LOCUS       RC227297                7989 bp    DNA     circular  SYN 
DEFINITION  ULK2 (Myc-DDK-tagged)-Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 2
ACCESSION   NM_001142610
VERSION     NM_001142610.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4146..4237 
                     /label="Myc-DDK"  
     misc_feature    4267..4852 
                     /label="PolyA signal"  
     misc_feature    4324..4343 
                     /label="Restriction Enzyme"  
     misc_feature    5128..5800 
                     /label="ColE1"  
     misc_feature    6230..7024 
                     /label="Kan/Neo"  
     misc_feature    7059..7361 
                     /label="SV40 ori"  
     misc_feature    7447..7924 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4128..4134 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4128 
                     /label="ULK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGTGGTG GGTGACTTCG AGTACAGCAA GAGGGATCTC GTGGGACACG 
     1081 GGGCCTTCGC CGTGGTCTTC CGGGGGCGGC ACCGCCAGAA AACTGATTGG GAGGTAGCTA 
     1141 TTAAAAGTAT TAATAAAAAG AACTTGTCAA AATCACAAAT ACTGCTTGGA AAGGAAATTA 
     1201 AAATCTTAAA GGAACTTCAG CATGAAAATA TTGTAGCACT CTATGATGTT CAGGAATTAC 
     1261 CCAACTCTGT CTTTTTGGTG ATGGAGTATT GCAATGGTGG AGACCTCGCA GATTATTTGC 
     1321 AAGCGAAAGG GACTCTCAGT GAAGACACGA TCAGAGTGTT TCTGCATCAG ATTGCTGCTG 
     1381 CCATGCGAAT CCTGCACAGC AAAGGAATCA TCCACAGAGA TCTCAAACCA CAGAACATCT 
     1441 TGCTGTCCTA TGCCAATCGC AGAAAATCAA GTGTCAGTGG TATTCGCATC AAAATAGCGG 
     1501 ATTTTGGTTT TGCTCGTTAC CTACATAGTA ACATGATGGC TGCAACACTG TGTGGATCCC 
     1561 CGATGTACAT GGCTCCTGAG GTTATTATGT CTCAACATTA TGATGCTAAG GCTGACTTGT 
     1621 GGAGCATAGG AACAGTGATA TACCAATGCC TAGTTGGAAA ACCACCTTTT CAGGCCAATA 
     1681 GTCCTCAAGA CTTAAGGATG TTTTATGAAA AAAACAGGAG CTTAATGCCT AGTATTCCCA 
     1741 GAGAAACATC ACCTTATTTG GCTAATCTCC TTTTGGGTTT GCTTCAGAGA AACCAAAAAG 
     1801 ATAGAATGGA CTTTGAAGCG TTTTTTAGCC ATCCTTTTCT TGAGCAAGGT CCAGTAAAAA 
     1861 AATCTTGCCC AGTTCCAGTG CCCATGTATT CTGGTTCTGT CTCTGGAAGC TCCTGTGGCA 
     1921 GCTCTCCATC TTGTCGTTTT GCTTCTCCAC CATCCCTTCC AGATATGCAG CATATTCAGG 
     1981 AAGAAAACTT ATCTTCCCCA CCATTGGGTC CTCCCAACTA TCTACAAGTT TCCAAAGATT 
     2041 CTGCCAGTAC TAGTAGCAAG AACTCTTCTT GTGACACGGA TGACTTTGTT TTGGTGCCAC 
     2101 ACAACATCTC GTCAGACCAC TCATGTGATA TGCCAATGGG GACTGCTGGC AGACGTGCTT 
     2161 CAAATGAATT CTTGGTGTGT GGAGGGCAGT GTCAGCCTAC TGTGTCACCT CACAGCGAAA 
     2221 CAGCACCAAT TCCAGTTCCT ACTCAAATAA GGAATTATCA GCGCATAGAG CAGAATCTTA 
     2281 CATCTACTGC CAGCTCAGGC ACAAATGTAC ATGGTTCTCC AAGATCTGCA GTGGTACGAA 
     2341 GGTCCAACAC CAGCCCCATG GGCTTCCTCC GGCCGGGATC ATGCTCCCCA GTACCAGCAG 
     2401 ACACAGCACA GACAGTTGGA CGAAGGCTCT CCACTGGGTC TTCTAGGCCT TACTCACCTT 
     2461 CCCCTTTGGT TGGTACCATT CCTGAGCAAT TCAGTCAGTG CTGCTGTGGG CATCCTCAGG 
     2521 GCCATGACTC CAGGAGTAGA AACTCCTCAG GTTCTCCAGT GCCACAAGCT CAGTCCCCAC 
     2581 AGTCTCTCTT ATCGGGTGCT AGACTGCAGA GCGCCCCCAC CCTCACTGAC ATCTATCAGA 
     2641 ACAAGCAGAA GCTCAGAAAA CAGCACTCTG ACCCCGTGTG CCCATCCCAT ACTGGGGCTG 
     2701 GGTACAGCTA CTCGCCTCAG CCCAGTCGGC CTGGCAGCCT TGGAACTTCT CCCACCAAGC 
     2761 ACTTGGGGTC CTCTCCACGG AGTTCTGACT GGTTCTTTAA AACTCCTTTG CCAACAATCA 
     2821 TTGGCTCTCC TACTAAGACC ACAGCTCCTT TCAAAATCCC TAAAACTCAA GCATCTTCCA 
     2881 ACCTGTTAGC CTTGGTTACT CGTCATGGGC CTGCTGAAGA ACAGTCGAAA GATGGGAATG 
     2941 AGCCACGGGA ATGTGCCCAT TGCCTCTTAG TGCAAGGAAG TGAGAGGCAG CGGGCCGAGC 
     3001 AGCAGAGCAA GGCAGTGTTT GGCAGATCTG TCAGTACCGG GAAGTTATCA GATCAACAAG 
     3061 GAAAGACTCC TATATGTCGA CATCAGGGCA GCACAGACAG TTTAAATACA GAACGACCAA 
     3121 TGGATATAGC TCCGGCAGGA GCCTGTGGTG GTGTTCTGGC ACCTCCTGCA GGTACAGCAG 
     3181 CAAGTTCCAA GGCTGTCCTC TTCACTGTAG GGTCTCCTCC ACACAGTGCG GCAGCCCCCA 
     3241 CTTGTACCCA CATGTTCCTT CGAACAAGAA CAACCTCAGT GGGGCCCAGC AACTCCGGGG 
     3301 GCTCTCTTTG TGCCATGAGT GGCCGCGTGT GCGTGGGGTC CCCGCCTGGC CCAGGCTTCG 
     3361 GCTCTTCCCC TCCAGGAGCA GAGGCAGCTC CCAGCCTGAG ATACGTGCCT TACGGTGCTT 
     3421 CACCCCCCAG CCTAGAGGGG CTCATCACCT TTGAAGCCCC TGAACTGCCG GAGGAGACGC 
     3481 TGATGGAGCG GGAACACACA GACACCTTAC GCCATCTGAA TGTGATGCTG ATGTTCACTG 
     3541 AGTGTGTGCT GGACCTGACA GCCATGAGGG GAGGAAACCC TGAGCTGTGC ACATCTGCTG 
     3601 TGTCCTTGTA CCAGATCCAG GAGAGTGTGG TGGTGGACCA GATCAGTCAG CTGAGCAAAG 
