LOCUS       RC227761                9534 bp    DNA     circular  SYN 
DEFINITION  ROBO1 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 4
ACCESSION   NM_001145845
VERSION     NM_001145845.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5691..5782 
                     /label="Myc-DDK"  
     misc_feature    5812..6397 
                     /label="PolyA signal"  
     misc_feature    5869..5888 
                     /label="Restriction Enzyme"  
     misc_feature    6673..7345 
                     /label="ColE1"  
     misc_feature    7775..8569 
                     /label="Kan/Neo"  
     misc_feature    8604..8906 
                     /label="SV40 ori"  
     misc_feature    8992..9469 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5673..5679 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5673 
                     /label="ROBO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTGCGGAG CCCGCTCACT TTTACCTGTT TGGATTAATA TGTCTCTGTT 
     1081 CAGGCTCCCG TCTTCGTCAG GAAGATTTTC CACCTCGCAT TGTTGAACAC CCTTCAGACC 
     1141 TGATTGTCTC AAAAGGAGAA CCTGCAACTT TGAACTGCAA AGCTGAAGGC CGCCCCACAC 
     1201 CCACTATTGA ATGGTACAAA GGGGGAGAGA GAGTGGAGAC AGACAAAGAT GACCCTCGCT 
     1261 CACACCGAAT GTTGCTGCCG AGTGGATCTT TATTTTTCTT ACGTATAGTA CATGGACGGA 
     1321 AAAGTAGACC TGATGAAGGA GTCTATGTCT GTGTAGCAAG GAATTACCTT GGAGAGGCTG 
     1381 TGAGCCACAA TGCATCGCTG GAAGTAGCCA TACTTCGGGA TGACTTCAGA CAAAACCCTT 
     1441 CGGATGTCAT GGTTGTAGTA GGAGAGCCTG CAGTAATGGA ATGCCAACCT CCACGAGGCC 
     1501 ATCCTGAGCC CACCATTTCA TGGAAGAAAG ATGGCTCTCC ACTGGATGAT AAAGATGAAA 
     1561 GAATAACTAT ACGAGGAGGA AAGCTCATGA TCACTTACAC CCGTAAAAGT GACGCTGGCA 
     1621 AATATGTTTG TGTTGGTACC AATATGGTTG GGGAACGTGA GAGTGAAGTA GCCGAGCTGA 
     1681 CTGTCTTAGA GAGACCATCA TTTGTGAAGA GACCCAGTAA CTTGGCAGTA ACTGTGGATG 
     1741 ACAGTGCAGA ATTTAAATGT GAGGCCCGAG GTGACCCTGT ACCTACAGTA CGATGGAGGA 
     1801 AAGATGATGG AGAGCTGCCC AAATCCAGAT ATGAAATCCG AGATGATCAT ACCTTGAAAA 
     1861 TTAGGAAGGT GACAGCTGGT GACATGGGTT CATACACTTG TGTTGCAGAA AATATGGTGG 
     1921 GCAAAGCTGA AGCATCTGCT ACTCTGACTG TTCAAGTTGG GTCTGAACCT CCACATTTTG 
     1981 TTGTGAAACC CCGTGACCAG GTTGTTGCTT TGGGACGGAC TGTAACTTTT CAGTGTGAAG 
     2041 CAACCGGAAA TCCTCAACCA GCTATTTTCT GGAGGAGAGA AGGGAGTCAG AATCTACTTT 
     2101 TCTCATATCA ACCACCACAG TCATCCAGCC GATTTTCAGT CTCCCAGACT GGCGACCTCA 
     2161 CAATTACTAA TGTCCAGCGA TCTGATGTTG GTTATTACAT CTGCCAGACT TTAAATGTTG 
     2221 CTGGAAGCAT CATCACAAAG GCATATTTGG AAGTTACAGA TGTGATTGCA GATCGGCCTC 
     2281 CCCCAGTTAT TCGACAAGGT CCTGTGAATC AGACTGTAGC CGTGGATGGC ACTTTCGTCC 
     2341 TCAGCTGTGT GGCCACAGGC AGTCCAGTGC CCACCATTCT GTGGAGAAAG GATGGAGTCC 
     2401 TCGTTTCAAC CCAAGACTCT CGAATCAAAC AGTTGGAGAA TGGAGTACTG CAGATCCGAT 
     2461 ATGCTAAGCT GGGTGATACT GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAAG 
     2521 CAACATGGAG TGCTTACATT GAAGTTCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC 
     2581 CTACTGACCC AAATTTAATC CCTAGTGCCC CATCAAAACC TGAAGTGACA GATGTCAGCA 
     2641 GAAATACAGT CACATTATCG TGGCAACCAA ATTTGAATTC AGGAGCAACT CCAACATCTT 
     2701 ATATTATAGA AGCCTTCAGC CATGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG 
     2761 TGAAAACAGA AACATCTGCC ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG 
     2821 TGAGGGCAGC TAATGCATAT GGAATTAGTG ATCCAAGCCA AATATCAGAT CCAGTGAAAA 
     2881 CACAAGATGT CCTACCAACA AGTCAGGGGG TGGACCACAA GCAGGTCCAG AGAGAGCTGG 
     2941 GAAATGCTGT TCTGCACCTC CACAACCCCA CCGTCCTTTC TTCCTCTTCC ATCGAAGTGC 
     3001 ACTGGACAGT AGATCAACAG TCTCAGTATA TACAAGGATA TAAAATTCTC TATCGGCCAT 
