LOCUS RC227530 7416 bp DNA circular SYN DEFINITION SLITRK2 (Myc-DDK-tagged)-Human SLIT and NTRK-like family, member 2 (SLITRK2), transcript variant 8 ACCESSION NM_001144009 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3573..3664 /label="Myc-DDK" misc_feature 3694..4279 /label="PolyA signal" misc_feature 3751..3770 /label="Restriction Enzyme" misc_feature 4555..5227 /label="ColE1" misc_feature 5657..6451 /label="Kan/Neo" misc_feature 6486..6788 /label="SV40 ori" misc_feature 6874..7351 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3555..3561 /label="Restriction Enzyme" misc_feature 1020..3555 /label="SLITRK2" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GCTGAGCGGC GTTTGGTTCC TCAGTGTGTT AACCGTGGCC GGGATCTTAC 1081 AGACAGAGAG TCGCAAAACT GCCAAAGACA TTTGCAAGAT CCGCTGTCTG TGCGAAGAAA 1141 AGGAAAACGT ACTGAATATC AACTGTGAGA ACAAAGGATT TACAACAGTT AGCCTGCTCC 1201 AGCCCCCCCA GTATCGAATC TATCAGCTTT TTCTCAATGG AAACCTCTTG ACAAGACTGT 1261 ATCCAAACGA ATTTGTCAAT TACTCCAACG CGGTGACTCT TCACCTAGGT AACAACGGGT 1321 TACAGGAGAT CCGAACGGGG GCATTCAGTG GCCTGAAAAC TCTCAAAAGA CTGCATCTCA 1381 ACAACAACAA GCTTGAGATA TTGAGGGAGG ACACCTTCCT AGGCCTGGAG AGCCTGGAGT 1441 ATCTCCAGGC CGACTACAAT TACATCAGTG CCATCGAGGC TGGGGCATTC AGCAAACTTA 1501 ACAAGCTCAA AGTGCTCATC CTGAATGACA ACCTTCTGCT TTCACTGCCC AGCAATGTGT 1561 TCCGCTTTGT CCTGCTGACC CACTTAGACC TCAGGGGGAA TAGGCTAAAA GTAATGCCTT 1621 TTGCTGGCGT CCTTGAACAT ATTGGAGGGA TCATGGAGAT TCAGCTGGAG GAAAATCCAT 1681 GGAATTGCAC TTGTGACTTA CTTCCTCTCA AGGCCTGGCT AGACACCATA ACTGTTTTTG 1741 TGGGAGAGAT TGTCTGTGAG ACTCCCTTTA GGTTGCATGG GAAAGACGTG ACCCAGCTGA 1801 CCAGGCAAGA CCTCTGTCCC AGAAAAAGTG CCAGTGATTC CAGTCAGAGG GGCAGCCATG 1861 CTGACACCCA CGTCCAAAGG CTGTCACCTA CAATGAATCC TGCTCTCAAC CCAACCAGGG 1921 CTCCGAAAGC CAGCCGGCCG CCCAAAATGA GAAATCGTCC AACTCCCCGA GTGACTGTGT 1981 CAAAGGACAG GCAAAGTTTT GGACCCATCA TGGTGTACCA GACCAAGTCT CCTGTGCCTC 2041 TCACCTGTCC CAGCAGCTGT GTCTGCACCT CTCAGAGCTC AGACAATGGT CTGAATGTAA 2101 ACTGCCAAGA AAGGAAGTTC ACTAATATCT CTGACCTGCA GCCCAAACCG ACCAGTCCAA 2161 AGAAACTCTA CCTAACAGGG AACTATCTTC AAACTGTCTA TAAGAATGAC CTCTTAGAAT 2221 ACAGTTCTTT GGACTTACTG CACTTAGGAA ACAACAGGAT TGCAGTCATT CAGGAAGGTG 2281 CCTTTACAAA CCTGACCAGT TTACGCAGAC TTTATCTGAA TGGCAATTAC CTTGAAGTGC 2341 TGTACCCTTC TATGTTTGAT GGACTGCAGA GCTTGCAATA TCTCTATTTA GAGTATAATG 2401 TCATTAAGGA AATTAAGCCT CTGACCTTTG ATGCTTTGAT TAACCTACAG CTACTGTTTC 2461 TGAACAACAA CCTTCTTCGG TCCTTACCTG ATAATATATT TGGGGGGACG GCCCTAACCA 2521 GGCTGAATCT GAGAAACAAC CATTTTTCTC ACCTGCCCGT GAAAGGGGTT CTGGATCAGC 2581 TCCCGGCTTT CATCCAGATA GATCTGCAGG AGAACCCCTG GGACTGTACC TGTGACATCA 2641 TGGGGCTGAA AGACTGGACA GAACATGCCA ATTCCCCTGT CATCATTAAT GAGGTGACTT 2701 GCGAATCTCC TGCTAAGCAT GCAGGGGAGA TACTAAAATT TCTGGGGAGG GAGGCTATCT 2761 GTCCAGACAG CCCAAACTTG TCAGATGGAA CCGTCTTGTC AATGAATCAC AATACAGACA 2821 CACCTCGGTC GCTTAGTGTG TCTCCTAGTT CCTATCCTGA ACTACACACT GAAGTTCCAC 2881 TGTCTGTCTT AATTCTGGGA TTGCTTGTTG TTTTCATCTT ATCTGTCTGT TTTGGGGCTG 2941 GTTTATTCGT CTTTGTCTTG AAACGCCGAA AGGGAGTGCC GAGCGTTCCC AGGAATACCA 3001 ACAACTTAGA CGTAAGCTCC TTTCAATTAC AGTATGGGTC TTACAACACT GAGACTCACG 3061 ATAAAACAGA CGGCCATGTC TACAACTATA TCCCCCCACC TGTGGGTCAG ATGTGCCAAA 3121 ACCCCATCTA CATGCAGAAG GAAGGAGACC CAGTAGCCTA TTACCGAAAC CTGCAAGAGT 3181 TCAGCTATAG CAACCTGGAG GAGAAAAAAG AAGAGCCAGC CACACCTGCT TACACAATAA 3241 GTGCCACTGA GCTGCTAGAA AAGCAGGCCA CACCAAGAGA GCCTGAGCTG CTGTATCAAA 3301 ATATTGCTGA GCGAGTCAAG GAACTTCCCA GCGCAGGCCT AGTCCACTAT AACTTTTGTA 3361 CCTTACCTAA AAGGCAGTTT GCCCCTTCCT ATGAATCTCG ACGCCAAAAC CAAGACAGAA 3421 TCAATAAAAC CGTTTTATAT GGAACTCCCA GGAAATGCTT TGTGGGGCAG TCAAAACCCA 3481 ACCACCCTTT ACTGCAAGCT AAGCCGCAAT CAGAACCGGA CTACCTCGAA GTTCTGGAAA 3541 AACAAACTGC AATCAGTCAG CTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 3601 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 3661 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 3721 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 3781 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 3841 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 3901 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 3961 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 4021 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 4081 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 4141 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 4201 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 4261 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 4321 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 4381 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 4441 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 4501 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 4561 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 4621 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 4681 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 4741 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 4801 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 4861 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 4921 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 4981 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 5041 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 5101 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 5161 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 5221 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 5281 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 5341 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 5401 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 5461 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 5521 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 5581 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 5641 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 5701 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 5761 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 5821 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 5881 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 5941 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 6001 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 6061 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 6121 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 6181 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 6241 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 6301 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 6361 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 6421 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 6481 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 6541 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 6601 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 6661 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 6721 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 6781 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 6841 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 6901 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 6961 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 7021 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 7081 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 7141 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 7201 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 7261 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 7321 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 7381 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT //