LOCUS       RC227927                7380 bp    DNA     circular  SYN 
DEFINITION  PROM1 (Myc-DDK-tagged)-Human prominin 1 (PROM1), transcript variant 5
ACCESSION   NM_001145851
VERSION     NM_001145851.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3537..3628 
                     /label="Myc-DDK"  
     misc_feature    3658..4243 
                     /label="PolyA signal"  
     misc_feature    3715..3734 
                     /label="Restriction Enzyme"  
     misc_feature    4519..5191 
                     /label="ColE1"  
     misc_feature    5621..6415 
                     /label="Kan/Neo"  
     misc_feature    6450..6752 
                     /label="SV40 ori"  
     misc_feature    6838..7315 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3519..3525 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3519 
                     /label="PROM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTCGTA CTCGGCTCCC TGTTGCTGCT GGGGCTGTGC GGGAACTCCT 
     1081 TTTCAGGAGG GCAGCCTTCA TCCACAGATG CTCCTAAGGC TTGGAATTAT GAATTGCCTG 
     1141 CAACAAATTA TGAGACCCAA GACTCCCATA AAGCTGGACC CATTGGCATT CTCTTTGAAC 
     1201 TAGTGCATAT CTTTCTCTAT GTGGTACAGC CGCGTGATTT CCCAGAAGAT ACTTTGAGAA 
     1261 AATTCTTACA GAAGGCATAT GAATCCAAAA TTGATTATGA CAAGATTGTC TACTATGAAG 
     1321 CAGGGATTAT TCTATGCTGT GTCCTGGGGC TGCTGTTTAT TATTCTGATG CCTCTGGTGG 
     1381 GGTATTTCTT TTGTATGTGT CGTTGCTGTA ACAAATGTGG TGGAGAAATG CACCAGCGAC 
     1441 AGAAGGAAAA TGGGCCCTTC CTGAGGAAAT GCTTTGCAAT CTCCCTGTTG GTGATTTGTA 
     1501 TAATAATAAG CATTGGCATC TTCTATGGTT TTGTGGCAAA TCACCAGGTA AGAACCCGGA 
     1561 TCAAAAGGAG TCGGAAACTG GCAGATAGCA ATTTCAAGGA CTTGCGAACT CTCTTGAATG 
     1621 AAACTCCAGA GCAAATCAAA TATATATTGG CCCAGTACAA CACTACCAAG GACAAGGCGT 
     1681 TCACAGATCT GAACAGTATC AATTCAGTGC TAGGAGGCGG AATTCTTGAC CGACTGAGAC 
     1741 CCAACATCAT CCCTGTTCTT GATGAGATTA AGTCCATGGC AACAGCGATC AAGGAGACCA 
     1801 AAGAGGCGTT GGAGAACATG AACAGCACCT TGAAGAGCTT GCACCAACAA AGTACACAGC 
     1861 TTAGCAGCAG TCTGACCAGC GTGAAAACTA GCCTGCGGTC ATCTCTCAAT GACCCTCTGT 
     1921 GCTTGGTGCA TCCATCAAGT GAAACCTGCA ACAGCATCAG ATTGTCTCTA AGCCAGCTGA 
     1981 ATAGCAACCC TGAACTGAGG CAGCTTCCAC CCGTGGATGC AGAACTTGAC AACGTTAATA 
     2041 ACGTTCTTAG GACAGATTTG GATGGCCTGG TCCAACAGGG CTATCAATCC CTTAATGATA 
     2101 TACCTGACAG AGTACAACGC CAAACCACGA CTGTCGTAGC AGGTATCAAA AGGGTCTTGA 
     2161 ATTCCATTGG TTCAGATATC GACAATGTAA CTCAGCGTCT TCCTATTCAG GATATACTCT 
     2221 CAGCATTCTC TGTTTATGTT AATAACACTG AAAGTTACAT CCACAGAAAT TTACCTACAT 
     2281 TGGAAGAGTA TGATTCATAC TGGTGGCTGG GTGGCCTGGT CATCTGCTCT CTGCTGACCC 
     2341 TCATCGTGAT TTTTTACTAC CTGGGCTTAC TGTGTGGCGT GTGCGGCTAT GACAGGCATG 
     2401 CCACCCCGAC CACCCGAGGC TGTGTCTCCA ACACCGGAGG CGTCTTCCTC ATGGTTGGAG 
     2461 TTGGATTAAG TTTCCTCTTT TGCTGGATAT TGATGATCAT TGTGGTTCTT ACCTTTGTCT 
     2521 TTGGTGCAAA TGTGGAAAAA CTGATCTGTG AACCTTACAC GAGCAAGGAA TTATTCCGGG 
     2581 TTTTGGATAC ACCCTACTTA CTAAATGAAG ACTGGGAATA CTATCTCTCT GGGAAGCTAT 
     2641 TTAATAAATC AAAAATGAAG CTCACTTTTG AACAAGTTTA CAGTGACTGC AAAAAAAATA 
     2701 GAGGCACTTA CGGCACTCTT CACCTGCAGA ACAGCTTCAA TATCAGTGAA CATCTCAACA 
     2761 TTAATGAGCA TACTGGAAGC ATAAGCAGTG AATTGGAAAG TCTGAAGGTA AATCTTAATA 
     2821 TCTTTCTGTT GGGTGCAGCA GGAAGAAAAA ACCTTCAGGA TTTTGCTGCT TGTGGAATAG 
     2881 ACAGAATGAA TTATGACAGC TACTTGGCTC AGACTGGTAA ATCCCCCGCA GGAGTGAATC 
     2941 TTTTATCATT TGCATATGAT CTAGAAGCAA AAGCAAACAG TTTGCCCCCA GGAAATTTGA 
     3001 GGAACTCCCT GAAAAGAGAT GCACAAACTA TTAAAACAAT TCACCAGCAA CGAGTCCTTC 
     3061 CTATAGAACA ATCACTGAGC ACTCTATACC AAAGCGTCAA GATACTTCAA CGCACAGGGA 
     3121 ATGGATTGTT GGAGAGAGTA ACTAGGATTC TAGCTTCTCT GGATTTTGCT CAGAACTTCA 
     3181 TCACAAACAA TACTTCCTCT GTTATTATTG AGGAAACTAA GAAGTATGGG AGAACAATAA 
     3241 TAGGATATTT TGAACATTAT CTGCAGTGGA TCGAGTTCTC TATCAGTGAG AAAGTGGCAT 
     3301 CGTGCAAACC TGTGGCCACC GCTCTAGATA CTGCTGTTGA TGTCTTTCTG TGTAGCTACA 
     3361 TTATCGACCC CTTGAATTTG TTTTGGTTTG GCATAGGAAA AGCTACTGTA TTTTTACTTC 
     3421 CGGCTCTAAT TTTTGCGGTA AAACTGGCTA AGTACTATCG TCGAATGGAT TCGGAGGACG 
     3481 TGTACGATGA TGTTGAAACT ATACCCATGA AAAACCCATC ACAACATACG CGTACGCGGC 
     3541 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3601 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3661 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3721 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3781 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3841 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3901 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3961 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4021 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4081 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4141 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4201 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4261 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4321 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4381 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4441 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4501 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4561 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4621 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4681 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4741 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4801 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4861 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4921 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4981 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5041 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5101 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5161 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5221 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5281 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5341 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5401 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5461 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5521 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5581 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5641 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5701 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5761 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5821 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5881 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5941 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6001 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6061 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6121 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6181 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6241 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6301 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6361 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6421 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6481 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6541 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6601 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6661 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6721 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6781 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6841 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6901 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6961 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7021 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7081 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7141 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7201 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7261 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7321 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//