LOCUS       RC227911                7383 bp    DNA     circular  SYN 
DEFINITION  PROM1 (Myc-DDK-tagged)-Human prominin 1 (PROM1), transcript variant 6
ACCESSION   NM_001145850
VERSION     NM_001145850.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3540..3631 
                     /label="Myc-DDK"  
     misc_feature    3661..4246 
                     /label="PolyA signal"  
     misc_feature    3718..3737 
                     /label="Restriction Enzyme"  
     misc_feature    4522..5194 
                     /label="ColE1"  
     misc_feature    5624..6418 
                     /label="Kan/Neo"  
     misc_feature    6453..6755 
                     /label="SV40 ori"  
     misc_feature    6841..7318 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3522..3528 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3522 
                     /label="PROM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTCGTA CTCGGCTCCC TGTTGCTGCT GGGGCTGTGC GGGAACTCCT 
     1081 TTTCAGGAGG GCAGCCTTCA TCCACAGATG CTCCTAAGGC TTGGAATTAT GAATTGCCTG 
     1141 CAACAAATTA TGAGACCCAA GACTCCCATA AAGCTGGACC CATTGGCATT CTCTTTGAAC 
     1201 TAGTGCATAT CTTTCTCTAT GTGGTACAGC CGCGTGATTT CCCAGAAGAT ACTTTGAGAA 
     1261 AATTCTTACA GAAGGCATAT GAATCCAAAA TTGATTATGA CAAGCCAGAA ACTGTAATCT 
     1321 TAGGTCTAAA GATTGTCTAC TATGAAGCAG GGATTATTCT ATGCTGTGTC CTGGGGCTGC 
     1381 TGTTTATTAT TCTGATGCCT CTGGTGGGGT ATTTCTTTTG TATGTGTCGT TGCTGTAACA 
     1441 AATGTGGTGG AGAAATGCAC CAGCGACAGA AGGAAAATGG GCCCTTCCTG AGGAAATGCT 
     1501 TTGCAATCTC CCTGTTGGTG ATTTGTATAA TAATAAGCAT TGGCATCTTC TATGGTTTTG 
     1561 TGGCAAATCA CCAGGTAAGA ACCCGGATCA AAAGGAGTCG GAAACTGGCA GATAGCAATT 
     1621 TCAAGGACTT GCGAACTCTC TTGAATGAAA CTCCAGAGCA AATCAAATAT ATATTGGCCC 
     1681 AGTACAACAC TACCAAGGAC AAGGCGTTCA CAGATCTGAA CAGTATCAAT TCAGTGCTAG 
     1741 GAGGCGGAAT TCTTGACCGA CTGAGACCCA ACATCATCCC TGTTCTTGAT GAGATTAAGT 
     1801 CCATGGCAAC AGCGATCAAG GAGACCAAAG AGGCGTTGGA GAACATGAAC AGCACCTTGA 
     1861 AGAGCTTGCA CCAACAAAGT ACACAGCTTA GCAGCAGTCT GACCAGCGTG AAAACTAGCC 
     1921 TGCGGTCATC TCTCAATGAC CCTCTGTGCT TGGTGCATCC ATCAAGTGAA ACCTGCAACA 
     1981 GCATCAGATT GTCTCTAAGC CAGCTGAATA GCAACCCTGA ACTGAGGCAG CTTCCACCCG 
     2041 TGGATGCAGA ACTTGACAAC GTTAATAACG TTCTTAGGAC AGATTTGGAT GGCCTGGTCC 
     2101 AACAGGGCTA TCAATCCCTT AATGATATAC CTGACAGAGT ACAACGCCAA ACCACGACTG 
     2161 TCGTAGCAGG TATCAAAAGG GTCTTGAATT CCATTGGTTC AGATATCGAC AATGTAACTC 
     2221 AGCGTCTTCC TATTCAGGAT ATACTCTCAG CATTCTCTGT TTATGTTAAT AACACTGAAA 
     2281 GTTACATCCA CAGAAATTTA CCTACATTGG AAGAGTATGA TTCATACTGG TGGCTGGGTG 
     2341 GCCTGGTCAT CTGCTCTCTG CTGACCCTCA TCGTGATTTT TTACTACCTG GGCTTACTGT 
     2401 GTGGCGTGTG CGGCTATGAC AGGCATGCCA CCCCGACCAC CCGAGGCTGT GTCTCCAACA 
     2461 CCGGAGGCGT CTTCCTCATG GTTGGAGTTG GATTAAGTTT CCTCTTTTGC TGGATATTGA 
     2521 TGATCATTGT GGTTCTTACC TTTGTCTTTG GTGCAAATGT GGAAAAACTG ATCTGTGAAC 
     2581 CTTACACGAG CAAGGAATTA TTCCGGGTTT TGGATACACC CTACTTACTA AATGAAGACT 
     2641 GGGAATACTA TCTCTCTGGG AAGCTATTTA ATAAATCAAA AATGAAGCTC ACTTTTGAAC 
     2701 AAGTTTACAG TGACTGCAAA AAAAATAGAG GCACTTACGG CACTCTTCAC CTGCAGAACA 
     2761 GCTTCAATAT CAGTGAACAT CTCAACATTA ATGAGCATAC TGGAAGCATA AGCAGTGAAT 
     2821 TGGAAAGTCT GAAGGTAAAT CTTAATATCT TTCTGTTGGG TGCAGCAGGA AGAAAAAACC 
     2881 TTCAGGATTT TGCTGCTTGT GGAATAGACA GAATGAATTA TGACAGCTAC TTGGCTCAGA 
     2941 CTGGTAAATC CCCCGCAGGA GTGAATCTTT TATCATTTGC ATATGATCTA GAAGCAAAAG 
     3001 CAAACAGTTT GCCCCCAGGA AATTTGAGGA ACTCCCTGAA AAGAGATGCA CAAACTATTA 
     3061 AAACAATTCA CCAGCAACGA GTCCTTCCTA TAGAACAATC ACTGAGCACT CTATACCAAA 
     3121 GCGTCAAGAT ACTTCAACGC ACAGGGAATG GATTGTTGGA GAGAGTAACT AGGATTCTAG 
     3181 CTTCTCTGGA TTTTGCTCAG AACTTCATCA CAAACAATAC TTCCTCTGTT ATTATTGAGG 
     3241 AAACTAAGAA GTATGGGAGA ACAATAATAG GATATTTTGA ACATTATCTG CAGTGGATCG 
     3301 AGTTCTCTAT CAGTGAGAAA GTGGCATCGT GCAAACCTGT GGCCACCGCT CTAGATACTG 
     3361 CTGTTGATGT CTTTCTGTGT AGCTACATTA TCGACCCCTT GAATTTGTTT TGGTTTGGCA 
     3421 TAGGAAAAGC TACTGTATTT TTACTTCCGG CTCTAATTTT TGCGGTAAAA CTGGCTAAGT 
     3481 ACTATCGTCG AATGGATTCG GAGGACGTGT ACGATGACCC ATCACAACAT ACGCGTACGC 
     3541 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3601 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3661 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3721 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3781 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3841 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3901 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3961 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4021 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4081 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4141 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4201 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4261 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4321 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4381 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4441 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4501 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4561 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4621 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4681 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4741 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4801 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4861 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4921 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4981 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5041 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5101 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5161 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5221 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5281 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5341 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5401 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5461 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5521 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5581 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5641 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5701 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5761 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5821 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5881 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5941 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6001 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6061 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6121 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6181 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6241 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6301 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6361 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6421 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6481 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6541 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6601 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6661 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6721 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6781 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6841 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6901 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6961 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7021 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7081 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7141 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7201 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7261 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7321 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7381 TTT
//