LOCUS       RC227260                7128 bp    DNA     circular  SYN 
DEFINITION  DLG2 (Myc-DDK-tagged)-Human discs, large homolog 2 (Drosophila) (DLG2), transcript variant 3
ACCESSION   NM_001142700
VERSION     NM_001142700.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3285..3376 
                     /label="Myc-DDK"  
     misc_feature    3406..3991 
                     /label="PolyA signal"  
     misc_feature    3463..3482 
                     /label="Restriction Enzyme"  
     misc_feature    4267..4939 
                     /label="ColE1"  
     misc_feature    5369..6163 
                     /label="Kan/Neo"  
     misc_feature    6198..6500 
                     /label="SV40 ori"  
     misc_feature    6586..7063 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3267..3273 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3267 
                     /label="DLG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAACGGCCA AGTGTTTCTC GGGCTGAGAA TTATCAGCTT TTGTGGGATA 
     1081 CTATTGCTTC CTTAAAACAA TGTGAACAAG CTATGCAACA TGCATTTATT CCGGTCAATG 
     1141 GGACAGAAAT TGAATATGAA TTTGAAGAAA TTACACTGGA GAGGGGGAAT TCTGGCCTGG 
     1201 GATTCAGTAT TGCTGGGGGG ACAGATAATC CCCACATTGG AGATGACCCT GGCATATTTA 
     1261 TTACGAAGAT TATACCAGGA GGTGCTGCAG CAGAGGATGG CAGACTCAGG GTCAATGATT 
     1321 GTATCTTGCG GGTGAATGAG GTTGATGTGT CAGAGGTTTC CCACAGTAAA GCGGTGGAAG 
     1381 CCCTGAAGGA AGCAGGGTCT ATCGTTCGGC TGTATGTGCG TAGAAGACGA CCTATTTTGG 
     1441 AGACCGTTGT GGAAATCAAA CTGTTCAAAG GCCCTAAAGG TTTAGGCTTC AGTATTGCAG 
     1501 GAGGTGTGGG GAACCAACAC ATTCCTGGAG ACAACAGCAT TTATGTAACT AAAATTATAG 
     1561 ATGGAGGAGC TGCACAAAAA GATGGAAGGT TGCAAGTAGG AGATAGACTA CTAATGGTAA 
     1621 ACAACTACAG TTTAGAAGAA GTAACACACG AAGAGGCAGT AGCAATATTA AAGAACACAT 
     1681 CAGAGGTAGT TTATTTAAAA GTTGGCAAAC CCACTACCAT TTATATGACT GATCCTTATG 
     1741 GTCCACCTGA TATTACTCAC TCTTATTCTC CACCAATGGA AAACCATCTA CTCTCTGGCA 
     1801 ACAATGGCAC TTTAGAATAT AAAACCTCCC TGCCACCCAT CTCTCCAGGA AGGTACTCAC 
     1861 CAATTCCAAA GCACATGCTT GTTGACGACG ACTACACCAG TCATTCCCAA CATAGCACCG 
     1921 CAACTCGTCA GCCTTCAATG ACTCTCCAAC GGGCCGTCTC CCTGGAAGGA GAGCCTCGCA 
     1981 AGGTAGTCCT GCACAAAGGC TCCACTGGCC TGGGCTTCAA CATTGTCGGT GGGGAAGATG 
     2041 GAGAAGGTAT TTTTGTGTCC TTCATTCTGG CTGGTGGACC AGCAGACCTA AGTGGGGAGC 
     2101 TCCAGAGAGG AGACCAGATC CTATCGGTGA ATGGCATTGA CCTCCGTGGT GCATCCCACG 
     2161 AGCAGGCAGC TGCTGCACTA AAGGGGGCTG GACAGACAGT GACGATTATA GCACAATATC 
     2221 AACCTGAAGA TTACGCTCGA TTTGAGGCCA AAATCCATGA CCTACGAGAG CAGATGATGA 
     2281 ACCACAGCAT GAGCTCCGGG TCCGGATCCC TGCGAACCAA TCAGAAACGC TCCCTCTACG 
     2341 TCAGAGCCAT GTTCGACTAC GACAAGAGCA AGGACAGTGG GCTGCCAAGT CAAGGACTTA 
     2401 GTTTTAAATA TGGAGATATT CTCCACGTTA TCAATGCCTC TGATGATGAG TGGTGGCAAG 
     2461 CCAGGAGAGT CATGCTGGAG GGAGACAGTG AGGAGATGGG GGTCATCCCC AGCAAAAGGA 
     2521 GGGTGGAAAG AAAGGAACGT GCCCGATTGA AGACAGTGAA GTTTAATGCC AAACCTGGAG 
     2581 TGATTGATTC GAAAGGGGAC ATCCCCGGAT TAGGTGACGA CGGTTATGGA ACAAAGACTC 
     2641 TGAGAGGACA AGAAGACCTC ATTCTTTCCT ATGAGCCTGT TACAAGGCAG GAAATAAACT 
     2701 ACACCCGGCC GGTGATTATC CTGGGGCCCA TGAAGGATCG GATCAATGAC GACTTGATAT 
     2761 CTGAATTCCC TGATAAATTT GGCTCCTGTG TGCCTCATAC TACGAGGCCA AAGCGAGACT 
     2821 ACGAGGTGGA TGGCAGAGAC TATCACTTTG TCATTTCCAG AGAACAAATG GAGAAAGATA 
     2881 TCCAAGAGCA CAAGTTTATA GAAGCCGGCC AGTACAATGA CAATTTATAT GGAACCAGTG 
     2941 TGCAGTCTGT GAGATTTGTA GCAGAAAGAG GCAAACACTG TATACTTGAT GTATCAGGAA 
     3001 ATGCTATCAA GCGGTTACAA GTTGCCCAGC TCTATCCCAT TGCCATCTTC ATAAAACCCA 
     3061 GGTCTCTGGA ACCTCTTATG GAGATGAATA AGCGTCTAAC AGAGGAACAA GCCAAGAAAA 
     3121 CCTATGATCG AGCAATTAAG CTAGAACAAG AATTTGGAGA ATATTTTACA GCTATTGTCC 
     3181 AAGGAGATAC TTTAGAAGAT ATATATAACC AATGCAAGCT TGTTATTGAA GAGCAATCTG 
     3241 GGCCTTTCAT CTGGATTCCC TCAAAGGAAA AGTTAACGCG TACGCGGCCG CTCGAGCAGA 
     3301 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3361 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3421 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3481 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3541 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3601 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3661 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3721 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3781 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3841 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3901 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3961 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4021 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4081 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4141 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4201 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4261 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4321 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4381 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4441 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4501 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4561 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4621 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4681 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4741 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4801 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4861 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4921 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     4981 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5041 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5101 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5161 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5221 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5281 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5341 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5401 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5461 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5521 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5581 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5641 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5701 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5761 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5821 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5881 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     5941 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6001 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6061 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6121 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6181 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6241 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6301 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6361 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6421 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6481 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6541 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6601 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6661 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6721 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6781 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6841 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6901 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     6961 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7021 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7081 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//