LOCUS       RC227146                8439 bp    DNA     circular  SYN 
DEFINITION  FAM178A (Myc-DDK-tagged)-Human family with sequence similarity 178, member A (FAM178A), transcript variant 2
ACCESSION   NM_001136123
VERSION     NM_001136123.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4596..4687 
                     /label="Myc-DDK"  
     misc_feature    4717..5302 
                     /label="PolyA signal"  
     misc_feature    4774..4793 
                     /label="Restriction Enzyme"  
     misc_feature    5578..6250 
                     /label="ColE1"  
     misc_feature    6680..7474 
                     /label="Kan/Neo"  
     misc_feature    7509..7811 
                     /label="SV40 ori"  
     misc_feature    7897..8374 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4578..4584 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4578 
                     /label="SLF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACAAGGCGC TGCATGCCCG CTAGGCCAGG TTTCCCCTCA TCCCCAGCCC 
     1081 CGGGGTCGTC GCCCCCGCGC TGCCATCTGA GACCCGGTAG TACCGCCCAT GCTGCAGCGG 
     1141 GAAAGAGAAC AGAGAGTCCT GGGGACAGGA AGCAGTCAAT TATAGATTTC TTCAAACCAG 
     1201 CTTCAAAACA AGACAGACAC ATGTTGGATT CACCACAAAA ATCAAACATC AAATATGGAG 
     1261 GAAGTAGATT GTCTATCACT GGGACAGAGC AGTTTGAAAG GAAACTATCC TCACCAAAAG 
     1321 AATCTAAACC CAAAAGGGTG CCACCAGAAA AGAGCCCTAT TATAGAAGCT TTCATGAAAG 
     1381 GTGTTAAAGA GCACCATGAA GATCATGGTA TACATGAGTC ACGTCGGCCT TGTCTGTCAC 
     1441 TAGCCTCCAA ATATTTAGCC AAAGGAACAA ATATCTATGT TCCTTCTTCA TATCACTTGC 
     1501 CAAAGGAGAT GAAGTCACTA AAGAAAAAAC ATCGATCCCC AGAGAGAAGG AAGTCACTAT 
     1561 TCATTCATGA AAATAATGAG AAGAATGATA GAGATCGAGG CAAAACCAAT GCAGACTCCA 
     1621 AAAAGCAGAC CACAGTGGCA GAAGCTGACA TCTTCAATAA CAGCTCCAGA AGCCTTAGCA 
     1681 GCAGGAGCAG CCTGTCCAGG CACCACCCGG AAGAAAGCCC ACTGGGAGCT AAATTCCAGT 
     1741 TGTCACTAGC TTCTTACTGC AGAGAACGAG AACTAAAGAG GTTGAGAAAG GAGCAAATGG 
     1801 AGCAGAGAAT CAACTCCGAG AATTCTTTCT CAGAAGCAAG CAGTCTTTCC TTAAAATCTA 
     1861 GTATAGAAAG AAAATATAAA CCAAGGCAGG AACAAAGGAA ACAGAATGAC ATCATACCTG 
     1921 GAAAAAATAA TCTGTCAAAT GTGGAAAATG GACATCTCTC AAGAAAAAGA TCCTCTTCTG 
     1981 ATTCATGGGA ACCTACTTCA GCAGGCTCTA AGCAGAATAA ATTCCCTGAA AAAAGAAAAA 
     2041 GGAACTCTGT GGACTCAGAT CTGAAAAGCA CAAGAGAATC TATGATACCA AAAGCAAGAG 
     2101 AGTCCTTCCT TGAGAAGCGT CCTGATGGAC CACATCAGAA AGAAAAATTT ATAAAACATA 
     2161 TTGCACTGAA GACACCTGGT GATGTGTTGC GCTTAGAAGA TATATCCAAG GAACCGAGTG 
     2221 ATGAAACTGA TGGCTCTTCT GCAGGCTTGG CACCTTCAAA TTCTGGCAAT TCTGGCCACC 
     2281 ATTCTACCAG GAATAGTGAC CAAATCCAAG TGGCAGGTAC CAAGGAGACT AAGATGCAGA 
     2341 AACCCCACTT ACCTTTATCT CAGGAAAAGT CTGCAATTAA AAAAGCTAGC AACCTTCAGA 
     2401 AAAATAAAAC CGCTAGCTCC ACGACAAAGG AGAAGGAGAC AAAACTACCT TTACTTTCCC 
     2461 GTGTTCCAAG TGCTGGTTCC TCTCTAGTAC CATTAAATGC TAAAAATTGT GCTCTTCCAG 
     2521 TTTCTAAAAA AGATAAAGAG CGTTCCTCAT CTAAAGAATG TTCTGGGCAT TCTACAGAAT 
     2581 CCACCAAACA CAAGGAACAC AAAGCAAAGA CTAATAAGGC CGATTCTAAT GTATCTTCAG 
     2641 GGAAAATTTC TGGGGGACCT TTGCGCTCAG AATATGGCAC TCCTACAAAG TCTCCCCCTG 
     2701 CTGCTTTGGA AGTTGTGCCA TGTATCCCAA GCCCTGCAGC ACCTTCAGAT AAAGCCCCTT 
     2761 CAGAAGGAGA GAGTTCAGGA AATTCCAATG CAGGTAGCAG TGCACTGAAA AGAAAACTAA 
     2821 GGGGTGATTT TGATAGTGAT GAAGAAAGTT TAGGTTACAA CCTAGACAGT GATGAGGAAG 
     2881 AGGAAACATT AAAGTCACTG GAAGAAATAA TGGCTTTGAA CTTCAATCAG ACTCCTGCAG 
     2941 CTACAGGAAA GCCTCCTGCT CTTTCCAAGG GGCTTAGATC TCAGTCATCA GACTATACAG 
     3001 GACATGTTCA TCCTGGAACT TACACAAATA CCTTAGAACG TCTAGTGAAG GAAATGGAAG 
     3061 ACACACAAAG GCTAGATGAA CTGCAGAAGC AACTACAAGA AGACATAAGG CAAGGCCGAG 
     3121 GCATTAAATC CCCAATCAGA ATTGGAGAAG AAGACAGTAC AGATGATGAG GATGGCCTCT 
     3181 TAGAAGAGCA CAAGGAATTT CTAAAGAAAT TTTCAGTTAC AATTGATGCT ATTCCTGATC 
     3241 ATCATCCAGG TGAAGAAATA TTTAATTTCC TCAATTCTGG AAAAATTTTC AATCAGTATA 
     3301 CCTTGGATTT AAGAGACTCT GGTTTTATTG GACAAAGTGC TGTAGAAAAA CTTATTCTTA 
     3361 AATCGGGAAA AACAGATCAG ATTTTTTTGA CAACACAAGG TTTCCTTACG TCTGCTTATC 
     3421 ACTATGTCCA GTGTCCTGTC CCTGTGTTAA AGTGGCTGTT TCGGATGATG TCAGTTCATA 
     3481 CAGACTGTAT TGTGTCAGTG CAGATTTTAA GTACATTGAT GGAAATAACA ATTAGAAATG 
     3541 ATACCTTCAG TGACTCACCA GTTTGGCCAT GGATCCCATC ATTGTCTGAT GTAGCAGCTG 
     3601 TGTTTTTCAA TATGGGGATT GATTTTAGAT CTTTGTTTCC CCTGGAGAAT CTTCAGCCAG 
     3661 ACTTTAATGA AGACTATCTA GTTTCTGAAA CACAGACAAC ATCAAGGGGG AAAGAAAGTG 
     3721 AAGATTCATC TTATAAGCCA ATTTTTTCAA CACTTCCTGA AACCAACATT TTAAATGTGG 
     3781 TTAAGTTTCT AGGCTTGTGT ACATCTATAC ATCCAGAAGG TTACCAGGAT CGTGAAATAA 
