LOCUS       RC227617                7917 bp    DNA     circular  SYN 
DEFINITION  ITGAV (Myc-DDK-tagged)-Human integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) (ITGAV), transcript variant 3
ACCESSION   NM_001145000
VERSION     NM_001145000.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4074..4165 
                     /label="Myc-DDK"  
     misc_feature    4195..4780 
                     /label="PolyA signal"  
     misc_feature    4252..4271 
                     /label="Restriction Enzyme"  
     misc_feature    5056..5728 
                     /label="ColE1"  
     misc_feature    6158..6952 
                     /label="Kan/Neo"  
     misc_feature    6987..7289 
                     /label="SV40 ori"  
     misc_feature    7375..7852 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4056..4062 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4056 
                     /label="ITGAV"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTTCCG CCGCGGCGAC GGCTGCGCCT CGGTCCCCGC GGCCTCCCGC 
     1081 TTCTTCTCTC GGGACTCCTG CTACCTCTGT GCCGCGCCTT CAACCTAGAC GTGGACAGTC 
     1141 CTGCCGAGTA CTCTGGCCCC GAGGGAAGTT ACTTCGGCTT CGCCGTGGAT TTCTTCGTGC 
     1201 CCAGCGCGTC TTCCCGGATG TTTCTTCTCG TGGGAGCTCC CAAAGCAAAC ACCACCCAGC 
     1261 CTGGGATTGT GGAAGGAGGG CAGGTCCTCA AATGTGACTG GTCTTCTACC CGCCGGTGCC 
     1321 AGCCAATTGA ATTTGATGCA ACAGGCAATA GAGATTATGC CAAGGATGAT CCATTGGAAT 
     1381 TTAAGTCCCA TCAGTGGTTT GGAGCATCTG TGAGGTCGAA ACAGGATAAA ATTTTGGCCT 
     1441 GTGCCCCATT GTACCATTGG AGAACTGAGA TGAAACAGGA GCGAGAGCCT GTTGGAACAT 
     1501 GCTTTCTTCA AGATGGAACA AAGACTGTTG AGTATGCTCC ATGTAGATCA CGTCAGCTTA 
     1561 TTTCGGATCA AGTGGCAGAA ATCGTATCTA AATACGACCC CAATGTTTAC AGCATCAAGT 
     1621 ATAATAACCA ATTAGCAACT CGGACTGCAC AAGCTATTTT TGATGACAGC TATTTGGGTT 
     1681 ATTCTGTGGC TGTCGGAGAT TTCAATGGTG ATGGCATAGA TGACTTTGTT TCAGGAGTTC 
     1741 CAAGAGCAGC AAGGACTTTG GGAATGGTTT ATATTTATGA TGGGAAGAAC ATGTCCTCCT 
     1801 TATACAATTT TACTGGCGAG CAGATGGCTG CATATTTCGG ATTTTCTGTA GCTGCCACTG 
     1861 ACATTAATGG AGATGATTAT GCAGATGTGT TTATTGGAGC ACCTCTCTTC ATGGATCGTG 
     1921 GCTCTGATGG CAAACTCCAA GAGGTGGGGC AGGTCTCAGT GTCTCTACAG AGAGCTTCAG 
     1981 GAGACTTCCA GACGACAAAG CTGAATGGAT TTGAGGTCTT TGCACGGTTT GGCAGTGCCA 
     2041 TAGCTCCTTT GGGAGATCTG GACCAGGATG GTTTCAATGA TATTGCAATT GCTGCTCCAT 
     2101 ATGGGGGTGA AGATAAAAAA GGAATTGTTT ATATCTTCAA TGGAAGATCA ACAGGCTTGA 
     2161 ACGCAGTCCC ATCTCAAATC CTTGAAGGGC AGTGGGCTGC TCGAAGCATG CCACCAAGCT 
     2221 TTGGCTATTC AATGAAAGGA GCCACAGATA TAGACAAAAA TGGATATCCA GACTTAATTG 
     2281 TAGGAGCTTT TGGTGTAGAT CGAGCTATCT TATACAGGGC CAGACCAGTT ATCACTGTAA 
     2341 ATGCTGGTCT TGAAGTGTAC CCTAGCATTT TAAATCAAGA CAATAAAACC TGCTCACTGC 
     2401 CTGGAACAGC TCTCAAAGTT TCCTGTTTTA ATGTTAGGTT CTGCTTAAAG GCAGATGGCA 
     2461 AAGGAGTACT TCCCAGGAAA CTTAATTTCC AGGTGGAACT TCTTTTGGAT AAACTCAAGC 
     2521 AAAAGGGAGC AATTCGACGA GCACTGTTTC TCTACAGCAG GTCCCCAAGT CACTCCAAGA 
     2581 ACATGACTAT TTCAAGGGGG GGACTGATGC AGTGTGAGGA ATTGATAGCG TATCTGCGGG 
     2641 ATGAATCTGA ATTTAGAGAC AAACTCACTC CAATTACTAT TTTTATGGAA TATCGGTTGG 
     2701 ATTATAGAAC AGCTGCTGAT ACAACAGGCT TGCAACCCAT TCTTAACCAG TTCACGCCTG 
     2761 CTAACATTAG TCGACAGGCT CACATTCTAC TTGACTGTGG TGAAGACAAT GTCTGTAAAC 
     2821 CCAAGCTGGA AGTTTCTGTA GATAGTGATC AAAAGAAGAT CTATATTGGG GATGACAACC 
     2881 CTCTGACATT GATTGTTAAG GCTCAGAATC AAGGAGAAGG TGCCTACGAA GCTGAGCTCA 
     2941 TCGTTTCCAT TCCACTGCAG GCTGATTTCA TCGGGGTTGT CCGAAACAAT GAAGCCTTAG 
     3001 CAAGACTTTC CTGTGCATTT AAGACAGAAA ACCAAACTCG CCAGGTGGTA TGTGACCTTG 
     3061 GAAACCCAAT GAAGGCTGGA ACTCAACTCT TAGCTGGTCT TCGTTTCAGT GTGCACCAGC 
     3121 AGTCAGAGAT GGATACTTCT GTGAAATTTG ACTTACAAAT CCAAAGCTCA AATCTATTTG 
     3181 ACAAAGTAAG CCCAGTTGTA TCTCACAAAG TTGATCTTGC TGTTTTAGCT GCAGTTGAGA 
     3241 TAAGAGGAGT CTCGAGTCCT GATCATATCT TTCTTCCGAT TCCAAACTGG GAGCACAAGG 
     3301 AGAACCCTGA GACTGAAGAA GATGTTGGGC CAGTTGTTCA GCACATCTAT GAGCTGAGAA 
     3361 ACAATGGTCC AAGTTCATTC AGCAAGGCAA TGCTCCATCT TCAGTGGCCT TACAAATATA 
     3421 ATAATAACAC TCTGTTGTAT ATCCTTCATT ATGATATTGA TGGACCAATG AACTGCACTT 
     3481 CAGATATGGA GATCAACCCT TTGAGAATTA AGATCTCATC TTTGCAAACA ACTGAAAAGA 
     3541 ATGACACGGT TGCCGGGCAA GGTGAGCGGG ACCATCTCAT CACTAAGCGG GATCTTGCCC 
