCACCAC ATCATTGAGA ATAATCATAT TCTGTACCTT GGGAGAAAGG 
     2701 AACATGACAT TTTGTCTCAG CTTCAGAAAA CTTCAAGTGT CTCCATCACA GAAATTATCA 
     2761 GCCCAGGAAG GACAGAGTTA GAGATTGAAG GAGCCCGGGC TGACCTCATT GAGGTGGTTA 
     2821 TGAACATTGA AGATATGCTT TGTAAAGTAC AGGAGGAAAT GGCAAGGAAA AAGGAGCGAG 
     2881 GCCTTTGGCG CTCGTTAGGA CAGTGGACTA TTCAGCAACA AAAAACCCAA GACGAAATGA 
     2941 AAGAAAATAT CATATTTCTG AAATGTCCTG TGCCTCCAAC TCAAGAGCTT CTAGATCAAA 
     3001 AGAAACAGTT TGAAAAATGT GGTTTGCAGG TTCTAAAGGT GGAGAAGATA GACAATGAGG 
     3061 TCCTTATGGC TGCCTTTCAA AGAAAGAAGA AAATGATGGA AGAAAAACTG CACAGGCAAC 
     3121 CTGTGAGCCA TAGGCTGTTT CAGCAAGTCC CATACCAGTT CTGCAATGTG GTATGCAGAG 
     3181 TTGGCTTTCA AAGAATGTAC TCGACACCTT GCGATCCAAA ATACGGAGCT GGCATATACT 
     3241 TCACCAAGAA CCTCAAAAAC CTGGCAGAGA AGGCCAAGAA AATCTCTGCT GCAGATAAGC 
     3301 TGATCTATGT GTTTGAGGCT GAAGTACTCA CAGGCTTCTT CTGCCAGGGA CATCCGTTAA 
     3361 ATATTGTTCC CCCACCACTG AGTCCTGGAG CTATAGATGG TCATGACAGT GTGGTTGACA 
     3421 ATGTCTCCAG CCCTGAAACC TTTGTTATTT TTAGTGGCAT GCAGGCTATA CCTCAGTATT 
     3481 TGTGGACATG CACCCAGGAA TATGTACAGT CACAAGATTA CTCATCAGGA CCAATGAGAC 
     3541 CCTTTGCACA GCATCCTTGG AGGGGATTCG CAAGTGGCAG CCCTGTTGAT ACGCGTACGC 
     3601 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3661 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3721 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3781 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3841 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3901 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3961 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4021 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4081 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4141 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4201 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4261 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4321 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4381 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4441 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4501 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4561 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4621 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4681 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4741 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4801 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4861 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4921 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4981 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5041 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5101 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5161 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5221 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5281 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5341 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5401 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5461 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5521 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5581 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5641 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5701 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5761 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5821 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5881 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5941 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6001 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6061 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6121 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6181 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6241 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6301 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6361 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6421 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6481 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6541 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6601 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6661 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6721 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6781 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6841 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6901 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6961 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7021 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7081 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7141 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7201 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7261 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7321 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7381 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7441 TTT
//