     3841 TGTTGCTGAT TTTAATGTTA TTTAAAATGA GTTTGGAAAA ACAGCTGAAA CAGATTCCTT 
     3901 TAGTAGACTT TCAAAGCCTC CTGATAAACC TGATGAAAAA CATCAGAGAT TGGAACACAA 
     3961 AGGTGCCTGA ACTCTGTCTG GGCATAAATG AACTCTCCAG TCATCCCCAC AACCTCCTGT 
     4021 GGTTGGTACA GCTGGTCCCT AATTGGACAT CACGTGGAAG GCAACTGAGA CAGTGCCTCA 
     4081 GTCTAGTGAT TATTTCAAAG CTTTTGGATG AGAAACACGA AGATGTTCCT AATGCCAGTA 
     4141 ATCTGCAGGT ATCAGTCCTA CATCGCTATC TTGTGCAGAT GAAGCCTTCT GATTTGTTAA 
     4201 AGAAAATGGT CTTGAAGAAA AAGGCTGAAC AACCAGATGG CATTATTGAT GACAGTCTTC 
     4261 ATTTAGAACT TGAAAAGCAG GCATATTACC TGACCTACAT TCTTCTTCAT TTAGTCGGTG 
     4321 AAGTTAGTTG TTCTCATTCT TTTTCTTCTG GACAACGGAA ACACTTTGTG CTACTCTGTG 
     4381 GGGCTTTGGA AAAGCATGTT AAATGTGATA TTAGGGAAGA TGCAAGACTT TTTTACAGAA 
     4441 CTAAGGTGAA AGACTTGGTC GCCAGGATAC ATGGAAAATG GCAGGAAATA ATCCAGAACT 
     4501 GTCGGCCTAC TCAGGTGTCA TTTTGTTATA CAATTTCATG TATTCTTAAT AGTTTTGCTG 
     4561 AATGGCACTC TTCATACTGT TTGAAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4621 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4681 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4741 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4801 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4861 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4921 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4981 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5041 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5101 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5161 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5221 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5281 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5341 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5401 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5461 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5521 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5581 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5641 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5701 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5761 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5821 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5881 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5941 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6001 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6061 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6121 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6181 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6241 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6301 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6361 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6421 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6481 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6541 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6601 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6661 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6721 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6781 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6841 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6901 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6961 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7021 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7081 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7141 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7201 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7261 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7321 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7381 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7441 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7501 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7561 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7621 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7681 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7861 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7921 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7981 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8041 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8101 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8161 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8221 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8281 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8341 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8401 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//