LOCUS       RC227350                8625 bp    DNA     circular  SYN 
DEFINITION  L1CAM (Myc-DDK-tagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 3
ACCESSION   NM_001143963
VERSION     NM_001143963.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4782..4873 
                     /label="Myc-DDK"  
     misc_feature    4903..5488 
                     /label="PolyA signal"  
     misc_feature    4960..4979 
                     /label="Restriction Enzyme"  
     misc_feature    5764..6436 
                     /label="ColE1"  
     misc_feature    6866..7660 
                     /label="Kan/Neo"  
     misc_feature    7695..7997 
                     /label="SV40 ori"  
     misc_feature    8083..8560 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4764..4770 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4764 
                     /label="L1CAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCGTGGCG CTGCGGTACG TGTGGCCTCT CCTCCTCTGC AGCCCCTGCC 
     1081 TGCTTATCCA GATCCCCGAG GAATTGATGG AGCCACCTGT CATCACGGAA CAGTCTCCAC 
     1141 GGCGCCTGGT TGTCTTCCCC ACAGATGACA TCAGCCTCAA GTGTGAGGCC AGTGGCAAGC 
     1201 CCGAAGTGCA GTTCCGCTGG ACGAGGGATG GTGTCCACTT CAAACCCAAG GAAGAGCTGG 
     1261 GTGTGACCGT GTACCAGTCG CCCCACTCTG GCTCCTTCAC CATCACGGGC AACAACAGCA 
     1321 ACTTTGCTCA GAGGTTCCAG GGCATCTACC GCTGCTTTGC CAGCAATAAG CTGGGCACCG 
     1381 CCATGTCCCA TGAGATCCGG CTCATGGCCG AGGGTGCCCC CAAGTGGCCA AAGGAGACAG 
     1441 TGAAGCCCGT GGAGGTGGAG GAAGGGGAGT CAGTGGTTCT GCCTTGCAAC CCTCCCCCAA 
     1501 GTGCAGAGCC TCTCCGGATC TACTGGATGA ACAGCAAGAT CTTGCACATC AAGCAGGACG 
     1561 AGCGGGTGAC GATGGGCCAG AACGGCAACC TCTACTTTGC CAATGTGCTC ACCTCCGACA 
     1621 ACCACTCAGA CTACATCTGC CACGCCCACT TCCCAGGCAC CAGGACCATC ATTCAGAAGG 
     1681 AACCCATTGA CCTCCGGGTC AAGGCCACCA ACAGCATGAT TGACAGGAAG CCGCGCCTGC 
     1741 TCTTCCCCAC CAACTCCAGC AGCCACCTGG TGGCCTTGCA GGGGCAGCCA TTGGTCCTGG 
     1801 AGTGCATCGC CGAGGGCTTT CCCACGCCCA CCATCAAATG GCTGCGCCCC AGTGGCCCCA 
     1861 TGCCAGCCGA CCGTGTCACC TACCAGAACC ACAACAAGAC CCTGCAGCTG CTGAAAGTGG 
     1921 GCGAGGAGGA TGATGGCGAG TACCGCTGCC TGGCCGAGAA CTCACTGGGC AGTGCCCGGC 
     1981 ATGCGTACTA TGTCACCGTG GAGGCTGCCC CGTACTGGCT GCACAAGCCC CAGAGCCATC 
     2041 TATATGGGCC AGGAGAGACT GCCCGCCTGG ACTGCCAAGT CCAGGGCAGG CCCCAACCAG 
     2101 AGGTCACCTG GAGAATCAAC GGGATCCCTG TGGAGGAGCT GGCCAAAGAC CAGAAGTACC 
     2161 GGATTCAGCG TGGCGCCCTG ATCCTGAGCA ACGTGCAGCC CAGTGACACA ATGGTGACCC 
     2221 AATGTGAGGC CCGCAACCGG CACGGGCTCT TGCTGGCCAA TGCCTACATC TACGTTGTCC 
     2281 AGCTGCCAGC CAAGATCCTG ACTGCGGACA ATCAGACGTA CATGGCTGTC CAGGGCAGCA 
     2341 CTGCCTACCT TCTGTGCAAG GCCTTCGGAG CGCCTGTGCC CAGTGTTCAG TGGCTGGACG 
     2401 AGGATGGGAC AACAGTGCTT CAGGACGAAC GCTTCTTCCC CTATGCCAAT GGGACCCTGG 
     2461 GCATTCGAGA CCTCCAGGCC AATGACACCG GACGCTACTT CTGCCTGGCT GCCAATGACC 
     2521 AAAACAATGT TACCATCATG GCTAACCTGA AGGTTAAAGA TGCAACTCAG ATCACTCAGG 
     2581 GGCCCCGCAG CACAATCGAG AAGAAAGGTT CCAGGGTGAC CTTCACGTGC CAGGCCTCCT 
     2641 TTGACCCCTC CTTGCAGCCC AGCATCACCT GGCGTGGGGA CGGTCGAGAC CTCCAGGAGC 
     2701 TTGGGGACAG TGACAAGTAC TTCATAGAGG ATGGGCGCCT GGTCATCCAC AGCCTGGACT 
     2761 ACAGCGACCA GGGCAACTAC AGCTGCGTGG CCAGTACCGA ACTGGATGTG GTGGAGAGTA 
     2821 GGGCACAGCT CTTGGTGGTG GGGAGCCCTG GGCCGGTGCC ACGGCTGGTG CTGTCCGACC 
     2881 TGCACCTGCT GACGCAGAGC CAGGTGCGCG TGTCCTGGAG TCCTGCAGAA GACCACAATG 
     2941 CCCCCATTGA GAAATATGAC ATTGAATTTG AGGACAAGGA AATGGCGCCT GAAAAATGGT 
     3001 ACAGTCTGGG CAAGGTTCCA GGGAACCAGA CCTCTACCAC CCTCAAGCTG TCGCCCTATG 
     3061 TCCACTACAC CTTTAGGGTT ACTGCCATAA ACAAATATGG CCCCGGGGAG CCCAGCCCGG 
     3121 TCTCTGAGAC TGTGGTCACA CCTGAGGCAG CCCCAGAGAA GAACCCTGTG GATGTGAAGG 
     3181 GGGAAGGAAA TGAGACCACC AATATGGTCA TCACGTGGAA GCCGCTCCGG TGGATGGACT 
     3241 GGAACGCCCC CCAGGTTCAG TACCGCGTGC AGTGGCGCCC TCAGGGGACA CGAGGGCCCT 
     3301 GGCAGGAGCA GATTGTCAGC GACCCCTTCC TGGTGGTGTC CAACACGTCC ACCTTCGTGC 
     3361 CCTATGAGAT CAAAGTCCAG GCCGTCAACA GCCAGGGCAA GGGACCAGAG CCCCAGGTCA 
     3421 CTATCGGCTA CTCTGGAGAG GACTACCCCC AGGCAATCCC TGAGCTGGAA GGCATTGAAA 
     3481 TCCTCAACTC AAGTGCCGTG CTGGTCAAGT GGCGGCCGGT GGACCTGGCC CAGGTCAAGG 
     3541 GCCACCTCCG CGGATACAAT GTGACGTACT GGAGGGAGGG CAGTCAGAGG AAGCACAGCA 
     3601 AGAGACATAT CCACAAAGAC CATGTGGTGG TGCCCGCCAA CACCACCAGT GTCATCCTCA 
     3661 GTGGCTTGCG GCCCTATAGC TCCTACCACC TGGAGGTGCA GGCCTTTAAC GGGCGAGGAT 
     3721 CGGGGCCCGC CAGCGAGTTC ACCTTCAGCA CCCCAGAGGG AGTGCCTGGC CACCCCGAGG 
     3781 CGTTGCACCT GGAGTGCCAG TCGAACACCA GCCTGCTGCT GCGCTGGCAG CCCCCACTCA 
     3841 GCCACAACGG CGTGCTCACC GGCTACGTGC TCTCCTACCA CCCCCTGGAT GAGGGGGGCA 
     3901 AGGGGCAACT GTCCTTCAAC CTTCGGGACC CCGAACTTCG GACACACAAC CTGACCGATC 
