LOCUS       RC227900                8058 bp    DNA     circular  SYN 
DEFINITION  LRIG3 (Myc-DDK-tagged)-Human leucine-rich repeats and immunoglobulin-like domains 3 (LRIG3), transcript variant 1
ACCESSION   NM_001136051
VERSION     NM_001136051.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4215..4306 
                     /label="Myc-DDK"  
     misc_feature    4336..4921 
                     /label="PolyA signal"  
     misc_feature    4393..4412 
                     /label="Restriction Enzyme"  
     misc_feature    5197..5869 
                     /label="ColE1"  
     misc_feature    6299..7093 
                     /label="Kan/Neo"  
     misc_feature    7128..7430 
                     /label="SV40 ori"  
     misc_feature    7516..7993 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4197..4203 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4197 
                     /label="LRIG3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGACGTA CTTCTGTTGT TCTCCCTCTG CTTGCTTTTT CACATTAGCA 
     1081 GACCGGACTT AAGTCACAAC AGATTATCTT TCATCAAGGC AAGTTCCATG AGCCACCTTC 
     1141 AAAGCCTTCG AGAAGTGAAA CTGAACAACA ATGAATTGGA GACCATTCCA AATCTGGGAC 
     1201 CAGTCTCGGC AAATATTACA CTTCTCTCCT TGGCTGGAAA CAGGATTGTT GAAATACTCC 
     1261 CTGAACATCT GAAAGAGTTT CAGTCCCTTG AAACTTTGGA CCTTAGCAGC AACAATATTT 
     1321 CAGAGCTCCA AACTGCATTT CCAGCCCTAC AGCTCAAATA TCTGTATCTC AACAGCAACC 
     1381 GAGTCACATC AATGGAACCT GGGTATTTTG ACAATTTGGC CAACACACTC CTTGTGTTAA 
     1441 AGCTGAACAG GAACCGAATC TCAGCTATCC CACCCAAGAT GTTTAAACTG CCCCAACTGC 
     1501 AACATCTCGA ATTGAACCGA AACAAGATTA AAAATGTAGA TGGACTGACA TTCCAAGGCC 
     1561 TTGGTGCTCT GAAGTCTCTG AAAATGCAAA GAAATGGAGT AACGAAACTT ATGGATGGAG 
     1621 CTTTTTGGGG GCTGAGCAAC ATGGAAATTT TGCAGCTGGA CCATAACAAC CTAACAGAGA 
     1681 TTACCAAAGG CTGGCTTTAC GGCTTGCTGA TGCTGCAGGA ACTTCATCTC AGCCAAAATG 
     1741 CCATCAACAG GATCAGCCCT GATGCCTGGG AGTTCTGCCA GAAGCTCAGT GAGCTGGACC 
     1801 TAACTTTCAA TCACTTATCA AGGTTAGATG ATTCAAGCTT CCTTGGCCTA AGCTTACTAA 
     1861 ATACACTGCA CATTGGGAAC AACAGAGTCA GCTACATTGC TGATTGTGCC TTCCGGGGGC 
     1921 TTTCCAGTTT AAAGACTTTG GATCTGAAGA ACAATGAAAT TTCCTGGACT ATTGAAGACA 
     1981 TGAATGGTGC TTTCTCTGGG CTTGACAAAC TGAGGCGACT GATACTCCAA GGAAATCGGA 
     2041 TCCGTTCTAT TACTAAAAAA GCCTTCACTG GTTTGGATGC ATTGGAGCAT CTAGACCTGA 
     2101 GTGACAACGC AATCATGTCT TTACAAGGCA ATGCATTTTC ACAAATGAAG AAACTGCAAC 
     2161 AATTGCATTT AAATACATCA AGCCTTTTGT GCGATTGCCA GCTAAAATGG CTCCCACAGT 
     2221 GGGTGGCGGA AAACAACTTT CAGAGCTTTG TAAATGCCAG TTGTGCCCAT CCTCAGCTGC 
     2281 TAAAAGGAAG AAGCATTTTT GCTGTTAGCC CAGATGGCTT TGTGTGTGAT GATTTTCCCA 
     2341 AACCCCAGAT CACGGTTCAG CCAGAAACAC AGTCGGCAAT AAAAGGTTCC AATTTGAGTT 
     2401 TCATCTGCTC AGCTGCCAGC AGCAGTGATT CCCCAATGAC TTTTGCTTGG AAAAAAGACA 
     2461 ATGAACTACT GCATGATGCT GAAATGGAAA ATTATGCACA CCTCCGGGCC CAAGGTGGCG 
     2521 AGGTGATGGA GTATACCACC ATCCTTCGGC TGCGCGAGGT GGAATTTGCC AGTGAGGGGA 
     2581 AATATCAGTG TGTCATCTCC AATCACTTTG GTTCATCCTA CTCTGTCAAA GCCAAGCTTA 
     2641 CAGTAAATAT GCTTCCCTCA TTCACCAAGA CCCCCATGGA TCTCACCATC CGAGCTGGGG 
     2701 CCATGGCACG CTTGGAGTGT GCTGCTGTGG GGCACCCAGC CCCCCAGATA GCCTGGCAGA 
     2761 AGGATGGGGG CACAGACTTC CCAGCTGCAC GGGAGAGACG CATGCATGTG ATGCCCGAGG 
     2821 ATGACGTGTT CTTTATCGTG GATGTGAAGA TAGAGGACAT TGGGGTATAC AGCTGCACAG 
     2881 CTCAGAACAG TGCAGGAAGT ATTTCAGCAA ATGCAACTCT GACTGTCCTA GAAACACCAT 
     2941 CATTTTTGCG GCCACTGTTG GACCGAACTG TAACCAAGGG AGAAACAGCC GTCCTACAGT 
     3001 GCATTGCTGG AGGAAGCCCT CCCCCTAAAC TGAACTGGAC CAAAGATGAT AGCCCATTGG 
     3061 TGGTAACCGA GAGGCACTTT TTTGCAGCAG GCAATCAGCT TCTGATTATT GTGGACTCAG 
     3121 ATGTCAGTGA TGCTGGGAAA TACACATGTG AGATGTCTAA CACCCTTGGC ACTGAGAGAG 
     3181 GAAACGTGCG CCTCAGTGTG ATCCCCACTC CAACCTGCGA CTCCCCTCAG ATGACAGCCC 
     3241 CATCGTTAGA CGATGACGGA TGGGCCACTG TGGGTGTCGT GATCATAGCC GTGGTTTGCT 
     3301 GTGTGGTGGG CACGTCACTC GTGTGGGTGG TCATCATATA CCACACAAGG CGGAGGAATG 
     3361 AAGATTGCAG CATTACCAAC ACAGATGAGA CCAACTTGCC AGCAGATATT CCTAGTTATT 
     3421 TGTCATCTCA GGGAACGTTA GCTGACAGGC AGGATGGGTA CGTGTCTTCA GAAAGTGGAA 
     3481 GCCACCACCA GTTTGTCACA TCTTCAGGTG CTGGATTTTT CTTACCACAA CATGACAGTA 
     3541 GTGGGACCTG CCATATTGAC AATAGCAGTG AAGCTGATGT GGAAGCTGCC ACAGATCTGT 
     3601 TCCTTTGTCC GTTTTTGGGA TCCACAGGCC CTATGTATTT GAAGGGAAAT GTGTATGGCT 
     3661 CAGATCCTTT TGAAACATAT CATACAGGTT GCAGTCCTGA CCCAAGAACA GTTTTAATGG 
