LOCUS       RC227565                7725 bp    DNA     circular  SYN 
DEFINITION  NSMAF (Myc-DDK-tagged)-Human neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 2
ACCESSION   NM_001144772
VERSION     NM_001144772.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3882..3973 
                     /label="Myc-DDK"  
     misc_feature    4003..4588 
                     /label="PolyA signal"  
     misc_feature    4060..4079 
                     /label="Restriction Enzyme"  
     misc_feature    4864..5536 
                     /label="ColE1"  
     misc_feature    5966..6760 
                     /label="Kan/Neo"  
     misc_feature    6795..7097 
                     /label="SV40 ori"  
     misc_feature    7183..7660 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3864..3870 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3864 
                     /label="NSMAF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGGCCAG GCCAGATTCC ACCGCGGAGC CGAGGCATGC CGAGCTATCC 
     1081 GGCAGTGCAG GTCCCGGCGG CCGCGGGCAG CTCACCCGGA AGGCCGCGCG GGGTCCGGGC 
     1141 GTGGATCCCG GCACGCGAGC CCGGGCGTTC GCAGGGGAGG ATTTTCCTTG CTGCTGCTTA 
     1201 ACTTGGAGGA GTACTACTTT GAACAGCATA GAGCCAATCA CATTTTGCAC AAGGGCAGTC 
     1261 ACCATGAAAG GAAAATCAGA GGCTCCTTAA AAATATGTTC AAAATCGGTG ATTTTTGAAC 
     1321 CAGATTCAAT ATCCCAGCCC ATCATCAAGA TTCCTTTGAG AGACTGTATA AAAATAGGAA 
     1381 AGCATGGAGA AAATGGAGCC AATAGACACT TCACAAAGGC AAAATCTGGG GGTATTTCAC 
     1441 TCATTTTCAG TCAGGTATAT TTCATTAAAG AACATAATGT TGTTGCACCA TATAAAATAG 
     1501 AAAGGGGCAA AATGGAATAT GTTTTTGAAT TGGATGTTCC CGGGAAAGTG GAAGATGTTG 
     1561 TGGAGACGTT GCTTCAGCTT CACAGAGCAT CCTGCCTTGA CAAATTGGGT GACCAAACCG 
     1621 CCATGATAAC AGCTATTTTG CAGTCTCGTT TAGCTAGAAC ATCATTTGAC AAAAACAGGT 
     1681 TCCAAAACAT TTCTGAAAAG CTGCACATGG AATGCAAAGC AGAAATGGTG ACGCCTCTGG 
     1741 TGACTAATCC TGGACACGTG TGCATCACGG ACACAAACCT GTATTTTCAG CCCCTCAACG 
     1801 GCTACCCGAA ACCTGTGGTC CAGATAACAC TCCAAGATGT CCGCCGCATC TACAAAAGGA 
     1861 GGCACGGCCT CATGCCTCTG GGCTTGGAAG TATTTTGCAC AGAAGATGAT CTGTGTTCCG 
     1921 ACATCTACCT AAAGTTCTAT GAACCTCAAG ATAGAGATGA TCTCTATTTT TACATTGCCA 
     1981 CATACCTAGA GCACCATGTG GCGGAGCACA CTGCTGAGAG CTACATGCTG CAGTGGCAGC 
     2041 GTGGACACCT TTCCAACTAT CAGTACCTCC TTCACCTCAA CAACCTGGCC GACCGCAGCT 
     2101 GCAACGACCT CTCCCAGTAC CCTGTGTTTC CATGGATAAT ACATGATTAT TCCAGCTCAG 
     2161 AACTAGATTT GTCAAATCCA GGAACCTTCC GGGATCTCAG TAAGCCAGTA GGGGCCCTAA 
     2221 ATAAGGAACG GCTGGAGAGA CTACTGACAC GCTACCAGGA AATGCCTGAA CCAAAGTTCA 
     2281 TGTATGGGAG TCACTACTCT TCCCCGGGTT ATGTACTTTT TTATCTTGTT AGGATTGCAC 
     2341 CAGAGTATAT GCTGTGCCTG CAGAATGGAA GATTTGATAA TGCAGATAGA ATGTTCAACA 
     2401 GTATTGCAGA AACTTGGAAA AACTGTCTGG ATGGTGCAAC GGATTTTAAA GAGTTAATTC 
     2461 CAGAATTCTA TGGTGATGAT GTGAGCTTTC TAGTCAATAG CCTGAAGTTG GATTTGGGAA 
     2521 AGAGACAAGG AGGACAGATG GTTGACGACG TGGAGCTTCC CCCTTGGGCT TCCAGTCCCG 
     2581 AGGACTTTCT CCAGAAGAGC AAAGATGCAT TGGAAAGCAA TTATGTGTCT GAACACCTTC 
     2641 ACGAGTGGAT TGATCTAATA TTTGGCTACA AACAAAAAGG GAGTGATGCA GTTGGGGCCC 
     2701 ATAATGTATT TCATCCCCTG ACCTATGAAG GAGGTGTAGA CTTGAACAGC ATCCAGGATC 
     2761 CTGATGAGAA GGTAGCCATG CTTACGCAAA TCTTGGAATT TGGGCAGACA CCAAAACAAC 
     2821 TATTTGTGAC ACCACATCCT CGAAGGATCA CCCCAAAGTT TAAAAGTTTG TCCCAGACCT 
     2881 CCAGTTATAA TGCTTCTATG GCAGATTCCC CAGGTGAAGA GTCTTTTGAA GACCTGACCG 
     2941 AAGAAAGCAA AACACTGGCC TGGAATAACA TCACCAAACT GCAGTTACAC GAGCACTATA 
     3001 AAATCCACAA AGAAGCAGTT ACTGGAATCA CGGTCTCTCG CAATGGATCT TCAGTATTCA 
     3061 CAACATCCCA AGATTCCACC TTGAAGATGT TTTCTAAAGA ATCAAAAATG CTACAAAGAA 
     3121 GTATATCATT TTCAAATATG GCTTTATCGT CTTGTTTACT TTTACCAGGA GATGCCACTG 
     3181 TCATAACTTC TTCATGGGAT AATAATGTCT ATTTTTATTC CATAGCATTT GGAAGACGCC 
     3241 AGGACACGTT AATGGGACAT GATGATGCTG TTAGTAAGAT CTGTTGGCAT GACAACAGGC 
     3301 TATATTCTGC ATCGTGGGAC TCTACAGTGA AGGTGTGGTC TGGTGTTCCT GCAGAGATGC 
     3361 CAGGCACCAA AAGACACCAC TTTGACTTGC TGGCCGAGCT GGAACATGAT GTCAGTGTAG 
     3421 ATACAATCAG TTTAAATGCT GCAAGCACAC TGTTAGTTTC CGGCACCAAA GAAGGCACAG 
     3481 TGAATATTTG GGACCTCACA ACGGCCACCT TAATGCACCA GATTCCATGC CATTCAGGGA 
     3541 TTGTATGTGA CACTGCTTTT AGCCCAGATA GTCGCCATGT CCTCAGCACA GGAACAGATG 