     3661 ACTGGGGGCG GGTGGAGCAG CTGGTGTTGT ACATGAAAGC AGCACAGCTG CTTGCGGCTT 
     3721 CTCTGCATCT TGCCAAAGCC CAGATCAAGT CCGGGAAACT GAGCCCATCC ACAGCTGTGA 
     3781 AACAAGTTGT CAAGAATCTG AACGAACGAT ATAAATTCTG CATCACCATG TGCAAGAAAC 
     3841 TTACAGAAAA GCTGAATCGA TTCTTCTCTG ACAAACAGAG GTTTATTGAT GAAATCAACA 
     3901 GTGTGACTGC AGAGAAACTC ATCTATAATT GTGCTGTAGA AATGGTTCAG TCTGCAGCCC 
     3961 TGGATGAGAT GTTTCAGCAG ACCGAAGATA TTGTTTATCG CTATCATAAG GCAGCCCTTC 
     4021 TTTTGGAAGG CCTAAGTAGG ATTCTACAGG ACCCTGCAGA TATTGAAAAT GTGCATAAAT 
     4081 ATAAATGTAG TATTGAGAGA AGACTGTCGG CGCTCTGCCA TAGCACCGCA ACCGTGACGC 
     4141 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4201 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4261 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4321 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4381 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4441 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4501 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4561 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4621 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4681 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4741 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4801 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4861 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4921 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4981 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5041 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5101 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5161 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5221 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5281 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5341 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5401 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5461 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5521 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5581 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5641 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5701 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5761 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5821 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5881 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5941 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6001 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6061 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6121 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6181 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6241 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6301 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6361 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6421 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6481 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6541 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6601 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6661 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6721 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6781 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6841 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6901 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6961 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7021 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7081 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7141 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7201 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7261 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7321 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7381 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7441 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7501 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7561 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7621 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7681 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7741 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7801 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7861 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7921 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7981 GCGAATTTT
//