LOCUS       RC228581                7239 bp    DNA     circular  SYN 
DEFINITION  ELAC2 (Myc-DDK-tagged)-Human elaC homolog 2 (E. coli) (ELAC2), transcript variant 3
ACCESSION   NM_001165962
VERSION     NM_001165962.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3396..3487 
                     /label="Myc-DDK"  
     misc_feature    3517..4102 
                     /label="PolyA signal"  
     misc_feature    3574..3593 
                     /label="Restriction Enzyme"  
     misc_feature    4378..5050 
                     /label="ColE1"  
     misc_feature    5480..6274 
                     /label="Kan/Neo"  
     misc_feature    6309..6611 
                     /label="SV40 ori"  
     misc_feature    6697..7174 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3378..3384 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3378 
                     /label="ELAC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGGCGCTT TGCTCGCTGC TGCGGTCCGC GGCCGGACGC ACCATGTCGC 
     1081 AGGGACGCAC CATATCGCAG GCACCCGCCC GCCGCGAGCG GCCGCGCAAG GACCCGCTGC 
     1141 GGCACCTGCG CACGCGAGAG AAGCGCGGAC CGTCGGGGTG CTCCGGCGGC CCAAACACCG 
     1201 TGTACCTGCA GGTGGTGGCA GCGGGTAGCC GGGACTCGGG CGCCGCGCTC TACGTCTTCT 
     1261 CCGAGTTCAA CCGGTATCTC TTCAACTGTG GAGAAGGCGT TCAGAGACTC ATGCAGGAGC 
     1321 ACAAGTTAAA GGTTGCTCGC CTGGACAACA TATTCCTGAC ACGAATGCAC TGGTCTAATG 
     1381 TTGGGGGCTT AAGTGGAATG ATTCTTACTT TAAAGGAAAC CGGGCTTCCA AAGTGTGTAC 
     1441 TTTCTGGACC TCCACAACTG GAAAAATACC TCGAAGCAAT CAAAATATTT TCTGGTCCAT 
     1501 TGAAAGGAAT AGAACTGGCT GTGCGGCCCC ACTCTGCCCC AGAATACGAG GATGAAACCA 
     1561 TGACAGTTTA CCAGATCCCC ATACACAGTG TTAGCCAGAG AAGAGGGGTC AGGGACTCTT 
     1621 CCCTGGTCGT AGCTTTCATC TGTAAGCTTC ACTTAAAGAG AGGAAACTTC TTGGTGCTCA 
     1681 AAGCAAAGGA GATGGGCCTC CCAGTTGGGA CAGCTGCCAT CGCTCCCATC ATTGCTGCTG 
     1741 TCAAGGACGG GAAAAGCATC ACTCATGAAG GAAGAGAGAT TTTGGCTGAA GAGCTGTGTA 
     1801 CTCCTCCAGA TCCTGGTGCT GCTTTTGTGG TGGTAGAATG TCCAGATGAA AGCTTCATTC 
     1861 AACCCATCTG TGAGAATGCC ACCTTTCAGA GGTACCAAGG AAAGGCAGAT GCCCCCGTGG 
     1921 CCTTGGTGGT TCACATGGCC CCAGCATCTG TGCTTGTGGA CAGCAGGTAC CAGCAGTGGA 
     1981 TGGAGAGGTT TGGGCCTGAC ACCCAGCACT TGGTCCTGAA TGAGAACTGT GCCTCAGTTC 
     2041 ACAACCTTCG CAGCCACAAG ATTCAAACCC AGCTCAACCT CATCCACCCG GACATCTTCC 
     2101 CCCTGCTCAC CAGTTTCCGC TGTAAGAAGG AGGGCCCCAC CCTCAGTGTG CCCATGGTTC 
     2161 AGGGTGAATG CCTCCTCAAG TACCAGCTCC GTCCCAGGAG GGAGTGGCAG AGGGATGCCA 
     2221 TTATTACTTG CAATCCTGAG GAATTCATAG TTGAGGCGCT GCAGCTTCCC AACTTCCAGC 
     2281 AGAGCGTGCA GGAGTACAGG AGGAGTGCGC AGGACGGCCC AGCCCCAGCA GAGAAAAGAA 
     2341 GTCAGTACCC AGAAATCATC TTCCTTGGAA CAGGGTCTGC CATCCCGATG AAGATTCGAA 
     2401 ATGTCAGTGC CACACTTGTC AACATAAGCC CCGACACGTC TCTGCTACTG GACTGTGGTG 
     2461 AGGGCACATT TGGGCAGCTG TGCCGTCATT ACGGAGACCA GGTGGACAGG GTCCTGGGCA 
     2521 CCCTGGCTGC TGTGTTTGTG TCCCACCTGC ACGCAGATCA CCACACGGGC TTGCCAAGTA 
     2581 TCTTGCTGCA GAGAGAACGC GCCTTGGCAT CTTTGGGAAA GCCGCTTCAC CCTTTGCTGG 
     2641 TGGTTGCCCC CAACCAGCTC AAAGCCTGGC TCCAGCAGTA CCACAACCAG TGCCAGGAGG 
     2701 TCCTGCACCA CATCAGTATG ATTCCTGCCA AATGCCTTCA GGAAGGGGCT GAGATCTCCA 
     2761 GTCCTGCAGT GGAAAGATTG ATCAGTTCGC TGTTGCGAAC ATGTGATTTG GAAGAGTTTC 
     2821 AGACCTGTCT GGTGCGGCAC TGCAAGCATG CGTTTGGCTG TGCGCTGGTG CACACCTCTG 
     2881 GCTGGAAAGT GGTCTATTCC GGGGACACCA TGCCCTGCGA GGCTCTGGTC CGGATGGGGA 
     2941 AAGATGCCAC CCTCCTGATA CATGAAGCCA CCCTGGAAGA TGGTTTGGAA GAGGAAGCAG 
     3001 TGGAAAAGAC ACACAGCACA ACGTCCCAAG CCATCAGCGT GGGGATGCGG ATGAACGCGG 
     3061 AGTTCATTAT GCTGAACCAC TTCAGCCAGC GCTATGCCAA GGTCCCCCTC TTCAGCCCCA 
     3121 ACTTCAGCGA GAAAGTGGGA GTTGCCTTTG ACCACATGAA GGTCTGCTTT GGAGACTTTC 
     3181 CAACAATGCC CAAGCTGATT CCCCCACTGA AAGCCCTGTT TGCTGGCGAC ATCGAGGAGA 
     3241 TGGAGGAGCG CAGGGAGAAG CGGGAGCTGC GGCAGGTGCG GGCGGCCCTC CTGTCCAGGG 
     3301 AGCTGGCAGG CGGCCTGGAG GATGGGGAGC CTCAGCAGAA GCGGGCCCAC ACAGAGGAGC 
     3361 CACAGGCCAA GAAGGTCAGA GCCCAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3421 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3481 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3541 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3601 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3661 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3721 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3781 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3841 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3901 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3961 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4021 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4081 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4141 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4201 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4261 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4321 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4381 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4441 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4501 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4561 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4621 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4681 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4741 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4801 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4861 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4921 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4981 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5041 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5101 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5161 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5221 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5281 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5341 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5401 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5461 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5521 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5581 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5641 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5701 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5761 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5821 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5881 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5941 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6001 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6061 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6121 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6181 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6241 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6301 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6361 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6421 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6481 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6541 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6601 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6661 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6721 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6781 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6841 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6901 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6961 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7021 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7081 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7141 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7201 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//