LOCUS       RC227939                8841 bp    DNA     circular  SYN 
DEFINITION  TMEM2 (Myc-DDK-tagged)-Human transmembrane protein 2 (TMEM2), transcript variant 2
ACCESSION   NM_001135820
VERSION     NM_001135820.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4998..5089 
                     /label="Myc-DDK"  
     misc_feature    5119..5704 
                     /label="PolyA signal"  
     misc_feature    5176..5195 
                     /label="Restriction Enzyme"  
     misc_feature    5980..6652 
                     /label="ColE1"  
     misc_feature    7082..7876 
                     /label="Kan/Neo"  
     misc_feature    7911..8213 
                     /label="SV40 ori"  
     misc_feature    8299..8776 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4980..4986 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4980 
                     /label="TMEM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTATGCCACT GATTCCAGGG GACACTCCCC TGCTTTCCTC CAACCTCAGA 
     1081 ATGGAAATAG TCGTCACCCA TCTGGCTATG TTCCAGGGAA GGTTGTCCCA TTGCGTCCCC 
     1141 CTCCTCCTCC AAAGAGTCAA GCTTCAGCCA AATTTACCTC CATCAGACGA GAAGACCGGG 
     1201 CAACCTTCGC ATTCTCACCT GAAGAACAGC AAGCCCAGAG AGAAAGTCAA AAGCAAAAGA 
     1261 GACACAAAAA TACTTTCATT TGTTTTGCTA TTACTAGTTT CTCATTTTTT ATTGCACTTG 
     1321 CAATCATTTT AGGAATATCC TCAAAATATG CTCCAGATGA AAATTGCCCA GATCAAAATC 
     1381 CTCGTCTCAG GAATTGGGAT CCAGGACAAG ATTCTGCAAA GCAAGTTGTT ATCAAGGAGG 
     1441 GAGATATGCT CCGTCTGACC TCAGACGCCA CCGTGCATTC TATAGTCATT CAGGATGGAG 
     1501 GACTGCTTGT ATTTGGGGAC AATAAAGATG GATCCAGAAA TATTACTTTG AGGACTCATT 
     1561 ACATCCTGAT CCAGGATGGT GGGGCGCTTC ATATTGGAGC AGAAAAATGC CGCTATAAAT 
     1621 CCAAAGCGAC AATTACCTTG TATGGCAAGT CAGATGAAGG TGAAAGTATG CCAACATTTG 
     1681 GCAAAAAGTT TATTGGTGTG GAAGCTGGCG GGACACTGGA GTTACATGGG GCACGGAAGG 
     1741 CATCGTGGAC GTTGTTGGCA AGGACCCTGA ATTCCTCAGG CTTGCCCTTT GGGTCCTATA 
     1801 CCTTTGAAAA GGACTTTTCC CGGGGCCTCA ATGTGAGGGT CATTGACCAA GACACGGCCA 
     1861 AAATTTTGGA AAGTGAGAGA TTTGATACCC ATGAATACCG CAATGAGAGC AGGCGGCTTC 
     1921 AGGAGTTTCT GAGATTCCAG GATCCAGGTC GGATTGTTGC CATAGCTGTC GGGGATTCAG 
     1981 CCGCTAAAAG TCTCTTACAA GGAACCATCC AGATGATCCA GGAACGGTTG GGAAGTGAAC 
     2041 TGATCCAAGG ACTGGGCTAC AGGCAAGCTT GGGCTTTAGT TGGTGTCATT GATGGTGGAA 
     2101 GCACTTCTTG CAATGAATCC GTGAGAAACT ATGAAAATCA TAGCAGTGGC GGGAAGGCTC 
     2161 TTGCCCAAAG AGAATTTTAT ACTGTGGATG GCCAGAAGTT CTCTGTGACA GCTTATAGTG 
     2221 AATGGATTGA AGAAACCCCT CAGTTCCTGC ACATGGGTGA GATCATAGAC GGTGTAGACA 
     2281 TGAGAGCTGA GGTTGGAATT CTTACCCGGA ATATTGTGAT CCAAGGAGAA GTGGAGGACT 
     2341 CATGCTACGC AGAAAATCAG TGCCAATTTT TTGATTATGA TACCTTTGGG GGACACATTA 
     2401 TGATAATGAA AAATTTTACT TCAGTCCATC TTTCTTATGT GGAATTGAAA CACATGGGTC 
     2461 AGCAGCAGAT GGGGCGATAC CCTGTTCATT TTCACCTGTG TGGTGACGTG GATTATAAAG 
     2521 GAGGATACAG ACATGCAACA TTTGTGGACG GCCTGTCTAT TCATCACAGC TTCTCAAGGT 
     2581 GCATCACTGT GCATGGGACA AATGGCTTGC TAATAAAAGA CACCATTGGG TTTGACACAC 
     2641 TAGGTCATTG TTTCTTTTTG GAAGATGGTA TTGAACAGAG GAATACTTTG TTCCACAATC 
     2701 TGGGACTCCT CACCAAGCCG GGTACTCTCC TGCCCACCGA TAGGAACAAC TCCATGTGTA 
     2761 CCACCATGCG AGATAAAGTG TTTGGAAATT ACATTCCTGT GCCTGCTACT GACTGTATGG 
     2821 CTGTTTCAAC TTTCTGGATT GCTCATCCCA ACAATAATCT GATTAATAAT GCAGCTGCAG 
     2881 GCTCACAGGA TGCTGGAATA TGGTATTTAT TCCACAAGGA ACCAACTGGG GAATCCAGTG 
     2941 GATTGCAGCT CTTGGCAAAA CCAGAACTCA CTCCATTGGG TATATTTTAT AACAACAGGG 
     3001 TCCATTCAAA TTTTAAGGCT GGCTTATTTA TTGACAAAGG TGTCAAAACA ACCAACTCTA 
     3061 GTGCTGCTGA CCCAAGGGAA TACCTCTGTT TGGACAATAG TGCAAGATTT CGACCTCATC 
     3121 AGGATGCAAA CCCCGAAAAA CCACGTGTTG CTGCTCTAAT TGACAGGCTC ATTGCTTTTA 
     3181 AAAATAATGA TAATGGAGCT TGGGTCAGAG GAGGAGATAT TATCGTTCAA AATTCAGCAT 
     3241 TTGCAGATAA TGGAATAGGA CTGACCTTTG CCAGTGATGG AAGCTTCCCA AGTGATGAAG 
     3301 GTTCCAGCCA AGAGGTATCT GAATCTCTCT TTGTTGGGGA GAGCAGGAAT TACGGCTTTC 
     3361 AGGGTGGTCA GAACAAGTAT GTAGGCACTG GAGGAATAGA CCAGAAGCCT CGAACATTAC 
     3421 CCAGGAACAG GACGTTCCCA ATTAGAGGCT TTCAGATTTA TGATGGGCCC ATTCATCTCA 
     3481 CAAGGAGCAC TTTCAAAAAA TATGTGCCAA CTCCAGATAG GTACAGCAGT GCAATTGGCT 
     3541 TCCTCATGAA GAATTCCTGG CAGATAACCC CCAGGAATAA TATCTCCCTC GTGAAGTTTG 
     3601 GTCCACATGT CTCTCTGAAT GTCTTTTTTG GAAAGCCTGG TCCCTGGTTT GAAGATTGTG 
     3661 AGATGGATGG TGATAAGAAC TCCATATTCC ATGACATTGA TGGCTCTGTG ACAGGATACA 
     3721 AGGATGCTTA TGTGGGAAGA ATGGACAACT ACCTGATCCG CCATCCAAGC TGTGTAAATG 
     3781 TGTCTAAGTG GAATGCAGTG ATCTGCAGTG GGACCTATGC ACAGGTCTAT GTACAGACAT 
     3841 GGAGCACTCA GAATCTTTCT ATGACCATTA CACGAGATGA GTATCCGTCC AACCCTATGG 
     3901 TGCTCCGAGG TATTAATCAG AAGGCTGCCT TTCCACAGTA CCAGCCTGTC GTCATGCTGG 
     3961 AGAAGGGTTA TACCATCCAC TGGAATGGGC CGGCACCACG GACTACATTT CTATACCTCG 
     4021 TCAACTTCAA CAAGAATGAC TGGATTCGAG TTGGCCTTTG CTATCCATCA AACACAAGTT 
