LOCUS       RC227773L4                9356 bp    DNA     circular  SYN 
DEFINITION  WRAP53 (Myc-DDK-tagged)-Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 3
ACCESSION   NM_001143991
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7647..7827 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8555..9201 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6522..6542 
                     /label="LR50"  
     misc_feature    4985..5713 
                     /label="mGFP"  
     misc_feature    5720..5785 
                     /label="P2A"  
     misc_feature    7875..8494 
                     /label="pBR322 Ori"  
     misc_feature    5786..6385 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4964..4970 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4964 
                     /label="WRAP53"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAAGACTTTG GAGACTCAAC CGTTAGCTCC 
     3361 GGACTGCTGT CCTTCAGACC AGGACCCAGC TCCAGCCCAT CCTTCTCCCC ACGCTTCCCC 
     3421 GATGAATAAA AATGCGGACT CTGAACTGAT GCCACCGCCT CCCGAAAGGG GGGATCCGCC 
     3481 CCGGTTGTCC CCAGATCCTG TGGCTGGCTC AGCTGTGTCC CAGGAGCTAC GGGAGGGGGA 
     3541 CCCAGTTTCT CTCTCCACTC CCCTGGAAAC AGAGTTTGGT TCCCCTAGTG AGTTGAGTCC 
     3601 TCGAATCGAG GAGCAAGAAC TTTCTGAAAA TACAAGCCTT CCTGCAGAAG AAGCAAACGG 
     3661 GAGCCTTTCT GAAGAAGAAG CGAACGGGCC AGAGTTGGGG TCTGGAAAAG CCATGGAAGA 
     3721 TACCTCTGGG GAACCCGCTG CAGAGGACGA GGGAGACACC GCTTGGAACT ACAGCTTCTC 
     3781 CCAGCTGCCT CGATTTCTCA GTGGTTCCTG GTCAGAGTTC AGCACCCAAC CTGAGAACTT 
     3841 CTTGAAAGGC TGTAAGTGGG CTCCTGACGG TTCCTGCATC TTGACCAATA GTGCTGATAA 
     3901 CATCTTGCGA ATTTATAACC TGCCCCCAGA GCTGTACCAT GAGGGGGAGC AGGTGGAATA 
     3961 TGCAGAAATG GTCCCTGTCC TTCGAATGGT GGAAGGTGAT ACCATCTATG ATTACTGCTG 
     4021 GTATTCTCTG ATGTCCTCAG CCCAGCCAGA CACCTCCTAC GTGGCCAGCA GCAGCCGGGA 
     4081 GAACCCGATT CATATCTGGG ACGCATTCAC TGGAGAGCTC CGGGCTTCCT TTCGCGCCTA 
     4141 CAACCACCTG GATGAGCTGA CGGCAGCCCA TTCGCTCTGC TTCTCCCCGG ATGGCTCCCA 
     4201 GCTCTTCTGT GGCTTCAACC GGACTGTGCG TGTTTTTTCC ACGGCCCGGC CTGGCCGAGA 
     4261 CTGCGAGGTC CGAGCCACAT TTGCAAAAAA GCAGGGCCAG AGCGGCATCA TCTCCTGCAT 
     4321 AGCCTTCAGC CCAGCCCAGC CCCTCTATGC CTGTGGCTCC TACGGCCGCT CCCTGGGTCT 
     4381 GTATGCCTGG GATGATGGCT CCCCTCTCGC CTTGCTGGGA GGGCACCAAG GGGGCATCAC 
     4441 CCACCTCTGC TTTCATCCCG ATGGCAACCG CTTCTTCTCA GGAGCCCGCA AGGATGCTGA 
     4501 GCTCCTGTGC TGGGATCTCC GGCAGTCTGG TTACCCACTG TGGTCCCTGG GTCGAGAGGT 
     4561 GACCACCAAT CAGCGCATCT ACTTCGATCT GGACCCGACC GGGCAGTTCC TAGTGAGTGG 
     4621 CAGCACGAGC GGGGCTGTCT CTGTGTGGGA CACGGACGGG CCTGGCAATG ATGGGAAGCC 
     4681 GGAGCCCGTG TTGAGTTTTC TGCCCCAGAA GGACTGCACC AATGGCGTGA GCCTGCACCC 
     4741 TAGCCTGCCT CTCCTGGCCA CTGCCTCCGG TCAGCGTGTG TTTCCTGAGC CCACAGAGAG 
     4801 TGGGGACGAA GGAGAGGAGC TGGGCCTTCC CTTGCTCTCC ACGCGCCACG TCCACCTTGA 
     4861 ATGTCGGCTT CAGCTCTGGT GGTGTGGGGG GGCGCCAGAC TCCAGCATCC CTGATGATCA 
     4921 CCAGGGCGAG AAAGGGCAGG GAGGAACGGA GGGAGGTGTG GGTGAGCTGA TAACGCGTAC 
     4981 GCGGCCGCTC GAGATGAGCG GGGGCGAGGA GCTGTTCGCC GGCATCGTGC CCGTGCTGAT 
     5041 CGAGCTGGAC GGCGACGTGC ACGGCCACAA GTTCAGCGTG CGCGGCGAGG GCGAGGGCGA 
     5101 CGCCGACTAC GGCAAGCTGG AGATCAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC 
     5161 CTGGCCCACC CTGGTGACCA CCCTCTGCTA CGGCATCCAG TGCTTCGCCC GCTACCCCGA 
     5221 GCACATGAAG ATGAACGACT TCTTCAAGAG CGCCATGCCC GAGGGCTACA TCCAGGAGCG 
     5281 CACCATCCAG TTCCAGGACG ACGGCAAGTA CAAGACCCGC GGCGAGGTGA AGTTCGAGGG 
     5341 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCAAGGAC TTCAAGGAGG ACGGCAACAT 
     5401 CCTGGGCCAC AAGCTGGAGT ACAGCTTCAA CAGCCACAAC GTGTACATCC GCCCCGACAA 
     5461 GGCCAACAAC GGCCTGGAGG CTAACTTCAA GACCCGCCAC AACATCGAGG GCGGCGGCGT 
     5521 GCAGCTGGCC GACCACTACC AGACCAACGT GCCCCTGGGC GACGGCCCCG TGCTGATCCC 
     5581 CATCAACCAC TACCTGAGCA CTCAGACCAA GATCAGCAAG GACCGCAACG AGGCCCGCGA 
     5641 CCACATGGTG CTCCTGGAGT CCTTCAGCGC CTGCTGCCAC ACCCACGGCA TGGACGAGCT 
     5701 GTACAGGTCC GGACTCAGAG TTTGGGTAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA 
