LOCUS       RC227574                8652 bp    DNA     circular  SYN 
DEFINITION  RASGRF1 (Myc-DDK-tagged)-Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 3
ACCESSION   NM_001145648
VERSION     NM_001145648.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4809..4900 
                     /label="Myc-DDK"  
     misc_feature    4930..5515 
                     /label="PolyA signal"  
     misc_feature    4987..5006 
                     /label="Restriction Enzyme"  
     misc_feature    5791..6463 
                     /label="ColE1"  
     misc_feature    6893..7687 
                     /label="Kan/Neo"  
     misc_feature    7722..8024 
                     /label="SV40 ori"  
     misc_feature    8110..8587 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4791..4797 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4791 
                     /label="RASGRF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAAGGGG ATCCGGCTGA ATGATGGCCA CGTCGCGTCC CTGGGACTGC 
     1081 TGGCGCGCAA GGACGGCACG CGCAAAGGCT ACCTGAGCAA GCGGAGTTCG GACAACACAA 
     1141 AATGGCAAAC CAAGTGGTTC GCGCTGCTGC AGAACCTGCT CTTCTACTTC GAGAGCGACT 
     1201 CGAGCTCGCG GCCCTCGGGG CTTTACCTGC TGGAGGGCTG CGTCTGCGAC CGCGCGCCCT 
     1261 CCCCCAAGCC GGCGCTGTCG GCCAAGGAGC CGCTGGAGAA ACAGCATTAC TTCACGGTGA 
     1321 ACTTCAGCCA TGAGAACCAG AAAGCCTTGG AGCTGAGGAC AGAGGACGCA AAAGATTGTG 
     1381 ACGAATGGGT GGCAGCCATT GCACATGCCA GCTACAGGAC CCTCGCCACA GAGCATGAGG 
     1441 CATTAATGCA GAAATACCTG CACCTGCTGC AGATCGTGGA GACAGAGAAG ACCGTGGCCA 
     1501 AGCAGCTTCG GCAGCAGATC GAGGATGGGG AGATCGAGAT CGAGCGGCTG AAGGCAGAGA 
     1561 TCACATCCCT GCTCAAGGAC AATGAGCGCA TCCAGTCCAC CCAGACTGTC GCCCCCAACG 
     1621 ATGAAGACAG CGACATCAAG AAAATTAAGA AGGTGCAGAG CTTCCTGCGG GGCTGGCTGT 
     1681 GCCGGCGGAA GTGGAAGACC ATCATCCAGG ACTACATCCG GTCACCCCAT GCTGACAGCA 
     1741 TGCGCAAGAG GAACCAGGTG GTGTTCAGCA TGCTGGAGGC TGAGGCTGAG TACGTGCAGC 
     1801 AGCTGCACAT CCTTGTCAAC AATTTCCTGC GCCCGCTGCG GATGGCCGCC AGCTCCAAGA 
     1861 AGCCTCCCAT CACACACGAC GACGTCAGCA GCATCTTCCT GAACAGCGAA ACCATCATGT 
     1921 TTTTACATCA GATCTTTTAC CAAGGCCTGA AGGCCCGCAT CTCCAGCTGG CCCACGCTGG 
     1981 TCCTGGCTGA CCTATTTGAC ATCCTGCTGC CCATGCTCAA CATCTACCAA GAGTTCGTCC 
     2041 GCAACCACCA GTACAGCCTG CAGATCCTGG CCCACTGCAA GCAGAACCGT GACTTCGACA 
     2101 AGCTGCTGAA GCACTACGAG GCCAAGCCTG ACTGCGAGGA GAGGACGCTG GAGACCTTCC 
     2161 TCACCTACCC CATGTTCCAG ATCCCCAGGT ACATCCTGAC CCTCCATGAG CTCCTGGCCC 
     2221 ACACGCCTCA TGAGCACGTT GAGCGCAACA GCCTGGACTA CGCCAAGTCC AAACTGGAGG 
     2281 AGCTGTCCAG AATAATGCAC GATGAAGTAA GTGAGACGGA GAACATCCGG AAAAACCTGG 
     2341 CCATCGAGCG CATGATCATC GAAGGCTGTG AGATCCTCCT GGACACCAGC CAGACCTTTG 
     2401 TGAGACAAGG TTCCCTCATT CAGGTGCCCA TGTCTGAAAA GGGCAAGATC ACCAGGGGGC 
     2461 GCCTGGGGTC TCTCTCCCTA AAGAAAGAGG GCGAGCGACA GTGCTTCCTG TTTTCTAAGC 
     2521 ATCTGATTAT CTGTACCAGA GGCTCTGGAG GGAAGCTTCA CTTGACCAAG AATGGAGTCA 
     2581 TATCCCTCAT TGACTGCACT TTATTGGAGG AGCCAGAAAG CACGGAGGAG GAAGCCAAAG 
     2641 GATCCGGCCA AGACATAGAT CACTTGGATT TTAAAATCGG GGTGGAGCCA AAGGATTCCC 
     2701 CGCCCTTTAC AGTCATCCTA GTGGCCTCGT CCAGACAGGA GAAGGCAGCG TGGACCAGTG 
     2761 ACATCAGCCA GTGTGTGGAT AACATCCGAT GCAATGGGCT CATGATGAAC GCATTTGAAG 
     2821 AAAATTCCAA GGTCACTGTG CCGCAGATGA TCAAGTCCGA CGCCTCCTTA TATTGTGATG 
     2881 ATGTTGACAT TCGCTTCAGC AAAACCATGA ACTCCTGCAA AGTGCTGCAG ATCCGCTACG 
     2941 CCAGTGTGGA GCGGCTGCTG GAGAGGCTGA CGGACCTGCG CTTCCTGAGC ATCGACTTCC 
     3001 TCAACACCTT CCTGCACTCC TACCGCGTCT TCACCACCGC CATCGTGGTC CTGGACAAGC 
     3061 TCATTACCAT CTACAAGAAG CCTATCAGTG CCATTCCTGC CAGGTCGCTG GAGCTCCTGT 
     3121 TTGCCAGTGG CCAGAACAAT AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA 
     3181 CCCGCAAGTT CTCCTCGCCG CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC 
     3241 GGCGGAAGCT CTCCCTGAAC ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG 
     3301 CCCTCAGCTG CAACTCCAAT GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA 
     3361 AGGCCACGCT GGACACCAGC AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG 
     3421 ATGAGGGCGA TACGACCCCT GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT 
     3481 CAGAAGTCTC CATGAGAGAG GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA 
     3541 CTGAAACATC ACCAACTAAA TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT 
     3601 CAGAGTTCCC ACTCTTTTCC TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG 
     3661 ACAATAACCG CAGTGCCTTG TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA 
     3721 ACGAGGGCAC CCCAAACAAG GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC 
     3781 CCCCAGACCA GAGGAATGGA GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG 
     3841 TCTTGAACGT GCTCCGCCAC TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG 
     3901 AGCTCAAATG CAAGGTGATC GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA 