     3961 TCAGCCCCCA CCTGCGGTAC CGCTTCCAGC TTCAGGCCAC CACCAAAGAG GGCCCTGGTG 
     4021 AAGCCATCGT ACGGGAAGGA GGCACTATGG CCTTGTCTGG GATCTCAGAT TTTGGCAACA 
     4081 TCTCAGCCAC AGCGGGTGAA AACTACAGTG TCGTCTCCTG GGTCCCCAAG GAGGGCCAGT 
     4141 GCAACTTCAG GTTCCATATC TTGTTCAAAG CCTTGGGAGA AGAGAAGGGT GGGGCTTCCC 
     4201 TTTCGCCACA GTATGTCAGC TACAACCAGA GCTCCTACAC GCAGTGGGAC CTGCAGCCTG 
     4261 ACACTGACTA CGAGATCCAC TTGTTTAAGG AGAGGATGTT CCGGCACCAA ATGGCTGTGA 
     4321 AGACCAATGG CACAGGCCGC GTGAGGCTCC CTCCTGCTGG CTTCGCCACT GAGGGCTGGT 
     4381 TCATCGGCTT TGTGAGTGCC ATCATCCTCC TGCTCCTCGT CCTGCTCATC CTCTGCTTCA 
     4441 TCAAGCGCAG CAAGGGCGGC AAATACTCAG TGAAGGATAA GGAGGACACC CAGGTGGACT 
     4501 CTGAGGCCCG ACCGATGAAA GATGAGACCT TCGGCGAGTA CAGTGACAAC GAGGAGAAGG 
     4561 CCTTTGGCAG CAGCCAGCCA TCGCTCAACG GGGACATCAA GCCCCTGGGC AGTGACGACA 
     4621 GCCTGGCCGA TTATGGGGGC AGCGTGGATG TTCAGTTCAA CGAGGATGGT TCGTTCATTG 
     4681 GCCAGTACAG TGGCAAGAAG GAGAAGGAGG CGGCAGGGGG CAATGACAGC TCAGGGGCCA 
     4741 CTTCCCCCAT CAACCCTGCC GTGGCCCTAG AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4801 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4861 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4921 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4981 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5041 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5101 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5161 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5221 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5281 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5341 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5401 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5461 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5521 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5581 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5641 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5701 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5761 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5821 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5881 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5941 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6001 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6061 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6121 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6181 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6241 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6301 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6361 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6421 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6481 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6541 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6601 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6661 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6721 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6781 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6841 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6901 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6961 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7021 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7081 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7141 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7201 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7261 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7321 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7381 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7441 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7501 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7561 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7621 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7681 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7741 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7801 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7861 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8041 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8101 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8161 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8221 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8281 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8341 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8401 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8461 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8521 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8581 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//