     4081 TTCAAGTTAC CTTTGGCTAT TTGCAGCGGC AGAATGGCTC ATTATCCAAA ATCGAAGAAT 
     4141 ATGAGCCTGT GCATTCACTG GAAGAACTGC AAAGAAAGCA ATCCGAGAGG AAATTCTATT 
     4201 TTGACTCCAG CACGGGGTTA CTGTTTTTGT ATCTCAAAGC CAAAAGCCAC AGGCATGGCC 
     4261 ACAGTTACTG TTCATCTCAG GGATGTGAAA GAGTCAAGAT CCAAGCAGCC ACAGACTCAA 
     4321 AGGACATCAG TAACTGCATG GCCAAAGCAT ACCCACAGTA CTACAGAAAG CCGTCAGTGG 
     4381 TCAAGCGGAT GCCGGCCATG CTCACTGGAC TCTGTCAAGG CTGTGGCACT CGGCAGGTGG 
     4441 TGTTTACTAG TGATCCTCAT AAAAGTTACC TCCCTGTGCA ATTCCAGTCA CCTGATAAAG 
     4501 CAGAAACCCA GCGTGGAGAC CCGTCTGTTA TTTCTGTCAA TGGCACTGAC TTTACCTTCC 
     4561 GAAGTGCAGG CGTCCTCCTC CTTGTTGTGG ATCCGTGCAG CGTTCCATTC CGCTTGACGG 
     4621 AAAAAACGGT TTTTCCTCTT GCTGATGTCA GTCGCATTGA AGAGTATTTA AAAACAGGCA 
     4681 TCCCTCCAAG GTCCATTGTT CTGTTGAGCA CAAGAGGAGA AATAAAGCAG TTAAACATTT 
     4741 CACACTTACT AGTACCTCTG GGATTAGCCA AACCAGCTCA TCTTTATGAC AAAGGGAGTA 
     4801 CCATATTTTT GGGATTCAGT GGAAACTTTA AACCATCATG GACTAAGCTA TTTACCAGTC 
     4861 CTGCTGGACA GGGCCTTGGG GTGCTTGAAC AATTCATACC TTTGCAGCTG GACGAATATG 
     4921 GTTGTCCCAG AGCCACCACT GTCCGCAGAA GAGACCTGGA ACTGCTAAAG CAAGCTTCAA 
     4981 AAGCACATAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5041 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5101 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5161 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5221 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5281 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5341 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5401 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5461 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5521 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5581 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5641 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5701 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5761 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5821 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5881 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5941 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6001 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6061 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6121 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6181 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6241 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6301 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6361 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6421 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6481 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6541 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6601 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6661 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6721 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6781 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6841 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6901 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6961 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7021 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7081 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7141 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7201 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7261 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7321 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7381 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7441 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7501 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7561 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7621 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7681 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7741 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7801 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7861 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7921 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7981 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8041 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8221 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8281 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8341 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8401 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8461 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8521 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8581 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8641 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8701 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8761 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8821 CAAAAATTTA ACGCGAATTT T
//