     3721 ACCACTATGA GCCCAGTTAC ATAAAGAAAA AGGAGTGCTA CCCATGTTCT CATCCTTCAG 
     3781 AAGAATCCTG CGAACGGAGC TTCAGTAATA TATCGTGGCC TTCACATGTG AGGAAGCTAC 
     3841 TTAACACTAG TTACTCTCAC AATGAAGGAC CTGGAATGAA AAATCTGTGT CTAAACAAGT 
     3901 CCTCTTTAGA TTTTAGTGCA AATCCAGAGC CAGCGTCGGT TGCCTCGAGT AATTCTTTCA 
     3961 TGGGTACCTT TGGAAAAGCT CTCAGGAGAC CTCACCTAGA TGCCTATTCA AGCTTTGGAC 
     4021 AGCCATCAGA TTGTCAGCCA AGAGCCTTTT ATTTGAAAGC TCATTCTTCC CCAGACTTGG 
     4081 ACTCTGGGTC AGAGGAAGAT GGGAAAGAAA GGACAGATTT TCAGGAAGAA AATCACATTT 
     4141 GTACCTTTAA ACAGACTTTA GAAAACTACA GGACTCCAAA TTTTCAGTCT TATGACTTGG 
     4201 ACACAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4261 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4321 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4381 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4441 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4501 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4561 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4621 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4681 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4741 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4801 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4861 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4921 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4981 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5041 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5101 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5161 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5221 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5281 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5341 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5401 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5461 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5521 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5581 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5641 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5701 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5761 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5821 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5881 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5941 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6001 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6061 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6121 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6181 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6241 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6301 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6361 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6421 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6481 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6541 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6601 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6661 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6721 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6781 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6841 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6901 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6961 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7021 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7081 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7141 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7201 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7261 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7321 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7381 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7441 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7501 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7561 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7621 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7681 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7741 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7801 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7861 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7921 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7981 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8041 AAATTTAACG CGAATTTT
//