     3601 TCAGTGAAGG AGATATTCAC ACTTTGGGTT GTGGAGTTGC TCAGTGCTTG AAGATTGTCT 
     3661 GCCAAGTTGG GAGATTAGAC AGAGGAAAGA GTGCAATCTT GTACGTAAAG TCATTACTGT 
     3721 GGACTGAGAC TTTTATGAAT AAAGAAAATC AGAATCATTC CTATTCTCTG AAGTCGTCTG 
     3781 CTTCATTTAA TGTCATAGAG TTTCCTTATA AGAATCTTCC AATTGAGGAT ATCACCAACT 
     3841 CCACATTGGT TACCACTAAT GTCACCTGGG GCATTCAGCC AGCGCCCATG CCTGTGCCTG 
     3901 TGTGGGTGAT CATTTTAGCA GTTCTAGCAG GATTGTTGCT ACTGGCTGTT TTGGTATTTG 
     3961 TAATGTACAG GATGGGCTTT TTTAAACGGG TCCGGCCACC TCAAGAAGAA CAAGAAAGGG 
     4021 AGCAGCTTCA ACCTCATGAA AATGGTGAAG GAAACTCAGA AACTACGCGT ACGCGGCCGC 
     4081 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4141 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4201 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4261 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4321 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4381 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4441 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4501 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4561 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4621 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4681 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4741 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4801 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4861 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4921 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4981 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5041 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5101 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5161 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5221 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5281 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5341 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5401 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5461 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5521 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5581 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5641 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5701 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5761 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5821 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5881 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5941 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6001 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6061 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6121 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6181 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6241 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6301 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6361 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6421 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6481 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6541 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6601 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6661 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6721 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6781 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6841 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6901 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6961 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7021 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7081 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7141 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7201 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7261 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7321 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7381 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7441 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7501 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7561 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7621 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7681 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7741 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7801 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7861 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//