LOCUS       RC227736                8517 bp    DNA     circular  SYN 
DEFINITION  GRM5 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 5 (GRM5), transcript variant a
ACCESSION   NM_001143831
VERSION     NM_001143831.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4674..4765 
                     /label="Myc-DDK"  
     misc_feature    4795..5380 
                     /label="PolyA signal"  
     misc_feature    4852..4871 
                     /label="Restriction Enzyme"  
     misc_feature    5656..6328 
                     /label="ColE1"  
     misc_feature    6758..7552 
                     /label="Kan/Neo"  
     misc_feature    7587..7889 
                     /label="SV40 ori"  
     misc_feature    7975..8452 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4656..4662 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4656 
                     /label="GRM5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCTTCTG TTGATCCTGT CAGTCTTACT TTTGAAAGAA GATGTCCGTG 
     1081 GGAGTGCACA GTCCAGTGAG AGGAGGGTGG TGGCTCACAT GCCGGGTGAC ATCATTATTG 
     1141 GAGCTCTCTT TTCTGTTCAT CACCAGCCTA CTGTGGACAA AGTTCATGAG AGGAAGTGTG 
     1201 GGGCGGTCCG TGAACAGTAT GGCATTCAGA GAGTGGAGGC CATGCTGCAT ACCCTGGAAA 
     1261 GGATCAATTC AGACCCCACA CTCTTGCCCA ACATCACACT GGGCTGTGAG ATAAGGGACT 
     1321 CCTGCTGGCA TTCGGCTGTG GCCCTAGAGC AGAGCATTGA GTTCATAAGA GATTCCCTCA 
     1381 TTTCTTCAGA AGAGGAAGAA GGCTTGGTAC GCTGTGTGGA TGGCTCCTCC TCTTCCTTCC 
     1441 GCTCCAAGAA GCCCATAGTA GGGGTCATTG GGCCTGGCTC CAGTTCTGTA GCCATTCAGG 
     1501 TCCAGAATTT GCTCCAGCTT TTCAACATAC CTCAGATTGC TTACTCAGCA ACCAGCATGG 
     1561 ATCTGAGTGA CAAGACTCTG TTCAAATATT TCATGAGGGT TGTGCCTTCA GATGCTCAGC 
     1621 AGGCAAGGGC CATGGTGGAC ATAGTGAAGA GGTACAACTG GACCTATGTA TCAGCCGTGC 
     1681 ACACAGAAGG CAACTATGGA GAAAGTGGGA TGGAAGCCTT CAAAGATATG TCAGCGAAGG 
     1741 AAGGGATTTG CATCGCCCAC TCTTACAAAA TCTACAGTAA TGCAGGGGAG CAGAGCTTTG 
     1801 ATAAGCTGCT GAAGAAGCTC ACAAGTCACT TGCCCAAGGC CCGGGTGGTG GCCTGCTTCT 
     1861 GTGAGGGCAT GACGGTGAGA GGTCTGCTGA TGGCCATGAG GCGCCTGGGT CTAGCGGGAG 
     1921 AATTTCTGCT TCTGGGCAGT GATGGCTGGG CTGACAGGTA TGATGTGACA GATGGATATC 
     1981 AGCGAGAAGC TGTTGGTGGC ATCACAATCA AGCTCCAATC TCCCGATGTC AAGTGGTTTG 
     2041 ATGATTATTA TCTGAAGCTC CGGCCAGAAA CAAACCACCG AAACCCTTGG TTTCAAGAAT 
     2101 TTTGGCAGCA TCGTTTTCAG TGCCGACTGG AAGGGTTTCC ACAGGAGAAC AGCAAATACA 
     2161 ACAAGACTTG CAATAGTTCT CTGACTCTGA AAACACATCA TGTTCAGGAT TCCAAAATGG 
     2221 GATTTGTGAT CAACGCCATC TATTCGATGG CCTATGGGCT CCACAACATG CAGATGTCCC 
     2281 TCTGCCCAGG CTATGCAGGA CTCTGTGATG CCATGAAGCC AATTGATGGA CGGAAACTTT 
     2341 TGGAGTCCCT GATGAAAACC AATTTTACTG GGGTTTCTGG AGATACGATC CTATTCGATG 
     2401 AGAATGGAGA CTCTCCAGGA AGGTATGAAA TAATGAATTT CAAGGAAATG GGAAAAGATT 
     2461 ACTTTGATTA TATCAACGTT GGAAGTTGGG ACAATGGAGA ATTAAAAATG GATGATGATG 
     2521 AAGTATGGTC CAAGAAAAGC AACATCATCA GATCTGTGTG CAGTGAACCA TGTGAGAAAG 
     2581 GCCAGATCAA GGTGATCCGA AAGGGAGAAG TCAGCTGTTG TTGGACCTGT ACACCTTGTA 
     2641 AGGAGAATGA GTATGTCTTT GATGAGTACA CATGCAAGGC ATGCCAACTG GGGTCTTGGC 
     2701 CCACTGATGA TCTCACAGGT TGTGACTTGA TCCCAGTACA GTATCTTCGA TGGGGTGACC 
     2761 CTGAACCCAT TGCAGCTGTG GTGTTTGCCT GCCTTGGCCT CCTGGCCACC CTGTTTGTTA 
     2821 CTGTAGTCTT CATCATTTAC CGTGATACAC CAGTAGTCAA GTCCTCAAGC AGGGAACTCT 
     2881 GCTACATTAT CCTTGCTGGC ATCTGCCTGG GCTACTTATG TACCTTCTGC CTCATTGCGA 
     2941 AGCCCAAACA GATTTACTGC TACCTTCAGA GAATTGGCAT TGGTCTCTCC CCAGCCATGA 
     3001 GCTACTCAGC CCTTGTAACA AAGACCAACC GTATTGCAAG GATCCTGGCT GGCAGCAAGA 
     3061 AGAAGATCTG TACCAAAAAG CCCAGATTCA TGAGTGCCTG TGCCCAGCTA GTGATTGCTT 
     3121 TCATTCTCAT ATGCATCCAG TTGGGCATCA TCGTTGCCCT CTTTATAATG GAGCCTCCTG 
     3181 ACATAATGCA TGACTACCCA AGCATTCGAG AAGTCTACCT GATCTGTAAC ACCACCAACC 
     3241 TAGGAGTTGT CACTCCACTT GGATACAATG GATTGTTGAT TTTGAGCTGC ACCTTCTATG 
     3301 CGTTCAAGAC CAGAAATGTT CCAGCTAACT TCAACGAGGC CAAGTATATC GCCTTCACAA 
     3361 TGTACACGAC CTGCATTATA TGGCTAGCTT TTGTGCCAAT CTACTTTGGC AGCAACTACA 
     3421 AAATCATCAC CATGTGTTTC TCGGTCAGCC TCAGTGCCAC AGTGGCCCTA GGCTGCATGT 
     3481 TTGTGCCGAA GGTGTACATC ATCCTGGCCA AACCAGAGAG AAACGTGCGC AGCGCCTTCA 
     3541 CCACATCTAC CGTGGTGCGC ATGCATGTAG GGGATGGCAA GTCATCCTCC GCAGCCAGCA 
     3601 GATCCAGCAG CCTAGTCAAC CTGTGGAAGA GAAGGGGCTC CTCTGGGGAA ACCTTAAGGT 
     3661 ACAAAGACAG GAGACTGGCC CAGCACAAGT CGGAAATAGA GTGTTTCACC CCCAAAGGGA 
     3721 GTATGGGGAA TGGTGGGAGA GCAACAATGA GCAGTTCCAA TGGAAAATCC GTCACGTGGG 
     3781 CCCAGAATGA GAAGAGCAGC CGGGGGCAGC ACCTGTGGCA GCGCCTGTCC ATCCACATCA 
     3841 ACAAGAAAGA AAACCCCAAC CAAACGGCCG TCATCAAGCC CTTCCCCAAG AGCACGGAGA 
     3901 GCCGTGGCCT GGGCGCTGGC GCTGGCGCAG GCGGGAGCGC TGGGGGCGTG GGGGCCACGG 