     5761 GCAGGCTGGA GACGTGGAGG AGAACCCTGG ACCTATGACC GAGTACAAGC CCACGGTGCG 
     5821 CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA 
     5881 CTACCCCGCC ACGCGCCACA CCGTCGATCC GGATCGCCAC ATCGAGCGGG TCACCGAGCT 
     5941 GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA 
     6001 CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC 
     6061 CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT 
     6121 GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG 
     6181 CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA 
     6241 GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC 
     6301 CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG 
     6361 CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGATGTACA CCCAAACGGC CGGCCGCGGT 
     6421 CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG 
     6481 TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA 
     6541 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 
     6601 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 
     6661 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 
     6721 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 
     6781 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 
     6841 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 
     6901 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG 
     6961 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 
     7021 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 
     7081 GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC 
     7141 GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC 
     7201 AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA 
     7261 CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA 
     7321 AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT 
     7381 CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA 
     7441 GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG 
     7501 AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT 
     7561 GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT 
     7621 CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC 
     7681 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG 
     7741 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC 
     7801 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA 
     7861 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 
     7921 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 
     7981 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 
     8041 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 
     8101 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 
     8161 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 
     8221 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 
     8281 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA 
     8341 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 
     8401 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 
     8461 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 
     8521 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC 
     8581 ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA 
     8641 CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT 
     8701 TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC 
     8761 TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG 
     8821 GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT 
     8881 GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA 
     8941 AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA 
     9001 TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA 
     9061 ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT 
     9121 GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT 
     9181 GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT 
     9241 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     9301 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//