LOCUS       RC228558                7071 bp    DNA     circular  SYN 
DEFINITION  SYTL5 (Myc-DDK-tagged)-Human synaptotagmin-like 5 (SYTL5), transcript variant 2
ACCESSION   NM_001163335
VERSION     NM_001163335.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3228..3319 
                     /label="Myc-DDK"  
     misc_feature    3349..3934 
                     /label="PolyA signal"  
     misc_feature    3406..3425 
                     /label="Restriction Enzyme"  
     misc_feature    4210..4882 
                     /label="ColE1"  
     misc_feature    5312..6106 
                     /label="Kan/Neo"  
     misc_feature    6141..6443 
                     /label="SV40 ori"  
     misc_feature    6529..7006 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3210..3216 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3210 
                     /label="SYTL5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTAAGAAC TCAGAGTTCA TCAATCTGTC ATTTTTATTA GATCATGAGA 
     1081 AGGAAATGAT CCTGGGCGTC CTAAAGAGAG ATGAATATTT GAAAAAAGTG GAGGACAAGA 
     1141 GAATAAGGAA GCTGAAAAAT GAACTCTTAG AAGCAAAACG TAGAAGTGGG AAAACTCAAC 
     1201 AAGAGGCCAG CAGAGTTTGT GTTCACTGTC ACAGAAACCT GGGCCTAATC TTTGACCGGG 
     1261 GAGACCCTTG TCAGGCTTGC TCACTGAGGG TATGCAGGGA GTGTCGAGTT GCAGGCCCCA 
     1321 ATGGCAGCTG GAAGTGCACT GTCTGTGACA AAATCGCGCA GCTAAGGATT ATAACTGGTG 
     1381 AGTGGTTTTT TGAAGAAAAG GCAAAACGTT TCAAGCAAGT CAATGTTCTC GGCACTGATG 
     1441 TTGTCCGACA GTCCATTTTA AGAAGAAGTC CAGGAGCTGA AGAAGTACAG AGCCAAGAGC 
     1501 AAACCCGCCA GGATGCAGAA AAGTCAGACA CTTCACCTGT TGCTGGGAAG AAGGCCAGCC 
     1561 ATGATGGGCC CAAGAGAAAG GGATTTCTTC TTAGCAAGTT CAGATCGGCA ACCAGAGGAG 
     1621 AAATCATAAC TCCCAAAACT GACACTGGGC GGAGCTATAG CTTGGACTTA GACGGTCAAC 
     1681 ATTTTCGGAG TTTAAAATCA CCTCCTGGTT CAGACAGGGG AAGCACTGGC TCATCAGATC 
     1741 TCAATGACCA GGAACCTGGT CCTAGGACCC CGAAGAGCAG TCGGAGCAAT GGTGTGACCC 
     1801 CAGGCACTCA GAGTTCACCA GCCCCAAGCA CACGAACTGT GACCTCAGTC ATCAGTAGAG 
     1861 AGTATGGTTT TGAAAATTCC ATGGATTTGG CTGCTATTGA AGGTACCTCT CAGGAGCTCA 
     1921 CAAAGAGTCA CCGCAGAAAC ACTTCTGGCA CACCTTCCAT AGCAGTGTCT GGAACCTCTC 
     1981 TCTCCTCAGA TCAGAGTCGA TCTGAGTTAG ATTTGAGTGA GTCATTTACA GAAGACTCAG 
     2041 AGGATACTGT AAGCATAAGA AGCAAGTCTG TCCCTGGGGC TTTAGACAAG GACTCCTTGG 
     2101 AAGAGACTGA AGAAAGCATT GATGCCTTAG TGTCCTCGCA GTTATCTACA AACACTCACC 
     2161 GTCTGGCAAG TGGCCTATCA ACTACCAGCC TTAACAGCAT GATGAGCGTT TACAGTGAAA 
     2221 CGGGAGACTA TGGCAACGTG AAAGTCAGTG GTGAAATCCT TCTCCATATC AGCTACTGCT 
     2281 ACAAAACTGG TGGGCTGTAC ATTTTTGTCA AGAATTGCAG AAATCTGGCC ATAGGAGATG 
     2341 AAAAGAAACA GAGGACAGAT GCTTATGTCA AGTCATATCT TCTTCCTGAC AAGTCCCGGA 
     2401 ACAACAAGCG TAAGACCAAA ATCAGAACAG GCACCAATCC AGAATTCAAT GAAACACTAA 
     2461 AGTACACTAT CAGCCATACC CAGCTGGAAA CAAGAACTCT GCAGCTCTCA GTCTGGCACT 
     2521 ATGATCGATT TGGACGTAAT AGCTTCCTCG GGGAAGTAGA GATTCCTTTT GACTCATGGA 
     2581 ACTTTGAAAA TCCAACTGAT GAGTGGTTTG TGCTTCAACC CAAGGTGGAG TTTGCTCCTG 
     2641 ATATTGGCCT TCAATACAAA GGAGAGCTGA CAGTTGTTTT ACGTTACATT CCCCCAGAAG 
     2701 AGAACCTGAT GCTTCCACCA GAACAACTCC AAGGAAATAA GACTTTTAAA AAGGGAAAGA 
     2761 AGAAGGAGTC ACCTGTAATC TCTGGAGGAA TACTAGAAGT GTTCATCAAA GAGGCAAAGA 
     2821 ATTTGACAGC AGTGAAGTCA GGAGGCACTT CTGATAGCTT TGTGAAGGGC TACCTGCTCC 
     2881 CTGATGATAG CAAAGCCACC AAGCACAAAA CTCTGGTAAT AAAAAAGAGT GTTAACCCTC 
     2941 AGTGGAATCA TACATTCATG TTCAGTGGCA TCCATCCCCA GGATATAAAG AATGTTTGCC 
     3001 TAGAACTTAC TATCTGGGAC AAGGAGGCCT TTTCCAGCAA CATCTTTCTG GGAGGAGTTC 
     3061 GTTTGAATTC TGGAAGTGGT GTGAGCCATG GGAAGAACGT GGATTGGATG GACTCTCAGG 
     3121 GGGAAGAGCA GCGCCTTTGG CAGAAGATGG CCAACAACCC TGGAACTCCC TTTGAGGGTG 
     3181 TACTCATGCT TCGTTCCAGC ATGGGAAAAT GTAGGCTCAC GCGTACGCGG CCGCTCGAGC 
     3241 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3301 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3361 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3421 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3481 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3541 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3601 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3661 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3721 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3781 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3841 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3901 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3961 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4021 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4081 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4141 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4201 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4261 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4321 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4381 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4441 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4501 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4561 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4621 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4681 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4741 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4801 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4861 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4921 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4981 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5041 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5101 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5161 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5221 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5281 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5341 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5401 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5461 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5521 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5581 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5641 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5701 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5761 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5821 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5881 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5941 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6001 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6061 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6121 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6181 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6241 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6301 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6361 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6421 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6481 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6541 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6601 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6661 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6721 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6781 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6841 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6901 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6961 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7021 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//