     3961 GCGGTGCGGG CTGCGCAGGC GCCGGCCCAG GCGGGCCCGA GTCCCCAGAC GCCGGCCCCA 
     4021 AGGCGCTGTA TGATGTGGCC GAGGCTGAGG AGCACTTCCC GGCGCCCGCG CGGCCGCGCT 
     4081 CACCGTCGCC CATCAGCACG CTGAGCCACC GCGCGGGCTC GGCCAGCCGC ACGGACGACG 
     4141 ATGTGCCGTC GCTGCACTCG GAGCCTGTGG CGCGCAGCAG CTCCTCGCAG GGCTCCCTCA 
     4201 TGGAGCAGAT CAGCAGTGTG GTCACCCGCT TCACGGCCAA CATCAGCGAG CTCAACTCCA 
     4261 TGATGCTGTC CACCGCGGCC CCCAGCCCCG GCGTCGGCGC CCCGCTCTGC TCGTCCTACC 
     4321 TGATCCCCAA AGAGATCCAG TTGCCCACGA CCATGACGAC CTTTGCCGAA ATCCAGCCTC 
     4381 TGCCGGCCAT CGAAGTCACG GGCGGCGCGC AGCCCGCGGC AGGGGCGCAG GCGGCTGGGG 
     4441 ACGCGGCCCG GGAGAGCCCC GCGGCCGGTC CCGAGGCTGC GGCCGCCAAG CCAGACCTGG 
     4501 AGGAGCTGGT GGCTCTCACC CCGCCGTCCC CCTTCAGAGA CTCGGTGGAC TCGGGGAGCA 
     4561 CAACCCCCAA CTCGCCAGTG TCCGAGTCGG CCCTCTGTAT CCCGTCGTCT CCCAAATATG 
     4621 ACACTCTTAT CATAAGAGAT TACACTCAGA GCTCCTCGTC GTTGACGCGT ACGCGGCCGC 
     4681 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4741 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4801 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4861 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4921 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4981 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5041 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5101 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5161 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5221 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5281 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5341 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5401 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5461 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5521 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5581 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5641 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5701 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5761 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5821 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5881 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5941 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6001 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6061 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6121 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6181 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6241 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6301 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6361 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6421 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6481 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6541 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6601 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6661 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6721 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6781 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6841 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6901 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6961 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7021 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7081 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7141 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7201 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7261 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7321 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7381 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7441 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7501 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7561 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7621 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7681 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7741 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7801 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7861 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7921 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7981 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8041 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8101 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8161 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8221 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8281 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8341 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8401 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8461 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//