LOCUS       RC227932                8241 bp    DNA     circular  SYN 
DEFINITION  CASKIN2 (Myc-DDK-tagged)-Human CASK interacting protein 2 (CASKIN2), transcript variant 2
ACCESSION   NM_001142643
VERSION     NM_001142643.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4398..4489 
                     /label="Myc-DDK"  
     misc_feature    4519..5104 
                     /label="PolyA signal"  
     misc_feature    4576..4595 
                     /label="Restriction Enzyme"  
     misc_feature    5380..6052 
                     /label="ColE1"  
     misc_feature    6482..7276 
                     /label="Kan/Neo"  
     misc_feature    7311..7613 
                     /label="SV40 ori"  
     misc_feature    7699..8176 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4380..4386 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4380 
                     /label="CASKIN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCCCGCTG CACTACGCAG CCTGGCAGGG CCGGCTGGAG CCTGTGAGGC 
     1081 TGCTGCTGCG CGCCTCTGCG GCTGTCAATG CCGCCTCGCT GGACGGACAG ATCCCCCTGC 
     1141 ACCTGGCTGC ACAGTATGGA CATTATGAGG TGTCAGAAAT GCTCCTCCAG CATCAGTCCA 
     1201 ACCCATGCCT GGTCAACAAG GCCAAGAAGA CGCCCCTGGA CCTGGCCTGT GAATTTGGCC 
     1261 GACTCAAGGT GGCCCAGCTG CTTCTGAACA GCCACTTATG TGTGGCACTG CTGGAGGGTG 
     1321 AGGCCAAAGA CCCGTGTGAC CCCAACTACA CCACGCCCCT GCACTTGGCT GCCAAGAATG 
     1381 GCCACAGAGA AGTCATCAGG CAGCTCCTGA GAGCTGGGAT CGAGATCAAC CGCCAGACCA 
     1441 AGACGGGTAC GGCGCTCCAC GAGGCCGCAC TGTATGGCAA GACCGAGGTG GTGCGGCTGC 
     1501 TTCTGGAGGG AGGTGTGGAC GTGAACATCC GGAATACGTA TAACCAGACG GCGCTGGACA 
     1561 TAGTGAATCA GTTCACCACC TCCCAGGCCA GCCGGGAAAT CAAGCAGCTA CTGCGGGAGG 
     1621 CCTCAGGGAT CCTGAAGGTC CGAGCGCTCA AGGATTTCTG GAACCTCCAC GATCCCACTG 
     1681 CTCTCAATGT CCGGGCAGGG GATGTCATCA CGGTGCTAGA ACAGCATCCC GACGGCCGCT 
     1741 GGAAGGGCCA CATCCACGAG AGCCAGAGGG GCACAGACCG CATAGGCTAC TTCCCCCCGG 
     1801 GCATTGTCGA GGTGGTCAGC AAGCGGGTGG GCATCCCTGC AGCCCGCCTC CCCTCCGCAC 
     1861 CCACCCCCCT GCGCCCAGGC TTCTCCCGGA CACCGCAGCC TCCTGCCGAA GAACCCCCGC 
     1921 ACCCTCTTAC CTACAGCCAG CTTCCTCGGG TGGGCCTCAG CCCAGACAGC CCAGCAGGTG 
     1981 ACAGGAATAG TGTGGGCAGT GAGGGCAGCG TGGGCAGCAT CCGCAGTGCC GGCAGCGGGC 
     2041 AGAGCTCTGA GGGCACTAAT GGCCATGGCC CTGGCCTCCT GATTGAGAAC GCCCAGCCAC 
     2101 TGCCCTCTGC TGGAGAGGAC CAGGTGCTGC CAGGACTCCA CCCGCCGTCC CTGGCAGACA 
     2161 ACCTGAGCCA CCGCCCTCTG GCCAACTGCC GCTCTGGGGA GCAGATCTTC ACCCAGGACG 
     2221 TGCGGCCAGA ACAGCTGCTG GAGGGGAAGG ACGCGCAGGC CATTCATAAC TGGCTAAGCG 
     2281 AGTTCCAGCT GGAGGGCTAC ACTGCCCACT TTCTGCAGGC CGGCTATGAT GTGCCTACCA 
     2341 TCAGCCGCAT GACACCTGAG GACCTGACGG CCATCGGGGT GACCAAGCCT GGGCACAGGA 
     2401 AGAAGATCGC CTCAGAGATC GCTCAGCTCA GCATCGCCGA GTGGCTGCCC AGCTACATCC 
     2461 CAACGGACCT GCTGGAGTGG CTGTGTGCAC TGGGGCTGCC ACAGTACCAC AAGCAGCTGG 
     2521 TGAGCAGCGG CTACGACTCC ATGGGGCTGG TGGCCGACCT CACCTGGGAG GAGCTGCAGG 
     2581 AGATTGGGGT CAACAAGCTC GGGCATCAGA AGAAGCTCAT GCTGGGGGTG AAGCGGCTGG 
     2641 CGGAGCTTCG GCGGGGCCTG CTGCAGGGGG AGGCCCTCAG CGAAGGCGGG CGCCGGCTGG 
     2701 CCAAGGGTCC GGAGCTGATG GCCATCGAGG GACTGGAGAA CGGAGAAGGC CCAGCTACAG 
     2761 CTGGCCCACG GCTCCTCACC TTCCAGGGCA GCGAACTAAG CCCAGAGCTA CAGGCGGCCA 
     2821 TGGCAGGGGG TGGCCCTGAA CCACTCCCCC TCCCACCTGC CCGCTCTCCC AGCCAGGAGA 
     2881 GCATCGGGGC ACGCTCACGG GGGTCTGGCC ACTCACAGGA ACAGCCTGCC CCACAGCCCA 
     2941 GCGGTGGAGA TCCCAGCCCC CCCCAGGAGA GGAACCTCCC AGAGGGCACA GAGCGGCCCC 
     3001 CTAAGCTTTG TTCTTCACTT CCTGGCCAAG GACCCCCACC CTATGTTTTT ATGTACCCCC 
     3061 AGGGCTCACC CTCTAGCCCG GCCCCAGGGC CACCTCCTGG CGCACCCTGG GCCTTCTCCT 
     3121 ACTTGGCCGG GCCCCCTGCC ACTCCCCCAG ACCCGCCTCG ACCTAAGCGC CGGTCCCACA 
     3181 GCCTAAGCCG CCCTGGCCCC ACAGAGGGGG ATGCTGAGGG GGAGGCCGAA GGGCCAGTGG 
     3241 GCAGCACCCT AGGCAGTTAT GCTACCCTTA CCCGGCGGCC AGGACGCAGT GCCCTTGTCC 
     3301 GGACCAGTCC TAGTGTGACC CCAACCCCAG CTCGGGGGAC TCCTCGCAGC CAGTCCTTTG 
     3361 CCCTGCGGGC CCGGCGCAAA GGCCCCCCGC CCCCGCCCCC CAAGCGCCTC AGCTCCGTCT 
     3421 CTGGCCCCAG CCCGGAGCCA CCTCCACTAG ATGAGAGCCC AGGGCCCAAG GAAGGGGCCA 
     3481 CAGGGCCCCG AAGGCGAACA CTGAGTGAAC CTGCTGGCCC CTCAGAGCCC CCTGGCCCAC 
     3541 CTGCCCCGGC TGGGCCCGCG TCAGACACGG AGGAGGAGGA GCCAGGCCCT GAGGGGACGC 
     3601 CCCCATCTCG GGGCAGCTCT GGGGAAGGGC TGCCGTTTGC AGAGGAAGGG AACCTGACCA 
     3661 TCAAACAGCG CCCGAAGCCC GCTGGCCCCC CGCCCCGAGA GACACCCGTG CCCCCCGGCC 
     3721 TCGATTTCAA CCTCACGGAA TCAGACACTG TTAAGCGGAG GCCCAAGTGC CGGGAGAGAG 