     3961 CCCAGGAGCG GAAGGCTGCA GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG 
     4021 ACAACCAGAT CACGCTGGAG GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT 
     4081 TTGAAAACCA CTCAGCCCTG GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT 
     4141 TCAAGAAGAT TCCTTATGAG GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG 
     4201 AAAGGACCCC TTATATCATG AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG 
     4261 CTTCAGAAAT CATCCGCAAT GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG 
     4321 TGGCCGTAGC TGACATATGC CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT 
     4381 CGTCCATGAA CCGCAGTGCA ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC 
     4441 AGACTAAAGC TTTGATTGAT AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA 
     4501 ATCTCAGAGA AGCTCTGAAA AATTGTGACC CACCCTGTGT CCCTTACCTG GGGATGTACC 
     4561 TCACCGACCT GGCCTTCATC GAGGAGGGGA CGCCCAATTA CACGGAAGAC GGCCTGGTCA 
     4621 ACTTCTCCAA GATGAGGATG ATATCCCATA TTATCCGAGA GATTCGCCAG TTTCAACAAA 
     4681 CTGCCTACAA AATAGAGCAC CAAGCAAAGG TAACGCAATA TTTACTGGAC CAATCTTTTG 
     4741 TAATGGATGA AGAAAGCCTC TACGAGTCTT CTCTCCGAAT AGAACCAAAA CTCCCCACCA 
     4801 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4861 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4921 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4981 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5041 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5101 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5161 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5221 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5281 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5341 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5401 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5461 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5521 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5581 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5641 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5701 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5761 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5821 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5881 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5941 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6001 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6061 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6121 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6181 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6241 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6301 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6361 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6421 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6481 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6541 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6601 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6661 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6721 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6781 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6841 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6901 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6961 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7021 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7081 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7141 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7201 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7261 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7321 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7381 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7441 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7501 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7561 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7621 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7681 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7741 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7801 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7861 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8041 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8101 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8161 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8221 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8281 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8341 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8401 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8461 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8521 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8581 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8641 AACGCGAATT TT
//