LOCUS       RC227728L3                9410 bp    DNA     circular  SYN 
DEFINITION  SP1 (Myc-DDK-tagged)-Human Sp1 transcription factor (SP1), transcript variant 2
ACCESSION   NM_003109
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7701..7881 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8609..9268 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7929..8548 
                     /label="ColE1"  
     misc_feature    5741..5764 
                     /label="DDK"  
     misc_feature    6576..6596 
                     /label="LR50"  
     misc_feature    5693..5764 
                     /label="MYC"  
     misc_feature    5774..5839 
                     /label="P2A"  
     misc_feature    5840..6439 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6585..7176 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5675..5681 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5675 
                     /label="SP1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGCGACCAA GATCACTCCA TGGATGAAAT 
     3361 GACAGCTGTG GTGAAAATTG AAAAAGGAGT TGGTGGCAAT AATGGGGGCA ATGGTAATGG 
     3421 TGGTGGTGCC TTTTCACAGG CTCGAAGTAG CAGCACAGGC AGTAGCAGCA GCACTGGAGG 
     3481 AGGAGGGCAG GAGTCCCAGC CATCCCCTTT GGCTCTGCTG GCAGCAACTT GCAGCAGAAT 
     3541 TGAGTCACCC AATGAGAACA GCAACAACTC CCAGGGCCCG AGTCAGTCAG GGGGAACAGG 
     3601 TGAGCTTGAC CTCACAGCCA CACAACTTTC ACAGGGTGCC AATGGCTGGC AGATCATCTC 
     3661 TTCCTCCTCT GGGGCTACCC CTACCTCAAA GGAACAGAGT GGCAGCAGTA CCAATGGCAG 
     3721 CAATGGCAGT GAGTCTTCCA AGAATCGCAC AGTCTCTGGT GGGCAGTATG TTGTGGCTGC 
     3781 CGCTCCCAAC TTACAGAACC AGCAAGTTCT GACAGGACTA CCTGGAGTGA TGCCTAATAT 
     3841 TCAGTATCAA GTAATCCCAC AGTTCCAGAC CGTTGATGGG CAACAGCTGC AGTTTGCTGC 
     3901 CACTGGGGCC CAAGTGCAGC AGGATGGTTC TGGTCAAATA CAGATCATAC CAGGTGCAAA 
     3961 CCAACAGATT ATCACAAATC GAGGAAGTGG AGGCAACATC ATTGCTGCTA TGCCAAACCT 
     4021 ACTCCAGCAG GCTGTCCCCC TCCAAGGCCT GGCTAATAAT GTACTCTCAG GACAGACTCA 
     4081 GTATGTGACC AATGTACCAG TGGCCCTGAA TGGGAACATC ACCTTGCTAC CTGTCAACAG 
     4141 CGTTTCTGCA GCTACCTTGA CTCCCAGCTC TCAGGCAGTC ACGATCAGCA GCTCTGGGTC 
     4201 CCAGGAGAGT GGCTCACAGC CTGTCACCTC AGGGACTACC ATCAGTTCTG CCAGCTTGGT 
     4261 ATCATCACAA GCCAGTTCCA GCTCCTTTTT CACCAATGCC AATAGCTACT CAACTACTAC 
     4321 TACCACCAGC AACATGGGAA TTATGAACTT TACTACCAGT GGATCATCAG GGACCAACTC 
     4381 TCAAGGCCAG ACACCCCAGA GGGTCAGTGG GCTACAGGGG TCTGATGCTC TGAACATCCA 
     4441 GCAAAACCAG ACATCTGGAG GCTCATTGCA AGCAGGCCAG CAAAAAGAAG GAGAGCAAAA 
     4501 CCAGCAGACA CAGCAGCAAC AAATTCTTAT CCAGCCTCAG CTAGTTCAAG GGGGACAGGC 
     4561 CCTCCAGGCC CTCCAAGCAG CACCATTGTC AGGGCAGACC TTTACAACTC AAGCCATCTC 
     4621 CCAGGAAACC CTCCAGAACC TCCAGCTTCA GGCTGTTCCA AACTCTGGTC CCATCATCAT 
     4681 CCGGACACCA ACAGTGGGGC CCAATGGACA GGTCAGTTGG CAGACTCTAC AGCTGCAGAA 
     4741 CCTCCAAGTT CAGAACCCAC AAGCCCAAAC AATCACCTTA GCCCCAATGC AGGGTGTTTC 
     4801 CTTGGGGCAG ACCAGCAGCA GCAACACCAC TCTCACACCC ATTGCCTCAG CTGCTTCCAT 
     4861 TCCTGCTGGC ACAGTCACTG TGAATGCTGC TCAACTCTCC TCCATGCCAG GCCTCCAGAC 
     4921 CATTAACCTC AGTGCATTGG GTACTTCAGG AATCCAGGTG CACCCAATTC AAGGCCTGCC 
     4981 GTTGGCTATA GCAAATGCCC CAGGTGATCA TGGAGCTCAG CTTGGTCTCC ATGGGGCTGG 
     5041 TGGTGATGGA ATACATGATG ACACAGCAGG TGGAGAGGAA GGAGAAAACA GCCCAGATGC 
     5101 CCAACCCCAA GCCGGTCGGA GGACCCGGCG GGAAGCATGC ACCTGCCCCT ACTGTAAAGA 
     5161 CAGTGAAGGA AGGGGCTCGG GGGATCCTGG CAAAAAGAAA CAGCATATTT GCCACATCCA 
     5221 AGGCTGTGGG AAAGTGTATG GCAAGACCTC TCACCTGCGG GCACACTTGC GCTGGCATAC 
     5281 AGGCGAGAGG CCATTTATGT GTACCTGGTC ATACTGTGGG AAACGCTTCA CACGTTCGGA 
     5341 TGAGCTACAG AGGCACAAAC GTACACACAC AGGTGAGAAG AAATTTGCCT GCCCTGAGTG 
     5401 TCCTAAGCGC TTCATGAGGA GTGACCACCT GTCAAAACAT ATCAAGACCC ACCAGAATAA 
     5461 GAAGGGAGGC CCAGGTGTAG CTCTGAGTGT GGGCACTTTG CCCCTGGACA GTGGGGCAGG 
     5521 TTCAGAAGGC AGTGGCACTG CCACTCCTTC AGCCCTTATT ACCACCAATA TGGTAGCCAT 
     5581 GGAGGCCATC TGTCCAGAGG GCATTGCCCG TCTTGCCAAC AGTGGCATCA ACGTCATGCA 
     5641 GGTGGCAGAT CTGCAGTCCA TTAATATCAG TGGCAATGGC TTCACGCGTA CGCGGCCGCT 
     5701 CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA 
     5761 TGACGACGAT AAGGTTTGGG TAGGAAGCGG AGCTACTAAC TTCAGCCTGC TGAAGCAGGC 