     3061 CTGGAGCCAA CCACGGAGAA TCAGACTGGT TAGTTTTTGA AGTGAGGACG CCAGCCAAAA 
     3121 ACAGTGTGGT AATCCCTGAT CTCAGAAAGG GAGTCAACTA TGAAATTAAG GCTCGCCCTT 
     3181 TTTTTAATGA ATTTCAAGGA GCAGATAGTG AAATCAAGTT TGCCAAAACC CTGGAAGAAG 
     3241 CACCCAGTGC CCCACCCCAA GGTGTAACTG TATCCAAGAA TGATGGAAAC GGAACTGCAA 
     3301 TTCTAGTTAG TTGGCAGCCA CCTCCAGAAG ACACTCAAAA TGGAATGGTC CAAGAGTATA 
     3361 AGGTTTGGTG TCTGGGCAAT GAAACTCGAT ACCACATCAA CAAAACAGTG GATGGTTCCA 
     3421 CCCTTTCCGT GGTCATTCCC TTTCTTGTTC CTGGAATCCG ATACAGTGTG GAAGTGGCAG 
     3481 CCAGCACTGG GGCTGGGTCT GGGGTAAAGA GTGAGCCTCA GTTCATCCAG CTGGATGCCC 
     3541 ATGGAAACCC TGTGTCACCT GAGGACCAAG TCAGCCTCGC TCAGCAGATT TCAGATGTGG 
     3601 TGAAGCAGCC GGCCTTCATA GCAGGTATTG GAGCAGCCTG TTGGATCATC CTCATGGTCT 
     3661 TCAGCATCTG GCTTTATCGA CACCGCAAGA AGAGAAACGG ACTTACTAGT ACCTACGCGG 
     3721 GTATCAGAAA AGTAACTTAC CAGAGAGGAG GCGAAGCTGT CAGCAGTGGA GGGAGGCCTG 
     3781 GACTTCTCAA CATCAGTGAA CCTGCCGCGC AGCCATGGCT GGCAGACACG TGGCCTAATA 
     3841 CTGGCAACAA CCACAATGAC TGCTCCATCA GCTGCTGCAC GGCAGGCAAT GGAAACAGCG 
     3901 ACAGCAACCT CACTACCTAC AGTCGCCCAG GGCAGCCTAC TCCTTACGCC ACCACTCAGC 
     3961 TCATCCAGTC AAACCTCAGC AACAACATGA ACAATGGCAG CGGGGACTCT GGCGAGAAGC 
     4021 ACTGGAAACC ACTGGGACAG CAGAAACAAG AAGTGGCACC AGTTCAGTAC AACATCGTGG 
     4081 AGCAAAACAA GCTGAACAAA GATTATCGAG CAAATGACAC AGTTCCTCCA ACTATCCCAT 
     4141 ACAACCAATC ATACGACCAG AACACAGGAG GATCCTACAA CAGCTCAGAC CGGGGCAGTA 
     4201 GTACATCTGG GAGTCAGGGG CACAAGAAAG GGGCAAGAAC ACCCAAGGTA CCAAAACAGG 
     4261 GTGGCATGAA CTGGGCAGAC CTGCTTCCTC CTCCCCCAGC ACATCCTCCT CCACACAGCA 
     4321 ATAGCGAAGA GTACAACATT TCTGTAGATG AAAGCTATGA CCAAGAAATG CCATGTCCCG 
     4381 TGCCACCAGC AAGGATGTAT TTGCAACAAG ATGAATTAGA AGAGGAGGAA GATGAACGAG 
     4441 GCCCCACTCC CCCTGTTCGG GGAGCAGCCT CTTCTCCAGC TGCCGTGTCC TATAGCCATC 
     4501 AGTCCACTGC CACTCTGACT CCCTCCCCAC AGGAAGAACT CCAGCCCATG TTACAGGATT 
     4561 GTCCAGAGGA GACTGGCCAC ATGCAGCACC AGCCCGACAG GAGACGGCAG CCTGTGAGTC 
     4621 CTCCTCCACC ACCACGGCCG ATCTCCCCTC CACATACCTA TGGCTACATT TCAGGACCCC 
     4681 TGGTCTCAGA TATGGATACG GATGCGCCAG AAGAGGAAGA AGACGAAGCC GACATGGAGG 
     4741 TAGCCAAGAT GCAAACCAGA AGGCTTTTGT TACGTGGGCT TGAGCAGACA CCTGCCTCCA 
     4801 GTGTTGGGGA CCTGGAGAGC TCTGTCACGG GGTCCATGAT CAACGGCTGG GGCTCAGCCT 
     4861 CAGAGGAGGA CAACATTTCC AGCGGACGCT CCAGTGTTAG TTCTTCGGAC GGCTCCTTTT 
     4921 TCACTGATGC TGACTTTGCC CAGGCAGTCG CAGCAGCGGC AGAGTATGCT GGTCTGAAAG 
     4981 TAGCACGACG GCAAATGCAG GATGCTGCTG GCCGTCGACA TTTTCATGCG TCTCAGTGCC 
     5041 CTAGGCCCAC AAGTCCCGTG TCTACAGACA GCAACATGAG TGCCGCCGTA ATGCAGAAAA 
     5101 CCAGACCAGC CAAGAAACTG AAACACCAGC CAGGACATCT GCGCAGAGAA ACCTACACAG 
     5161 ATGATCTTCC ACCACCTCCT GTGCCGCCAC CTGCTATAAA GTCACCTACT GCCCAATCCA 
     5221 AGACACAGCT GGAAGTACGA CCTGTAGTGG TGCCAAAACT CCCTTCTATG GATGCAAGAA 
     5281 CAGACAGATC ATCAGACAGA AAAGGAAGCA GTTACAAGGG GAGAGAAGTG TTGGATGGAA 
     5341 GACAGGTTGT TGACATGCGA ACAAATCCAG GTGATCCCAG AGAAGCACAG GAACAGCAAA 
     5401 ATGACGGGAA AGGACGTGGA AACAAGGCAG CAAAACGAGA CCTTCCACCA GCAAAGACTC 
     5461 ATCTCATCCA AGAGGATATT CTACCTTATT GTAGACCTAC TTTTCCAACA TCAAATAATC 
     5521 CCAGAGATCC CAGTTCCTCA AGCTCAATGT CATCAAGAGG ATCAGGAAGC AGACAAAGAG 
     5581 AACAAGCAAA TGTAGGTCGA AGAAATATTG CAGAAATGCA GGTACTTGGA GGATATGAAA 
     5641 GAGGAGAAGA TAATAATGAA GAATTAGAGG AAACTGAAAG CACGCGTACG CGGCCGCTCG 
     5701 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5761 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5821 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5881 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5941 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     6001 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     6061 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     6121 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     6181 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     6241 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     6301 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     6361 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6421 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6481 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6541 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6601 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6661 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6721 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6781 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6841 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6901 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6961 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     7021 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     7081 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     7141 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     7201 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     7261 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     7321 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     7381 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7441 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7501 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7561 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7621 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7681 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7741 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7801 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7861 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7921 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7981 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     8041 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     8101 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     8161 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     8221 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     8281 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     8341 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     8401 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8461 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8521 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8581 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8641 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8701 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8761 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8821 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8881 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8941 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     9001 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     9061 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     9121 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     9181 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     9241 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     9301 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     9361 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9421 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9481 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//