     3601 GCTGTCTTAA TGTCATTGAT GTGCAGACAG GAATGCTCAT CTCCTCCATG ACATCAGATG 
     3661 AGCCCCAGAG GTGCTTTGTC TGGGATGGAA ATTCCGTTTT ATCTGGCAGT CAGTCTGGTG 
     3721 AACTGCTCGT TTGGGACCTC CTTGGAGCAA AAATCAGTGA GAGAATACAG GGCCACACAG 
     3781 GTGCTGTGAC ATGTATATGG ATGAATGAAC AGTGTAGCAG TATCATCACA GGAGGGGAAG 
     3841 ACAGACAAAT TATATTCTGG AAATTGCAGT ATACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3901 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3961 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4021 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4081 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4141 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4201 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4261 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4321 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4381 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4441 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4501 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4561 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4621 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4681 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4741 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4801 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4861 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4921 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4981 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5041 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5101 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5161 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5221 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5281 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5341 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5401 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5461 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5521 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5581 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5641 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5701 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5761 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5821 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5881 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5941 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6001 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6061 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6121 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6181 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6241 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6301 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6361 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6421 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6481 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6541 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6601 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6661 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6721 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6781 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6841 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6901 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6961 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7021 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7081 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7141 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7201 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7261 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7321 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7381 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7441 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7501 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7561 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7621 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7681 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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