     3781 AGCCACTGCA GACCGCACTG CTGGCCTTCG GAGTGGCCAG TGCCACGCCT GGCCCCGCTG 
     3841 CCCCACTGCC TTCCCCAACT CCTGGCGAGT CTCCTCCAGC TTCTAGCCTT CCCCAGCCCG 
     3901 AGCCCAGCAG CCTTCCAGCC CAAGGAGTTC CAACCCCCCT TGCTCCCAGC CCCGCCATGC 
     3961 AGCCTCCAGT GCCGCCCTGC CCAGGGCCAG GTCTGGAAAG CTCAGCAGCT AGTCGGTGGA 
     4021 ATGGGGAGAC AGAACCCCCG GCCGCCCCTG CTGCCCTCCT CAAGGTGCCC GGAGCAGGAA 
     4081 CAGCCCCCAA GCCTGTGTCG GTGGCCTGCA CCCAGCTGGC ATTTTCTGGC CCTAAGCTAG 
     4141 CGCCCCGGCT CGGCCCCCGC CCAGTGCCTC CTCCACGGCC TGAGAGCACT GGGACTGTGG 
     4201 GCCCAGGCCA GGCCCAGCAG AGACTGGAGC AGACCAGCTC GTCCCTGGCA GCTGCACTGA 
     4261 GAGCCGCAGA GAAGAGCATT GGCACCAAGG AGCAAGAGGG CACCCCCAGC GCCTCCACCA 
     4321 AGCACATTCT GGATGACATC AGCACCATGT TCGACGCCCT GGCTGACCAG CTGGACGCCA 
     4381 TGCTGGACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4441 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4501 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4561 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4621 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4681 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4741 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4801 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4861 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4921 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4981 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5041 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5101 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5161 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5221 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5281 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5341 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5401 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5461 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5521 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5581 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5641 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5701 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5761 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5821 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5881 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5941 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6001 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6061 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6121 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6181 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6241 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6301 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6361 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6421 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6481 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6541 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6601 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6661 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6721 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6781 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6841 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6901 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6961 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7021 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7081 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7141 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7201 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7261 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7321 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7381 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7441 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7501 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7561 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7681 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7741 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7801 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7861 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7921 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7981 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8041 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8101 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8161 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8221 CAAAAATTTA ACGCGAATTT T
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