     5821 TGGAGACGTG GAGGAGAACC CTGGACCTAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC 
     5881 CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC 
     5941 CGCCACGCGC CACACCGTCG ATCCGGATCG CCACATCGAG CGGGTCACCG AGCTGCAAGA 
     6001 ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC 
     6061 CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT 
     6121 CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG 
     6181 CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC 
     6241 GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC 
     6301 CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA 
     6361 CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG 
     6421 GTGCATGACC CGCAAGCCCG GTGCCTGATG TACACCCAAA CGGCCGGCCG CGGTCTGTAC 
     6481 AAGTAGGATT CGTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAC 
     6541 ATGTTTAAGG GTTCCGGTTC CACTAGGTAC AATTCGATAT CAAGCTTATC GATAATCAAC 
     6601 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 
     6661 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 
     6721 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 
     6781 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 
     6841 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 
     6901 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 
     6961 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG 
     7021 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 
     7081 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 
     7141 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGTCGACCTC 
     7201 GATCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC TACCAATGCT 
     7261 GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT CACACCTCAG 
     7321 GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA 
     7381 AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT TGATCTGTGG 
     7441 ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG GCCAGGGATC 
     7501 AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA AGAGAAGGTA 
     7561 GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT GCATGGGATG 
     7621 GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC 
     7681 ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC 
     7741 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA 
     7801 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA 
     7861 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGCATGTGAG CAAAAGGCCA GCAAAAGGCC 
     7921 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 
     7981 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 
     8041 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 
     8101 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 
     8161 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 
     8221 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 
     8281 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 
     8341 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA 
     8401 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 
     8461 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 
     8521 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 
     8581 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGATTA CGCCCCGCCC TGCCACTCAT 
     8641 CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA CAAACGGCAT 
     8701 GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA 
     8761 TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA 
     8821 AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT 
     8881 AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA 
     8941 AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG 
     9001 TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA 
     9061 ACTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT 
     9121 GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG GTCTGGTTAT 
     9181 AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA 
     9241 TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATACT CTTCCTTTTT CAATATTATT 
     9301 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     9361 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//